Experiment: BHIS with Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0238 and BT0239 are separated by 157 nucleotides BT0239 and BT0240 are separated by 43 nucleotides BT0240 and BT0241 are separated by 165 nucleotides BT0241 and BT0242 are separated by 29 nucleotides
BT0238: BT0238 - transcriptional regulator (NCBI ptt file), at 262,126 to 263,370
BT0238
BT0239: BT0239 - hypothetical protein (NCBI ptt file), at 263,528 to 264,262
BT0239
BT0240: BT0240 - conserved hypothetical protein (NCBI ptt file), at 264,306 to 266,060
BT0240
BT0241: BT0241 - hypothetical protein (NCBI ptt file), at 266,226 to 266,609
BT0241
BT0242: BT0242 - putative polysaccharide deacetylase (NCBI ptt file), at 266,639 to 267,115
BT0242
Position (kb) 264
265
266
267 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 263.354 kb on + strand at 263.354 kb on + strand at 263.391 kb on + strand at 263.435 kb on - strand at 263.539 kb on + strand at 263.590 kb on + strand at 263.590 kb on + strand at 263.590 kb on + strand at 263.590 kb on + strand at 263.590 kb on + strand at 263.590 kb on + strand at 263.591 kb on - strand at 263.591 kb on - strand at 263.591 kb on - strand at 263.594 kb on + strand at 263.596 kb on + strand at 263.601 kb on + strand at 263.601 kb on + strand at 263.602 kb on - strand, within BT0239 at 263.602 kb on - strand, within BT0239 at 263.620 kb on - strand, within BT0239 at 263.645 kb on + strand, within BT0239 at 263.812 kb on + strand, within BT0239 at 263.818 kb on + strand, within BT0239 at 263.875 kb on + strand, within BT0239 at 264.021 kb on - strand, within BT0239 at 264.067 kb on + strand, within BT0239 at 264.072 kb on + strand, within BT0239 at 264.073 kb on - strand, within BT0239 at 264.076 kb on + strand, within BT0239 at 264.076 kb on + strand, within BT0239 at 264.078 kb on + strand, within BT0239 at 264.102 kb on - strand, within BT0239 at 264.106 kb on + strand, within BT0239 at 264.109 kb on + strand, within BT0239 at 264.123 kb on + strand, within BT0239 at 264.124 kb on - strand, within BT0239 at 264.163 kb on + strand, within BT0239 at 264.167 kb on - strand, within BT0239 at 264.374 kb on + strand at 264.380 kb on + strand at 264.545 kb on + strand, within BT0240 at 264.577 kb on + strand, within BT0240 at 264.594 kb on - strand, within BT0240 at 264.594 kb on - strand, within BT0240 at 264.622 kb on - strand, within BT0240 at 264.726 kb on - strand, within BT0240 at 264.835 kb on - strand, within BT0240 at 264.903 kb on - strand, within BT0240 at 264.931 kb on - strand, within BT0240 at 265.007 kb on - strand, within BT0240 at 265.007 kb on - strand, within BT0240 at 265.012 kb on - strand, within BT0240 at 265.121 kb on - strand, within BT0240 at 265.122 kb on + strand, within BT0240 at 265.397 kb on + strand, within BT0240 at 265.449 kb on - strand, within BT0240 at 265.465 kb on - strand, within BT0240 at 265.534 kb on + strand, within BT0240 at 265.555 kb on + strand, within BT0240 at 265.556 kb on - strand, within BT0240 at 265.562 kb on + strand, within BT0240 at 265.622 kb on + strand, within BT0240 at 265.657 kb on + strand, within BT0240 at 265.742 kb on - strand, within BT0240 at 265.819 kb on + strand, within BT0240 at 265.946 kb on - strand at 266.382 kb on - strand, within BT0241 at 266.459 kb on - strand, within BT0241 at 266.524 kb on + strand, within BT0241 at 266.634 kb on - strand at 266.638 kb on - strand at 266.662 kb on + strand at 266.663 kb on - strand at 266.695 kb on + strand, within BT0242 at 266.696 kb on - strand, within BT0242 at 266.704 kb on + strand, within BT0242 at 266.763 kb on + strand, within BT0242 at 266.818 kb on - strand, within BT0242 at 266.935 kb on + strand, within BT0242 at 267.036 kb on + strand, within BT0242
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Vancomycin 0.016 mg/ml remove 263,354 + +0.3 263,354 + -0.2 263,391 + +0.2 263,435 - -4.8 263,539 + +0.7 263,590 + +0.1 263,590 + -2.6 263,590 + +2.1 263,590 + +1.3 263,590 + +1.1 263,590 + +0.4 263,591 - +0.8 263,591 - +0.8 263,591 - +0.1 263,594 + +1.4 263,596 + +1.5 263,601 + -0.6 263,601 + +0.1 263,602 - BT0239 0.10 +1.1 263,602 - BT0239 0.10 +1.4 263,620 - BT0239 0.13 -1.2 263,645 + BT0239 0.16 +2.0 263,812 + BT0239 0.39 -0.2 263,818 + BT0239 0.39 +0.4 263,875 + BT0239 0.47 +1.9 264,021 - BT0239 0.67 +1.0 264,067 + BT0239 0.73 +0.1 264,072 + BT0239 0.74 -0.2 264,073 - BT0239 0.74 +2.9 264,076 + BT0239 0.75 -0.5 264,076 + BT0239 0.75 +1.8 264,078 + BT0239 0.75 +0.3 264,102 - BT0239 0.78 +0.7 264,106 + BT0239 0.79 +2.3 264,109 + BT0239 0.79 +1.3 264,123 + BT0239 0.81 +1.6 264,124 - BT0239 0.81 +1.5 264,163 + BT0239 0.86 +1.1 264,167 - BT0239 0.87 +1.3 264,374 + +3.7 264,380 + +2.3 264,545 + BT0240 0.14 +2.8 264,577 + BT0240 0.15 +1.8 264,594 - BT0240 0.16 +2.5 264,594 - BT0240 0.16 +1.7 264,622 - BT0240 0.18 +2.6 264,726 - BT0240 0.24 +2.0 264,835 - BT0240 0.30 +0.6 264,903 - BT0240 0.34 +0.8 264,931 - BT0240 0.36 -1.2 265,007 - BT0240 0.40 +2.7 265,007 - BT0240 0.40 -1.0 265,012 - BT0240 0.40 +0.1 265,121 - BT0240 0.46 +1.7 265,122 + BT0240 0.46 +0.8 265,397 + BT0240 0.62 +2.9 265,449 - BT0240 0.65 +1.3 265,465 - BT0240 0.66 +2.0 265,534 + BT0240 0.70 +0.4 265,555 + BT0240 0.71 +1.6 265,556 - BT0240 0.71 +1.7 265,562 + BT0240 0.72 +1.3 265,622 + BT0240 0.75 -0.6 265,657 + BT0240 0.77 +1.6 265,742 - BT0240 0.82 +1.2 265,819 + BT0240 0.86 +0.4 265,946 - -0.8 266,382 - BT0241 0.41 -1.2 266,459 - BT0241 0.61 -1.6 266,524 + BT0241 0.78 +1.1 266,634 - -1.5 266,638 - +3.9 266,662 + +2.0 266,663 - +1.5 266,695 + BT0242 0.12 -2.0 266,696 - BT0242 0.12 +2.3 266,704 + BT0242 0.14 -0.8 266,763 + BT0242 0.26 +4.1 266,818 - BT0242 0.38 -1.3 266,935 + BT0242 0.62 +1.1 267,036 + BT0242 0.83 -0.3
Or see this region's nucleotide sequence