Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00335 and MMJJ_RS00340 are separated by 10 nucleotides MMJJ_RS00340 and MMJJ_RS00345 are separated by 8 nucleotides MMJJ_RS00345 and MMJJ_RS00350 are separated by 8 nucleotides MMJJ_RS00350 and MMJJ_RS00355 are separated by 113 nucleotides
MMJJ_RS00335: MMJJ_RS00335 - chemotaxis protein CheW, at 65,256 to 65,699
_RS00335
MMJJ_RS00340: MMJJ_RS00340 - tRNA (pseudouridine(54)-N(1))-methyltransferase TrmY, at 65,710 to 66,306
_RS00340
MMJJ_RS00345: MMJJ_RS00345 - 4-hydroxy-tetrahydrodipicolinate reductase, at 66,315 to 67,127
_RS00345
MMJJ_RS00350: MMJJ_RS00350 - phage infection protein, at 67,136 to 67,786
_RS00350
MMJJ_RS00355: MMJJ_RS00355 - ribonuclease P protein component 4, at 67,900 to 68,232
_RS00355
Position (kb) 66
67
68 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 65.338 kb on - strand, within MMJJ_RS00335 at 65.338 kb on - strand, within MMJJ_RS00335 at 65.404 kb on + strand, within MMJJ_RS00335 at 65.427 kb on - strand, within MMJJ_RS00335 at 65.463 kb on - strand, within MMJJ_RS00335 at 65.463 kb on - strand, within MMJJ_RS00335 at 65.464 kb on + strand, within MMJJ_RS00335 at 65.542 kb on + strand, within MMJJ_RS00335 at 65.562 kb on + strand, within MMJJ_RS00335 at 65.637 kb on - strand, within MMJJ_RS00335 at 65.680 kb on + strand at 65.721 kb on - strand at 65.721 kb on - strand at 65.722 kb on + strand at 65.726 kb on + strand at 65.726 kb on + strand at 65.726 kb on + strand at 65.734 kb on + strand at 65.746 kb on - strand at 65.746 kb on - strand at 65.747 kb on + strand at 65.747 kb on + strand at 65.747 kb on + strand at 65.785 kb on - strand, within MMJJ_RS00340 at 65.912 kb on + strand, within MMJJ_RS00340 at 65.959 kb on + strand, within MMJJ_RS00340 at 65.959 kb on + strand, within MMJJ_RS00340 at 65.962 kb on + strand, within MMJJ_RS00340 at 66.141 kb on - strand, within MMJJ_RS00340 at 66.142 kb on + strand, within MMJJ_RS00340 at 66.156 kb on - strand, within MMJJ_RS00340 at 66.212 kb on + strand, within MMJJ_RS00340 at 66.288 kb on - strand at 66.346 kb on - strand at 66.346 kb on - strand at 66.347 kb on + strand at 66.350 kb on - strand at 66.351 kb on + strand at 66.377 kb on - strand at 66.377 kb on - strand at 66.378 kb on + strand at 66.462 kb on - strand, within MMJJ_RS00345 at 66.463 kb on + strand, within MMJJ_RS00345 at 66.580 kb on + strand, within MMJJ_RS00345 at 66.710 kb on - strand, within MMJJ_RS00345 at 66.717 kb on - strand, within MMJJ_RS00345 at 66.718 kb on + strand, within MMJJ_RS00345 at 66.719 kb on - strand, within MMJJ_RS00345 at 66.719 kb on - strand, within MMJJ_RS00345 at 66.719 kb on - strand, within MMJJ_RS00345 at 66.720 kb on + strand, within MMJJ_RS00345 at 66.720 kb on + strand, within MMJJ_RS00345 at 66.720 kb on + strand, within MMJJ_RS00345 at 66.720 kb on + strand, within MMJJ_RS00345 at 66.723 kb on - strand, within MMJJ_RS00345 at 66.723 kb on - strand, within MMJJ_RS00345 at 66.723 kb on - strand, within MMJJ_RS00345 at 66.724 kb on + strand, within MMJJ_RS00345 at 66.818 kb on - strand, within MMJJ_RS00345 at 66.818 kb on - strand, within MMJJ_RS00345 at 66.827 kb on - strand, within MMJJ_RS00345 at 66.900 kb on - strand, within MMJJ_RS00345 at 66.904 kb on + strand, within MMJJ_RS00345 at 66.904 kb on + strand, within MMJJ_RS00345 at 66.950 kb on - strand, within MMJJ_RS00345 at 66.951 kb on + strand, within MMJJ_RS00345 at 66.954 kb on - strand, within MMJJ_RS00345 at 66.961 kb on + strand, within MMJJ_RS00345 at 67.041 kb on - strand, within MMJJ_RS00345 at 67.041 kb on - strand, within MMJJ_RS00345 at 67.041 kb on - strand, within MMJJ_RS00345 at 67.041 kb on - strand, within MMJJ_RS00345 at 67.042 kb on + strand, within MMJJ_RS00345 at 67.047 kb on - strand at 67.047 kb on - strand at 67.048 kb on + strand at 67.110 kb on + strand at 67.171 kb on + strand at 67.199 kb on - strand at 67.199 kb on - strand at 67.200 kb on + strand at 67.200 kb on + strand at 67.221 kb on + strand, within MMJJ_RS00350 at 67.234 kb on - strand, within MMJJ_RS00350 at 67.235 kb on + strand, within MMJJ_RS00350 at 67.245 kb on + strand, within MMJJ_RS00350 at 67.256 kb on - strand, within MMJJ_RS00350 at 67.256 kb on - strand, within MMJJ_RS00350 at 67.257 kb on + strand, within MMJJ_RS00350 at 67.257 kb on + strand, within MMJJ_RS00350 at 67.257 kb on + strand, within MMJJ_RS00350 at 67.276 kb on + strand, within MMJJ_RS00350 at 67.296 kb on + strand, within MMJJ_RS00350 at 67.357 kb on - strand, within MMJJ_RS00350 at 67.363 kb on - strand, within MMJJ_RS00350 at 67.363 kb on - strand, within MMJJ_RS00350 at 67.363 kb on - strand, within MMJJ_RS00350 at 67.364 kb on + strand, within MMJJ_RS00350 at 67.364 kb on + strand, within MMJJ_RS00350 at 67.364 kb on + strand, within MMJJ_RS00350 at 67.364 kb on + strand, within MMJJ_RS00350 at 67.371 kb on + strand, within MMJJ_RS00350 at 67.373 kb on - strand, within MMJJ_RS00350 at 67.373 kb on - strand, within MMJJ_RS00350 at 67.373 kb on - strand, within MMJJ_RS00350 at 67.374 kb on + strand, within MMJJ_RS00350 at 67.374 kb on + strand, within MMJJ_RS00350 at 67.394 kb on + strand, within MMJJ_RS00350 at 67.397 kb on - strand, within MMJJ_RS00350 at 67.397 kb on - strand, within MMJJ_RS00350 at 67.397 kb on - strand, within MMJJ_RS00350 at 67.397 kb on - strand, within MMJJ_RS00350 at 67.398 kb on + strand, within MMJJ_RS00350 at 67.398 kb on + strand, within MMJJ_RS00350 at 67.403 kb on - strand, within MMJJ_RS00350 at 67.404 kb on + strand, within MMJJ_RS00350 at 67.404 kb on + strand at 67.404 kb on + strand, within MMJJ_RS00350 at 67.408 kb on - strand, within MMJJ_RS00350 at 67.408 kb on - strand, within MMJJ_RS00350 at 67.409 kb on + strand, within MMJJ_RS00350 at 67.409 kb on + strand, within MMJJ_RS00350 at 67.433 kb on - strand, within MMJJ_RS00350 at 67.437 kb on + strand, within MMJJ_RS00350 at 67.439 kb on - strand, within MMJJ_RS00350 at 67.439 kb on - strand, within MMJJ_RS00350 at 67.439 kb on - strand, within MMJJ_RS00350 at 67.439 kb on - strand, within MMJJ_RS00350 at 67.439 kb on - strand, within MMJJ_RS00350 at 67.440 kb on + strand, within MMJJ_RS00350 at 67.451 kb on + strand, within MMJJ_RS00350 at 67.451 kb on + strand, within MMJJ_RS00350 at 67.451 kb on + strand, within MMJJ_RS00350 at 67.453 kb on - strand at 67.457 kb on + strand, within MMJJ_RS00350 at 67.474 kb on - strand, within MMJJ_RS00350 at 67.474 kb on - strand, within MMJJ_RS00350 at 67.555 kb on - strand, within MMJJ_RS00350 at 67.556 kb on + strand, within MMJJ_RS00350 at 67.581 kb on - strand, within MMJJ_RS00350 at 67.581 kb on - strand, within MMJJ_RS00350 at 67.581 kb on - strand, within MMJJ_RS00350 at 67.581 kb on - strand, within MMJJ_RS00350 at 67.620 kb on - strand, within MMJJ_RS00350 at 67.670 kb on + strand, within MMJJ_RS00350 at 67.670 kb on + strand, within MMJJ_RS00350 at 67.670 kb on + strand, within MMJJ_RS00350 at 67.671 kb on - strand, within MMJJ_RS00350 at 67.671 kb on - strand, within MMJJ_RS00350 at 67.672 kb on + strand, within MMJJ_RS00350 at 67.673 kb on - strand, within MMJJ_RS00350 at 67.703 kb on + strand, within MMJJ_RS00350 at 67.703 kb on + strand, within MMJJ_RS00350 at 67.768 kb on - strand at 67.771 kb on - strand at 67.801 kb on - strand at 67.801 kb on - strand at 67.801 kb on - strand at 67.802 kb on + strand at 67.803 kb on - strand at 67.803 kb on - strand at 67.803 kb on - strand at 67.804 kb on + strand at 67.804 kb on + strand at 67.804 kb on + strand at 67.804 kb on + strand at 67.804 kb on + strand at 67.808 kb on - strand at 67.808 kb on - strand at 67.808 kb on - strand at 67.809 kb on + strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.829 kb on - strand at 67.829 kb on - strand at 67.829 kb on - strand at 67.829 kb on - strand at 67.830 kb on + strand at 67.830 kb on + strand at 67.830 kb on + strand at 67.830 kb on + strand at 67.830 kb on + strand at 67.830 kb on + strand at 67.831 kb on - strand at 67.831 kb on - strand at 67.831 kb on - strand at 67.831 kb on - strand at 67.832 kb on + strand at 67.832 kb on + strand at 67.832 kb on + strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.836 kb on - strand at 67.836 kb on - strand at 67.837 kb on + strand at 67.837 kb on + strand at 67.837 kb on + strand at 67.837 kb on + strand at 67.837 kb on + strand at 67.849 kb on - strand at 67.849 kb on - strand at 67.850 kb on + strand at 67.853 kb on - strand at 67.853 kb on - strand at 67.853 kb on - strand at 67.853 kb on - strand at 67.854 kb on + strand at 67.854 kb on + strand at 67.854 kb on + strand at 67.854 kb on + strand at 67.857 kb on - strand at 67.857 kb on - strand at 67.857 kb on - strand at 67.858 kb on + strand at 67.858 kb on + strand at 67.858 kb on + strand at 67.858 kb on + strand at 67.858 kb on + strand at 67.863 kb on - strand at 67.863 kb on - strand at 67.881 kb on - strand at 67.881 kb on - strand at 67.882 kb on + strand at 67.895 kb on - strand at 67.902 kb on + strand at 67.960 kb on - strand, within MMJJ_RS00355 at 68.012 kb on - strand, within MMJJ_RS00355 at 68.014 kb on - strand, within MMJJ_RS00355 at 68.014 kb on - strand, within MMJJ_RS00355 at 68.015 kb on + strand, within MMJJ_RS00355 at 68.108 kb on + strand, within MMJJ_RS00355
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 65,338 - MMJJ_RS00335 0.18 +1.0 65,338 - MMJJ_RS00335 0.18 +0.4 65,404 + MMJJ_RS00335 0.33 -0.4 65,427 - MMJJ_RS00335 0.39 +0.2 65,463 - MMJJ_RS00335 0.47 -0.8 65,463 - MMJJ_RS00335 0.47 -0.3 65,464 + MMJJ_RS00335 0.47 -2.0 65,542 + MMJJ_RS00335 0.64 -0.5 65,562 + MMJJ_RS00335 0.69 -0.6 65,637 - MMJJ_RS00335 0.86 +1.9 65,680 + -0.3 65,721 - +0.8 65,721 - +0.7 65,722 + -0.4 65,726 + -0.3 65,726 + -1.3 65,726 + -1.0 65,734 + -0.9 65,746 - +1.8 65,746 - +0.3 65,747 + +0.3 65,747 + -0.5 65,747 + -1.0 65,785 - MMJJ_RS00340 0.13 -0.9 65,912 + MMJJ_RS00340 0.34 -1.8 65,959 + MMJJ_RS00340 0.42 +0.7 65,959 + MMJJ_RS00340 0.42 +2.7 65,962 + MMJJ_RS00340 0.42 -0.4 66,141 - MMJJ_RS00340 0.72 +3.1 66,142 + MMJJ_RS00340 0.72 -0.8 66,156 - MMJJ_RS00340 0.75 +0.6 66,212 + MMJJ_RS00340 0.84 +0.5 66,288 - +1.1 66,346 - -2.5 66,346 - +0.2 66,347 + -3.1 66,350 - -4.0 66,351 + -2.6 66,377 - -2.9 66,377 - -2.8 66,378 + -1.9 66,462 - MMJJ_RS00345 0.18 -2.0 66,463 + MMJJ_RS00345 0.18 -1.0 66,580 + MMJJ_RS00345 0.33 -2.3 66,710 - MMJJ_RS00345 0.49 -1.0 66,717 - MMJJ_RS00345 0.49 -2.4 66,718 + MMJJ_RS00345 0.50 -3.1 66,719 - MMJJ_RS00345 0.50 -1.2 66,719 - MMJJ_RS00345 0.50 -1.4 66,719 - MMJJ_RS00345 0.50 -2.6 66,720 + MMJJ_RS00345 0.50 -0.5 66,720 + MMJJ_RS00345 0.50 -0.7 66,720 + MMJJ_RS00345 0.50 -0.7 66,720 + MMJJ_RS00345 0.50 -2.3 66,723 - MMJJ_RS00345 0.50 -1.9 66,723 - MMJJ_RS00345 0.50 -2.3 66,723 - MMJJ_RS00345 0.50 -3.0 66,724 + MMJJ_RS00345 0.50 -0.3 66,818 - MMJJ_RS00345 0.62 -2.1 66,818 - MMJJ_RS00345 0.62 -3.0 66,827 - MMJJ_RS00345 0.63 -1.7 66,900 - MMJJ_RS00345 0.72 -3.2 66,904 + MMJJ_RS00345 0.72 -3.3 66,904 + MMJJ_RS00345 0.72 -1.8 66,950 - MMJJ_RS00345 0.78 -3.4 66,951 + MMJJ_RS00345 0.78 -2.3 66,954 - MMJJ_RS00345 0.79 -2.6 66,961 + MMJJ_RS00345 0.79 -3.4 67,041 - MMJJ_RS00345 0.89 +0.4 67,041 - MMJJ_RS00345 0.89 -3.7 67,041 - MMJJ_RS00345 0.89 -3.3 67,041 - MMJJ_RS00345 0.89 -2.6 67,042 + MMJJ_RS00345 0.89 -3.7 67,047 - -3.2 67,047 - -2.5 67,048 + +6.5 67,110 + -1.2 67,171 + -2.3 67,199 - -2.3 67,199 - -0.5 67,200 + -1.8 67,200 + -1.9 67,221 + MMJJ_RS00350 0.13 -1.4 67,234 - MMJJ_RS00350 0.15 -0.8 67,235 + MMJJ_RS00350 0.15 -2.4 67,245 + MMJJ_RS00350 0.17 -0.8 67,256 - MMJJ_RS00350 0.18 -2.1 67,256 - MMJJ_RS00350 0.18 -1.3 67,257 + MMJJ_RS00350 0.19 -0.9 67,257 + MMJJ_RS00350 0.19 -0.5 67,257 + MMJJ_RS00350 0.19 -2.1 67,276 + MMJJ_RS00350 0.22 +0.2 67,296 + MMJJ_RS00350 0.25 -1.0 67,357 - MMJJ_RS00350 0.34 -2.2 67,363 - MMJJ_RS00350 0.35 -0.3 67,363 - MMJJ_RS00350 0.35 +0.1 67,363 - MMJJ_RS00350 0.35 -0.4 67,364 + MMJJ_RS00350 0.35 -1.0 67,364 + MMJJ_RS00350 0.35 -2.4 67,364 + MMJJ_RS00350 0.35 -2.3 67,364 + MMJJ_RS00350 0.35 -1.3 67,371 + MMJJ_RS00350 0.36 -0.6 67,373 - MMJJ_RS00350 0.36 -0.5 67,373 - MMJJ_RS00350 0.36 -1.5 67,373 - MMJJ_RS00350 0.36 -2.1 67,374 + MMJJ_RS00350 0.37 +1.2 67,374 + MMJJ_RS00350 0.37 -1.7 67,394 + MMJJ_RS00350 0.40 -2.3 67,397 - MMJJ_RS00350 0.40 +1.6 67,397 - MMJJ_RS00350 0.40 -0.5 67,397 - MMJJ_RS00350 0.40 -0.9 67,397 - MMJJ_RS00350 0.40 -1.0 67,398 + MMJJ_RS00350 0.40 -0.5 67,398 + MMJJ_RS00350 0.40 +0.1 67,403 - MMJJ_RS00350 0.41 -0.2 67,404 + MMJJ_RS00350 0.41 -0.4 67,404 + -0.8 67,404 + MMJJ_RS00350 0.41 -0.4 67,408 - MMJJ_RS00350 0.42 -1.0 67,408 - MMJJ_RS00350 0.42 -0.7 67,409 + MMJJ_RS00350 0.42 -0.8 67,409 + MMJJ_RS00350 0.42 -2.4 67,433 - MMJJ_RS00350 0.46 -0.7 67,437 + MMJJ_RS00350 0.46 -2.3 67,439 - MMJJ_RS00350 0.47 -0.4 67,439 - MMJJ_RS00350 0.47 -2.6 67,439 - MMJJ_RS00350 0.47 -1.0 67,439 - MMJJ_RS00350 0.47 -3.1 67,439 - MMJJ_RS00350 0.47 -2.4 67,440 + MMJJ_RS00350 0.47 -1.2 67,451 + MMJJ_RS00350 0.48 -0.3 67,451 + MMJJ_RS00350 0.48 -1.1 67,451 + MMJJ_RS00350 0.48 -1.6 67,453 - +0.8 67,457 + MMJJ_RS00350 0.49 -0.3 67,474 - MMJJ_RS00350 0.52 -0.9 67,474 - MMJJ_RS00350 0.52 -0.1 67,555 - MMJJ_RS00350 0.64 -0.5 67,556 + MMJJ_RS00350 0.65 -1.9 67,581 - MMJJ_RS00350 0.68 +0.8 67,581 - MMJJ_RS00350 0.68 -0.6 67,581 - MMJJ_RS00350 0.68 -0.4 67,581 - MMJJ_RS00350 0.68 -0.7 67,620 - MMJJ_RS00350 0.74 -1.0 67,670 + MMJJ_RS00350 0.82 +0.0 67,670 + MMJJ_RS00350 0.82 -0.3 67,670 + MMJJ_RS00350 0.82 +0.2 67,671 - MMJJ_RS00350 0.82 -0.2 67,671 - MMJJ_RS00350 0.82 -0.5 67,672 + MMJJ_RS00350 0.82 -1.0 67,673 - MMJJ_RS00350 0.82 +0.7 67,703 + MMJJ_RS00350 0.87 -1.1 67,703 + MMJJ_RS00350 0.87 -0.8 67,768 - -0.7 67,771 - -0.9 67,801 - -1.3 67,801 - +1.0 67,801 - -1.6 67,802 + +0.5 67,803 - -1.0 67,803 - -0.4 67,803 - -0.2 67,804 + -0.1 67,804 + -0.3 67,804 + -1.9 67,804 + -0.4 67,804 + +0.2 67,808 - +0.4 67,808 - +1.3 67,808 - +1.4 67,809 + +0.9 67,827 - +1.0 67,827 - +0.4 67,829 - +0.2 67,829 - +0.3 67,829 - -0.1 67,829 - +3.1 67,830 + +0.1 67,830 + +0.6 67,830 + -0.4 67,830 + -0.3 67,830 + +1.3 67,830 + +0.4 67,831 - +1.3 67,831 - -1.0 67,831 - +0.8 67,831 - +0.2 67,832 + -3.0 67,832 + +0.9 67,832 + +0.8 67,834 - -0.5 67,834 - -0.3 67,834 - -0.8 67,834 - -0.1 67,834 - +0.5 67,834 - +1.5 67,834 - +1.0 67,834 - -0.2 67,834 - +0.1 67,835 + -1.1 67,835 + +0.5 67,835 + -0.8 67,835 + -0.2 67,835 + +0.1 67,835 + +2.3 67,835 + +0.1 67,835 + -0.9 67,835 + +0.6 67,835 + -0.2 67,835 + -1.2 67,835 + -0.5 67,835 + +0.2 67,836 - +0.5 67,836 - -0.3 67,837 + -0.4 67,837 + +0.5 67,837 + -2.0 67,837 + -0.1 67,837 + +0.2 67,849 - -0.2 67,849 - +0.8 67,850 + -0.0 67,853 - +2.4 67,853 - +1.5 67,853 - -0.4 67,853 - -0.2 67,854 + +0.9 67,854 + +0.9 67,854 + +1.0 67,854 + -0.2 67,857 - -0.2 67,857 - +0.6 67,857 - -1.1 67,858 + -0.7 67,858 + -0.3 67,858 + -0.9 67,858 + -0.5 67,858 + +0.7 67,863 - -0.8 67,863 - +1.4 67,881 - +0.5 67,881 - -0.8 67,882 + +0.3 67,895 - -0.1 67,902 + -1.1 67,960 - MMJJ_RS00355 0.18 -2.9 68,012 - MMJJ_RS00355 0.34 -0.8 68,014 - MMJJ_RS00355 0.34 -1.2 68,014 - MMJJ_RS00355 0.34 +0.2 68,015 + MMJJ_RS00355 0.35 -2.2 68,108 + MMJJ_RS00355 0.62 -1.0
Or see this region's nucleotide sequence