Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07705 and MMJJ_RS07710 are separated by 125 nucleotides MMJJ_RS07710 and MMJJ_RS07715 are separated by 62 nucleotides MMJJ_RS07715 and MMJJ_RS07720 are separated by 39 nucleotides MMJJ_RS07720 and MMJJ_RS07725 are separated by 33 nucleotides
MMJJ_RS07705: MMJJ_RS07705 - GTP-binding protein EngB, at 1,414,319 to 1,415,011
_RS07705
MMJJ_RS07710: MMJJ_RS07710 - 50S ribosomal protein L16, at 1,415,137 to 1,415,658
_RS07710
MMJJ_RS07715: MMJJ_RS07715 - UbiD family decarboxylase, at 1,415,721 to 1,416,998
_RS07715
MMJJ_RS07720: MMJJ_RS07720 - UPF0058 family protein, at 1,417,038 to 1,417,331
_RS07720
MMJJ_RS07725: MMJJ_RS07725 - DNA primase DnaG, at 1,417,365 to 1,418,675
_RS07725
Position (kb)
1415
1416
1417 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1414.813 kb on + strand, within MMJJ_RS07705 at 1414.987 kb on - strand at 1414.987 kb on - strand at 1414.988 kb on + strand at 1414.988 kb on + strand at 1415.686 kb on - strand at 1415.706 kb on - strand at 1415.717 kb on + strand at 1415.717 kb on + strand at 1415.723 kb on + strand at 1415.952 kb on - strand, within MMJJ_RS07715 at 1415.953 kb on + strand, within MMJJ_RS07715 at 1416.216 kb on + strand, within MMJJ_RS07715 at 1416.607 kb on + strand, within MMJJ_RS07715 at 1416.637 kb on + strand, within MMJJ_RS07715 at 1416.677 kb on + strand, within MMJJ_RS07715 at 1416.802 kb on + strand, within MMJJ_RS07715 at 1417.159 kb on + strand, within MMJJ_RS07720 at 1417.175 kb on - strand, within MMJJ_RS07720 at 1417.175 kb on - strand, within MMJJ_RS07720 at 1417.287 kb on + strand, within MMJJ_RS07720 at 1417.306 kb on + strand at 1417.485 kb on - strand at 1417.620 kb on - strand, within MMJJ_RS07725 at 1417.667 kb on - strand, within MMJJ_RS07725 at 1417.680 kb on - strand, within MMJJ_RS07725 at 1417.680 kb on - strand, within MMJJ_RS07725 at 1417.682 kb on - strand, within MMJJ_RS07725 at 1417.683 kb on + strand, within MMJJ_RS07725 at 1417.704 kb on - strand at 1417.714 kb on + strand at 1417.716 kb on - strand, within MMJJ_RS07725 at 1417.716 kb on - strand, within MMJJ_RS07725 at 1417.718 kb on - strand, within MMJJ_RS07725 at 1417.719 kb on + strand, within MMJJ_RS07725 at 1417.833 kb on - strand, within MMJJ_RS07725 at 1417.853 kb on - strand, within MMJJ_RS07725 at 1417.908 kb on - strand, within MMJJ_RS07725
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,414,813 + MMJJ_RS07705 0.71 -2.3 1,414,987 - -0.3 1,414,987 - -0.2 1,414,988 + +1.1 1,414,988 + +0.2 1,415,686 - -1.1 1,415,706 - -0.6 1,415,717 + -0.8 1,415,717 + +0.3 1,415,723 + +0.8 1,415,952 - MMJJ_RS07715 0.18 -0.7 1,415,953 + MMJJ_RS07715 0.18 -1.5 1,416,216 + MMJJ_RS07715 0.39 -2.7 1,416,607 + MMJJ_RS07715 0.69 -1.9 1,416,637 + MMJJ_RS07715 0.72 -4.1 1,416,677 + MMJJ_RS07715 0.75 -1.2 1,416,802 + MMJJ_RS07715 0.85 +0.3 1,417,159 + MMJJ_RS07720 0.41 -0.7 1,417,175 - MMJJ_RS07720 0.47 -3.1 1,417,175 - MMJJ_RS07720 0.47 -3.9 1,417,287 + MMJJ_RS07720 0.85 -1.3 1,417,306 + -3.4 1,417,485 - -3.9 1,417,620 - MMJJ_RS07725 0.19 -0.3 1,417,667 - MMJJ_RS07725 0.23 -3.1 1,417,680 - MMJJ_RS07725 0.24 -2.5 1,417,680 - MMJJ_RS07725 0.24 -1.5 1,417,682 - MMJJ_RS07725 0.24 -1.9 1,417,683 + MMJJ_RS07725 0.24 +0.9 1,417,704 - -1.1 1,417,714 + -1.1 1,417,716 - MMJJ_RS07725 0.27 -3.3 1,417,716 - MMJJ_RS07725 0.27 -1.5 1,417,718 - MMJJ_RS07725 0.27 -2.9 1,417,719 + MMJJ_RS07725 0.27 -2.7 1,417,833 - MMJJ_RS07725 0.36 -3.4 1,417,853 - MMJJ_RS07725 0.37 -3.7 1,417,908 - MMJJ_RS07725 0.41 -2.7
Or see this region's nucleotide sequence