Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0623 and Shew_0624 are separated by 70 nucleotides Shew_0624 and Shew_0625 are separated by 90 nucleotides Shew_0625 and Shew_0626 are separated by 13 nucleotides Shew_0626 and Shew_0627 are separated by 11 nucleotides
Shew_0623: Shew_0623 - phage shock protein A, PspA (RefSeq), at 707,888 to 708,574
_0623
Shew_0624: Shew_0624 - hypothetical protein (RefSeq), at 708,645 to 709,058
_0624
Shew_0625: Shew_0625 - spermidine synthase (RefSeq), at 709,149 to 710,876
_0625
Shew_0626: Shew_0626 - hypothetical protein (RefSeq), at 710,890 to 711,789
_0626
Shew_0627: Shew_0627 - hypothetical protein (RefSeq), at 711,801 to 712,757
_0627
Position (kb) 709
710
711 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 708.203 kb on - strand, within Shew_0623 at 708.685 kb on - strand at 709.233 kb on + strand at 709.289 kb on + strand at 709.289 kb on + strand at 709.289 kb on + strand at 709.297 kb on - strand at 709.300 kb on + strand at 709.448 kb on + strand, within Shew_0625 at 709.496 kb on - strand, within Shew_0625 at 709.607 kb on + strand, within Shew_0625 at 709.703 kb on - strand, within Shew_0625 at 709.822 kb on + strand, within Shew_0625 at 709.841 kb on + strand, within Shew_0625 at 710.222 kb on + strand, within Shew_0625 at 710.222 kb on + strand, within Shew_0625 at 710.543 kb on + strand, within Shew_0625 at 710.880 kb on + strand at 710.991 kb on + strand, within Shew_0626 at 711.036 kb on + strand, within Shew_0626 at 711.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 708,203 - Shew_0623 0.46 -4.9 708,685 - -0.3 709,233 + +0.4 709,289 + +0.6 709,289 + +0.8 709,289 + +0.1 709,297 - +0.6 709,300 + +0.4 709,448 + Shew_0625 0.17 +0.8 709,496 - Shew_0625 0.20 +0.8 709,607 + Shew_0625 0.27 +0.6 709,703 - Shew_0625 0.32 -0.0 709,822 + Shew_0625 0.39 +0.3 709,841 + Shew_0625 0.40 +0.6 710,222 + Shew_0625 0.62 +0.8 710,222 + Shew_0625 0.62 +0.9 710,543 + Shew_0625 0.81 +0.8 710,880 + +0.8 710,991 + Shew_0626 0.11 +0.6 711,036 + Shew_0626 0.16 +0.4 711,835 - +0.8
Or see this region's nucleotide sequence