Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1214 and recA are separated by 330 nucleotides recA and Shew_1216 are separated by 79 nucleotides Shew_1216 and Shew_1217 are separated by 244 nucleotides
Shew_1214: Shew_1214 - DNA mismatch repair protein (RefSeq), at 1,408,043 to 1,410,610
_1214
Shew_1215: recA - recombinase A (RefSeq), at 1,410,941 to 1,412,002
recA
Shew_1216: Shew_1216 - regulatory protein RecX (RefSeq), at 1,412,082 to 1,412,534
_1216
Shew_1217: Shew_1217 - alanyl-tRNA synthetase (RefSeq), at 1,412,779 to 1,415,403
_1217
Position (kb)
1410
1411
1412
1413 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1410.071 kb on - strand, within Shew_1214 at 1410.236 kb on + strand, within Shew_1214 at 1410.342 kb on + strand, within Shew_1214 at 1410.620 kb on + strand at 1410.783 kb on - strand at 1410.856 kb on + strand at 1410.856 kb on + strand at 1410.856 kb on + strand at 1410.856 kb on + strand at 1410.864 kb on - strand at 1410.864 kb on - strand at 1410.900 kb on + strand at 1411.185 kb on + strand, within recA at 1411.495 kb on + strand, within recA at 1411.549 kb on - strand, within recA at 1411.575 kb on + strand, within recA at 1412.153 kb on + strand, within Shew_1216 at 1412.291 kb on + strand, within Shew_1216 at 1412.294 kb on - strand, within Shew_1216 at 1412.304 kb on - strand, within Shew_1216 at 1412.455 kb on - strand, within Shew_1216
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 1,410,071 - Shew_1214 0.79 +0.4 1,410,236 + Shew_1214 0.85 +0.4 1,410,342 + Shew_1214 0.90 +0.4 1,410,620 + +1.0 1,410,783 - -0.9 1,410,856 + -1.3 1,410,856 + -2.4 1,410,856 + -4.5 1,410,856 + -4.3 1,410,864 - -3.7 1,410,864 - -4.2 1,410,900 + -2.4 1,411,185 + recA Shew_1215 0.23 -3.3 1,411,495 + recA Shew_1215 0.52 -3.9 1,411,549 - recA Shew_1215 0.57 -2.3 1,411,575 + recA Shew_1215 0.60 -3.0 1,412,153 + Shew_1216 0.16 +0.1 1,412,291 + Shew_1216 0.46 +0.1 1,412,294 - Shew_1216 0.47 -0.4 1,412,304 - Shew_1216 0.49 -0.1 1,412,455 - Shew_1216 0.82 -0.4
Or see this region's nucleotide sequence