Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07800 and MMJJ_RS07805 are separated by 83 nucleotides MMJJ_RS07805 and MMJJ_RS07810 are separated by 10 nucleotides
MMJJ_RS07800: MMJJ_RS07800 - AMP-binding protein, at 1,428,217 to 1,429,890
_RS07800
MMJJ_RS07805: MMJJ_RS07805 - ferredoxin family protein, at 1,429,974 to 1,430,213
_RS07805
MMJJ_RS07810: MMJJ_RS07810 - 3-methyl-2-oxobutanoate dehydrogenase subunit VorB, at 1,430,224 to 1,431,279
_RS07810
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-2
-1
0
1 at 1428.984 kb on - strand, within MMJJ_RS07800 at 1428.984 kb on - strand, within MMJJ_RS07800 at 1428.985 kb on + strand, within MMJJ_RS07800 at 1429.095 kb on - strand, within MMJJ_RS07800 at 1429.096 kb on + strand, within MMJJ_RS07800 at 1429.116 kb on - strand, within MMJJ_RS07800 at 1429.116 kb on - strand, within MMJJ_RS07800 at 1429.146 kb on - strand, within MMJJ_RS07800 at 1429.147 kb on + strand, within MMJJ_RS07800 at 1429.147 kb on + strand, within MMJJ_RS07800 at 1429.172 kb on - strand, within MMJJ_RS07800 at 1429.172 kb on - strand, within MMJJ_RS07800 at 1429.172 kb on - strand, within MMJJ_RS07800 at 1429.173 kb on + strand, within MMJJ_RS07800 at 1429.185 kb on + strand, within MMJJ_RS07800 at 1429.203 kb on + strand, within MMJJ_RS07800 at 1429.245 kb on - strand, within MMJJ_RS07800 at 1429.246 kb on + strand, within MMJJ_RS07800 at 1429.246 kb on + strand, within MMJJ_RS07800 at 1429.251 kb on + strand, within MMJJ_RS07800 at 1429.283 kb on - strand, within MMJJ_RS07800 at 1429.283 kb on - strand, within MMJJ_RS07800 at 1429.283 kb on - strand, within MMJJ_RS07800 at 1429.284 kb on + strand, within MMJJ_RS07800 at 1429.284 kb on + strand, within MMJJ_RS07800 at 1429.391 kb on - strand, within MMJJ_RS07800 at 1429.391 kb on - strand, within MMJJ_RS07800 at 1429.392 kb on + strand, within MMJJ_RS07800 at 1429.492 kb on - strand, within MMJJ_RS07800 at 1429.493 kb on + strand, within MMJJ_RS07800 at 1429.505 kb on + strand, within MMJJ_RS07800 at 1429.544 kb on + strand, within MMJJ_RS07800 at 1429.626 kb on - strand, within MMJJ_RS07800 at 1429.626 kb on - strand, within MMJJ_RS07800 at 1429.626 kb on - strand, within MMJJ_RS07800 at 1429.626 kb on - strand, within MMJJ_RS07800 at 1429.627 kb on + strand, within MMJJ_RS07800 at 1429.644 kb on + strand, within MMJJ_RS07800 at 1429.644 kb on + strand, within MMJJ_RS07800 at 1429.658 kb on - strand, within MMJJ_RS07800 at 1429.658 kb on - strand, within MMJJ_RS07800 at 1429.659 kb on + strand, within MMJJ_RS07800 at 1429.659 kb on + strand, within MMJJ_RS07800 at 1429.659 kb on + strand, within MMJJ_RS07800 at 1429.695 kb on - strand, within MMJJ_RS07800 at 1429.701 kb on - strand, within MMJJ_RS07800 at 1429.702 kb on + strand, within MMJJ_RS07800 at 1429.787 kb on + strand at 1429.792 kb on - strand at 1429.892 kb on - strand at 1429.896 kb on - strand at 1429.896 kb on - strand at 1429.897 kb on + strand at 1429.919 kb on + strand at 1429.919 kb on + strand at 1429.923 kb on - strand at 1429.923 kb on - strand at 1429.923 kb on - strand at 1429.924 kb on + strand at 1429.924 kb on + strand at 1429.957 kb on - strand at 1429.958 kb on + strand at 1429.958 kb on + strand at 1429.958 kb on + strand at 1429.974 kb on + strand at 1430.006 kb on + strand, within MMJJ_RS07805 at 1430.010 kb on + strand, within MMJJ_RS07805 at 1430.010 kb on + strand, within MMJJ_RS07805 at 1430.044 kb on - strand, within MMJJ_RS07805 at 1430.044 kb on - strand, within MMJJ_RS07805 at 1430.044 kb on - strand, within MMJJ_RS07805 at 1430.100 kb on - strand, within MMJJ_RS07805 at 1430.101 kb on + strand, within MMJJ_RS07805 at 1430.103 kb on + strand, within MMJJ_RS07805 at 1430.103 kb on + strand, within MMJJ_RS07805 at 1430.106 kb on - strand, within MMJJ_RS07805 at 1430.110 kb on - strand, within MMJJ_RS07805 at 1430.115 kb on - strand, within MMJJ_RS07805 at 1430.154 kb on - strand, within MMJJ_RS07805 at 1430.155 kb on + strand, within MMJJ_RS07805 at 1430.155 kb on + strand, within MMJJ_RS07805 at 1430.180 kb on + strand, within MMJJ_RS07805 at 1430.186 kb on + strand, within MMJJ_RS07805 at 1430.211 kb on - strand at 1430.220 kb on - strand at 1430.221 kb on + strand at 1430.221 kb on + strand at 1430.221 kb on + strand at 1430.221 kb on + strand at 1430.221 kb on + strand at 1430.221 kb on + strand at 1430.275 kb on - strand at 1430.275 kb on - strand at 1430.275 kb on - strand at 1430.275 kb on - strand at 1430.275 kb on - strand at 1430.275 kb on - strand at 1430.275 kb on - strand at 1430.276 kb on + strand at 1430.276 kb on + strand at 1430.293 kb on - strand at 1430.293 kb on - strand at 1430.294 kb on + strand at 1430.327 kb on - strand at 1430.328 kb on + strand at 1430.328 kb on + strand at 1430.328 kb on + strand at 1430.347 kb on - strand, within MMJJ_RS07810 at 1430.402 kb on - strand, within MMJJ_RS07810 at 1430.406 kb on - strand, within MMJJ_RS07810 at 1430.407 kb on + strand, within MMJJ_RS07810 at 1430.444 kb on - strand, within MMJJ_RS07810 at 1430.445 kb on + strand, within MMJJ_RS07810 at 1430.528 kb on - strand, within MMJJ_RS07810 at 1430.529 kb on + strand, within MMJJ_RS07810 at 1430.529 kb on + strand, within MMJJ_RS07810 at 1430.529 kb on + strand, within MMJJ_RS07810 at 1430.584 kb on - strand, within MMJJ_RS07810 at 1430.598 kb on + strand, within MMJJ_RS07810 at 1430.701 kb on - strand, within MMJJ_RS07810 at 1430.701 kb on - strand, within MMJJ_RS07810 at 1430.780 kb on - strand, within MMJJ_RS07810 at 1430.781 kb on + strand, within MMJJ_RS07810 at 1430.858 kb on - strand, within MMJJ_RS07810 at 1430.888 kb on + strand, within MMJJ_RS07810 at 1430.944 kb on - strand, within MMJJ_RS07810 at 1430.945 kb on + strand, within MMJJ_RS07810 at 1430.945 kb on + strand, within MMJJ_RS07810 at 1430.945 kb on + strand, within MMJJ_RS07810 at 1430.951 kb on - strand, within MMJJ_RS07810 at 1430.952 kb on + strand, within MMJJ_RS07810 at 1430.980 kb on - strand, within MMJJ_RS07810 at 1431.039 kb on + strand, within MMJJ_RS07810 at 1431.068 kb on - strand, within MMJJ_RS07810 at 1431.069 kb on + strand, within MMJJ_RS07810 at 1431.123 kb on - strand, within MMJJ_RS07810 at 1431.124 kb on + strand, within MMJJ_RS07810
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,428,984 - MMJJ_RS07800 0.46 +0.4 1,428,984 - MMJJ_RS07800 0.46 +0.3 1,428,985 + MMJJ_RS07800 0.46 +0.9 1,429,095 - MMJJ_RS07800 0.52 -0.9 1,429,096 + MMJJ_RS07800 0.53 -1.1 1,429,116 - MMJJ_RS07800 0.54 -0.1 1,429,116 - MMJJ_RS07800 0.54 -0.1 1,429,146 - MMJJ_RS07800 0.55 +0.5 1,429,147 + MMJJ_RS07800 0.56 +0.4 1,429,147 + MMJJ_RS07800 0.56 +0.4 1,429,172 - MMJJ_RS07800 0.57 -0.5 1,429,172 - MMJJ_RS07800 0.57 +0.8 1,429,172 - MMJJ_RS07800 0.57 +0.2 1,429,173 + MMJJ_RS07800 0.57 -0.5 1,429,185 + MMJJ_RS07800 0.58 +0.3 1,429,203 + MMJJ_RS07800 0.59 -0.7 1,429,245 - MMJJ_RS07800 0.61 -0.2 1,429,246 + MMJJ_RS07800 0.61 +0.3 1,429,246 + MMJJ_RS07800 0.61 +1.1 1,429,251 + MMJJ_RS07800 0.62 -0.7 1,429,283 - MMJJ_RS07800 0.64 +0.6 1,429,283 - MMJJ_RS07800 0.64 +0.0 1,429,283 - MMJJ_RS07800 0.64 +0.3 1,429,284 + MMJJ_RS07800 0.64 +0.8 1,429,284 + MMJJ_RS07800 0.64 -0.1 1,429,391 - MMJJ_RS07800 0.70 +0.7 1,429,391 - MMJJ_RS07800 0.70 +1.2 1,429,392 + MMJJ_RS07800 0.70 -0.0 1,429,492 - MMJJ_RS07800 0.76 +1.3 1,429,493 + MMJJ_RS07800 0.76 -0.2 1,429,505 + MMJJ_RS07800 0.77 +0.5 1,429,544 + MMJJ_RS07800 0.79 +0.2 1,429,626 - MMJJ_RS07800 0.84 -0.4 1,429,626 - MMJJ_RS07800 0.84 -0.9 1,429,626 - MMJJ_RS07800 0.84 -1.3 1,429,626 - MMJJ_RS07800 0.84 -1.3 1,429,627 + MMJJ_RS07800 0.84 +0.4 1,429,644 + MMJJ_RS07800 0.85 +1.0 1,429,644 + MMJJ_RS07800 0.85 +0.7 1,429,658 - MMJJ_RS07800 0.86 +0.3 1,429,658 - MMJJ_RS07800 0.86 +0.8 1,429,659 + MMJJ_RS07800 0.86 -2.7 1,429,659 + MMJJ_RS07800 0.86 -0.2 1,429,659 + MMJJ_RS07800 0.86 -1.2 1,429,695 - MMJJ_RS07800 0.88 +0.9 1,429,701 - MMJJ_RS07800 0.89 +1.1 1,429,702 + MMJJ_RS07800 0.89 -0.5 1,429,787 + +0.8 1,429,792 - +0.3 1,429,892 - -0.4 1,429,896 - -0.2 1,429,896 - +0.1 1,429,897 + +0.3 1,429,919 + +1.4 1,429,919 + +0.3 1,429,923 - -0.3 1,429,923 - +0.5 1,429,923 - +0.1 1,429,924 + -0.2 1,429,924 + +0.6 1,429,957 - +0.7 1,429,958 + +0.8 1,429,958 + +0.2 1,429,958 + +0.0 1,429,974 + -0.7 1,430,006 + MMJJ_RS07805 0.13 +0.5 1,430,010 + MMJJ_RS07805 0.15 +1.1 1,430,010 + MMJJ_RS07805 0.15 +0.6 1,430,044 - MMJJ_RS07805 0.29 -2.4 1,430,044 - MMJJ_RS07805 0.29 +0.2 1,430,044 - MMJJ_RS07805 0.29 -1.4 1,430,100 - MMJJ_RS07805 0.53 -1.1 1,430,101 + MMJJ_RS07805 0.53 +0.2 1,430,103 + MMJJ_RS07805 0.54 -2.2 1,430,103 + MMJJ_RS07805 0.54 +0.7 1,430,106 - MMJJ_RS07805 0.55 +0.2 1,430,110 - MMJJ_RS07805 0.57 +1.6 1,430,115 - MMJJ_RS07805 0.59 +0.6 1,430,154 - MMJJ_RS07805 0.75 +0.3 1,430,155 + MMJJ_RS07805 0.75 -1.6 1,430,155 + MMJJ_RS07805 0.75 +1.0 1,430,180 + MMJJ_RS07805 0.86 -0.9 1,430,186 + MMJJ_RS07805 0.88 -0.7 1,430,211 - +0.3 1,430,220 - +0.6 1,430,221 + +0.4 1,430,221 + +0.7 1,430,221 + -0.4 1,430,221 + -0.6 1,430,221 + +1.3 1,430,221 + +0.6 1,430,275 - +0.6 1,430,275 - -0.4 1,430,275 - +0.8 1,430,275 - +0.1 1,430,275 - +1.5 1,430,275 - +0.3 1,430,275 - +0.3 1,430,276 + +0.3 1,430,276 + +0.4 1,430,293 - -0.1 1,430,293 - -0.6 1,430,294 + +0.3 1,430,327 - +0.8 1,430,328 + -0.2 1,430,328 + -0.8 1,430,328 + -0.1 1,430,347 - MMJJ_RS07810 0.12 -0.2 1,430,402 - MMJJ_RS07810 0.17 +0.9 1,430,406 - MMJJ_RS07810 0.17 -0.4 1,430,407 + MMJJ_RS07810 0.17 +0.3 1,430,444 - MMJJ_RS07810 0.21 +0.7 1,430,445 + MMJJ_RS07810 0.21 -0.1 1,430,528 - MMJJ_RS07810 0.29 +0.4 1,430,529 + MMJJ_RS07810 0.29 -0.9 1,430,529 + MMJJ_RS07810 0.29 +0.4 1,430,529 + MMJJ_RS07810 0.29 +0.6 1,430,584 - MMJJ_RS07810 0.34 -0.3 1,430,598 + MMJJ_RS07810 0.35 -0.3 1,430,701 - MMJJ_RS07810 0.45 +1.3 1,430,701 - MMJJ_RS07810 0.45 -0.5 1,430,780 - MMJJ_RS07810 0.53 -0.2 1,430,781 + MMJJ_RS07810 0.53 -0.3 1,430,858 - MMJJ_RS07810 0.60 +0.4 1,430,888 + MMJJ_RS07810 0.63 +0.2 1,430,944 - MMJJ_RS07810 0.68 +0.6 1,430,945 + MMJJ_RS07810 0.68 -1.3 1,430,945 + MMJJ_RS07810 0.68 +1.0 1,430,945 + MMJJ_RS07810 0.68 +0.8 1,430,951 - MMJJ_RS07810 0.69 +0.2 1,430,952 + MMJJ_RS07810 0.69 +1.0 1,430,980 - MMJJ_RS07810 0.72 -0.6 1,431,039 + MMJJ_RS07810 0.77 -0.3 1,431,068 - MMJJ_RS07810 0.80 -0.9 1,431,069 + MMJJ_RS07810 0.80 -0.1 1,431,123 - MMJJ_RS07810 0.85 -1.1 1,431,124 + MMJJ_RS07810 0.85 +0.6
Or see this region's nucleotide sequence