Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08000 and MMJJ_RS08005 are separated by 79 nucleotides MMJJ_RS08005 and MMJJ_RS08010 are separated by 50 nucleotides MMJJ_RS08010 and MMJJ_RS08015 are separated by 20 nucleotides MMJJ_RS08015 and MMJJ_RS08020 are separated by 28 nucleotides
MMJJ_RS08000: MMJJ_RS08000 - HesA/MoeB/ThiF family protein, at 1,465,655 to 1,466,374
_RS08000
MMJJ_RS08005: MMJJ_RS08005 - formate dehydrogenase accessory sulfurtransferase FdhD, at 1,466,454 to 1,467,155
_RS08005
MMJJ_RS08010: MMJJ_RS08010 - phosphoadenosine phosphosulfate reductase family protein, at 1,467,206 to 1,468,096
_RS08010
MMJJ_RS08015: MMJJ_RS08015 - HAD family hydrolase, at 1,468,117 to 1,468,566
_RS08015
MMJJ_RS08020: MMJJ_RS08020 - nucleotidyltransferase domain-containing protein, at 1,468,595 to 1,469,653
_RS08020
Position (kb)
1467
1468
1469 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1466.244 kb on + strand, within MMJJ_RS08000 at 1466.291 kb on - strand, within MMJJ_RS08000 at 1466.342 kb on - strand at 1466.358 kb on - strand at 1466.359 kb on + strand at 1466.359 kb on + strand at 1466.375 kb on + strand at 1466.375 kb on + strand at 1466.377 kb on - strand at 1466.386 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.400 kb on - strand at 1466.407 kb on - strand at 1466.407 kb on - strand at 1466.407 kb on - strand at 1466.407 kb on - strand at 1466.413 kb on + strand at 1466.414 kb on - strand at 1466.414 kb on - strand at 1466.414 kb on - strand at 1466.415 kb on + strand at 1466.415 kb on + strand at 1466.418 kb on + strand at 1466.418 kb on + strand at 1466.420 kb on - strand at 1466.420 kb on - strand at 1466.420 kb on - strand at 1466.429 kb on - strand at 1466.429 kb on - strand at 1466.430 kb on + strand at 1466.430 kb on + strand at 1466.460 kb on + strand at 1466.478 kb on + strand at 1466.484 kb on - strand at 1466.534 kb on + strand, within MMJJ_RS08005 at 1466.557 kb on + strand, within MMJJ_RS08005 at 1466.573 kb on + strand, within MMJJ_RS08005 at 1466.590 kb on - strand, within MMJJ_RS08005 at 1466.644 kb on + strand, within MMJJ_RS08005 at 1466.673 kb on + strand, within MMJJ_RS08005 at 1466.677 kb on - strand, within MMJJ_RS08005 at 1466.677 kb on - strand, within MMJJ_RS08005 at 1466.678 kb on + strand, within MMJJ_RS08005 at 1466.678 kb on + strand, within MMJJ_RS08005 at 1466.678 kb on + strand, within MMJJ_RS08005 at 1466.678 kb on + strand, within MMJJ_RS08005 at 1466.678 kb on + strand, within MMJJ_RS08005 at 1466.692 kb on - strand, within MMJJ_RS08005 at 1466.692 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.694 kb on - strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.695 kb on + strand, within MMJJ_RS08005 at 1466.698 kb on - strand, within MMJJ_RS08005 at 1466.698 kb on - strand, within MMJJ_RS08005 at 1466.698 kb on - strand, within MMJJ_RS08005 at 1466.699 kb on + strand, within MMJJ_RS08005 at 1466.722 kb on - strand, within MMJJ_RS08005 at 1466.760 kb on + strand, within MMJJ_RS08005 at 1466.766 kb on + strand, within MMJJ_RS08005 at 1466.789 kb on - strand, within MMJJ_RS08005 at 1466.863 kb on - strand, within MMJJ_RS08005 at 1466.863 kb on - strand, within MMJJ_RS08005 at 1466.899 kb on - strand, within MMJJ_RS08005 at 1466.900 kb on + strand, within MMJJ_RS08005 at 1466.903 kb on - strand, within MMJJ_RS08005 at 1466.908 kb on - strand, within MMJJ_RS08005 at 1466.927 kb on + strand, within MMJJ_RS08005 at 1466.934 kb on + strand, within MMJJ_RS08005 at 1466.998 kb on - strand, within MMJJ_RS08005 at 1467.002 kb on + strand, within MMJJ_RS08005 at 1467.002 kb on + strand, within MMJJ_RS08005 at 1467.023 kb on + strand, within MMJJ_RS08005 at 1467.082 kb on - strand, within MMJJ_RS08005 at 1467.121 kb on + strand at 1467.139 kb on - strand at 1467.140 kb on + strand at 1467.288 kb on - strand at 1467.333 kb on - strand, within MMJJ_RS08010 at 1467.440 kb on + strand, within MMJJ_RS08010 at 1467.478 kb on - strand, within MMJJ_RS08010 at 1467.478 kb on - strand, within MMJJ_RS08010 at 1467.492 kb on - strand, within MMJJ_RS08010 at 1467.502 kb on - strand, within MMJJ_RS08010 at 1467.571 kb on - strand, within MMJJ_RS08010 at 1467.573 kb on - strand, within MMJJ_RS08010 at 1467.591 kb on - strand, within MMJJ_RS08010 at 1467.592 kb on + strand, within MMJJ_RS08010 at 1467.592 kb on + strand, within MMJJ_RS08010 at 1467.621 kb on - strand, within MMJJ_RS08010 at 1467.675 kb on + strand, within MMJJ_RS08010 at 1467.675 kb on + strand, within MMJJ_RS08010 at 1467.810 kb on - strand, within MMJJ_RS08010 at 1467.837 kb on - strand, within MMJJ_RS08010 at 1467.838 kb on + strand, within MMJJ_RS08010 at 1467.838 kb on + strand, within MMJJ_RS08010 at 1467.841 kb on - strand, within MMJJ_RS08010 at 1467.843 kb on - strand, within MMJJ_RS08010 at 1467.843 kb on - strand, within MMJJ_RS08010 at 1467.844 kb on + strand, within MMJJ_RS08010 at 1467.951 kb on - strand, within MMJJ_RS08010 at 1468.039 kb on + strand at 1468.138 kb on + strand at 1468.205 kb on + strand, within MMJJ_RS08015 at 1468.243 kb on - strand, within MMJJ_RS08015 at 1468.243 kb on - strand, within MMJJ_RS08015 at 1468.244 kb on + strand, within MMJJ_RS08015 at 1468.307 kb on - strand, within MMJJ_RS08015 at 1468.308 kb on + strand, within MMJJ_RS08015 at 1468.308 kb on + strand, within MMJJ_RS08015 at 1468.308 kb on + strand, within MMJJ_RS08015 at 1468.406 kb on - strand, within MMJJ_RS08015 at 1468.406 kb on - strand, within MMJJ_RS08015 at 1468.407 kb on + strand, within MMJJ_RS08015 at 1468.407 kb on + strand, within MMJJ_RS08015 at 1468.423 kb on - strand, within MMJJ_RS08015 at 1468.424 kb on + strand, within MMJJ_RS08015 at 1468.424 kb on + strand, within MMJJ_RS08015 at 1468.457 kb on + strand, within MMJJ_RS08015 at 1468.469 kb on + strand, within MMJJ_RS08015 at 1468.480 kb on - strand, within MMJJ_RS08015 at 1468.480 kb on - strand, within MMJJ_RS08015 at 1468.510 kb on - strand, within MMJJ_RS08015 at 1468.566 kb on - strand at 1468.601 kb on + strand at 1468.611 kb on + strand at 1468.615 kb on + strand at 1468.636 kb on - strand at 1468.702 kb on - strand, within MMJJ_RS08020 at 1468.703 kb on + strand, within MMJJ_RS08020 at 1468.703 kb on + strand, within MMJJ_RS08020 at 1468.718 kb on + strand, within MMJJ_RS08020 at 1468.718 kb on + strand, within MMJJ_RS08020 at 1468.719 kb on - strand, within MMJJ_RS08020 at 1468.719 kb on - strand, within MMJJ_RS08020 at 1468.719 kb on - strand, within MMJJ_RS08020 at 1468.719 kb on - strand, within MMJJ_RS08020 at 1468.720 kb on + strand, within MMJJ_RS08020 at 1468.720 kb on + strand, within MMJJ_RS08020 at 1468.734 kb on - strand, within MMJJ_RS08020 at 1468.734 kb on - strand, within MMJJ_RS08020 at 1469.025 kb on - strand, within MMJJ_RS08020 at 1469.026 kb on + strand, within MMJJ_RS08020 at 1469.030 kb on - strand, within MMJJ_RS08020 at 1469.031 kb on + strand, within MMJJ_RS08020 at 1469.031 kb on + strand, within MMJJ_RS08020 at 1469.031 kb on + strand, within MMJJ_RS08020 at 1469.054 kb on - strand, within MMJJ_RS08020 at 1469.054 kb on - strand, within MMJJ_RS08020 at 1469.058 kb on - strand, within MMJJ_RS08020 at 1469.058 kb on - strand, within MMJJ_RS08020 at 1469.094 kb on + strand, within MMJJ_RS08020
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,466,244 + MMJJ_RS08000 0.82 +3.3 1,466,291 - MMJJ_RS08000 0.88 -2.4 1,466,342 - -3.9 1,466,358 - -1.0 1,466,359 + -3.3 1,466,359 + -1.6 1,466,375 + -3.2 1,466,375 + -3.4 1,466,377 - -4.5 1,466,386 - -1.1 1,466,398 - +0.5 1,466,398 - -0.2 1,466,398 - -1.8 1,466,398 - +0.5 1,466,398 - -0.5 1,466,398 - -0.8 1,466,398 - +0.6 1,466,398 - +0.6 1,466,398 - +0.2 1,466,399 + +0.6 1,466,399 + +0.4 1,466,399 + -0.0 1,466,399 + -0.9 1,466,399 + -0.7 1,466,399 + +1.4 1,466,399 + +0.2 1,466,399 + -0.2 1,466,399 + -0.8 1,466,400 - -0.9 1,466,407 - +0.6 1,466,407 - -1.2 1,466,407 - +0.1 1,466,407 - +0.4 1,466,413 + -0.3 1,466,414 - +1.0 1,466,414 - +0.9 1,466,414 - -0.3 1,466,415 + -0.3 1,466,415 + -0.5 1,466,418 + +0.4 1,466,418 + +1.1 1,466,420 - -0.7 1,466,420 - +0.0 1,466,420 - -0.5 1,466,429 - +0.6 1,466,429 - -0.9 1,466,430 + -0.3 1,466,430 + +0.5 1,466,460 + +0.7 1,466,478 + -0.8 1,466,484 - -0.4 1,466,534 + MMJJ_RS08005 0.11 +0.5 1,466,557 + MMJJ_RS08005 0.15 +0.6 1,466,573 + MMJJ_RS08005 0.17 -0.1 1,466,590 - MMJJ_RS08005 0.19 -1.4 1,466,644 + MMJJ_RS08005 0.27 +0.7 1,466,673 + MMJJ_RS08005 0.31 +0.1 1,466,677 - MMJJ_RS08005 0.32 -0.9 1,466,677 - MMJJ_RS08005 0.32 -0.0 1,466,678 + MMJJ_RS08005 0.32 -1.4 1,466,678 + MMJJ_RS08005 0.32 -0.4 1,466,678 + MMJJ_RS08005 0.32 +0.3 1,466,678 + MMJJ_RS08005 0.32 -0.4 1,466,678 + MMJJ_RS08005 0.32 -0.1 1,466,692 - MMJJ_RS08005 0.34 -0.6 1,466,692 - MMJJ_RS08005 0.34 -0.7 1,466,694 - MMJJ_RS08005 0.34 -1.7 1,466,694 - MMJJ_RS08005 0.34 +0.6 1,466,694 - MMJJ_RS08005 0.34 +0.8 1,466,694 - MMJJ_RS08005 0.34 +0.6 1,466,694 - MMJJ_RS08005 0.34 -1.0 1,466,694 - MMJJ_RS08005 0.34 +0.2 1,466,694 - MMJJ_RS08005 0.34 -1.0 1,466,694 - MMJJ_RS08005 0.34 -0.4 1,466,694 - MMJJ_RS08005 0.34 +0.7 1,466,694 - MMJJ_RS08005 0.34 -2.4 1,466,694 - MMJJ_RS08005 0.34 -0.9 1,466,694 - MMJJ_RS08005 0.34 -0.2 1,466,694 - MMJJ_RS08005 0.34 -0.5 1,466,694 - MMJJ_RS08005 0.34 -0.5 1,466,695 + MMJJ_RS08005 0.34 -1.7 1,466,695 + MMJJ_RS08005 0.34 -0.3 1,466,695 + MMJJ_RS08005 0.34 +0.8 1,466,695 + MMJJ_RS08005 0.34 -0.3 1,466,695 + MMJJ_RS08005 0.34 -0.9 1,466,695 + MMJJ_RS08005 0.34 -1.0 1,466,695 + MMJJ_RS08005 0.34 -1.5 1,466,695 + MMJJ_RS08005 0.34 +0.9 1,466,695 + MMJJ_RS08005 0.34 -0.9 1,466,695 + MMJJ_RS08005 0.34 -0.8 1,466,695 + MMJJ_RS08005 0.34 -0.5 1,466,695 + MMJJ_RS08005 0.34 +0.8 1,466,698 - MMJJ_RS08005 0.35 -1.5 1,466,698 - MMJJ_RS08005 0.35 -0.0 1,466,698 - MMJJ_RS08005 0.35 -0.6 1,466,699 + MMJJ_RS08005 0.35 +0.2 1,466,722 - MMJJ_RS08005 0.38 +1.0 1,466,760 + MMJJ_RS08005 0.44 +0.6 1,466,766 + MMJJ_RS08005 0.44 +1.2 1,466,789 - MMJJ_RS08005 0.48 -0.4 1,466,863 - MMJJ_RS08005 0.58 +0.4 1,466,863 - MMJJ_RS08005 0.58 -1.4 1,466,899 - MMJJ_RS08005 0.63 -1.1 1,466,900 + MMJJ_RS08005 0.64 +0.2 1,466,903 - MMJJ_RS08005 0.64 -0.2 1,466,908 - MMJJ_RS08005 0.65 -1.6 1,466,927 + MMJJ_RS08005 0.67 +0.8 1,466,934 + MMJJ_RS08005 0.68 +0.1 1,466,998 - MMJJ_RS08005 0.77 -0.3 1,467,002 + MMJJ_RS08005 0.78 -0.9 1,467,002 + MMJJ_RS08005 0.78 -1.7 1,467,023 + MMJJ_RS08005 0.81 +1.1 1,467,082 - MMJJ_RS08005 0.89 -0.5 1,467,121 + -1.1 1,467,139 - +0.9 1,467,140 + +0.9 1,467,288 - -0.8 1,467,333 - MMJJ_RS08010 0.14 -2.7 1,467,440 + MMJJ_RS08010 0.26 -1.7 1,467,478 - MMJJ_RS08010 0.31 -2.0 1,467,478 - MMJJ_RS08010 0.31 -2.2 1,467,492 - MMJJ_RS08010 0.32 +1.2 1,467,502 - MMJJ_RS08010 0.33 -4.0 1,467,571 - MMJJ_RS08010 0.41 -2.8 1,467,573 - MMJJ_RS08010 0.41 -2.7 1,467,591 - MMJJ_RS08010 0.43 -2.2 1,467,592 + MMJJ_RS08010 0.43 -3.7 1,467,592 + MMJJ_RS08010 0.43 -2.4 1,467,621 - MMJJ_RS08010 0.47 -3.2 1,467,675 + MMJJ_RS08010 0.53 -1.2 1,467,675 + MMJJ_RS08010 0.53 +0.3 1,467,810 - MMJJ_RS08010 0.68 -1.3 1,467,837 - MMJJ_RS08010 0.71 -2.8 1,467,838 + MMJJ_RS08010 0.71 -2.3 1,467,838 + MMJJ_RS08010 0.71 -2.7 1,467,841 - MMJJ_RS08010 0.71 -1.0 1,467,843 - MMJJ_RS08010 0.71 -2.4 1,467,843 - MMJJ_RS08010 0.71 -2.5 1,467,844 + MMJJ_RS08010 0.72 -2.3 1,467,951 - MMJJ_RS08010 0.84 -1.3 1,468,039 + -1.2 1,468,138 + +1.0 1,468,205 + MMJJ_RS08015 0.20 +0.2 1,468,243 - MMJJ_RS08015 0.28 +0.2 1,468,243 - MMJJ_RS08015 0.28 -0.6 1,468,244 + MMJJ_RS08015 0.28 -0.1 1,468,307 - MMJJ_RS08015 0.42 -1.0 1,468,308 + MMJJ_RS08015 0.42 -0.2 1,468,308 + MMJJ_RS08015 0.42 -1.0 1,468,308 + MMJJ_RS08015 0.42 +1.1 1,468,406 - MMJJ_RS08015 0.64 +0.5 1,468,406 - MMJJ_RS08015 0.64 +0.3 1,468,407 + MMJJ_RS08015 0.64 +0.0 1,468,407 + MMJJ_RS08015 0.64 -0.2 1,468,423 - MMJJ_RS08015 0.68 +0.6 1,468,424 + MMJJ_RS08015 0.68 +1.2 1,468,424 + MMJJ_RS08015 0.68 +0.5 1,468,457 + MMJJ_RS08015 0.76 +0.1 1,468,469 + MMJJ_RS08015 0.78 -2.8 1,468,480 - MMJJ_RS08015 0.81 -1.4 1,468,480 - MMJJ_RS08015 0.81 +0.2 1,468,510 - MMJJ_RS08015 0.87 +0.8 1,468,566 - -0.1 1,468,601 + +0.4 1,468,611 + +0.3 1,468,615 + -2.3 1,468,636 - -1.3 1,468,702 - MMJJ_RS08020 0.10 -3.8 1,468,703 + MMJJ_RS08020 0.10 -2.9 1,468,703 + MMJJ_RS08020 0.10 -3.6 1,468,718 + MMJJ_RS08020 0.12 -4.4 1,468,718 + MMJJ_RS08020 0.12 -2.0 1,468,719 - MMJJ_RS08020 0.12 -0.1 1,468,719 - MMJJ_RS08020 0.12 -2.3 1,468,719 - MMJJ_RS08020 0.12 -2.4 1,468,719 - MMJJ_RS08020 0.12 -3.5 1,468,720 + MMJJ_RS08020 0.12 -2.3 1,468,720 + MMJJ_RS08020 0.12 -3.1 1,468,734 - MMJJ_RS08020 0.13 -2.3 1,468,734 - MMJJ_RS08020 0.13 -2.8 1,469,025 - MMJJ_RS08020 0.41 -2.6 1,469,026 + MMJJ_RS08020 0.41 -1.8 1,469,030 - MMJJ_RS08020 0.41 -1.8 1,469,031 + MMJJ_RS08020 0.41 -4.1 1,469,031 + MMJJ_RS08020 0.41 -2.0 1,469,031 + MMJJ_RS08020 0.41 -2.2 1,469,054 - MMJJ_RS08020 0.43 -3.8 1,469,054 - MMJJ_RS08020 0.43 -3.8 1,469,058 - MMJJ_RS08020 0.44 -2.1 1,469,058 - MMJJ_RS08020 0.44 -3.8 1,469,094 + MMJJ_RS08020 0.47 -2.5
Or see this region's nucleotide sequence