Experiment: BHIS with Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2663 and BT2664 are separated by 36 nucleotides BT2664 and BT2665 are separated by 1 nucleotides BT2665 and BT2666 are separated by 26 nucleotides
BT2663: BT2663 - tetratricopeptide repeat family protein (NCBI ptt file), at 3,318,924 to 3,320,375
BT2663
BT2664: BT2664 - putative phosphate ABC transporter, phosphate-binding component (NCBI ptt file), at 3,320,412 to 3,321,356
BT2664
BT2665: BT2665 - TonB (NCBI ptt file), at 3,321,358 to 3,322,170
BT2665
BT2666: BT2666 - conserved hypothetical protein (NCBI ptt file), at 3,322,197 to 3,322,850
BT2666
Position (kb)
3320
3321
3322 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3319.442 kb on - strand, within BT2663 at 3319.482 kb on + strand, within BT2663 at 3319.504 kb on - strand, within BT2663 at 3319.509 kb on + strand, within BT2663 at 3319.523 kb on - strand, within BT2663 at 3319.576 kb on + strand, within BT2663 at 3319.577 kb on - strand, within BT2663 at 3319.581 kb on - strand, within BT2663 at 3319.581 kb on - strand, within BT2663 at 3319.582 kb on + strand, within BT2663 at 3319.582 kb on + strand, within BT2663 at 3319.582 kb on + strand, within BT2663 at 3319.591 kb on + strand, within BT2663 at 3319.642 kb on - strand, within BT2663 at 3319.672 kb on + strand, within BT2663 at 3319.673 kb on - strand, within BT2663 at 3319.673 kb on - strand, within BT2663 at 3319.676 kb on + strand, within BT2663 at 3319.676 kb on + strand, within BT2663 at 3319.677 kb on - strand, within BT2663 at 3319.678 kb on + strand, within BT2663 at 3319.678 kb on + strand, within BT2663 at 3319.679 kb on - strand, within BT2663 at 3319.837 kb on + strand, within BT2663 at 3319.896 kb on - strand, within BT2663 at 3319.899 kb on + strand, within BT2663 at 3319.900 kb on - strand, within BT2663 at 3319.976 kb on - strand, within BT2663 at 3319.987 kb on + strand, within BT2663 at 3319.997 kb on - strand, within BT2663 at 3319.997 kb on - strand, within BT2663 at 3320.027 kb on + strand, within BT2663 at 3320.143 kb on + strand, within BT2663 at 3320.143 kb on + strand, within BT2663 at 3320.144 kb on - strand, within BT2663 at 3320.181 kb on + strand, within BT2663 at 3320.232 kb on + strand at 3320.336 kb on - strand at 3320.393 kb on + strand at 3320.413 kb on + strand at 3320.435 kb on + strand at 3320.475 kb on + strand at 3320.505 kb on - strand at 3320.557 kb on + strand, within BT2664 at 3320.579 kb on - strand, within BT2664 at 3320.591 kb on - strand, within BT2664 at 3320.597 kb on - strand, within BT2664 at 3320.612 kb on + strand, within BT2664 at 3320.612 kb on + strand, within BT2664 at 3320.615 kb on - strand, within BT2664 at 3320.634 kb on + strand, within BT2664 at 3320.635 kb on - strand, within BT2664 at 3320.659 kb on - strand, within BT2664 at 3320.759 kb on - strand, within BT2664 at 3320.847 kb on + strand, within BT2664 at 3320.864 kb on + strand, within BT2664 at 3320.885 kb on + strand, within BT2664 at 3320.885 kb on + strand, within BT2664 at 3320.911 kb on - strand, within BT2664 at 3321.000 kb on + strand, within BT2664 at 3321.017 kb on + strand, within BT2664 at 3321.127 kb on - strand, within BT2664 at 3321.131 kb on + strand, within BT2664 at 3321.218 kb on + strand, within BT2664 at 3321.219 kb on - strand, within BT2664 at 3321.265 kb on + strand at 3321.266 kb on - strand at 3321.330 kb on + strand at 3321.359 kb on + strand at 3321.359 kb on + strand at 3321.360 kb on - strand at 3321.369 kb on + strand at 3321.442 kb on - strand, within BT2665 at 3321.442 kb on - strand, within BT2665 at 3321.442 kb on - strand, within BT2665 at 3321.468 kb on + strand, within BT2665 at 3321.472 kb on + strand, within BT2665 at 3321.473 kb on - strand, within BT2665 at 3321.497 kb on - strand, within BT2665 at 3321.497 kb on - strand, within BT2665 at 3321.513 kb on + strand, within BT2665 at 3321.513 kb on + strand, within BT2665 at 3321.516 kb on + strand, within BT2665 at 3321.529 kb on + strand, within BT2665 at 3321.587 kb on + strand, within BT2665 at 3321.608 kb on + strand, within BT2665 at 3321.660 kb on + strand, within BT2665 at 3321.661 kb on - strand, within BT2665 at 3321.742 kb on - strand, within BT2665 at 3321.777 kb on + strand, within BT2665 at 3321.858 kb on + strand, within BT2665 at 3321.971 kb on - strand, within BT2665 at 3322.009 kb on - strand, within BT2665 at 3322.059 kb on + strand, within BT2665 at 3322.062 kb on + strand, within BT2665 at 3322.062 kb on + strand, within BT2665 at 3322.062 kb on + strand, within BT2665 at 3322.063 kb on - strand, within BT2665 at 3322.092 kb on + strand at 3322.193 kb on + strand at 3322.193 kb on + strand at 3322.194 kb on - strand at 3322.288 kb on + strand, within BT2666 at 3322.292 kb on + strand, within BT2666 at 3322.293 kb on - strand, within BT2666 at 3322.296 kb on + strand, within BT2666 at 3322.297 kb on - strand, within BT2666 at 3322.298 kb on + strand, within BT2666 at 3322.298 kb on + strand, within BT2666 at 3322.301 kb on + strand, within BT2666 at 3322.301 kb on + strand, within BT2666 at 3322.301 kb on + strand, within BT2666 at 3322.301 kb on + strand, within BT2666 at 3322.302 kb on - strand, within BT2666 at 3322.305 kb on + strand, within BT2666
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Vancomycin 0.016 mg/ml remove 3,319,442 - BT2663 0.36 +3.0 3,319,482 + BT2663 0.38 +1.2 3,319,504 - BT2663 0.40 +3.2 3,319,509 + BT2663 0.40 -1.0 3,319,523 - BT2663 0.41 +3.7 3,319,576 + BT2663 0.45 -2.1 3,319,577 - BT2663 0.45 +3.6 3,319,581 - BT2663 0.45 +2.9 3,319,581 - BT2663 0.45 +3.9 3,319,582 + BT2663 0.45 -0.4 3,319,582 + BT2663 0.45 -1.1 3,319,582 + BT2663 0.45 +0.0 3,319,591 + BT2663 0.46 +1.0 3,319,642 - BT2663 0.49 +2.9 3,319,672 + BT2663 0.52 +0.3 3,319,673 - BT2663 0.52 +3.3 3,319,673 - BT2663 0.52 +3.9 3,319,676 + BT2663 0.52 -0.5 3,319,676 + BT2663 0.52 +1.8 3,319,677 - BT2663 0.52 +2.7 3,319,678 + BT2663 0.52 +0.3 3,319,678 + BT2663 0.52 -0.0 3,319,679 - BT2663 0.52 +2.2 3,319,837 + BT2663 0.63 +1.4 3,319,896 - BT2663 0.67 +2.4 3,319,899 + BT2663 0.67 -1.3 3,319,900 - BT2663 0.67 +1.2 3,319,976 - BT2663 0.72 +2.3 3,319,987 + BT2663 0.73 -0.3 3,319,997 - BT2663 0.74 +3.3 3,319,997 - BT2663 0.74 +2.0 3,320,027 + BT2663 0.76 +1.2 3,320,143 + BT2663 0.84 +0.2 3,320,143 + BT2663 0.84 -1.2 3,320,144 - BT2663 0.84 +3.5 3,320,181 + BT2663 0.87 -2.6 3,320,232 + -0.6 3,320,336 - +2.0 3,320,393 + +2.0 3,320,413 + +0.2 3,320,435 + +0.2 3,320,475 + +1.2 3,320,505 - +3.4 3,320,557 + BT2664 0.15 -0.3 3,320,579 - BT2664 0.18 +3.4 3,320,591 - BT2664 0.19 +2.4 3,320,597 - BT2664 0.20 +2.8 3,320,612 + BT2664 0.21 +0.3 3,320,612 + BT2664 0.21 -1.3 3,320,615 - BT2664 0.21 +2.7 3,320,634 + BT2664 0.23 -2.1 3,320,635 - BT2664 0.24 +2.6 3,320,659 - BT2664 0.26 +2.0 3,320,759 - BT2664 0.37 +0.4 3,320,847 + BT2664 0.46 -0.2 3,320,864 + BT2664 0.48 +0.1 3,320,885 + BT2664 0.50 -0.8 3,320,885 + BT2664 0.50 +0.0 3,320,911 - BT2664 0.53 +1.4 3,321,000 + BT2664 0.62 -1.1 3,321,017 + BT2664 0.64 +0.6 3,321,127 - BT2664 0.76 +0.8 3,321,131 + BT2664 0.76 +0.7 3,321,218 + BT2664 0.85 -0.9 3,321,219 - BT2664 0.85 -0.8 3,321,265 + -0.8 3,321,266 - +0.9 3,321,330 + +2.2 3,321,359 + +0.3 3,321,359 + +0.0 3,321,360 - +0.8 3,321,369 + -2.3 3,321,442 - BT2665 0.10 +2.5 3,321,442 - BT2665 0.10 +4.3 3,321,442 - BT2665 0.10 +2.3 3,321,468 + BT2665 0.14 +3.2 3,321,472 + BT2665 0.14 -0.4 3,321,473 - BT2665 0.14 +2.1 3,321,497 - BT2665 0.17 +0.5 3,321,497 - BT2665 0.17 +0.7 3,321,513 + BT2665 0.19 +0.6 3,321,513 + BT2665 0.19 -0.6 3,321,516 + BT2665 0.19 +0.2 3,321,529 + BT2665 0.21 -1.3 3,321,587 + BT2665 0.28 -0.8 3,321,608 + BT2665 0.31 +0.6 3,321,660 + BT2665 0.37 +0.5 3,321,661 - BT2665 0.37 +1.5 3,321,742 - BT2665 0.47 +2.8 3,321,777 + BT2665 0.52 -2.2 3,321,858 + BT2665 0.62 -1.1 3,321,971 - BT2665 0.75 +3.6 3,322,009 - BT2665 0.80 +1.3 3,322,059 + BT2665 0.86 +0.2 3,322,062 + BT2665 0.87 +1.2 3,322,062 + BT2665 0.87 -0.7 3,322,062 + BT2665 0.87 -1.8 3,322,063 - BT2665 0.87 +1.7 3,322,092 + -1.2 3,322,193 + -0.1 3,322,193 + -0.6 3,322,194 - +2.9 3,322,288 + BT2666 0.14 +1.7 3,322,292 + BT2666 0.15 +0.9 3,322,293 - BT2666 0.15 +2.5 3,322,296 + BT2666 0.15 +0.1 3,322,297 - BT2666 0.15 +0.2 3,322,298 + BT2666 0.15 +0.6 3,322,298 + BT2666 0.15 +0.7 3,322,301 + BT2666 0.16 +0.2 3,322,301 + BT2666 0.16 -0.8 3,322,301 + BT2666 0.16 +0.0 3,322,301 + BT2666 0.16 +0.0 3,322,302 - BT2666 0.16 +4.0 3,322,305 + BT2666 0.17 -1.0
Or see this region's nucleotide sequence