Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS08620

Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS08615 and MMJJ_RS08620 are separated by 28 nucleotidesMMJJ_RS08620 and MMJJ_RS08625 are separated by 10 nucleotidesMMJJ_RS08625 and MMJJ_RS08630 are separated by 13 nucleotides MMJJ_RS08615: MMJJ_RS08615 - oligosaccharide repeat unit polymerase family protein, at 1,586,456 to 1,587,631 _RS08615 MMJJ_RS08620: MMJJ_RS08620 - DUF373 family protein, at 1,587,660 to 1,588,769 _RS08620 MMJJ_RS08625: MMJJ_RS08625 - magnesium/cobalt transporter CorA, at 1,588,780 to 1,589,730 _RS08625 MMJJ_RS08630: MMJJ_RS08630 - signal recognition particle subunit SRP19/SEC65 family protein, at 1,589,744 to 1,590,013 _RS08630 Position (kb) 1587 1588 1589Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1586.691 kb on - strand, within MMJJ_RS08615at 1586.775 kb on - strand, within MMJJ_RS08615at 1586.805 kb on - strand, within MMJJ_RS08615at 1586.806 kb on + strand, within MMJJ_RS08615at 1586.838 kb on + strand, within MMJJ_RS08615at 1586.871 kb on - strand, within MMJJ_RS08615at 1586.881 kb on + strand, within MMJJ_RS08615at 1586.881 kb on + strand, within MMJJ_RS08615at 1586.905 kb on + strand, within MMJJ_RS08615at 1586.905 kb on + strand, within MMJJ_RS08615at 1586.967 kb on - strand, within MMJJ_RS08615at 1587.020 kb on - strand, within MMJJ_RS08615at 1587.031 kb on + strand, within MMJJ_RS08615at 1587.081 kb on - strand, within MMJJ_RS08615at 1587.082 kb on + strand, within MMJJ_RS08615at 1587.103 kb on - strand, within MMJJ_RS08615at 1587.153 kb on - strand, within MMJJ_RS08615at 1587.154 kb on + strand, within MMJJ_RS08615at 1587.157 kb on - strandat 1587.273 kb on - strand, within MMJJ_RS08615at 1587.274 kb on + strand, within MMJJ_RS08615at 1587.283 kb on - strand, within MMJJ_RS08615at 1587.286 kb on + strand, within MMJJ_RS08615at 1587.323 kb on + strand, within MMJJ_RS08615at 1587.354 kb on - strand, within MMJJ_RS08615at 1587.354 kb on - strand, within MMJJ_RS08615at 1587.373 kb on - strand, within MMJJ_RS08615at 1587.374 kb on + strand, within MMJJ_RS08615at 1587.374 kb on + strand, within MMJJ_RS08615at 1587.374 kb on + strand, within MMJJ_RS08615at 1587.376 kb on - strand, within MMJJ_RS08615at 1587.377 kb on + strand, within MMJJ_RS08615at 1587.380 kb on + strand, within MMJJ_RS08615at 1587.405 kb on + strand, within MMJJ_RS08615at 1587.429 kb on - strand, within MMJJ_RS08615at 1587.445 kb on + strand, within MMJJ_RS08615at 1587.445 kb on + strand, within MMJJ_RS08615at 1587.460 kb on - strand, within MMJJ_RS08615at 1587.465 kb on - strand, within MMJJ_RS08615at 1587.466 kb on + strand, within MMJJ_RS08615at 1587.471 kb on + strand, within MMJJ_RS08615at 1587.514 kb on + strandat 1587.659 kb on + strandat 1587.671 kb on + strandat 1587.886 kb on - strand, within MMJJ_RS08620at 1587.952 kb on - strand, within MMJJ_RS08620at 1588.008 kb on + strand, within MMJJ_RS08620at 1588.326 kb on + strand, within MMJJ_RS08620at 1588.402 kb on - strandat 1588.408 kb on - strand, within MMJJ_RS08620at 1588.445 kb on + strand, within MMJJ_RS08620at 1588.475 kb on - strand, within MMJJ_RS08620at 1588.476 kb on + strand, within MMJJ_RS08620at 1588.528 kb on - strand, within MMJJ_RS08620at 1588.540 kb on - strand, within MMJJ_RS08620at 1588.653 kb on + strandat 1588.681 kb on + strandat 1588.681 kb on + strandat 1588.688 kb on + strandat 1588.688 kb on + strandat 1588.696 kb on - strandat 1588.697 kb on + strandat 1588.697 kb on + strandat 1588.697 kb on + strandat 1588.697 kb on + strandat 1588.697 kb on + strandat 1588.705 kb on - strandat 1588.705 kb on - strandat 1588.706 kb on + strandat 1588.706 kb on + strandat 1588.767 kb on - strandat 1588.838 kb on - strandat 1588.839 kb on + strandat 1588.839 kb on + strandat 1588.843 kb on + strandat 1588.857 kb on - strandat 1588.874 kb on + strandat 1588.916 kb on - strand, within MMJJ_RS08625at 1588.986 kb on - strand, within MMJJ_RS08625at 1588.986 kb on - strand, within MMJJ_RS08625at 1588.987 kb on + strand, within MMJJ_RS08625at 1588.990 kb on - strand, within MMJJ_RS08625at 1588.990 kb on - strand, within MMJJ_RS08625at 1588.994 kb on + strand, within MMJJ_RS08625at 1588.995 kb on - strand, within MMJJ_RS08625at 1588.996 kb on + strand, within MMJJ_RS08625at 1589.001 kb on - strand, within MMJJ_RS08625at 1589.040 kb on - strand, within MMJJ_RS08625at 1589.060 kb on - strand, within MMJJ_RS08625at 1589.229 kb on + strand, within MMJJ_RS08625at 1589.255 kb on - strand, within MMJJ_RS08625at 1589.306 kb on - strand, within MMJJ_RS08625at 1589.346 kb on - strand, within MMJJ_RS08625at 1589.346 kb on - strand, within MMJJ_RS08625at 1589.346 kb on - strand, within MMJJ_RS08625at 1589.346 kb on - strand, within MMJJ_RS08625at 1589.348 kb on - strand, within MMJJ_RS08625at 1589.364 kb on - strand, within MMJJ_RS08625at 1589.365 kb on + strand, within MMJJ_RS08625at 1589.412 kb on - strand, within MMJJ_RS08625at 1589.412 kb on - strand, within MMJJ_RS08625at 1589.413 kb on + strand, within MMJJ_RS08625at 1589.413 kb on + strandat 1589.432 kb on - strand, within MMJJ_RS08625at 1589.437 kb on - strand, within MMJJ_RS08625at 1589.474 kb on + strand, within MMJJ_RS08625at 1589.673 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride
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1,586,691 - MMJJ_RS08615 0.20 +0.3
1,586,775 - MMJJ_RS08615 0.27 -1.9
1,586,805 - MMJJ_RS08615 0.30 -1.0
1,586,806 + MMJJ_RS08615 0.30 -1.6
1,586,838 + MMJJ_RS08615 0.32 +0.8
1,586,871 - MMJJ_RS08615 0.35 -1.4
1,586,881 + MMJJ_RS08615 0.36 -0.9
1,586,881 + MMJJ_RS08615 0.36 +0.9
1,586,905 + MMJJ_RS08615 0.38 -0.0
1,586,905 + MMJJ_RS08615 0.38 +0.3
1,586,967 - MMJJ_RS08615 0.43 -0.1
1,587,020 - MMJJ_RS08615 0.48 -0.2
1,587,031 + MMJJ_RS08615 0.49 -1.7
1,587,081 - MMJJ_RS08615 0.53 -0.9
1,587,082 + MMJJ_RS08615 0.53 -0.1
1,587,103 - MMJJ_RS08615 0.55 +0.4
1,587,153 - MMJJ_RS08615 0.59 +0.5
1,587,154 + MMJJ_RS08615 0.59 -0.4
1,587,157 - +0.1
1,587,273 - MMJJ_RS08615 0.69 -1.8
1,587,274 + MMJJ_RS08615 0.70 +0.5
1,587,283 - MMJJ_RS08615 0.70 -1.0
1,587,286 + MMJJ_RS08615 0.71 -0.1
1,587,323 + MMJJ_RS08615 0.74 -1.7
1,587,354 - MMJJ_RS08615 0.76 -1.1
1,587,354 - MMJJ_RS08615 0.76 -2.5
1,587,373 - MMJJ_RS08615 0.78 -0.1
1,587,374 + MMJJ_RS08615 0.78 -0.8
1,587,374 + MMJJ_RS08615 0.78 -1.7
1,587,374 + MMJJ_RS08615 0.78 +0.8
1,587,376 - MMJJ_RS08615 0.78 -0.2
1,587,377 + MMJJ_RS08615 0.78 -0.4
1,587,380 + MMJJ_RS08615 0.79 -1.7
1,587,405 + MMJJ_RS08615 0.81 -2.4
1,587,429 - MMJJ_RS08615 0.83 -0.4
1,587,445 + MMJJ_RS08615 0.84 -2.7
1,587,445 + MMJJ_RS08615 0.84 -1.8
1,587,460 - MMJJ_RS08615 0.85 +1.1
1,587,465 - MMJJ_RS08615 0.86 -3.5
1,587,466 + MMJJ_RS08615 0.86 -3.5
1,587,471 + MMJJ_RS08615 0.86 -1.8
1,587,514 + -1.5
1,587,659 + -3.3
1,587,671 + -2.1
1,587,886 - MMJJ_RS08620 0.20 -2.1
1,587,952 - MMJJ_RS08620 0.26 +0.3
1,588,008 + MMJJ_RS08620 0.31 -1.9
1,588,326 + MMJJ_RS08620 0.60 -0.7
1,588,402 - -1.7
1,588,408 - MMJJ_RS08620 0.67 -2.1
1,588,445 + MMJJ_RS08620 0.71 -1.7
1,588,475 - MMJJ_RS08620 0.73 +0.1
1,588,476 + MMJJ_RS08620 0.74 -2.6
1,588,528 - MMJJ_RS08620 0.78 -3.8
1,588,540 - MMJJ_RS08620 0.79 -2.8
1,588,653 + +0.1
1,588,681 + -1.2
1,588,681 + -0.7
1,588,688 + -2.5
1,588,688 + +2.1
1,588,696 - -1.7
1,588,697 + -1.7
1,588,697 + -3.6
1,588,697 + +1.7
1,588,697 + -1.1
1,588,697 + -0.9
1,588,705 - -1.2
1,588,705 - -2.1
1,588,706 + -3.8
1,588,706 + -1.5
1,588,767 - -2.0
1,588,838 - -2.9
1,588,839 + -2.2
1,588,839 + -1.9
1,588,843 + -0.1
1,588,857 - -2.7
1,588,874 + -3.2
1,588,916 - MMJJ_RS08625 0.14 -2.8
1,588,986 - MMJJ_RS08625 0.22 -3.2
1,588,986 - MMJJ_RS08625 0.22 -1.7
1,588,987 + MMJJ_RS08625 0.22 -1.9
1,588,990 - MMJJ_RS08625 0.22 -0.9
1,588,990 - MMJJ_RS08625 0.22 -0.5
1,588,994 + MMJJ_RS08625 0.23 -2.8
1,588,995 - MMJJ_RS08625 0.23 -2.5
1,588,996 + MMJJ_RS08625 0.23 -0.9
1,589,001 - MMJJ_RS08625 0.23 -4.4
1,589,040 - MMJJ_RS08625 0.27 -4.2
1,589,060 - MMJJ_RS08625 0.29 -1.9
1,589,229 + MMJJ_RS08625 0.47 -1.5
1,589,255 - MMJJ_RS08625 0.50 -1.9
1,589,306 - MMJJ_RS08625 0.55 -2.4
1,589,346 - MMJJ_RS08625 0.60 -2.8
1,589,346 - MMJJ_RS08625 0.60 -3.1
1,589,346 - MMJJ_RS08625 0.60 -1.9
1,589,346 - MMJJ_RS08625 0.60 +1.6
1,589,348 - MMJJ_RS08625 0.60 -2.6
1,589,364 - MMJJ_RS08625 0.61 -1.2
1,589,365 + MMJJ_RS08625 0.62 -1.2
1,589,412 - MMJJ_RS08625 0.66 -1.5
1,589,412 - MMJJ_RS08625 0.66 -3.1
1,589,413 + MMJJ_RS08625 0.67 -3.3
1,589,413 + -0.5
1,589,432 - MMJJ_RS08625 0.69 -3.2
1,589,437 - MMJJ_RS08625 0.69 -2.1
1,589,474 + MMJJ_RS08625 0.73 -0.5
1,589,673 - -2.9

Or see this region's nucleotide sequence