Experiment: BHIS with Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3898 and BT3899 are separated by 17 nucleotides BT3899 and BT3900 overlap by 2 nucleotides BT3900 and BT3901 are separated by 115 nucleotides BT3901 and BT3902 are separated by 121 nucleotides
BT3898: BT3898 - TonB (NCBI ptt file), at 5,069,671 to 5,071,500
BT3898
BT3899: BT3899 - transcriptional regulator (NCBI ptt file), at 5,071,518 to 5,071,883
BT3899
BT3900: BT3900 - hypothetical protein (NCBI ptt file), at 5,071,882 to 5,072,115
BT3900
BT3901: BT3901 - hypothetical protein (NCBI ptt file), at 5,072,231 to 5,072,887
BT3901
BT3902: BT3902 - conserved hypothetical protein (NCBI ptt file), at 5,073,009 to 5,074,268
BT3902
Position (kb)
5071
5072
5073 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5070.931 kb on - strand, within BT3898 at 5070.931 kb on - strand, within BT3898 at 5070.994 kb on + strand, within BT3898 at 5071.034 kb on - strand, within BT3898 at 5071.034 kb on - strand, within BT3898 at 5071.034 kb on - strand, within BT3898 at 5071.078 kb on + strand, within BT3898 at 5071.081 kb on + strand, within BT3898 at 5071.081 kb on + strand, within BT3898 at 5071.082 kb on - strand, within BT3898 at 5071.087 kb on + strand, within BT3898 at 5071.087 kb on + strand, within BT3898 at 5071.087 kb on + strand, within BT3898 at 5071.088 kb on - strand, within BT3898 at 5071.088 kb on - strand, within BT3898 at 5071.135 kb on + strand, within BT3898 at 5071.141 kb on - strand, within BT3898 at 5071.178 kb on - strand, within BT3898 at 5071.193 kb on + strand, within BT3898 at 5071.197 kb on + strand, within BT3898 at 5071.220 kb on - strand, within BT3898 at 5071.382 kb on - strand at 5071.382 kb on - strand at 5071.445 kb on + strand at 5071.501 kb on - strand at 5071.501 kb on - strand at 5071.540 kb on - strand at 5071.540 kb on - strand at 5071.571 kb on + strand, within BT3899 at 5071.572 kb on - strand, within BT3899 at 5071.575 kb on + strand, within BT3899 at 5071.614 kb on - strand, within BT3899 at 5071.683 kb on - strand, within BT3899 at 5071.683 kb on - strand, within BT3899 at 5071.693 kb on + strand, within BT3899 at 5071.693 kb on + strand, within BT3899 at 5071.694 kb on - strand, within BT3899 at 5071.703 kb on + strand, within BT3899 at 5071.710 kb on + strand, within BT3899 at 5071.711 kb on - strand, within BT3899 at 5071.729 kb on + strand, within BT3899 at 5071.772 kb on + strand, within BT3899 at 5071.772 kb on + strand, within BT3899 at 5071.774 kb on + strand, within BT3899 at 5071.775 kb on - strand, within BT3899 at 5071.816 kb on - strand, within BT3899 at 5071.843 kb on + strand, within BT3899 at 5071.955 kb on + strand, within BT3900 at 5071.955 kb on + strand, within BT3900 at 5071.960 kb on - strand, within BT3900 at 5072.046 kb on + strand, within BT3900 at 5072.079 kb on - strand, within BT3900 at 5072.097 kb on - strand at 5072.152 kb on + strand at 5072.158 kb on - strand at 5072.225 kb on + strand at 5072.226 kb on - strand at 5072.228 kb on + strand at 5072.229 kb on - strand at 5072.229 kb on - strand at 5072.321 kb on - strand, within BT3901 at 5072.353 kb on + strand, within BT3901 at 5072.354 kb on - strand, within BT3901 at 5072.358 kb on + strand, within BT3901 at 5072.363 kb on - strand, within BT3901 at 5072.409 kb on + strand, within BT3901 at 5072.409 kb on + strand, within BT3901 at 5072.410 kb on - strand, within BT3901 at 5072.452 kb on + strand, within BT3901 at 5072.452 kb on + strand, within BT3901 at 5072.452 kb on + strand, within BT3901 at 5072.453 kb on - strand, within BT3901 at 5072.453 kb on - strand, within BT3901 at 5072.461 kb on + strand, within BT3901 at 5072.509 kb on + strand, within BT3901 at 5072.509 kb on + strand, within BT3901 at 5072.510 kb on - strand, within BT3901 at 5072.518 kb on + strand, within BT3901 at 5072.587 kb on - strand, within BT3901 at 5072.592 kb on + strand, within BT3901 at 5072.633 kb on + strand, within BT3901 at 5072.770 kb on + strand, within BT3901 at 5072.848 kb on + strand at 5072.860 kb on - strand at 5072.863 kb on - strand at 5072.943 kb on - strand at 5072.958 kb on - strand at 5072.959 kb on + strand at 5072.960 kb on - strand at 5072.960 kb on - strand at 5072.983 kb on - strand at 5072.983 kb on - strand at 5073.002 kb on - strand at 5073.056 kb on + strand at 5073.057 kb on - strand at 5073.057 kb on - strand at 5073.058 kb on + strand at 5073.067 kb on - strand at 5073.104 kb on - strand at 5073.104 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Vancomycin 0.016 mg/ml remove 5,070,931 - BT3898 0.69 +0.3 5,070,931 - BT3898 0.69 -0.4 5,070,994 + BT3898 0.72 -0.5 5,071,034 - BT3898 0.74 +0.3 5,071,034 - BT3898 0.74 -0.5 5,071,034 - BT3898 0.74 +1.9 5,071,078 + BT3898 0.77 -0.4 5,071,081 + BT3898 0.77 -2.7 5,071,081 + BT3898 0.77 -0.6 5,071,082 - BT3898 0.77 +1.1 5,071,087 + BT3898 0.77 -0.1 5,071,087 + BT3898 0.77 +0.2 5,071,087 + BT3898 0.77 -1.0 5,071,088 - BT3898 0.77 +1.5 5,071,088 - BT3898 0.77 +1.1 5,071,135 + BT3898 0.80 +0.1 5,071,141 - BT3898 0.80 -1.1 5,071,178 - BT3898 0.82 -1.9 5,071,193 + BT3898 0.83 -0.6 5,071,197 + BT3898 0.83 -1.5 5,071,220 - BT3898 0.85 +0.2 5,071,382 - +1.1 5,071,382 - -1.4 5,071,445 + +0.7 5,071,501 - -2.4 5,071,501 - -0.3 5,071,540 - -0.8 5,071,540 - -0.3 5,071,571 + BT3899 0.14 -0.3 5,071,572 - BT3899 0.15 +0.5 5,071,575 + BT3899 0.16 -0.8 5,071,614 - BT3899 0.26 -0.4 5,071,683 - BT3899 0.45 +2.1 5,071,683 - BT3899 0.45 +1.9 5,071,693 + BT3899 0.48 +1.1 5,071,693 + BT3899 0.48 +1.8 5,071,694 - BT3899 0.48 +0.7 5,071,703 + BT3899 0.51 +0.0 5,071,710 + BT3899 0.52 +0.8 5,071,711 - BT3899 0.53 +0.9 5,071,729 + BT3899 0.58 -2.0 5,071,772 + BT3899 0.69 +0.9 5,071,772 + BT3899 0.69 +0.8 5,071,774 + BT3899 0.70 -0.5 5,071,775 - BT3899 0.70 +1.4 5,071,816 - BT3899 0.81 +0.1 5,071,843 + BT3899 0.89 +0.8 5,071,955 + BT3900 0.31 +0.7 5,071,955 + BT3900 0.31 -2.1 5,071,960 - BT3900 0.33 -2.4 5,072,046 + BT3900 0.70 +0.1 5,072,079 - BT3900 0.84 +2.0 5,072,097 - -0.6 5,072,152 + +0.3 5,072,158 - +0.2 5,072,225 + +1.0 5,072,226 - -0.1 5,072,228 + -0.2 5,072,229 - -1.0 5,072,229 - +0.3 5,072,321 - BT3901 0.14 +0.5 5,072,353 + BT3901 0.19 +0.2 5,072,354 - BT3901 0.19 +3.1 5,072,358 + BT3901 0.19 +0.2 5,072,363 - BT3901 0.20 +1.2 5,072,409 + BT3901 0.27 -0.9 5,072,409 + BT3901 0.27 -1.6 5,072,410 - BT3901 0.27 +0.3 5,072,452 + BT3901 0.34 -1.0 5,072,452 + BT3901 0.34 -2.0 5,072,452 + BT3901 0.34 -0.9 5,072,453 - BT3901 0.34 +0.3 5,072,453 - BT3901 0.34 +1.1 5,072,461 + BT3901 0.35 -3.2 5,072,509 + BT3901 0.42 -0.5 5,072,509 + BT3901 0.42 -0.9 5,072,510 - BT3901 0.42 +1.4 5,072,518 + BT3901 0.44 -0.6 5,072,587 - BT3901 0.54 +1.1 5,072,592 + BT3901 0.55 -1.4 5,072,633 + BT3901 0.61 -2.5 5,072,770 + BT3901 0.82 -2.5 5,072,848 + +2.7 5,072,860 - +0.4 5,072,863 - -0.5 5,072,943 - +0.3 5,072,958 - -2.3 5,072,959 + +0.4 5,072,960 - -0.1 5,072,960 - +0.9 5,072,983 - -0.7 5,072,983 - -0.7 5,073,002 - +1.3 5,073,056 + -0.8 5,073,057 - -0.6 5,073,057 - -0.9 5,073,058 + -1.8 5,073,067 - +0.1 5,073,104 - -1.2 5,073,104 - +0.1
Or see this region's nucleotide sequence