Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08020 and MMJJ_RS08025 are separated by 72 nucleotides MMJJ_RS08025 and MMJJ_RS08030 are separated by 94 nucleotides MMJJ_RS08030 and MMJJ_RS08035 are separated by 13 nucleotides MMJJ_RS08035 and MMJJ_RS08040 are separated by 81 nucleotides
MMJJ_RS08020: MMJJ_RS08020 - nucleotidyltransferase domain-containing protein, at 1,468,595 to 1,469,653
_RS08020
MMJJ_RS08025: MMJJ_RS08025 - hypothetical protein, at 1,469,726 to 1,470,028
_RS08025
MMJJ_RS08030: MMJJ_RS08030 - hypothetical protein, at 1,470,123 to 1,470,770
_RS08030
MMJJ_RS08035: MMJJ_RS08035 - cobalt-precorrin-7 (C(5))-methyltransferase, at 1,470,784 to 1,471,443
_RS08035
MMJJ_RS08040: MMJJ_RS08040 - NAD(P)/FAD-dependent oxidoreductase, at 1,471,525 to 1,472,682
_RS08040
Position (kb)
1470
1471 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1469.192 kb on - strand, within MMJJ_RS08020 at 1469.257 kb on - strand, within MMJJ_RS08020 at 1469.258 kb on + strand, within MMJJ_RS08020 at 1469.397 kb on - strand, within MMJJ_RS08020 at 1469.447 kb on + strand, within MMJJ_RS08020 at 1469.449 kb on - strand, within MMJJ_RS08020 at 1469.484 kb on + strand, within MMJJ_RS08020 at 1469.489 kb on - strand, within MMJJ_RS08020 at 1469.549 kb on - strand at 1469.549 kb on - strand at 1469.550 kb on + strand at 1469.642 kb on + strand at 1469.682 kb on + strand at 1469.689 kb on - strand at 1469.689 kb on - strand at 1469.690 kb on + strand at 1469.694 kb on - strand at 1469.695 kb on + strand at 1469.696 kb on - strand at 1469.696 kb on - strand at 1469.696 kb on - strand at 1469.697 kb on + strand at 1469.702 kb on + strand at 1469.706 kb on - strand at 1469.706 kb on - strand at 1469.706 kb on - strand at 1469.706 kb on - strand at 1469.707 kb on + strand at 1469.710 kb on - strand at 1469.710 kb on - strand at 1469.710 kb on - strand at 1469.710 kb on - strand at 1469.711 kb on + strand at 1469.711 kb on + strand at 1469.711 kb on + strand at 1469.711 kb on + strand at 1469.711 kb on + strand at 1469.711 kb on + strand at 1469.712 kb on - strand at 1469.713 kb on + strand at 1469.713 kb on + strand at 1469.713 kb on + strand at 1469.835 kb on + strand, within MMJJ_RS08025 at 1469.835 kb on + strand, within MMJJ_RS08025 at 1469.912 kb on - strand, within MMJJ_RS08025 at 1469.913 kb on + strand, within MMJJ_RS08025 at 1469.974 kb on - strand, within MMJJ_RS08025 at 1469.975 kb on + strand, within MMJJ_RS08025 at 1469.987 kb on + strand, within MMJJ_RS08025 at 1470.020 kb on - strand at 1470.033 kb on - strand at 1470.055 kb on + strand at 1470.060 kb on - strand at 1470.063 kb on - strand at 1470.064 kb on + strand at 1470.068 kb on + strand at 1470.161 kb on - strand at 1470.164 kb on - strand at 1470.243 kb on - strand, within MMJJ_RS08030 at 1470.362 kb on + strand, within MMJJ_RS08030 at 1470.391 kb on - strand, within MMJJ_RS08030 at 1470.409 kb on - strand, within MMJJ_RS08030 at 1470.409 kb on - strand, within MMJJ_RS08030 at 1470.472 kb on + strand, within MMJJ_RS08030 at 1470.473 kb on - strand, within MMJJ_RS08030 at 1470.539 kb on - strand, within MMJJ_RS08030 at 1470.540 kb on + strand, within MMJJ_RS08030 at 1470.545 kb on - strand, within MMJJ_RS08030 at 1470.570 kb on - strand, within MMJJ_RS08030 at 1470.571 kb on + strand, within MMJJ_RS08030 at 1470.581 kb on + strand, within MMJJ_RS08030 at 1470.608 kb on - strand, within MMJJ_RS08030 at 1470.609 kb on + strand, within MMJJ_RS08030 at 1470.614 kb on - strand, within MMJJ_RS08030 at 1470.615 kb on + strand, within MMJJ_RS08030 at 1470.618 kb on - strand, within MMJJ_RS08030 at 1470.618 kb on - strand, within MMJJ_RS08030 at 1470.619 kb on + strand, within MMJJ_RS08030 at 1470.625 kb on + strand, within MMJJ_RS08030 at 1470.630 kb on + strand, within MMJJ_RS08030 at 1470.630 kb on + strand, within MMJJ_RS08030 at 1470.635 kb on - strand, within MMJJ_RS08030 at 1470.643 kb on - strand, within MMJJ_RS08030 at 1470.643 kb on - strand, within MMJJ_RS08030 at 1470.644 kb on + strand, within MMJJ_RS08030 at 1470.648 kb on + strand, within MMJJ_RS08030 at 1470.665 kb on - strand, within MMJJ_RS08030 at 1470.723 kb on - strand at 1470.726 kb on - strand at 1470.726 kb on - strand at 1470.726 kb on - strand at 1470.727 kb on + strand at 1470.732 kb on - strand at 1470.750 kb on - strand at 1470.775 kb on + strand at 1470.791 kb on + strand at 1470.791 kb on + strand at 1470.791 kb on + strand at 1470.792 kb on - strand at 1470.793 kb on + strand at 1470.809 kb on - strand at 1471.027 kb on - strand, within MMJJ_RS08035 at 1471.027 kb on - strand, within MMJJ_RS08035 at 1471.028 kb on + strand, within MMJJ_RS08035 at 1471.042 kb on + strand, within MMJJ_RS08035 at 1471.043 kb on - strand, within MMJJ_RS08035 at 1471.146 kb on - strand, within MMJJ_RS08035 at 1471.146 kb on - strand at 1471.146 kb on - strand, within MMJJ_RS08035 at 1471.146 kb on - strand, within MMJJ_RS08035 at 1471.154 kb on + strand, within MMJJ_RS08035 at 1471.242 kb on + strand, within MMJJ_RS08035 at 1471.262 kb on + strand, within MMJJ_RS08035 at 1471.365 kb on - strand, within MMJJ_RS08035 at 1471.370 kb on - strand, within MMJJ_RS08035 at 1471.370 kb on - strand, within MMJJ_RS08035 at 1471.371 kb on + strand, within MMJJ_RS08035 at 1471.371 kb on + strand, within MMJJ_RS08035 at 1471.401 kb on - strand at 1471.403 kb on - strand at 1471.403 kb on - strand at 1471.404 kb on + strand at 1471.413 kb on - strand at 1471.454 kb on - strand at 1471.455 kb on + strand at 1471.455 kb on + strand at 1471.455 kb on + strand at 1471.460 kb on - strand at 1471.461 kb on + strand at 1471.472 kb on - strand at 1471.473 kb on + strand at 1471.473 kb on + strand at 1471.473 kb on + strand at 1471.476 kb on + strand at 1471.476 kb on + strand at 1471.477 kb on - strand at 1471.477 kb on - strand at 1471.477 kb on - strand at 1471.478 kb on + strand at 1471.478 kb on + strand at 1471.485 kb on + strand at 1471.492 kb on + strand at 1471.492 kb on + strand at 1471.521 kb on - strand at 1471.522 kb on + strand at 1471.540 kb on - strand at 1471.541 kb on + strand at 1471.541 kb on + strand at 1471.541 kb on + strand at 1471.550 kb on - strand at 1471.550 kb on - strand at 1471.578 kb on - strand at 1471.578 kb on - strand at 1471.579 kb on + strand at 1471.593 kb on - strand at 1471.602 kb on - strand at 1471.667 kb on - strand, within MMJJ_RS08040 at 1471.668 kb on + strand, within MMJJ_RS08040 at 1471.716 kb on - strand, within MMJJ_RS08040 at 1471.723 kb on + strand, within MMJJ_RS08040 at 1471.735 kb on + strand, within MMJJ_RS08040
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,469,192 - MMJJ_RS08020 0.56 -4.3 1,469,257 - MMJJ_RS08020 0.63 -2.6 1,469,258 + MMJJ_RS08020 0.63 -3.2 1,469,397 - MMJJ_RS08020 0.76 -2.3 1,469,447 + MMJJ_RS08020 0.80 -1.2 1,469,449 - MMJJ_RS08020 0.81 -3.9 1,469,484 + MMJJ_RS08020 0.84 -2.9 1,469,489 - MMJJ_RS08020 0.84 -3.6 1,469,549 - -2.6 1,469,549 - -2.2 1,469,550 + -3.8 1,469,642 + -2.4 1,469,682 + +0.2 1,469,689 - +0.9 1,469,689 - +0.3 1,469,690 + -0.2 1,469,694 - -0.1 1,469,695 + +0.2 1,469,696 - -0.8 1,469,696 - -1.8 1,469,696 - -1.4 1,469,697 + -2.2 1,469,702 + +0.1 1,469,706 - +1.0 1,469,706 - -0.1 1,469,706 - +0.2 1,469,706 - -0.5 1,469,707 + +0.3 1,469,710 - -0.7 1,469,710 - +0.4 1,469,710 - +0.3 1,469,710 - -1.8 1,469,711 + -0.5 1,469,711 + +0.9 1,469,711 + +0.6 1,469,711 + -0.3 1,469,711 + -1.1 1,469,711 + -0.2 1,469,712 - +0.5 1,469,713 + -0.2 1,469,713 + +1.3 1,469,713 + +0.3 1,469,835 + MMJJ_RS08025 0.36 -2.0 1,469,835 + MMJJ_RS08025 0.36 -1.6 1,469,912 - MMJJ_RS08025 0.61 -0.2 1,469,913 + MMJJ_RS08025 0.62 -1.0 1,469,974 - MMJJ_RS08025 0.82 +0.9 1,469,975 + MMJJ_RS08025 0.82 +0.7 1,469,987 + MMJJ_RS08025 0.86 +0.5 1,470,020 - +0.7 1,470,033 - -0.8 1,470,055 + -0.1 1,470,060 - +0.6 1,470,063 - +0.3 1,470,064 + -0.5 1,470,068 + -0.8 1,470,161 - -0.2 1,470,164 - +1.1 1,470,243 - MMJJ_RS08030 0.19 +0.7 1,470,362 + MMJJ_RS08030 0.37 +0.7 1,470,391 - MMJJ_RS08030 0.41 -0.3 1,470,409 - MMJJ_RS08030 0.44 -0.5 1,470,409 - MMJJ_RS08030 0.44 +0.4 1,470,472 + MMJJ_RS08030 0.54 +0.2 1,470,473 - MMJJ_RS08030 0.54 -0.8 1,470,539 - MMJJ_RS08030 0.64 +0.3 1,470,540 + MMJJ_RS08030 0.64 +1.1 1,470,545 - MMJJ_RS08030 0.65 +0.6 1,470,570 - MMJJ_RS08030 0.69 +0.8 1,470,571 + MMJJ_RS08030 0.69 +0.8 1,470,581 + MMJJ_RS08030 0.71 -0.6 1,470,608 - MMJJ_RS08030 0.75 +0.4 1,470,609 + MMJJ_RS08030 0.75 -0.2 1,470,614 - MMJJ_RS08030 0.76 +0.8 1,470,615 + MMJJ_RS08030 0.76 -0.4 1,470,618 - MMJJ_RS08030 0.76 -1.8 1,470,618 - MMJJ_RS08030 0.76 +0.4 1,470,619 + MMJJ_RS08030 0.77 +0.1 1,470,625 + MMJJ_RS08030 0.77 +0.2 1,470,630 + MMJJ_RS08030 0.78 +0.0 1,470,630 + MMJJ_RS08030 0.78 -1.2 1,470,635 - MMJJ_RS08030 0.79 +1.4 1,470,643 - MMJJ_RS08030 0.80 +1.3 1,470,643 - MMJJ_RS08030 0.80 +0.7 1,470,644 + MMJJ_RS08030 0.80 +0.0 1,470,648 + MMJJ_RS08030 0.81 +1.1 1,470,665 - MMJJ_RS08030 0.84 +0.5 1,470,723 - -0.7 1,470,726 - +0.5 1,470,726 - +0.8 1,470,726 - -0.8 1,470,727 + -0.5 1,470,732 - -0.4 1,470,750 - +0.7 1,470,775 + +0.3 1,470,791 + -2.7 1,470,791 + -0.4 1,470,791 + -0.4 1,470,792 - +0.6 1,470,793 + -0.5 1,470,809 - -0.7 1,471,027 - MMJJ_RS08035 0.37 -3.3 1,471,027 - MMJJ_RS08035 0.37 -2.9 1,471,028 + MMJJ_RS08035 0.37 -3.4 1,471,042 + MMJJ_RS08035 0.39 -3.2 1,471,043 - MMJJ_RS08035 0.39 -3.9 1,471,146 - MMJJ_RS08035 0.55 -2.7 1,471,146 - -0.4 1,471,146 - MMJJ_RS08035 0.55 -2.8 1,471,146 - MMJJ_RS08035 0.55 -3.8 1,471,154 + MMJJ_RS08035 0.56 -3.9 1,471,242 + MMJJ_RS08035 0.69 -2.2 1,471,262 + MMJJ_RS08035 0.72 -3.6 1,471,365 - MMJJ_RS08035 0.88 -2.4 1,471,370 - MMJJ_RS08035 0.89 -4.0 1,471,370 - MMJJ_RS08035 0.89 -3.3 1,471,371 + MMJJ_RS08035 0.89 -2.8 1,471,371 + MMJJ_RS08035 0.89 -2.6 1,471,401 - -2.6 1,471,403 - -0.8 1,471,403 - -1.9 1,471,404 + -3.5 1,471,413 - -3.4 1,471,454 - -0.4 1,471,455 + -0.2 1,471,455 + +0.5 1,471,455 + +0.5 1,471,460 - -0.1 1,471,461 + -0.2 1,471,472 - -1.7 1,471,473 + -0.8 1,471,473 + -0.9 1,471,473 + -0.4 1,471,476 + -0.1 1,471,476 + +0.5 1,471,477 - -0.6 1,471,477 - -0.5 1,471,477 - +0.1 1,471,478 + -0.1 1,471,478 + +0.3 1,471,485 + -1.0 1,471,492 + +0.1 1,471,492 + -0.4 1,471,521 - -1.3 1,471,522 + -0.2 1,471,540 - -0.7 1,471,541 + +0.4 1,471,541 + +0.1 1,471,541 + -0.1 1,471,550 - +0.2 1,471,550 - -0.2 1,471,578 - -0.1 1,471,578 - +0.6 1,471,579 + +0.1 1,471,593 - +0.8 1,471,602 - -0.8 1,471,667 - MMJJ_RS08040 0.12 +0.5 1,471,668 + MMJJ_RS08040 0.12 +0.5 1,471,716 - MMJJ_RS08040 0.16 +0.1 1,471,723 + MMJJ_RS08040 0.17 -1.8 1,471,735 + MMJJ_RS08040 0.18 -1.7
Or see this region's nucleotide sequence