Experiment: MoLS4 with L-Serine (N), without W
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbA and rfbC overlap by 4 nucleotides rfbC and DVU0699 are separated by 176 nucleotides DVU0699 and tRNA-Ser are separated by 56 nucleotides tRNA-Ser and DVU0700 are separated by 264 nucleotides
DVU0697: rfbA - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (TIGR), at 771,808 to 773,226
rfbA
DVU0698: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase (TIGR), at 773,223 to 773,795
rfbC
DVU0699: DVU0699 - sensory box sensor histidine kinase/response regulator, authentic frameshift (TIGR), at 773,972 to 775,650
DVU0699
DVU_tRNA-Ser-1: tRNA-Ser - tRNA with anticodon TGA for Ser (VIMSS-AUTO), at 775,707 to 775,798
tRNA-Ser
DVU0700: DVU0700 - methyl-accepting chemotaxis protein (TIGR), at 776,063 to 778,519
DVU0700
Position (kb) 773
774
775
776 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 773.386 kb on - strand, within rfbC at 773.395 kb on + strand, within rfbC at 773.482 kb on - strand, within rfbC at 773.497 kb on - strand, within rfbC at 773.542 kb on - strand, within rfbC at 773.572 kb on - strand, within rfbC at 773.577 kb on - strand, within rfbC at 773.878 kb on - strand at 773.882 kb on + strand at 773.981 kb on + strand at 773.983 kb on + strand at 774.054 kb on - strand at 774.054 kb on - strand at 774.060 kb on - strand at 774.068 kb on + strand at 774.123 kb on + strand at 774.123 kb on + strand at 774.131 kb on - strand at 774.131 kb on - strand at 774.135 kb on + strand at 774.151 kb on + strand, within DVU0699 at 774.158 kb on + strand, within DVU0699 at 774.208 kb on + strand, within DVU0699 at 774.286 kb on + strand, within DVU0699 at 774.323 kb on + strand, within DVU0699 at 774.375 kb on - strand, within DVU0699 at 774.460 kb on + strand, within DVU0699 at 774.540 kb on + strand, within DVU0699 at 774.541 kb on - strand, within DVU0699 at 774.564 kb on + strand, within DVU0699 at 774.567 kb on - strand, within DVU0699 at 774.567 kb on - strand, within DVU0699 at 774.593 kb on + strand, within DVU0699 at 774.685 kb on + strand, within DVU0699 at 774.706 kb on + strand, within DVU0699 at 774.706 kb on + strand, within DVU0699 at 774.716 kb on - strand, within DVU0699 at 774.844 kb on + strand, within DVU0699 at 774.844 kb on + strand, within DVU0699 at 774.877 kb on + strand, within DVU0699 at 774.877 kb on + strand, within DVU0699 at 774.905 kb on + strand, within DVU0699 at 775.016 kb on + strand, within DVU0699 at 775.021 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.059 kb on + strand, within DVU0699 at 775.066 kb on - strand, within DVU0699 at 775.066 kb on - strand, within DVU0699 at 775.078 kb on - strand, within DVU0699 at 775.182 kb on - strand, within DVU0699 at 775.257 kb on + strand, within DVU0699 at 775.312 kb on + strand, within DVU0699 at 775.313 kb on - strand, within DVU0699 at 775.313 kb on - strand, within DVU0699 at 775.373 kb on + strand, within DVU0699 at 775.381 kb on - strand, within DVU0699 at 775.402 kb on + strand, within DVU0699 at 775.516 kb on - strand at 775.705 kb on + strand at 776.069 kb on - strand at 776.113 kb on + strand at 776.115 kb on - strand at 776.123 kb on + strand at 776.123 kb on + strand at 776.131 kb on - strand at 776.167 kb on - strand at 776.170 kb on + strand at 776.384 kb on - strand, within DVU0700 at 776.402 kb on + strand, within DVU0700 at 776.412 kb on + strand, within DVU0700 at 776.475 kb on - strand, within DVU0700 at 776.574 kb on + strand, within DVU0700 at 776.587 kb on - strand, within DVU0700 at 776.632 kb on - strand, within DVU0700 at 776.632 kb on - strand, within DVU0700 at 776.634 kb on + strand, within DVU0700 at 776.635 kb on + strand, within DVU0700 at 776.645 kb on + strand, within DVU0700
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Serine (N), without W remove 773,386 - rfbC DVU0698 0.28 -1.0 773,395 + rfbC DVU0698 0.30 -2.3 773,482 - rfbC DVU0698 0.45 -0.8 773,497 - rfbC DVU0698 0.48 -1.2 773,542 - rfbC DVU0698 0.56 -0.3 773,572 - rfbC DVU0698 0.61 -2.2 773,577 - rfbC DVU0698 0.62 -1.6 773,878 - -0.6 773,882 + -0.9 773,981 + -0.2 773,983 + -0.4 774,054 - +0.2 774,054 - -2.0 774,060 - +0.5 774,068 + -0.4 774,123 + -0.0 774,123 + +0.1 774,131 - -0.5 774,131 - +0.5 774,135 + -0.1 774,151 + DVU0699 0.11 +0.1 774,158 + DVU0699 0.11 +0.1 774,208 + DVU0699 0.14 -0.4 774,286 + DVU0699 0.19 -0.7 774,323 + DVU0699 0.21 -0.2 774,375 - DVU0699 0.24 +0.0 774,460 + DVU0699 0.29 -1.5 774,540 + DVU0699 0.34 -0.2 774,541 - DVU0699 0.34 +0.2 774,564 + DVU0699 0.35 +0.3 774,567 - DVU0699 0.35 -0.0 774,567 - DVU0699 0.35 -1.1 774,593 + DVU0699 0.37 +0.3 774,685 + DVU0699 0.42 +0.9 774,706 + DVU0699 0.44 +0.2 774,706 + DVU0699 0.44 -0.1 774,716 - DVU0699 0.44 +0.3 774,844 + DVU0699 0.52 -0.9 774,844 + DVU0699 0.52 +0.1 774,877 + DVU0699 0.54 -0.3 774,877 + DVU0699 0.54 -0.3 774,905 + DVU0699 0.56 +1.4 775,016 + DVU0699 0.62 +0.6 775,021 + DVU0699 0.62 -0.0 775,058 + DVU0699 0.65 -0.7 775,058 + DVU0699 0.65 +0.1 775,058 + DVU0699 0.65 -0.0 775,058 + DVU0699 0.65 -1.8 775,058 + DVU0699 0.65 -0.1 775,058 + DVU0699 0.65 -0.5 775,059 + DVU0699 0.65 -0.9 775,066 - DVU0699 0.65 +1.4 775,066 - DVU0699 0.65 +0.1 775,078 - DVU0699 0.66 +0.5 775,182 - DVU0699 0.72 +0.0 775,257 + DVU0699 0.77 -0.0 775,312 + DVU0699 0.80 +2.1 775,313 - DVU0699 0.80 -0.5 775,313 - DVU0699 0.80 +0.4 775,373 + DVU0699 0.83 +3.2 775,381 - DVU0699 0.84 -0.1 775,402 + DVU0699 0.85 -0.7 775,516 - -0.4 775,705 + -0.5 776,069 - -0.1 776,113 + -0.0 776,115 - -0.5 776,123 + +0.0 776,123 + -0.2 776,131 - +0.0 776,167 - -0.2 776,170 + +0.1 776,384 - DVU0700 0.13 -0.3 776,402 + DVU0700 0.14 -0.1 776,412 + DVU0700 0.14 +0.1 776,475 - DVU0700 0.17 -1.2 776,574 + DVU0700 0.21 +0.0 776,587 - DVU0700 0.21 -0.2 776,632 - DVU0700 0.23 -0.3 776,632 - DVU0700 0.23 -1.0 776,634 + DVU0700 0.23 -0.2 776,635 + DVU0700 0.23 -0.4 776,645 + DVU0700 0.24 +0.4
Or see this region's nucleotide sequence