Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0283 and Shew_0284 are separated by 329 nucleotides Shew_0284 and Shew_0285 are separated by 28 nucleotides
Shew_0283: Shew_0283 - Mg chelatase, subunit ChlI (RefSeq), at 300,316 to 301,836
_0283
Shew_0284: Shew_0284 - hypothetical protein (RefSeq), at 302,166 to 303,491
_0284
Shew_0285: Shew_0285 - diguanylate cyclase (RefSeq), at 303,520 to 304,401
_0285
Position (kb) 302
303
304 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 301.279 kb on - strand, within Shew_0283 at 301.347 kb on - strand, within Shew_0283 at 301.484 kb on + strand, within Shew_0283 at 301.683 kb on - strand, within Shew_0283 at 301.718 kb on + strand at 301.737 kb on + strand at 302.071 kb on + strand at 302.139 kb on - strand at 302.139 kb on - strand at 302.468 kb on + strand, within Shew_0284 at 302.481 kb on - strand, within Shew_0284 at 302.481 kb on - strand, within Shew_0284 at 302.621 kb on + strand, within Shew_0284 at 302.650 kb on - strand, within Shew_0284 at 302.698 kb on + strand, within Shew_0284 at 302.859 kb on - strand, within Shew_0284 at 303.036 kb on + strand, within Shew_0284 at 303.046 kb on + strand, within Shew_0284 at 303.046 kb on + strand, within Shew_0284 at 303.046 kb on + strand, within Shew_0284 at 303.257 kb on - strand, within Shew_0284 at 303.279 kb on + strand, within Shew_0284 at 303.388 kb on + strand at 303.542 kb on - strand at 303.561 kb on + strand at 303.584 kb on - strand at 303.584 kb on + strand at 303.784 kb on - strand, within Shew_0285 at 304.174 kb on + strand, within Shew_0285
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 301,279 - Shew_0283 0.63 +0.3 301,347 - Shew_0283 0.68 -0.1 301,484 + Shew_0283 0.77 +0.0 301,683 - Shew_0283 0.90 +0.1 301,718 + +1.0 301,737 + +0.1 302,071 + +2.8 302,139 - -2.1 302,139 - -2.9 302,468 + Shew_0284 0.23 -1.0 302,481 - Shew_0284 0.24 -2.4 302,481 - Shew_0284 0.24 -4.3 302,621 + Shew_0284 0.34 -3.1 302,650 - Shew_0284 0.37 -2.2 302,698 + Shew_0284 0.40 -2.1 302,859 - Shew_0284 0.52 -2.1 303,036 + Shew_0284 0.66 -2.5 303,046 + Shew_0284 0.66 -2.2 303,046 + Shew_0284 0.66 -2.7 303,046 + Shew_0284 0.66 -2.2 303,257 - Shew_0284 0.82 -2.8 303,279 + Shew_0284 0.84 -2.8 303,388 + -1.8 303,542 - +0.2 303,561 + -0.1 303,584 - -0.4 303,584 + +0.1 303,784 - Shew_0285 0.30 -0.3 304,174 + Shew_0285 0.74 -0.3
Or see this region's nucleotide sequence