Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05155 and MMJJ_RS05160 are separated by 107 nucleotides MMJJ_RS05160 and MMJJ_RS05165 are separated by 13 nucleotides MMJJ_RS05165 and MMJJ_RS05170 overlap by 4 nucleotides MMJJ_RS05170 and MMJJ_RS05175 are separated by 130 nucleotides
MMJJ_RS05155: MMJJ_RS05155 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 954,476 to 955,555
_RS05155
MMJJ_RS05160: MMJJ_RS05160 - RNA-binding protein, at 955,663 to 956,142
_RS05160
MMJJ_RS05165: MMJJ_RS05165 - class I SAM-dependent methyltransferase, at 956,156 to 956,764
_RS05165
MMJJ_RS05170: MMJJ_RS05170 - Nif3-like dinuclear metal center hexameric protein, at 956,761 to 957,501
_RS05170
MMJJ_RS05175: MMJJ_RS05175 - CBS domain-containing protein, at 957,632 to 958,543
_RS05175
Position (kb) 956
957 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.371 kb on - strand, within MMJJ_RS05155 at 955.467 kb on + strand at 955.467 kb on + strand at 955.500 kb on - strand at 955.549 kb on + strand at 955.553 kb on - strand at 955.553 kb on - strand at 955.553 kb on - strand at 955.553 kb on - strand at 955.554 kb on + strand at 955.562 kb on - strand at 955.563 kb on + strand at 955.563 kb on + strand at 955.591 kb on - strand at 955.592 kb on + strand at 955.597 kb on - strand at 955.598 kb on + strand at 955.598 kb on + strand at 955.598 kb on + strand at 955.601 kb on - strand at 955.602 kb on + strand at 955.651 kb on + strand at 955.681 kb on - strand at 955.681 kb on - strand at 955.682 kb on + strand at 955.682 kb on + strand at 955.850 kb on - strand at 955.851 kb on + strand, within MMJJ_RS05160 at 955.902 kb on - strand, within MMJJ_RS05160 at 955.907 kb on - strand, within MMJJ_RS05160 at 955.985 kb on - strand, within MMJJ_RS05160 at 956.161 kb on + strand at 956.190 kb on - strand at 956.191 kb on + strand at 956.203 kb on - strand at 956.208 kb on - strand at 956.239 kb on + strand, within MMJJ_RS05165 at 956.342 kb on + strand, within MMJJ_RS05165 at 956.355 kb on - strand, within MMJJ_RS05165 at 956.355 kb on - strand, within MMJJ_RS05165 at 956.428 kb on - strand, within MMJJ_RS05165 at 956.428 kb on - strand, within MMJJ_RS05165 at 956.429 kb on + strand, within MMJJ_RS05165 at 956.452 kb on - strand, within MMJJ_RS05165 at 956.473 kb on + strand, within MMJJ_RS05165 at 956.478 kb on - strand, within MMJJ_RS05165 at 956.492 kb on - strand, within MMJJ_RS05165 at 956.493 kb on + strand, within MMJJ_RS05165 at 956.587 kb on + strand, within MMJJ_RS05165 at 956.596 kb on + strand, within MMJJ_RS05165 at 956.603 kb on - strand, within MMJJ_RS05165 at 956.603 kb on - strand, within MMJJ_RS05165 at 956.604 kb on + strand, within MMJJ_RS05165 at 956.660 kb on + strand, within MMJJ_RS05165 at 956.737 kb on + strand at 956.781 kb on + strand at 956.852 kb on - strand, within MMJJ_RS05170 at 957.017 kb on - strand, within MMJJ_RS05170 at 957.020 kb on - strand, within MMJJ_RS05170 at 957.020 kb on - strand, within MMJJ_RS05170 at 957.025 kb on + strand, within MMJJ_RS05170 at 957.054 kb on - strand, within MMJJ_RS05170 at 957.054 kb on - strand, within MMJJ_RS05170 at 957.067 kb on + strand, within MMJJ_RS05170 at 957.085 kb on + strand, within MMJJ_RS05170 at 957.095 kb on - strand, within MMJJ_RS05170 at 957.108 kb on - strand, within MMJJ_RS05170 at 957.134 kb on + strand, within MMJJ_RS05170 at 957.184 kb on + strand, within MMJJ_RS05170 at 957.207 kb on - strand, within MMJJ_RS05170 at 957.208 kb on + strand, within MMJJ_RS05170 at 957.237 kb on - strand, within MMJJ_RS05170 at 957.245 kb on - strand, within MMJJ_RS05170 at 957.245 kb on - strand, within MMJJ_RS05170 at 957.245 kb on - strand, within MMJJ_RS05170 at 957.245 kb on - strand, within MMJJ_RS05170 at 957.247 kb on - strand, within MMJJ_RS05170 at 957.247 kb on - strand, within MMJJ_RS05170 at 957.248 kb on + strand, within MMJJ_RS05170 at 957.248 kb on + strand, within MMJJ_RS05170 at 957.248 kb on + strand, within MMJJ_RS05170 at 957.248 kb on + strand, within MMJJ_RS05170 at 957.307 kb on + strand, within MMJJ_RS05170 at 957.309 kb on - strand, within MMJJ_RS05170 at 957.313 kb on - strand, within MMJJ_RS05170 at 957.313 kb on - strand, within MMJJ_RS05170 at 957.313 kb on - strand, within MMJJ_RS05170 at 957.313 kb on - strand, within MMJJ_RS05170 at 957.313 kb on - strand, within MMJJ_RS05170 at 957.314 kb on + strand, within MMJJ_RS05170 at 957.337 kb on - strand, within MMJJ_RS05170 at 957.337 kb on - strand, within MMJJ_RS05170 at 957.386 kb on - strand, within MMJJ_RS05170 at 957.387 kb on + strand, within MMJJ_RS05170 at 957.455 kb on + strand at 957.455 kb on + strand at 957.505 kb on - strand at 957.533 kb on + strand at 957.536 kb on - strand at 957.536 kb on - strand at 957.555 kb on + strand at 957.555 kb on + strand at 957.578 kb on - strand at 957.578 kb on - strand at 957.579 kb on + strand at 957.579 kb on + strand at 957.579 kb on + strand at 957.579 kb on + strand at 957.579 kb on + strand at 957.579 kb on + strand at 957.579 kb on + strand at 957.580 kb on - strand at 957.580 kb on - strand at 957.580 kb on - strand at 957.580 kb on - strand at 957.580 kb on - strand at 957.581 kb on + strand at 957.581 kb on + strand at 957.581 kb on + strand at 957.581 kb on + strand at 957.581 kb on + strand at 957.582 kb on - strand at 957.582 kb on - strand at 957.582 kb on - strand at 957.583 kb on + strand at 957.594 kb on - strand at 957.594 kb on - strand at 957.595 kb on + strand at 957.596 kb on - strand at 957.597 kb on + strand at 957.597 kb on + strand at 957.607 kb on - strand at 957.627 kb on - strand at 957.627 kb on - strand at 957.628 kb on + strand at 957.628 kb on + strand at 957.631 kb on - strand at 957.641 kb on + strand at 957.641 kb on + strand at 957.641 kb on + strand at 957.645 kb on - strand at 957.645 kb on - strand at 957.646 kb on + strand at 957.661 kb on + strand at 957.727 kb on - strand, within MMJJ_RS05175 at 957.733 kb on + strand, within MMJJ_RS05175 at 957.737 kb on + strand, within MMJJ_RS05175
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 955,325 - MMJJ_RS05155 0.79 +0.2 955,325 - MMJJ_RS05155 0.79 -0.9 955,325 - MMJJ_RS05155 0.79 +0.3 955,325 - MMJJ_RS05155 0.79 -0.9 955,371 - MMJJ_RS05155 0.83 -1.7 955,467 + -0.0 955,467 + -1.0 955,500 - -1.1 955,549 + +0.7 955,553 - +1.7 955,553 - +0.4 955,553 - -0.3 955,553 - -0.0 955,554 + -3.5 955,562 - +0.0 955,563 + +1.6 955,563 + -1.5 955,591 - -0.2 955,592 + -0.5 955,597 - -1.5 955,598 + -0.8 955,598 + -0.6 955,598 + -1.2 955,601 - -0.9 955,602 + -0.4 955,651 + +2.3 955,681 - -0.1 955,681 - +0.0 955,682 + +0.9 955,682 + +0.7 955,850 - +1.5 955,851 + MMJJ_RS05160 0.39 -0.8 955,902 - MMJJ_RS05160 0.50 -2.2 955,907 - MMJJ_RS05160 0.51 +0.0 955,985 - MMJJ_RS05160 0.67 -1.4 956,161 + +0.6 956,190 - -0.6 956,191 + -2.0 956,203 - -1.0 956,208 - +0.4 956,239 + MMJJ_RS05165 0.14 -0.5 956,342 + MMJJ_RS05165 0.31 -3.4 956,355 - MMJJ_RS05165 0.33 +1.4 956,355 - MMJJ_RS05165 0.33 -1.6 956,428 - MMJJ_RS05165 0.45 -1.3 956,428 - MMJJ_RS05165 0.45 -2.2 956,429 + MMJJ_RS05165 0.45 -0.2 956,452 - MMJJ_RS05165 0.49 -0.7 956,473 + MMJJ_RS05165 0.52 -1.1 956,478 - MMJJ_RS05165 0.53 -0.6 956,492 - MMJJ_RS05165 0.55 -1.3 956,493 + MMJJ_RS05165 0.55 -0.3 956,587 + MMJJ_RS05165 0.71 -1.3 956,596 + MMJJ_RS05165 0.72 -1.0 956,603 - MMJJ_RS05165 0.73 +0.0 956,603 - MMJJ_RS05165 0.73 -1.0 956,604 + MMJJ_RS05165 0.74 +0.0 956,660 + MMJJ_RS05165 0.83 -2.7 956,737 + -2.6 956,781 + +0.4 956,852 - MMJJ_RS05170 0.12 -1.7 957,017 - MMJJ_RS05170 0.35 -2.0 957,020 - MMJJ_RS05170 0.35 +0.4 957,020 - MMJJ_RS05170 0.35 -1.7 957,025 + MMJJ_RS05170 0.36 -0.3 957,054 - MMJJ_RS05170 0.40 +1.5 957,054 - MMJJ_RS05170 0.40 -0.9 957,067 + MMJJ_RS05170 0.41 -1.2 957,085 + MMJJ_RS05170 0.44 -0.9 957,095 - MMJJ_RS05170 0.45 +0.6 957,108 - MMJJ_RS05170 0.47 -0.4 957,134 + MMJJ_RS05170 0.50 -0.5 957,184 + MMJJ_RS05170 0.57 -0.0 957,207 - MMJJ_RS05170 0.60 +0.2 957,208 + MMJJ_RS05170 0.60 -0.9 957,237 - MMJJ_RS05170 0.64 +0.0 957,245 - MMJJ_RS05170 0.65 -1.2 957,245 - MMJJ_RS05170 0.65 -2.0 957,245 - MMJJ_RS05170 0.65 +0.2 957,245 - MMJJ_RS05170 0.65 -1.5 957,247 - MMJJ_RS05170 0.66 +1.0 957,247 - MMJJ_RS05170 0.66 +0.2 957,248 + MMJJ_RS05170 0.66 +1.2 957,248 + MMJJ_RS05170 0.66 -1.1 957,248 + MMJJ_RS05170 0.66 +1.3 957,248 + MMJJ_RS05170 0.66 -1.4 957,307 + MMJJ_RS05170 0.74 -0.4 957,309 - MMJJ_RS05170 0.74 -2.4 957,313 - MMJJ_RS05170 0.74 -0.6 957,313 - MMJJ_RS05170 0.74 +1.0 957,313 - MMJJ_RS05170 0.74 +0.0 957,313 - MMJJ_RS05170 0.74 +0.8 957,313 - MMJJ_RS05170 0.74 -0.7 957,314 + MMJJ_RS05170 0.75 -1.0 957,337 - MMJJ_RS05170 0.78 -1.9 957,337 - MMJJ_RS05170 0.78 -0.7 957,386 - MMJJ_RS05170 0.84 -0.8 957,387 + MMJJ_RS05170 0.84 -1.0 957,455 + +0.6 957,455 + -0.0 957,505 - +0.9 957,533 + -0.3 957,536 - +1.4 957,536 - +0.5 957,555 + -0.9 957,555 + -1.9 957,578 - -2.8 957,578 - -3.0 957,579 + -1.2 957,579 + -0.6 957,579 + -2.1 957,579 + -0.2 957,579 + -0.8 957,579 + +0.8 957,579 + -1.6 957,580 - +0.2 957,580 - -0.4 957,580 - -1.7 957,580 - -2.3 957,580 - +1.8 957,581 + -3.1 957,581 + -2.0 957,581 + -3.6 957,581 + -3.0 957,581 + -2.1 957,582 - -0.8 957,582 - -1.6 957,582 - -3.3 957,583 + -1.7 957,594 - -2.4 957,594 - -1.6 957,595 + -2.0 957,596 - -2.6 957,597 + -1.7 957,597 + -3.9 957,607 - -1.6 957,627 - -1.6 957,627 - -4.5 957,628 + -2.2 957,628 + -3.0 957,631 - -3.1 957,641 + -2.0 957,641 + -0.2 957,641 + -2.2 957,645 - -1.2 957,645 - -3.4 957,646 + -2.5 957,661 + -3.2 957,727 - MMJJ_RS05175 0.10 -0.9 957,733 + MMJJ_RS05175 0.11 -2.6 957,737 + MMJJ_RS05175 0.12 -3.0
Or see this region's nucleotide sequence