Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07275 and MMJJ_RS07280 are separated by 63 nucleotides MMJJ_RS07280 and MMJJ_RS07285 are separated by 286 nucleotides MMJJ_RS07285 and MMJJ_RS07290 are separated by 48 nucleotides
MMJJ_RS07275: MMJJ_RS07275 - formate--phosphoribosylaminoimidazolecarboxamide ligase, at 1,332,540 to 1,333,625
_RS07275
MMJJ_RS07280: MMJJ_RS07280 - phosphomannomutase/phosphoglucomutase, at 1,333,689 to 1,335,002
_RS07280
MMJJ_RS07285: MMJJ_RS07285 - 30S ribosomal protein S10, at 1,335,289 to 1,335,597
_RS07285
MMJJ_RS07290: MMJJ_RS07290 - translation elongation factor EF-1 subunit alpha, at 1,335,646 to 1,336,932
_RS07290
Position (kb)
1333
1334
1335
1336 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1332.696 kb on + strand, within MMJJ_RS07275 at 1332.735 kb on - strand, within MMJJ_RS07275 at 1332.736 kb on + strand, within MMJJ_RS07275 at 1333.205 kb on + strand, within MMJJ_RS07275 at 1333.217 kb on - strand, within MMJJ_RS07275 at 1333.324 kb on - strand, within MMJJ_RS07275 at 1333.436 kb on + strand, within MMJJ_RS07275 at 1333.436 kb on + strand, within MMJJ_RS07275 at 1333.505 kb on - strand, within MMJJ_RS07275 at 1333.617 kb on + strand at 1333.623 kb on - strand at 1333.624 kb on + strand at 1333.624 kb on + strand at 1333.659 kb on + strand at 1333.673 kb on - strand at 1333.679 kb on - strand at 1333.698 kb on - strand at 1333.719 kb on - strand at 1333.815 kb on + strand at 1334.002 kb on - strand, within MMJJ_RS07280 at 1334.013 kb on - strand, within MMJJ_RS07280 at 1334.050 kb on - strand, within MMJJ_RS07280 at 1334.116 kb on - strand, within MMJJ_RS07280 at 1334.116 kb on - strand, within MMJJ_RS07280 at 1334.117 kb on + strand, within MMJJ_RS07280 at 1334.136 kb on - strand, within MMJJ_RS07280 at 1334.253 kb on - strand, within MMJJ_RS07280 at 1334.254 kb on + strand, within MMJJ_RS07280 at 1334.254 kb on + strand, within MMJJ_RS07280 at 1334.358 kb on - strand, within MMJJ_RS07280 at 1334.358 kb on - strand, within MMJJ_RS07280 at 1334.385 kb on + strand, within MMJJ_RS07280 at 1334.440 kb on + strand, within MMJJ_RS07280 at 1334.444 kb on - strand, within MMJJ_RS07280 at 1334.444 kb on - strand, within MMJJ_RS07280 at 1334.568 kb on - strand, within MMJJ_RS07280 at 1334.667 kb on - strand, within MMJJ_RS07280 at 1334.716 kb on - strand, within MMJJ_RS07280 at 1334.716 kb on - strand, within MMJJ_RS07280 at 1334.782 kb on - strand, within MMJJ_RS07280 at 1334.782 kb on - strand, within MMJJ_RS07280 at 1334.782 kb on - strand, within MMJJ_RS07280 at 1334.782 kb on - strand, within MMJJ_RS07280 at 1334.783 kb on + strand, within MMJJ_RS07280 at 1334.835 kb on - strand, within MMJJ_RS07280 at 1334.847 kb on + strand, within MMJJ_RS07280 at 1334.956 kb on + strand at 1335.045 kb on - strand at 1335.047 kb on - strand at 1335.047 kb on - strand at 1335.047 kb on - strand at 1335.048 kb on + strand at 1335.048 kb on + strand at 1335.048 kb on + strand at 1335.048 kb on + strand at 1335.051 kb on + strand at 1335.051 kb on + strand at 1335.051 kb on + strand at 1335.092 kb on - strand at 1335.095 kb on - strand at 1335.096 kb on + strand at 1335.098 kb on + strand at 1335.103 kb on + strand at 1335.103 kb on + strand at 1335.107 kb on + strand at 1335.135 kb on + strand at 1335.160 kb on - strand at 1335.160 kb on - strand at 1335.161 kb on + strand at 1335.163 kb on - strand at 1335.163 kb on - strand at 1335.164 kb on + strand at 1335.164 kb on + strand at 1335.167 kb on - strand at 1335.171 kb on + strand at 1335.171 kb on + strand at 1335.172 kb on - strand at 1335.173 kb on + strand at 1335.175 kb on - strand at 1335.178 kb on - strand at 1335.180 kb on - strand at 1335.181 kb on + strand at 1335.601 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 1,332,696 + MMJJ_RS07275 0.14 -1.0 1,332,735 - MMJJ_RS07275 0.18 +0.2 1,332,736 + MMJJ_RS07275 0.18 -0.0 1,333,205 + MMJJ_RS07275 0.61 -0.3 1,333,217 - MMJJ_RS07275 0.62 -0.3 1,333,324 - MMJJ_RS07275 0.72 +2.4 1,333,436 + MMJJ_RS07275 0.83 -2.3 1,333,436 + MMJJ_RS07275 0.83 -1.0 1,333,505 - MMJJ_RS07275 0.89 -0.6 1,333,617 + +0.4 1,333,623 - -1.1 1,333,624 + -0.1 1,333,624 + +0.1 1,333,659 + -0.5 1,333,673 - +0.0 1,333,679 - +0.7 1,333,698 - +0.1 1,333,719 - -1.7 1,333,815 + -0.7 1,334,002 - MMJJ_RS07280 0.24 -0.7 1,334,013 - MMJJ_RS07280 0.25 -2.6 1,334,050 - MMJJ_RS07280 0.27 -2.1 1,334,116 - MMJJ_RS07280 0.32 -2.3 1,334,116 - MMJJ_RS07280 0.32 -1.6 1,334,117 + MMJJ_RS07280 0.33 -1.2 1,334,136 - MMJJ_RS07280 0.34 -0.6 1,334,253 - MMJJ_RS07280 0.43 -0.3 1,334,254 + MMJJ_RS07280 0.43 -1.1 1,334,254 + MMJJ_RS07280 0.43 -0.4 1,334,358 - MMJJ_RS07280 0.51 -1.3 1,334,358 - MMJJ_RS07280 0.51 +0.2 1,334,385 + MMJJ_RS07280 0.53 -2.5 1,334,440 + MMJJ_RS07280 0.57 -2.3 1,334,444 - MMJJ_RS07280 0.57 -2.5 1,334,444 - MMJJ_RS07280 0.57 -3.2 1,334,568 - MMJJ_RS07280 0.67 -2.5 1,334,667 - MMJJ_RS07280 0.74 -0.6 1,334,716 - MMJJ_RS07280 0.78 -2.0 1,334,716 - MMJJ_RS07280 0.78 -0.3 1,334,782 - MMJJ_RS07280 0.83 -3.7 1,334,782 - MMJJ_RS07280 0.83 -2.8 1,334,782 - MMJJ_RS07280 0.83 -1.7 1,334,782 - MMJJ_RS07280 0.83 +0.2 1,334,783 + MMJJ_RS07280 0.83 -1.6 1,334,835 - MMJJ_RS07280 0.87 -3.5 1,334,847 + MMJJ_RS07280 0.88 -2.1 1,334,956 + -1.9 1,335,045 - +2.0 1,335,047 - +1.0 1,335,047 - +0.7 1,335,047 - +0.8 1,335,048 + +1.0 1,335,048 + -0.8 1,335,048 + -0.5 1,335,048 + +0.8 1,335,051 + +2.2 1,335,051 + +0.3 1,335,051 + -0.2 1,335,092 - +2.6 1,335,095 - +1.4 1,335,096 + -1.0 1,335,098 + -0.6 1,335,103 + +0.2 1,335,103 + -0.2 1,335,107 + -1.1 1,335,135 + +0.1 1,335,160 - +0.3 1,335,160 - +1.0 1,335,161 + +0.1 1,335,163 - +0.2 1,335,163 - -1.4 1,335,164 + -1.5 1,335,164 + -0.7 1,335,167 - -0.6 1,335,171 + +1.1 1,335,171 + +0.2 1,335,172 - -0.7 1,335,173 + +0.8 1,335,175 - +0.7 1,335,178 - +0.3 1,335,180 - +1.2 1,335,181 + +1.0 1,335,601 + -0.8
Or see this region's nucleotide sequence