Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08615 and MMJJ_RS08620 are separated by 28 nucleotides MMJJ_RS08620 and MMJJ_RS08625 are separated by 10 nucleotides MMJJ_RS08625 and MMJJ_RS08630 are separated by 13 nucleotides
MMJJ_RS08615: MMJJ_RS08615 - oligosaccharide repeat unit polymerase family protein, at 1,586,456 to 1,587,631
_RS08615
MMJJ_RS08620: MMJJ_RS08620 - DUF373 family protein, at 1,587,660 to 1,588,769
_RS08620
MMJJ_RS08625: MMJJ_RS08625 - magnesium/cobalt transporter CorA, at 1,588,780 to 1,589,730
_RS08625
MMJJ_RS08630: MMJJ_RS08630 - signal recognition particle subunit SRP19/SEC65 family protein, at 1,589,744 to 1,590,013
_RS08630
Position (kb)
1587
1588
1589 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1586.691 kb on - strand, within MMJJ_RS08615 at 1586.775 kb on - strand, within MMJJ_RS08615 at 1586.805 kb on - strand, within MMJJ_RS08615 at 1586.806 kb on + strand, within MMJJ_RS08615 at 1586.838 kb on + strand, within MMJJ_RS08615 at 1586.871 kb on - strand, within MMJJ_RS08615 at 1586.881 kb on + strand, within MMJJ_RS08615 at 1586.881 kb on + strand, within MMJJ_RS08615 at 1586.905 kb on + strand, within MMJJ_RS08615 at 1586.905 kb on + strand, within MMJJ_RS08615 at 1586.967 kb on - strand, within MMJJ_RS08615 at 1587.020 kb on - strand, within MMJJ_RS08615 at 1587.031 kb on + strand, within MMJJ_RS08615 at 1587.081 kb on - strand, within MMJJ_RS08615 at 1587.082 kb on + strand, within MMJJ_RS08615 at 1587.103 kb on - strand, within MMJJ_RS08615 at 1587.153 kb on - strand, within MMJJ_RS08615 at 1587.154 kb on + strand, within MMJJ_RS08615 at 1587.157 kb on - strand at 1587.273 kb on - strand, within MMJJ_RS08615 at 1587.274 kb on + strand, within MMJJ_RS08615 at 1587.283 kb on - strand, within MMJJ_RS08615 at 1587.286 kb on + strand, within MMJJ_RS08615 at 1587.323 kb on + strand, within MMJJ_RS08615 at 1587.354 kb on - strand, within MMJJ_RS08615 at 1587.354 kb on - strand, within MMJJ_RS08615 at 1587.373 kb on - strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.376 kb on - strand, within MMJJ_RS08615 at 1587.377 kb on + strand, within MMJJ_RS08615 at 1587.380 kb on + strand, within MMJJ_RS08615 at 1587.405 kb on + strand, within MMJJ_RS08615 at 1587.429 kb on - strand, within MMJJ_RS08615 at 1587.445 kb on + strand, within MMJJ_RS08615 at 1587.445 kb on + strand, within MMJJ_RS08615 at 1587.460 kb on - strand, within MMJJ_RS08615 at 1587.465 kb on - strand, within MMJJ_RS08615 at 1587.466 kb on + strand, within MMJJ_RS08615 at 1587.471 kb on + strand, within MMJJ_RS08615 at 1587.514 kb on + strand at 1587.659 kb on + strand at 1587.671 kb on + strand at 1587.886 kb on - strand, within MMJJ_RS08620 at 1587.952 kb on - strand, within MMJJ_RS08620 at 1588.008 kb on + strand, within MMJJ_RS08620 at 1588.326 kb on + strand, within MMJJ_RS08620 at 1588.402 kb on - strand at 1588.408 kb on - strand, within MMJJ_RS08620 at 1588.445 kb on + strand, within MMJJ_RS08620 at 1588.475 kb on - strand, within MMJJ_RS08620 at 1588.476 kb on + strand, within MMJJ_RS08620 at 1588.528 kb on - strand, within MMJJ_RS08620 at 1588.540 kb on - strand, within MMJJ_RS08620 at 1588.653 kb on + strand at 1588.681 kb on + strand at 1588.681 kb on + strand at 1588.688 kb on + strand at 1588.688 kb on + strand at 1588.696 kb on - strand at 1588.697 kb on + strand at 1588.697 kb on + strand at 1588.697 kb on + strand at 1588.697 kb on + strand at 1588.697 kb on + strand at 1588.705 kb on - strand at 1588.705 kb on - strand at 1588.706 kb on + strand at 1588.706 kb on + strand at 1588.767 kb on - strand at 1588.838 kb on - strand at 1588.839 kb on + strand at 1588.839 kb on + strand at 1588.843 kb on + strand at 1588.857 kb on - strand at 1588.874 kb on + strand at 1588.916 kb on - strand, within MMJJ_RS08625 at 1588.986 kb on - strand, within MMJJ_RS08625 at 1588.986 kb on - strand, within MMJJ_RS08625 at 1588.987 kb on + strand, within MMJJ_RS08625 at 1588.990 kb on - strand, within MMJJ_RS08625 at 1588.990 kb on - strand, within MMJJ_RS08625 at 1588.994 kb on + strand, within MMJJ_RS08625 at 1588.995 kb on - strand, within MMJJ_RS08625 at 1588.996 kb on + strand, within MMJJ_RS08625 at 1589.001 kb on - strand, within MMJJ_RS08625 at 1589.040 kb on - strand, within MMJJ_RS08625 at 1589.060 kb on - strand, within MMJJ_RS08625 at 1589.229 kb on + strand, within MMJJ_RS08625 at 1589.255 kb on - strand, within MMJJ_RS08625 at 1589.306 kb on - strand, within MMJJ_RS08625 at 1589.346 kb on - strand, within MMJJ_RS08625 at 1589.346 kb on - strand, within MMJJ_RS08625 at 1589.346 kb on - strand, within MMJJ_RS08625 at 1589.346 kb on - strand, within MMJJ_RS08625 at 1589.348 kb on - strand, within MMJJ_RS08625 at 1589.364 kb on - strand, within MMJJ_RS08625 at 1589.365 kb on + strand, within MMJJ_RS08625 at 1589.412 kb on - strand, within MMJJ_RS08625 at 1589.412 kb on - strand, within MMJJ_RS08625 at 1589.413 kb on + strand, within MMJJ_RS08625 at 1589.413 kb on + strand at 1589.432 kb on - strand, within MMJJ_RS08625 at 1589.437 kb on - strand, within MMJJ_RS08625 at 1589.474 kb on + strand, within MMJJ_RS08625 at 1589.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,586,691 - MMJJ_RS08615 0.20 +0.3 1,586,775 - MMJJ_RS08615 0.27 -1.9 1,586,805 - MMJJ_RS08615 0.30 -1.0 1,586,806 + MMJJ_RS08615 0.30 -1.6 1,586,838 + MMJJ_RS08615 0.32 +0.8 1,586,871 - MMJJ_RS08615 0.35 -1.4 1,586,881 + MMJJ_RS08615 0.36 -0.9 1,586,881 + MMJJ_RS08615 0.36 +0.9 1,586,905 + MMJJ_RS08615 0.38 -0.0 1,586,905 + MMJJ_RS08615 0.38 +0.3 1,586,967 - MMJJ_RS08615 0.43 -0.1 1,587,020 - MMJJ_RS08615 0.48 -0.2 1,587,031 + MMJJ_RS08615 0.49 -1.7 1,587,081 - MMJJ_RS08615 0.53 -0.9 1,587,082 + MMJJ_RS08615 0.53 -0.1 1,587,103 - MMJJ_RS08615 0.55 +0.4 1,587,153 - MMJJ_RS08615 0.59 +0.5 1,587,154 + MMJJ_RS08615 0.59 -0.4 1,587,157 - +0.1 1,587,273 - MMJJ_RS08615 0.69 -1.8 1,587,274 + MMJJ_RS08615 0.70 +0.5 1,587,283 - MMJJ_RS08615 0.70 -1.0 1,587,286 + MMJJ_RS08615 0.71 -0.1 1,587,323 + MMJJ_RS08615 0.74 -1.7 1,587,354 - MMJJ_RS08615 0.76 -1.1 1,587,354 - MMJJ_RS08615 0.76 -2.5 1,587,373 - MMJJ_RS08615 0.78 -0.1 1,587,374 + MMJJ_RS08615 0.78 -0.8 1,587,374 + MMJJ_RS08615 0.78 -1.7 1,587,374 + MMJJ_RS08615 0.78 +0.8 1,587,376 - MMJJ_RS08615 0.78 -0.2 1,587,377 + MMJJ_RS08615 0.78 -0.4 1,587,380 + MMJJ_RS08615 0.79 -1.7 1,587,405 + MMJJ_RS08615 0.81 -2.4 1,587,429 - MMJJ_RS08615 0.83 -0.4 1,587,445 + MMJJ_RS08615 0.84 -2.7 1,587,445 + MMJJ_RS08615 0.84 -1.8 1,587,460 - MMJJ_RS08615 0.85 +1.1 1,587,465 - MMJJ_RS08615 0.86 -3.5 1,587,466 + MMJJ_RS08615 0.86 -3.5 1,587,471 + MMJJ_RS08615 0.86 -1.8 1,587,514 + -1.5 1,587,659 + -3.3 1,587,671 + -2.1 1,587,886 - MMJJ_RS08620 0.20 -2.1 1,587,952 - MMJJ_RS08620 0.26 +0.3 1,588,008 + MMJJ_RS08620 0.31 -1.9 1,588,326 + MMJJ_RS08620 0.60 -0.7 1,588,402 - -1.7 1,588,408 - MMJJ_RS08620 0.67 -2.1 1,588,445 + MMJJ_RS08620 0.71 -1.7 1,588,475 - MMJJ_RS08620 0.73 +0.1 1,588,476 + MMJJ_RS08620 0.74 -2.6 1,588,528 - MMJJ_RS08620 0.78 -3.8 1,588,540 - MMJJ_RS08620 0.79 -2.8 1,588,653 + +0.1 1,588,681 + -1.2 1,588,681 + -0.7 1,588,688 + -2.5 1,588,688 + +2.1 1,588,696 - -1.7 1,588,697 + -1.7 1,588,697 + -3.6 1,588,697 + +1.7 1,588,697 + -1.1 1,588,697 + -0.9 1,588,705 - -1.2 1,588,705 - -2.1 1,588,706 + -3.8 1,588,706 + -1.5 1,588,767 - -2.0 1,588,838 - -2.9 1,588,839 + -2.2 1,588,839 + -1.9 1,588,843 + -0.1 1,588,857 - -2.7 1,588,874 + -3.2 1,588,916 - MMJJ_RS08625 0.14 -2.8 1,588,986 - MMJJ_RS08625 0.22 -3.2 1,588,986 - MMJJ_RS08625 0.22 -1.7 1,588,987 + MMJJ_RS08625 0.22 -1.9 1,588,990 - MMJJ_RS08625 0.22 -0.9 1,588,990 - MMJJ_RS08625 0.22 -0.5 1,588,994 + MMJJ_RS08625 0.23 -2.8 1,588,995 - MMJJ_RS08625 0.23 -2.5 1,588,996 + MMJJ_RS08625 0.23 -0.9 1,589,001 - MMJJ_RS08625 0.23 -4.4 1,589,040 - MMJJ_RS08625 0.27 -4.2 1,589,060 - MMJJ_RS08625 0.29 -1.9 1,589,229 + MMJJ_RS08625 0.47 -1.5 1,589,255 - MMJJ_RS08625 0.50 -1.9 1,589,306 - MMJJ_RS08625 0.55 -2.4 1,589,346 - MMJJ_RS08625 0.60 -2.8 1,589,346 - MMJJ_RS08625 0.60 -3.1 1,589,346 - MMJJ_RS08625 0.60 -1.9 1,589,346 - MMJJ_RS08625 0.60 +1.6 1,589,348 - MMJJ_RS08625 0.60 -2.6 1,589,364 - MMJJ_RS08625 0.61 -1.2 1,589,365 + MMJJ_RS08625 0.62 -1.2 1,589,412 - MMJJ_RS08625 0.66 -1.5 1,589,412 - MMJJ_RS08625 0.66 -3.1 1,589,413 + MMJJ_RS08625 0.67 -3.3 1,589,413 + -0.5 1,589,432 - MMJJ_RS08625 0.69 -3.2 1,589,437 - MMJJ_RS08625 0.69 -2.1 1,589,474 + MMJJ_RS08625 0.73 -0.5 1,589,673 - -2.9
Or see this region's nucleotide sequence