Strain Fitness in Dinoroseobacter shibae DFL-12 around Dshi_0253

Experiment: m.b. Benzethonium chloride 0.008 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntDshi_0251 and Dshi_0252 are separated by 77 nucleotidesDshi_0252 and Dshi_0253 are separated by 55 nucleotidesDshi_0253 and Dshi_0254 are separated by 30 nucleotidesDshi_0254 and Dshi_0255 are separated by 156 nucleotides Dshi_0251: Dshi_0251 - efflux transporter, RND family, MFP subunit (RefSeq), at 249,241 to 250,392 _0251 Dshi_0252: Dshi_0252 - transcriptional regulator, TetR family (RefSeq), at 250,470 to 251,144 _0252 Dshi_0253: Dshi_0253 - AMP-dependent synthetase and ligase (RefSeq), at 251,200 to 252,759 _0253 Dshi_0254: Dshi_0254 - ribonuclease T2 (RefSeq), at 252,790 to 253,431 _0254 Dshi_0255: Dshi_0255 - protein of unknown function DUF1013 (RefSeq), at 253,588 to 254,340 _0255 Position (kb) 251 252 253Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 250.245 kb on - strand, within Dshi_0251at 250.303 kb on + strandat 250.303 kb on + strandat 250.303 kb on + strandat 250.383 kb on + strandat 250.555 kb on + strand, within Dshi_0252at 250.612 kb on - strand, within Dshi_0252at 250.675 kb on + strand, within Dshi_0252at 250.728 kb on - strand, within Dshi_0252at 250.801 kb on + strand, within Dshi_0252at 250.823 kb on - strand, within Dshi_0252at 251.019 kb on + strand, within Dshi_0252at 251.146 kb on - strandat 251.185 kb on - strandat 251.227 kb on + strandat 251.244 kb on - strandat 251.273 kb on - strandat 251.351 kb on - strandat 251.375 kb on + strand, within Dshi_0253at 251.422 kb on + strand, within Dshi_0253at 251.497 kb on - strand, within Dshi_0253at 251.601 kb on + strand, within Dshi_0253at 251.664 kb on + strand, within Dshi_0253at 251.751 kb on + strand, within Dshi_0253at 251.751 kb on + strand, within Dshi_0253at 251.782 kb on - strand, within Dshi_0253at 251.832 kb on + strand, within Dshi_0253at 251.832 kb on + strand, within Dshi_0253at 251.840 kb on - strand, within Dshi_0253at 251.840 kb on - strand, within Dshi_0253at 251.899 kb on - strand, within Dshi_0253at 251.901 kb on + strand, within Dshi_0253at 251.917 kb on + strand, within Dshi_0253at 251.925 kb on - strand, within Dshi_0253at 251.969 kb on - strand, within Dshi_0253at 252.026 kb on + strand, within Dshi_0253at 252.051 kb on + strand, within Dshi_0253at 252.146 kb on + strand, within Dshi_0253at 252.146 kb on + strand, within Dshi_0253at 252.208 kb on + strand, within Dshi_0253at 252.264 kb on - strand, within Dshi_0253at 252.425 kb on - strand, within Dshi_0253at 252.444 kb on + strand, within Dshi_0253at 252.570 kb on - strand, within Dshi_0253at 252.617 kb on + strandat 252.668 kb on + strandat 252.737 kb on - strandat 252.748 kb on + strandat 252.793 kb on + strandat 252.796 kb on - strandat 252.869 kb on - strand, within Dshi_0254at 252.878 kb on + strand, within Dshi_0254at 252.892 kb on + strand, within Dshi_0254at 252.939 kb on - strand, within Dshi_0254at 253.305 kb on + strand, within Dshi_0254at 253.370 kb on - strandat 253.388 kb on - strandat 253.416 kb on - strandat 253.419 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction m.b. Benzethonium chloride 0.008 mM
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250,245 - Dshi_0251 0.87 +0.3
250,303 + -0.4
250,303 + -1.3
250,303 + -3.8
250,383 + +0.2
250,555 + Dshi_0252 0.13 -0.1
250,612 - Dshi_0252 0.21 +0.7
250,675 + Dshi_0252 0.30 +0.5
250,728 - Dshi_0252 0.38 +1.1
250,801 + Dshi_0252 0.49 +1.7
250,823 - Dshi_0252 0.52 +2.2
251,019 + Dshi_0252 0.81 +0.4
251,146 - +0.3
251,185 - -0.9
251,227 + +2.3
251,244 - -3.8
251,273 - +1.3
251,351 - +0.4
251,375 + Dshi_0253 0.11 -0.3
251,422 + Dshi_0253 0.14 -1.0
251,497 - Dshi_0253 0.19 -0.6
251,601 + Dshi_0253 0.26 -2.6
251,664 + Dshi_0253 0.30 -1.3
251,751 + Dshi_0253 0.35 +1.8
251,751 + Dshi_0253 0.35 -1.0
251,782 - Dshi_0253 0.37 -0.4
251,832 + Dshi_0253 0.41 -0.1
251,832 + Dshi_0253 0.41 +0.5
251,840 - Dshi_0253 0.41 -0.5
251,840 - Dshi_0253 0.41 +0.2
251,899 - Dshi_0253 0.45 -3.5
251,901 + Dshi_0253 0.45 +0.2
251,917 + Dshi_0253 0.46 -1.0
251,925 - Dshi_0253 0.46 -0.2
251,969 - Dshi_0253 0.49 +0.1
252,026 + Dshi_0253 0.53 +0.2
252,051 + Dshi_0253 0.55 -0.3
252,146 + Dshi_0253 0.61 -3.0
252,146 + Dshi_0253 0.61 +0.3
252,208 + Dshi_0253 0.65 -0.6
252,264 - Dshi_0253 0.68 -2.2
252,425 - Dshi_0253 0.79 +0.7
252,444 + Dshi_0253 0.80 +1.1
252,570 - Dshi_0253 0.88 +0.5
252,617 + -1.5
252,668 + -2.3
252,737 - -0.8
252,748 + +1.5
252,793 + -0.2
252,796 - +0.9
252,869 - Dshi_0254 0.12 +0.7
252,878 + Dshi_0254 0.14 +0.6
252,892 + Dshi_0254 0.16 -0.8
252,939 - Dshi_0254 0.23 -1.6
253,305 + Dshi_0254 0.80 -0.8
253,370 - +1.6
253,388 - +1.8
253,416 - -0.3
253,419 - -0.0

Or see this region's nucleotide sequence