Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07370 and MMJJ_RS07375 are separated by 138 nucleotides MMJJ_RS07375 and MMJJ_RS07380 are separated by 90 nucleotides
MMJJ_RS07370: MMJJ_RS07370 - tRNA 4-thiouridine(8) synthase ThiI, at 1,353,201 to 1,354,352
_RS07370
MMJJ_RS07375: MMJJ_RS07375 - tRNA 2-thiocytidine biosynthesis TtcA family protein, at 1,354,491 to 1,355,465
_RS07375
MMJJ_RS07380: MMJJ_RS07380 - sulfide-dependent adenosine diphosphate thiazole synthase, at 1,355,556 to 1,356,344
_RS07380
Position (kb)
1354
1355
1356 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1353.541 kb on - strand, within MMJJ_RS07370 at 1353.565 kb on - strand, within MMJJ_RS07370 at 1353.627 kb on - strand, within MMJJ_RS07370 at 1353.634 kb on + strand at 1353.663 kb on - strand, within MMJJ_RS07370 at 1353.726 kb on - strand at 1353.758 kb on + strand, within MMJJ_RS07370 at 1353.758 kb on + strand, within MMJJ_RS07370 at 1353.913 kb on + strand, within MMJJ_RS07370 at 1353.918 kb on - strand at 1354.110 kb on - strand, within MMJJ_RS07370 at 1354.111 kb on + strand, within MMJJ_RS07370 at 1354.111 kb on + strand, within MMJJ_RS07370 at 1354.115 kb on + strand, within MMJJ_RS07370 at 1354.117 kb on - strand at 1354.128 kb on + strand, within MMJJ_RS07370 at 1354.134 kb on - strand, within MMJJ_RS07370 at 1354.198 kb on + strand, within MMJJ_RS07370 at 1354.354 kb on + strand at 1354.386 kb on - strand at 1354.387 kb on + strand at 1354.396 kb on - strand at 1354.401 kb on + strand at 1354.406 kb on + strand at 1354.409 kb on - strand at 1354.409 kb on - strand at 1354.410 kb on + strand at 1354.410 kb on + strand at 1354.416 kb on - strand at 1354.420 kb on - strand at 1354.420 kb on - strand at 1354.421 kb on + strand at 1354.425 kb on + strand at 1354.425 kb on + strand at 1354.425 kb on + strand at 1354.438 kb on - strand at 1354.438 kb on - strand at 1354.438 kb on - strand at 1354.438 kb on - strand at 1354.439 kb on + strand at 1354.439 kb on + strand at 1354.439 kb on + strand at 1354.439 kb on + strand at 1354.445 kb on - strand at 1354.445 kb on - strand at 1354.445 kb on - strand at 1354.445 kb on - strand at 1354.445 kb on - strand at 1354.446 kb on + strand at 1354.447 kb on - strand at 1354.447 kb on - strand at 1354.448 kb on + strand at 1354.448 kb on + strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.450 kb on + strand at 1354.450 kb on + strand at 1354.450 kb on + strand at 1354.450 kb on + strand at 1354.450 kb on + strand at 1354.457 kb on - strand at 1354.457 kb on - strand at 1354.457 kb on - strand at 1354.458 kb on + strand at 1354.458 kb on + strand at 1354.458 kb on + strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.463 kb on - strand at 1354.463 kb on - strand at 1354.463 kb on - strand at 1354.463 kb on - strand at 1354.463 kb on - strand at 1354.463 kb on - strand at 1354.464 kb on + strand at 1354.464 kb on + strand at 1354.464 kb on + strand at 1354.474 kb on - strand at 1354.501 kb on - strand at 1354.502 kb on + strand at 1354.534 kb on + strand at 1354.538 kb on + strand at 1354.544 kb on + strand at 1354.560 kb on + strand at 1354.723 kb on - strand, within MMJJ_RS07375 at 1354.828 kb on - strand, within MMJJ_RS07375 at 1354.828 kb on - strand, within MMJJ_RS07375 at 1354.834 kb on - strand, within MMJJ_RS07375 at 1354.835 kb on + strand, within MMJJ_RS07375 at 1354.835 kb on + strand, within MMJJ_RS07375 at 1354.903 kb on - strand, within MMJJ_RS07375 at 1354.907 kb on - strand, within MMJJ_RS07375 at 1355.129 kb on - strand at 1355.150 kb on + strand, within MMJJ_RS07375 at 1355.191 kb on - strand, within MMJJ_RS07375 at 1355.191 kb on - strand, within MMJJ_RS07375 at 1355.212 kb on - strand, within MMJJ_RS07375 at 1355.212 kb on - strand, within MMJJ_RS07375 at 1355.213 kb on + strand, within MMJJ_RS07375 at 1355.231 kb on - strand, within MMJJ_RS07375 at 1355.232 kb on + strand, within MMJJ_RS07375 at 1355.250 kb on + strand, within MMJJ_RS07375 at 1355.281 kb on - strand, within MMJJ_RS07375 at 1355.301 kb on - strand, within MMJJ_RS07375 at 1355.302 kb on + strand, within MMJJ_RS07375 at 1355.302 kb on + strand, within MMJJ_RS07375 at 1355.302 kb on + strand, within MMJJ_RS07375 at 1355.304 kb on - strand, within MMJJ_RS07375 at 1355.305 kb on + strand, within MMJJ_RS07375 at 1355.339 kb on - strand, within MMJJ_RS07375 at 1355.421 kb on + strand at 1355.435 kb on + strand at 1355.435 kb on + strand at 1355.438 kb on - strand at 1355.438 kb on - strand at 1355.439 kb on + strand at 1355.441 kb on - strand at 1355.441 kb on - strand at 1355.441 kb on - strand at 1355.442 kb on + strand at 1355.451 kb on - strand at 1355.452 kb on + strand at 1355.452 kb on + strand at 1355.452 kb on - strand at 1355.453 kb on - strand at 1355.453 kb on - strand at 1355.453 kb on - strand at 1355.466 kb on - strand at 1355.466 kb on - strand at 1355.474 kb on - strand at 1355.477 kb on + strand at 1355.526 kb on - strand at 1355.527 kb on + strand at 1355.534 kb on - strand at 1355.585 kb on + strand at 1355.653 kb on - strand, within MMJJ_RS07380 at 1355.653 kb on - strand at 1355.681 kb on - strand, within MMJJ_RS07380 at 1355.681 kb on - strand, within MMJJ_RS07380 at 1355.816 kb on + strand, within MMJJ_RS07380 at 1355.839 kb on - strand, within MMJJ_RS07380 at 1355.839 kb on - strand, within MMJJ_RS07380 at 1355.903 kb on + strand, within MMJJ_RS07380 at 1355.936 kb on + strand, within MMJJ_RS07380 at 1355.963 kb on + strand, within MMJJ_RS07380 at 1356.035 kb on + strand, within MMJJ_RS07380 at 1356.049 kb on - strand, within MMJJ_RS07380 at 1356.049 kb on - strand, within MMJJ_RS07380 at 1356.061 kb on + strand, within MMJJ_RS07380 at 1356.064 kb on - strand, within MMJJ_RS07380 at 1356.065 kb on + strand, within MMJJ_RS07380 at 1356.067 kb on - strand, within MMJJ_RS07380 at 1356.102 kb on + strand, within MMJJ_RS07380 at 1356.120 kb on + strand, within MMJJ_RS07380 at 1356.188 kb on - strand, within MMJJ_RS07380 at 1356.188 kb on - strand, within MMJJ_RS07380 at 1356.205 kb on - strand, within MMJJ_RS07380 at 1356.206 kb on + strand, within MMJJ_RS07380 at 1356.219 kb on - strand, within MMJJ_RS07380 at 1356.219 kb on - strand, within MMJJ_RS07380 at 1356.220 kb on + strand, within MMJJ_RS07380 at 1356.220 kb on + strand, within MMJJ_RS07380 at 1356.246 kb on - strand, within MMJJ_RS07380 at 1356.288 kb on - strand at 1356.338 kb on - strand at 1356.338 kb on - strand at 1356.339 kb on + strand at 1356.342 kb on - strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.367 kb on - strand at 1356.376 kb on + strand at 1356.404 kb on - strand at 1356.455 kb on + strand at 1356.455 kb on + strand at 1356.458 kb on - strand at 1356.458 kb on - strand at 1356.458 kb on - strand at 1356.459 kb on + strand at 1356.460 kb on - strand at 1356.460 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.464 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,353,541 - MMJJ_RS07370 0.30 -0.3 1,353,565 - MMJJ_RS07370 0.32 -1.0 1,353,627 - MMJJ_RS07370 0.37 +0.1 1,353,634 + -0.3 1,353,663 - MMJJ_RS07370 0.40 -2.6 1,353,726 - -0.7 1,353,758 + MMJJ_RS07370 0.48 -1.3 1,353,758 + MMJJ_RS07370 0.48 -2.1 1,353,913 + MMJJ_RS07370 0.62 -2.5 1,353,918 - -0.3 1,354,110 - MMJJ_RS07370 0.79 +1.3 1,354,111 + MMJJ_RS07370 0.79 -0.7 1,354,111 + MMJJ_RS07370 0.79 -1.9 1,354,115 + MMJJ_RS07370 0.79 -3.1 1,354,117 - -0.3 1,354,128 + MMJJ_RS07370 0.80 -2.5 1,354,134 - MMJJ_RS07370 0.81 -3.9 1,354,198 + MMJJ_RS07370 0.87 -1.0 1,354,354 + -3.6 1,354,386 - +0.5 1,354,387 + -1.3 1,354,396 - -0.0 1,354,401 + -0.7 1,354,406 + -1.7 1,354,409 - +0.7 1,354,409 - -0.1 1,354,410 + -3.3 1,354,410 + -1.5 1,354,416 - +1.2 1,354,420 - +0.6 1,354,420 - -2.5 1,354,421 + -1.8 1,354,425 + +1.2 1,354,425 + +0.7 1,354,425 + -0.1 1,354,438 - +0.1 1,354,438 - -0.2 1,354,438 - -0.1 1,354,438 - +0.0 1,354,439 + +0.5 1,354,439 + -1.3 1,354,439 + -0.0 1,354,439 + +0.1 1,354,445 - +0.2 1,354,445 - +1.1 1,354,445 - +0.4 1,354,445 - +0.1 1,354,445 - +1.9 1,354,446 + +0.5 1,354,447 - +1.5 1,354,447 - -0.4 1,354,448 + +1.0 1,354,448 + +1.4 1,354,449 - +0.5 1,354,449 - +1.1 1,354,449 - +1.1 1,354,449 - +0.5 1,354,449 - +0.2 1,354,449 - +1.2 1,354,449 - +1.1 1,354,449 - -0.7 1,354,450 + +0.6 1,354,450 + +0.4 1,354,450 + +0.3 1,354,450 + +0.1 1,354,450 + +0.0 1,354,457 - +0.3 1,354,457 - +0.7 1,354,457 - -0.2 1,354,458 + +0.4 1,354,458 + -0.5 1,354,458 + +0.5 1,354,459 - +0.5 1,354,459 - -0.3 1,354,459 - -0.1 1,354,459 - +0.5 1,354,459 - +1.9 1,354,459 - +0.8 1,354,459 - +0.4 1,354,460 + -0.3 1,354,460 + -0.2 1,354,460 + -0.5 1,354,460 + +1.0 1,354,460 + +0.4 1,354,460 + +0.0 1,354,460 + +0.9 1,354,463 - -0.2 1,354,463 - +0.4 1,354,463 - +0.1 1,354,463 - -0.4 1,354,463 - +0.0 1,354,463 - +1.7 1,354,464 + -0.0 1,354,464 + +0.5 1,354,464 + +1.3 1,354,474 - -0.8 1,354,501 - -0.1 1,354,502 + -1.5 1,354,534 + -1.0 1,354,538 + -1.7 1,354,544 + -2.8 1,354,560 + -0.7 1,354,723 - MMJJ_RS07375 0.24 -2.9 1,354,828 - MMJJ_RS07375 0.35 -2.1 1,354,828 - MMJJ_RS07375 0.35 -1.6 1,354,834 - MMJJ_RS07375 0.35 -4.2 1,354,835 + MMJJ_RS07375 0.35 -1.3 1,354,835 + MMJJ_RS07375 0.35 -0.7 1,354,903 - MMJJ_RS07375 0.42 -3.1 1,354,907 - MMJJ_RS07375 0.43 -3.2 1,355,129 - -1.7 1,355,150 + MMJJ_RS07375 0.68 -2.9 1,355,191 - MMJJ_RS07375 0.72 -1.8 1,355,191 - MMJJ_RS07375 0.72 +0.1 1,355,212 - MMJJ_RS07375 0.74 -1.3 1,355,212 - MMJJ_RS07375 0.74 -2.2 1,355,213 + MMJJ_RS07375 0.74 -1.9 1,355,231 - MMJJ_RS07375 0.76 -2.4 1,355,232 + MMJJ_RS07375 0.76 -1.6 1,355,250 + MMJJ_RS07375 0.78 -2.6 1,355,281 - MMJJ_RS07375 0.81 -2.5 1,355,301 - MMJJ_RS07375 0.83 -2.3 1,355,302 + MMJJ_RS07375 0.83 -1.3 1,355,302 + MMJJ_RS07375 0.83 -4.5 1,355,302 + MMJJ_RS07375 0.83 -1.8 1,355,304 - MMJJ_RS07375 0.83 -2.8 1,355,305 + MMJJ_RS07375 0.83 -2.6 1,355,339 - MMJJ_RS07375 0.87 -2.3 1,355,421 + -2.3 1,355,435 + -1.0 1,355,435 + -0.3 1,355,438 - -0.8 1,355,438 - -2.1 1,355,439 + -3.1 1,355,441 - -2.2 1,355,441 - -1.0 1,355,441 - -1.5 1,355,442 + -3.1 1,355,451 - -0.7 1,355,452 + +0.7 1,355,452 + +4.4 1,355,452 - +0.6 1,355,453 - -1.1 1,355,453 - -3.3 1,355,453 - -1.8 1,355,466 - -2.6 1,355,466 - -1.8 1,355,474 - -3.2 1,355,477 + -4.3 1,355,526 - -2.1 1,355,527 + -4.3 1,355,534 - -1.0 1,355,585 + -1.0 1,355,653 - MMJJ_RS07380 0.12 -2.1 1,355,653 - -1.0 1,355,681 - MMJJ_RS07380 0.16 -3.1 1,355,681 - MMJJ_RS07380 0.16 -4.1 1,355,816 + MMJJ_RS07380 0.33 -3.7 1,355,839 - MMJJ_RS07380 0.36 -3.7 1,355,839 - MMJJ_RS07380 0.36 -1.9 1,355,903 + MMJJ_RS07380 0.44 -3.6 1,355,936 + MMJJ_RS07380 0.48 -1.7 1,355,963 + MMJJ_RS07380 0.52 -2.0 1,356,035 + MMJJ_RS07380 0.61 -2.9 1,356,049 - MMJJ_RS07380 0.62 -3.2 1,356,049 - MMJJ_RS07380 0.62 -1.6 1,356,061 + MMJJ_RS07380 0.64 -2.7 1,356,064 - MMJJ_RS07380 0.64 -3.1 1,356,065 + MMJJ_RS07380 0.65 -1.8 1,356,067 - MMJJ_RS07380 0.65 -4.0 1,356,102 + MMJJ_RS07380 0.69 -4.1 1,356,120 + MMJJ_RS07380 0.71 -2.2 1,356,188 - MMJJ_RS07380 0.80 -3.8 1,356,188 - MMJJ_RS07380 0.80 -3.1 1,356,205 - MMJJ_RS07380 0.82 -3.4 1,356,206 + MMJJ_RS07380 0.82 -2.4 1,356,219 - MMJJ_RS07380 0.84 -2.2 1,356,219 - MMJJ_RS07380 0.84 -1.5 1,356,220 + MMJJ_RS07380 0.84 +0.9 1,356,220 + MMJJ_RS07380 0.84 -1.7 1,356,246 - MMJJ_RS07380 0.87 -2.9 1,356,288 - -1.7 1,356,338 - +1.1 1,356,338 - +0.1 1,356,339 + +0.7 1,356,342 - +0.8 1,356,354 + +0.3 1,356,354 + +1.0 1,356,354 + -2.9 1,356,354 + +0.4 1,356,367 - +0.4 1,356,376 + -0.2 1,356,404 - +1.0 1,356,455 + +0.4 1,356,455 + +0.8 1,356,458 - -0.2 1,356,458 - -0.2 1,356,458 - +0.0 1,356,459 + -0.4 1,356,460 - +1.2 1,356,460 - +1.1 1,356,463 - +0.8 1,356,463 - +1.6 1,356,463 - -1.0 1,356,463 - -1.5 1,356,464 + +1.0
Or see this region's nucleotide sequence