Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07945 and MMJJ_RS07950 are separated by 22 nucleotides MMJJ_RS07950 and MMJJ_RS07955 are separated by 203 nucleotides MMJJ_RS07955 and MMJJ_RS07960 are separated by 52 nucleotides
MMJJ_RS07945: MMJJ_RS07945 - 4Fe-4S binding protein, at 1,457,530 to 1,458,588
_RS07945
MMJJ_RS07950: MMJJ_RS07950 - 4Fe-4S binding protein, at 1,458,611 to 1,459,057
_RS07950
MMJJ_RS07955: MMJJ_RS07955 - ThiF family adenylyltransferase, at 1,459,261 to 1,459,875
_RS07955
MMJJ_RS07960: MMJJ_RS07960 - quinolinate synthase NadA, at 1,459,928 to 1,460,845
_RS07960
Position (kb)
1458
1459
1460 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1457.655 kb on - strand, within MMJJ_RS07945 at 1457.655 kb on - strand at 1457.660 kb on + strand, within MMJJ_RS07945 at 1457.682 kb on - strand at 1457.686 kb on - strand, within MMJJ_RS07945 at 1458.002 kb on - strand, within MMJJ_RS07945 at 1458.016 kb on - strand, within MMJJ_RS07945 at 1458.053 kb on + strand at 1458.122 kb on + strand, within MMJJ_RS07945 at 1458.395 kb on + strand at 1458.433 kb on - strand, within MMJJ_RS07945 at 1458.603 kb on + strand at 1458.789 kb on + strand, within MMJJ_RS07950 at 1458.790 kb on - strand, within MMJJ_RS07950 at 1458.791 kb on + strand at 1458.848 kb on + strand, within MMJJ_RS07950 at 1458.848 kb on + strand, within MMJJ_RS07950 at 1458.848 kb on + strand, within MMJJ_RS07950 at 1458.881 kb on - strand, within MMJJ_RS07950 at 1458.881 kb on - strand, within MMJJ_RS07950 at 1459.043 kb on - strand at 1459.043 kb on - strand at 1459.045 kb on - strand at 1459.046 kb on + strand at 1459.046 kb on + strand at 1459.046 kb on + strand at 1459.046 kb on + strand at 1459.080 kb on + strand at 1459.080 kb on + strand at 1459.081 kb on - strand at 1459.088 kb on - strand at 1459.089 kb on + strand at 1459.101 kb on - strand at 1459.101 kb on - strand at 1459.103 kb on - strand at 1459.104 kb on + strand at 1459.104 kb on + strand at 1459.105 kb on - strand at 1459.105 kb on - strand at 1459.105 kb on - strand at 1459.105 kb on - strand at 1459.105 kb on - strand at 1459.105 kb on - strand at 1459.105 kb on - strand at 1459.105 kb on - strand at 1459.106 kb on + strand at 1459.106 kb on + strand at 1459.106 kb on + strand at 1459.106 kb on + strand at 1459.107 kb on - strand at 1459.107 kb on - strand at 1459.107 kb on - strand at 1459.108 kb on + strand at 1459.108 kb on + strand at 1459.108 kb on + strand at 1459.108 kb on + strand at 1459.136 kb on - strand at 1459.139 kb on + strand at 1459.139 kb on + strand at 1459.139 kb on + strand at 1459.139 kb on + strand at 1459.140 kb on - strand at 1459.141 kb on + strand at 1459.141 kb on + strand at 1459.187 kb on - strand at 1459.240 kb on - strand at 1459.339 kb on + strand, within MMJJ_RS07955 at 1459.427 kb on - strand, within MMJJ_RS07955 at 1459.428 kb on + strand, within MMJJ_RS07955 at 1459.428 kb on + strand, within MMJJ_RS07955 at 1459.428 kb on + strand, within MMJJ_RS07955 at 1459.428 kb on + strand, within MMJJ_RS07955 at 1459.428 kb on + strand, within MMJJ_RS07955 at 1459.464 kb on - strand, within MMJJ_RS07955 at 1459.464 kb on - strand, within MMJJ_RS07955 at 1459.465 kb on + strand, within MMJJ_RS07955 at 1459.465 kb on + strand, within MMJJ_RS07955 at 1459.537 kb on + strand, within MMJJ_RS07955 at 1459.537 kb on + strand, within MMJJ_RS07955 at 1459.565 kb on - strand, within MMJJ_RS07955 at 1459.565 kb on - strand, within MMJJ_RS07955 at 1459.587 kb on - strand, within MMJJ_RS07955 at 1459.619 kb on + strand, within MMJJ_RS07955 at 1459.623 kb on - strand, within MMJJ_RS07955 at 1459.624 kb on + strand, within MMJJ_RS07955 at 1459.624 kb on + strand, within MMJJ_RS07955 at 1459.626 kb on - strand, within MMJJ_RS07955 at 1459.627 kb on + strand, within MMJJ_RS07955 at 1459.627 kb on + strand, within MMJJ_RS07955 at 1459.627 kb on + strand, within MMJJ_RS07955 at 1459.654 kb on + strand, within MMJJ_RS07955 at 1459.661 kb on - strand, within MMJJ_RS07955 at 1459.774 kb on + strand, within MMJJ_RS07955 at 1459.783 kb on + strand, within MMJJ_RS07955 at 1459.802 kb on + strand, within MMJJ_RS07955 at 1459.802 kb on + strand, within MMJJ_RS07955 at 1459.802 kb on + strand, within MMJJ_RS07955 at 1459.808 kb on - strand, within MMJJ_RS07955 at 1459.809 kb on + strand, within MMJJ_RS07955 at 1459.809 kb on + strand, within MMJJ_RS07955 at 1459.936 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,457,655 - MMJJ_RS07945 0.12 -2.2 1,457,655 - -1.2 1,457,660 + MMJJ_RS07945 0.12 -2.2 1,457,682 - -0.8 1,457,686 - MMJJ_RS07945 0.15 -2.5 1,458,002 - MMJJ_RS07945 0.45 -1.8 1,458,016 - MMJJ_RS07945 0.46 -0.8 1,458,053 + -1.2 1,458,122 + MMJJ_RS07945 0.56 -1.9 1,458,395 + -0.8 1,458,433 - MMJJ_RS07945 0.85 -3.2 1,458,603 + -2.0 1,458,789 + MMJJ_RS07950 0.40 -1.8 1,458,790 - MMJJ_RS07950 0.40 -2.8 1,458,791 + -0.8 1,458,848 + MMJJ_RS07950 0.53 -3.0 1,458,848 + MMJJ_RS07950 0.53 -2.3 1,458,848 + MMJJ_RS07950 0.53 -3.2 1,458,881 - MMJJ_RS07950 0.60 -1.4 1,458,881 - MMJJ_RS07950 0.60 -2.0 1,459,043 - -1.3 1,459,043 - -2.5 1,459,045 - -1.8 1,459,046 + -2.0 1,459,046 + -2.5 1,459,046 + -1.8 1,459,046 + -2.4 1,459,080 + -2.4 1,459,080 + -2.8 1,459,081 - -2.9 1,459,088 - -2.0 1,459,089 + -3.2 1,459,101 - -2.3 1,459,101 - -1.7 1,459,103 - -1.2 1,459,104 + -1.2 1,459,104 + -1.8 1,459,105 - -2.6 1,459,105 - -2.2 1,459,105 - -3.2 1,459,105 - -4.0 1,459,105 - -3.4 1,459,105 - -4.5 1,459,105 - -3.5 1,459,105 - -2.2 1,459,106 + -1.7 1,459,106 + -2.7 1,459,106 + -2.6 1,459,106 + -3.2 1,459,107 - -2.9 1,459,107 - -3.4 1,459,107 - -2.2 1,459,108 + -1.2 1,459,108 + -0.3 1,459,108 + +0.0 1,459,108 + -0.1 1,459,136 - -0.4 1,459,139 + -1.5 1,459,139 + -0.7 1,459,139 + -0.1 1,459,139 + -1.2 1,459,140 - +0.2 1,459,141 + +0.5 1,459,141 + -0.0 1,459,187 - -1.2 1,459,240 - -0.2 1,459,339 + MMJJ_RS07955 0.13 -1.2 1,459,427 - MMJJ_RS07955 0.27 +0.4 1,459,428 + MMJJ_RS07955 0.27 -1.8 1,459,428 + MMJJ_RS07955 0.27 -1.5 1,459,428 + MMJJ_RS07955 0.27 -1.8 1,459,428 + MMJJ_RS07955 0.27 -1.5 1,459,428 + MMJJ_RS07955 0.27 -3.5 1,459,464 - MMJJ_RS07955 0.33 +1.2 1,459,464 - MMJJ_RS07955 0.33 -1.1 1,459,465 + MMJJ_RS07955 0.33 -1.9 1,459,465 + MMJJ_RS07955 0.33 +0.4 1,459,537 + MMJJ_RS07955 0.45 -1.5 1,459,537 + MMJJ_RS07955 0.45 -2.8 1,459,565 - MMJJ_RS07955 0.49 -2.5 1,459,565 - MMJJ_RS07955 0.49 -1.8 1,459,587 - MMJJ_RS07955 0.53 -2.1 1,459,619 + MMJJ_RS07955 0.58 -0.4 1,459,623 - MMJJ_RS07955 0.59 -0.3 1,459,624 + MMJJ_RS07955 0.59 -2.7 1,459,624 + MMJJ_RS07955 0.59 -2.2 1,459,626 - MMJJ_RS07955 0.59 -2.6 1,459,627 + MMJJ_RS07955 0.60 -0.9 1,459,627 + MMJJ_RS07955 0.60 -1.9 1,459,627 + MMJJ_RS07955 0.60 -4.1 1,459,654 + MMJJ_RS07955 0.64 -1.5 1,459,661 - MMJJ_RS07955 0.65 -1.8 1,459,774 + MMJJ_RS07955 0.83 -3.7 1,459,783 + MMJJ_RS07955 0.85 -1.4 1,459,802 + MMJJ_RS07955 0.88 -2.7 1,459,802 + MMJJ_RS07955 0.88 -3.6 1,459,802 + MMJJ_RS07955 0.88 -2.4 1,459,808 - MMJJ_RS07955 0.89 -2.0 1,459,809 + MMJJ_RS07955 0.89 -3.1 1,459,809 + MMJJ_RS07955 0.89 -2.9 1,459,936 + -1.2
Or see this region's nucleotide sequence