Experiment: BHIS with Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0066 and BT0067 are separated by 337 nucleotides BT0067 and BT0068 are separated by 27 nucleotides BT0068 and BT0069 are separated by 284 nucleotides
BT0066: BT0066 - major outer membrane protein OmpA (NCBI ptt file), at 64,273 to 65,475
BT0066
BT0067: BT0067 - hypothetical protein (NCBI ptt file), at 65,813 to 66,064
BT0067
BT0068: BT0068 - hypothetical protein (NCBI ptt file), at 66,092 to 66,571
BT0068
BT0069: BT0069 - conserved hypothetical protein (NCBI ptt file), at 66,856 to 67,662
BT0069
Position (kb) 66
67 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 65.261 kb on + strand, within BT0066 at 65.266 kb on - strand, within BT0066 at 65.266 kb on - strand, within BT0066 at 65.266 kb on - strand, within BT0066 at 65.474 kb on - strand at 65.474 kb on - strand at 65.491 kb on - strand at 65.491 kb on - strand at 65.583 kb on - strand at 65.672 kb on - strand at 65.672 kb on - strand at 65.700 kb on - strand at 65.791 kb on - strand at 65.805 kb on - strand at 65.812 kb on + strand at 65.823 kb on + strand at 65.824 kb on - strand at 65.824 kb on - strand at 65.994 kb on - strand, within BT0067 at 65.999 kb on + strand, within BT0067 at 66.066 kb on - strand at 66.136 kb on + strand at 66.255 kb on + strand, within BT0068 at 66.438 kb on - strand, within BT0068 at 66.474 kb on - strand, within BT0068 at 66.532 kb on + strand at 66.533 kb on - strand at 66.576 kb on + strand at 66.625 kb on - strand at 66.784 kb on + strand at 66.785 kb on - strand at 66.805 kb on + strand at 66.822 kb on - strand at 66.857 kb on + strand at 66.857 kb on + strand at 66.863 kb on + strand at 66.864 kb on - strand at 66.895 kb on + strand at 66.895 kb on + strand at 66.895 kb on + strand at 66.895 kb on + strand at 66.898 kb on + strand at 66.904 kb on + strand at 66.936 kb on + strand at 66.936 kb on + strand at 66.951 kb on - strand, within BT0069 at 66.959 kb on - strand, within BT0069 at 66.991 kb on + strand, within BT0069 at 67.000 kb on + strand, within BT0069 at 67.001 kb on - strand, within BT0069 at 67.020 kb on + strand, within BT0069 at 67.043 kb on - strand, within BT0069 at 67.077 kb on - strand, within BT0069 at 67.102 kb on - strand, within BT0069 at 67.102 kb on - strand, within BT0069 at 67.103 kb on + strand, within BT0069 at 67.104 kb on - strand, within BT0069 at 67.112 kb on - strand, within BT0069 at 67.112 kb on - strand, within BT0069 at 67.209 kb on - strand, within BT0069 at 67.212 kb on + strand, within BT0069 at 67.258 kb on + strand, within BT0069 at 67.262 kb on - strand, within BT0069 at 67.280 kb on - strand, within BT0069 at 67.280 kb on - strand, within BT0069 at 67.280 kb on - strand, within BT0069 at 67.281 kb on + strand, within BT0069 at 67.281 kb on + strand, within BT0069 at 67.284 kb on - strand, within BT0069 at 67.286 kb on + strand, within BT0069 at 67.417 kb on - strand, within BT0069
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Vancomycin 0.016 mg/ml remove 65,261 + BT0066 0.82 +0.8 65,266 - BT0066 0.83 -0.5 65,266 - BT0066 0.83 +1.2 65,266 - BT0066 0.83 -1.8 65,474 - -0.5 65,474 - -0.5 65,491 - -0.4 65,491 - -0.7 65,583 - -0.7 65,672 - -0.2 65,672 - -1.2 65,700 - -1.6 65,791 - +0.1 65,805 - +0.8 65,812 + +0.5 65,823 + -2.6 65,824 - +0.2 65,824 - -0.6 65,994 - BT0067 0.72 -0.4 65,999 + BT0067 0.74 -0.7 66,066 - -1.3 66,136 + +1.3 66,255 + BT0068 0.34 +0.4 66,438 - BT0068 0.72 -2.9 66,474 - BT0068 0.80 -2.4 66,532 + -0.7 66,533 - +0.4 66,576 + -0.6 66,625 - +1.9 66,784 + -0.4 66,785 - +0.3 66,805 + +2.2 66,822 - -0.8 66,857 + -0.2 66,857 + +0.8 66,863 + -1.2 66,864 - -0.6 66,895 + +1.8 66,895 + -2.0 66,895 + +0.5 66,895 + +0.8 66,898 + +0.4 66,904 + +0.7 66,936 + -1.0 66,936 + -2.1 66,951 - BT0069 0.12 +0.2 66,959 - BT0069 0.13 -0.9 66,991 + BT0069 0.17 -0.2 67,000 + BT0069 0.18 +1.4 67,001 - BT0069 0.18 -1.5 67,020 + BT0069 0.20 +0.3 67,043 - BT0069 0.23 -0.2 67,077 - BT0069 0.27 +0.9 67,102 - BT0069 0.30 +1.4 67,102 - BT0069 0.30 +1.2 67,103 + BT0069 0.31 +0.2 67,104 - BT0069 0.31 -1.2 67,112 - BT0069 0.32 +0.7 67,112 - BT0069 0.32 +1.0 67,209 - BT0069 0.44 +0.8 67,212 + BT0069 0.44 +0.0 67,258 + BT0069 0.50 +0.5 67,262 - BT0069 0.50 -2.0 67,280 - BT0069 0.53 +0.8 67,280 - BT0069 0.53 -0.6 67,280 - BT0069 0.53 +1.8 67,281 + BT0069 0.53 -0.4 67,281 + BT0069 0.53 -0.8 67,284 - BT0069 0.53 -1.6 67,286 + BT0069 0.53 -0.2 67,417 - BT0069 0.70 +0.6
Or see this region's nucleotide sequence