Experiment: BHIS with Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2664 and BT2665 are separated by 1 nucleotides BT2665 and BT2666 are separated by 26 nucleotides BT2666 and BT2667 are separated by 15 nucleotides BT2667 and BT2668 are separated by 29 nucleotides
BT2664: BT2664 - putative phosphate ABC transporter, phosphate-binding component (NCBI ptt file), at 3,320,412 to 3,321,356
BT2664
BT2665: BT2665 - TonB (NCBI ptt file), at 3,321,358 to 3,322,170
BT2665
BT2666: BT2666 - conserved hypothetical protein (NCBI ptt file), at 3,322,197 to 3,322,850
BT2666
BT2667: BT2667 - conserved hypothetical protein (NCBI ptt file), at 3,322,866 to 3,323,471
BT2667
BT2668: BT2668 - MotA/TolQ/ExbB proton channel (NCBI ptt file), at 3,323,501 to 3,324,298
BT2668
Position (kb)
3322
3323 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3321.218 kb on + strand, within BT2664 at 3321.219 kb on - strand, within BT2664 at 3321.265 kb on + strand at 3321.266 kb on - strand at 3321.330 kb on + strand at 3321.359 kb on + strand at 3321.359 kb on + strand at 3321.360 kb on - strand at 3321.369 kb on + strand at 3321.442 kb on - strand, within BT2665 at 3321.442 kb on - strand, within BT2665 at 3321.442 kb on - strand, within BT2665 at 3321.468 kb on + strand, within BT2665 at 3321.472 kb on + strand, within BT2665 at 3321.473 kb on - strand, within BT2665 at 3321.497 kb on - strand, within BT2665 at 3321.497 kb on - strand, within BT2665 at 3321.513 kb on + strand, within BT2665 at 3321.513 kb on + strand, within BT2665 at 3321.516 kb on + strand, within BT2665 at 3321.529 kb on + strand, within BT2665 at 3321.587 kb on + strand, within BT2665 at 3321.608 kb on + strand, within BT2665 at 3321.660 kb on + strand, within BT2665 at 3321.661 kb on - strand, within BT2665 at 3321.742 kb on - strand, within BT2665 at 3321.777 kb on + strand, within BT2665 at 3321.858 kb on + strand, within BT2665 at 3321.971 kb on - strand, within BT2665 at 3322.009 kb on - strand, within BT2665 at 3322.059 kb on + strand, within BT2665 at 3322.062 kb on + strand, within BT2665 at 3322.062 kb on + strand, within BT2665 at 3322.062 kb on + strand, within BT2665 at 3322.063 kb on - strand, within BT2665 at 3322.092 kb on + strand at 3322.193 kb on + strand at 3322.193 kb on + strand at 3322.194 kb on - strand at 3322.288 kb on + strand, within BT2666 at 3322.292 kb on + strand, within BT2666 at 3322.293 kb on - strand, within BT2666 at 3322.296 kb on + strand, within BT2666 at 3322.297 kb on - strand, within BT2666 at 3322.298 kb on + strand, within BT2666 at 3322.298 kb on + strand, within BT2666 at 3322.301 kb on + strand, within BT2666 at 3322.301 kb on + strand, within BT2666 at 3322.301 kb on + strand, within BT2666 at 3322.301 kb on + strand, within BT2666 at 3322.302 kb on - strand, within BT2666 at 3322.305 kb on + strand, within BT2666 at 3322.506 kb on + strand, within BT2666 at 3322.535 kb on + strand, within BT2666 at 3322.535 kb on + strand, within BT2666 at 3322.555 kb on + strand, within BT2666 at 3322.555 kb on + strand, within BT2666 at 3322.564 kb on + strand, within BT2666 at 3322.564 kb on + strand, within BT2666 at 3322.569 kb on - strand, within BT2666 at 3322.582 kb on + strand, within BT2666 at 3322.583 kb on - strand, within BT2666 at 3322.585 kb on + strand, within BT2666 at 3322.588 kb on + strand, within BT2666 at 3322.588 kb on + strand, within BT2666 at 3322.589 kb on - strand, within BT2666 at 3322.614 kb on + strand, within BT2666 at 3322.615 kb on - strand, within BT2666 at 3322.664 kb on + strand, within BT2666 at 3322.722 kb on - strand, within BT2666 at 3322.739 kb on + strand, within BT2666 at 3322.757 kb on + strand, within BT2666 at 3322.941 kb on + strand, within BT2667 at 3322.950 kb on + strand, within BT2667 at 3322.982 kb on + strand, within BT2667 at 3322.982 kb on + strand, within BT2667 at 3322.982 kb on + strand, within BT2667 at 3322.983 kb on - strand, within BT2667 at 3323.021 kb on + strand, within BT2667 at 3323.051 kb on + strand, within BT2667 at 3323.054 kb on + strand, within BT2667 at 3323.201 kb on - strand, within BT2667 at 3323.262 kb on + strand, within BT2667 at 3323.295 kb on + strand, within BT2667 at 3323.409 kb on + strand, within BT2667 at 3323.409 kb on + strand, within BT2667 at 3323.410 kb on - strand, within BT2667 at 3323.429 kb on + strand at 3323.429 kb on + strand at 3323.429 kb on + strand at 3323.433 kb on + strand at 3323.434 kb on - strand at 3323.475 kb on + strand at 3323.475 kb on + strand at 3323.502 kb on + strand at 3323.657 kb on - strand, within BT2668 at 3323.755 kb on + strand, within BT2668 at 3323.756 kb on - strand, within BT2668 at 3323.759 kb on + strand, within BT2668 at 3323.765 kb on - strand, within BT2668 at 3323.765 kb on - strand, within BT2668 at 3323.767 kb on + strand, within BT2668 at 3323.768 kb on - strand, within BT2668 at 3323.803 kb on + strand, within BT2668
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Vancomycin 0.016 mg/ml remove 3,321,218 + BT2664 0.85 -0.9 3,321,219 - BT2664 0.85 -0.8 3,321,265 + -0.8 3,321,266 - +0.9 3,321,330 + +2.2 3,321,359 + +0.3 3,321,359 + +0.0 3,321,360 - +0.8 3,321,369 + -2.3 3,321,442 - BT2665 0.10 +2.5 3,321,442 - BT2665 0.10 +4.3 3,321,442 - BT2665 0.10 +2.3 3,321,468 + BT2665 0.14 +3.2 3,321,472 + BT2665 0.14 -0.4 3,321,473 - BT2665 0.14 +2.1 3,321,497 - BT2665 0.17 +0.5 3,321,497 - BT2665 0.17 +0.7 3,321,513 + BT2665 0.19 +0.6 3,321,513 + BT2665 0.19 -0.6 3,321,516 + BT2665 0.19 +0.2 3,321,529 + BT2665 0.21 -1.3 3,321,587 + BT2665 0.28 -0.8 3,321,608 + BT2665 0.31 +0.6 3,321,660 + BT2665 0.37 +0.5 3,321,661 - BT2665 0.37 +1.5 3,321,742 - BT2665 0.47 +2.8 3,321,777 + BT2665 0.52 -2.2 3,321,858 + BT2665 0.62 -1.1 3,321,971 - BT2665 0.75 +3.6 3,322,009 - BT2665 0.80 +1.3 3,322,059 + BT2665 0.86 +0.2 3,322,062 + BT2665 0.87 +1.2 3,322,062 + BT2665 0.87 -0.7 3,322,062 + BT2665 0.87 -1.8 3,322,063 - BT2665 0.87 +1.7 3,322,092 + -1.2 3,322,193 + -0.1 3,322,193 + -0.6 3,322,194 - +2.9 3,322,288 + BT2666 0.14 +1.7 3,322,292 + BT2666 0.15 +0.9 3,322,293 - BT2666 0.15 +2.5 3,322,296 + BT2666 0.15 +0.1 3,322,297 - BT2666 0.15 +0.2 3,322,298 + BT2666 0.15 +0.6 3,322,298 + BT2666 0.15 +0.7 3,322,301 + BT2666 0.16 +0.2 3,322,301 + BT2666 0.16 -0.8 3,322,301 + BT2666 0.16 +0.0 3,322,301 + BT2666 0.16 +0.0 3,322,302 - BT2666 0.16 +4.0 3,322,305 + BT2666 0.17 -1.0 3,322,506 + BT2666 0.47 -1.9 3,322,535 + BT2666 0.52 +1.2 3,322,535 + BT2666 0.52 +2.4 3,322,555 + BT2666 0.55 -0.1 3,322,555 + BT2666 0.55 -2.8 3,322,564 + BT2666 0.56 +0.3 3,322,564 + BT2666 0.56 -0.8 3,322,569 - BT2666 0.57 +2.4 3,322,582 + BT2666 0.59 +0.2 3,322,583 - BT2666 0.59 +5.7 3,322,585 + BT2666 0.59 -0.1 3,322,588 + BT2666 0.60 -0.3 3,322,588 + BT2666 0.60 -0.6 3,322,589 - BT2666 0.60 +3.5 3,322,614 + BT2666 0.64 +0.3 3,322,615 - BT2666 0.64 +2.4 3,322,664 + BT2666 0.71 -1.8 3,322,722 - BT2666 0.80 +3.8 3,322,739 + BT2666 0.83 -0.5 3,322,757 + BT2666 0.86 -1.4 3,322,941 + BT2667 0.12 +0.0 3,322,950 + BT2667 0.14 -0.2 3,322,982 + BT2667 0.19 -0.1 3,322,982 + BT2667 0.19 -0.8 3,322,982 + BT2667 0.19 +2.0 3,322,983 - BT2667 0.19 +4.3 3,323,021 + BT2667 0.26 -0.9 3,323,051 + BT2667 0.31 +0.2 3,323,054 + BT2667 0.31 +0.3 3,323,201 - BT2667 0.55 +1.8 3,323,262 + BT2667 0.65 +0.8 3,323,295 + BT2667 0.71 +0.2 3,323,409 + BT2667 0.90 -1.1 3,323,409 + BT2667 0.90 +0.0 3,323,410 - BT2667 0.90 +2.3 3,323,429 + +0.1 3,323,429 + -1.3 3,323,429 + -1.4 3,323,433 + -0.2 3,323,434 - +3.1 3,323,475 + -1.0 3,323,475 + +0.2 3,323,502 + +2.8 3,323,657 - BT2668 0.20 +0.1 3,323,755 + BT2668 0.32 -0.1 3,323,756 - BT2668 0.32 +1.2 3,323,759 + BT2668 0.32 -0.3 3,323,765 - BT2668 0.33 +0.2 3,323,765 - BT2668 0.33 +1.2 3,323,767 + BT2668 0.33 -0.1 3,323,768 - BT2668 0.33 -1.8 3,323,803 + BT2668 0.38 +0.5
Or see this region's nucleotide sequence