Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06740 and MMJJ_RS06745 overlap by 1 nucleotides MMJJ_RS06745 and MMJJ_RS06750 are separated by 172 nucleotides MMJJ_RS06750 and MMJJ_RS06755 are separated by 113 nucleotides
MMJJ_RS06740: MMJJ_RS06740 - energy-coupling factor ABC transporter substrate-binding protein, at 1,244,582 to 1,244,878
_RS06740
MMJJ_RS06745: MMJJ_RS06745 - energy-coupling factor ABC transporter permease, at 1,244,878 to 1,245,555
_RS06745
MMJJ_RS06750: MMJJ_RS06750 - homoaconitase large subunit, at 1,245,728 to 1,246,984
_RS06750
MMJJ_RS06755: MMJJ_RS06755 - diadenylate cyclase, at 1,247,098 to 1,248,036
_RS06755
Position (kb)
1245
1246
1247 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1244.785 kb on + strand, within MMJJ_RS06740 at 1244.812 kb on + strand, within MMJJ_RS06740 at 1244.829 kb on + strand, within MMJJ_RS06740 at 1244.834 kb on + strand, within MMJJ_RS06740 at 1244.840 kb on - strand, within MMJJ_RS06740 at 1244.841 kb on + strand, within MMJJ_RS06740 at 1244.841 kb on + strand, within MMJJ_RS06740 at 1244.841 kb on + strand, within MMJJ_RS06740 at 1244.913 kb on - strand at 1244.957 kb on - strand, within MMJJ_RS06745 at 1244.958 kb on + strand, within MMJJ_RS06745 at 1244.968 kb on + strand, within MMJJ_RS06745 at 1244.968 kb on + strand, within MMJJ_RS06745 at 1244.968 kb on + strand, within MMJJ_RS06745 at 1244.977 kb on - strand, within MMJJ_RS06745 at 1244.980 kb on - strand, within MMJJ_RS06745 at 1245.023 kb on + strand, within MMJJ_RS06745 at 1245.096 kb on - strand, within MMJJ_RS06745 at 1245.096 kb on - strand, within MMJJ_RS06745 at 1245.101 kb on - strand, within MMJJ_RS06745 at 1245.154 kb on - strand, within MMJJ_RS06745 at 1245.221 kb on + strand, within MMJJ_RS06745 at 1245.223 kb on + strand, within MMJJ_RS06745 at 1245.259 kb on - strand, within MMJJ_RS06745 at 1245.280 kb on - strand, within MMJJ_RS06745 at 1245.295 kb on - strand, within MMJJ_RS06745 at 1245.295 kb on - strand, within MMJJ_RS06745 at 1245.303 kb on - strand, within MMJJ_RS06745 at 1245.303 kb on - strand, within MMJJ_RS06745 at 1245.321 kb on - strand, within MMJJ_RS06745 at 1245.322 kb on + strand, within MMJJ_RS06745 at 1245.391 kb on - strand, within MMJJ_RS06745 at 1245.403 kb on + strand, within MMJJ_RS06745 at 1245.426 kb on - strand, within MMJJ_RS06745 at 1245.479 kb on - strand, within MMJJ_RS06745 at 1245.481 kb on - strand, within MMJJ_RS06745 at 1245.481 kb on - strand, within MMJJ_RS06745 at 1245.481 kb on - strand, within MMJJ_RS06745 at 1245.482 kb on + strand, within MMJJ_RS06745 at 1245.483 kb on - strand, within MMJJ_RS06745 at 1245.559 kb on + strand at 1245.575 kb on + strand at 1245.614 kb on - strand at 1245.614 kb on - strand at 1245.614 kb on - strand at 1245.616 kb on - strand at 1245.616 kb on - strand at 1245.616 kb on - strand at 1245.616 kb on - strand at 1245.617 kb on + strand at 1245.617 kb on + strand at 1245.617 kb on + strand at 1245.617 kb on + strand at 1245.617 kb on + strand at 1245.617 kb on + strand at 1245.617 kb on + strand at 1245.660 kb on - strand at 1245.660 kb on - strand at 1245.661 kb on + strand at 1245.663 kb on + strand at 1245.680 kb on - strand at 1245.683 kb on - strand at 1245.683 kb on - strand at 1245.684 kb on + strand at 1245.684 kb on + strand at 1245.686 kb on - strand at 1245.686 kb on - strand at 1245.686 kb on - strand at 1245.686 kb on - strand at 1245.686 kb on - strand at 1245.686 kb on - strand at 1245.687 kb on + strand at 1245.687 kb on + strand at 1245.687 kb on + strand at 1245.687 kb on + strand at 1245.688 kb on - strand at 1245.690 kb on - strand at 1245.690 kb on - strand at 1245.691 kb on + strand at 1245.697 kb on + strand at 1245.702 kb on - strand at 1245.702 kb on - strand at 1245.710 kb on + strand at 1245.719 kb on - strand at 1245.720 kb on + strand at 1245.720 kb on + strand at 1245.726 kb on - strand at 1245.729 kb on - strand at 1245.729 kb on - strand at 1245.730 kb on + strand at 1245.739 kb on - strand at 1245.739 kb on - strand at 1245.791 kb on - strand at 1245.791 kb on - strand at 1245.970 kb on - strand, within MMJJ_RS06750 at 1246.104 kb on + strand, within MMJJ_RS06750 at 1246.185 kb on + strand at 1246.425 kb on - strand, within MMJJ_RS06750 at 1246.618 kb on - strand, within MMJJ_RS06750 at 1246.723 kb on - strand, within MMJJ_RS06750 at 1246.871 kb on + strand at 1246.998 kb on + strand at 1247.005 kb on - strand at 1247.006 kb on + strand at 1247.008 kb on - strand at 1247.008 kb on - strand at 1247.008 kb on - strand at 1247.010 kb on - strand at 1247.010 kb on - strand at 1247.010 kb on - strand at 1247.010 kb on - strand at 1247.010 kb on - strand at 1247.010 kb on - strand at 1247.010 kb on - strand at 1247.011 kb on + strand at 1247.011 kb on + strand at 1247.011 kb on + strand at 1247.011 kb on + strand at 1247.011 kb on + strand at 1247.014 kb on - strand at 1247.023 kb on - strand at 1247.030 kb on - strand at 1247.030 kb on - strand at 1247.030 kb on - strand at 1247.030 kb on - strand at 1247.030 kb on - strand at 1247.031 kb on + strand at 1247.031 kb on + strand at 1247.052 kb on - strand at 1247.053 kb on + strand at 1247.056 kb on - strand at 1247.056 kb on - strand at 1247.058 kb on - strand at 1247.067 kb on - strand at 1247.067 kb on - strand at 1247.068 kb on + strand at 1247.094 kb on + strand at 1247.094 kb on + strand at 1247.095 kb on - strand at 1247.103 kb on + strand at 1247.122 kb on + strand at 1247.153 kb on + strand at 1247.299 kb on - strand, within MMJJ_RS06755 at 1247.299 kb on - strand, within MMJJ_RS06755 at 1247.300 kb on + strand, within MMJJ_RS06755 at 1247.300 kb on + strand, within MMJJ_RS06755 at 1247.303 kb on - strand, within MMJJ_RS06755 at 1247.303 kb on - strand, within MMJJ_RS06755 at 1247.366 kb on + strand, within MMJJ_RS06755 at 1247.402 kb on + strand, within MMJJ_RS06755 at 1247.402 kb on + strand, within MMJJ_RS06755 at 1247.403 kb on - strand, within MMJJ_RS06755 at 1247.456 kb on + strand, within MMJJ_RS06755 at 1247.480 kb on + strand, within MMJJ_RS06755 at 1247.485 kb on - strand, within MMJJ_RS06755 at 1247.591 kb on - strand at 1247.591 kb on - strand, within MMJJ_RS06755 at 1247.592 kb on + strand, within MMJJ_RS06755 at 1247.598 kb on + strand, within MMJJ_RS06755 at 1247.600 kb on - strand, within MMJJ_RS06755 at 1247.626 kb on - strand, within MMJJ_RS06755 at 1247.663 kb on + strand, within MMJJ_RS06755 at 1247.663 kb on + strand, within MMJJ_RS06755 at 1247.673 kb on - strand, within MMJJ_RS06755 at 1247.673 kb on - strand, within MMJJ_RS06755 at 1247.706 kb on - strand, within MMJJ_RS06755 at 1247.715 kb on - strand, within MMJJ_RS06755 at 1247.716 kb on + strand, within MMJJ_RS06755 at 1247.760 kb on - strand, within MMJJ_RS06755 at 1247.772 kb on - strand, within MMJJ_RS06755 at 1247.772 kb on - strand, within MMJJ_RS06755 at 1247.772 kb on - strand, within MMJJ_RS06755 at 1247.772 kb on - strand, within MMJJ_RS06755 at 1247.773 kb on + strand, within MMJJ_RS06755 at 1247.773 kb on + strand, within MMJJ_RS06755 at 1247.773 kb on + strand, within MMJJ_RS06755 at 1247.773 kb on + strand, within MMJJ_RS06755 at 1247.773 kb on + strand, within MMJJ_RS06755 at 1247.773 kb on + strand, within MMJJ_RS06755 at 1247.774 kb on - strand, within MMJJ_RS06755 at 1247.774 kb on - strand, within MMJJ_RS06755 at 1247.774 kb on - strand, within MMJJ_RS06755 at 1247.775 kb on + strand, within MMJJ_RS06755 at 1247.775 kb on + strand, within MMJJ_RS06755 at 1247.846 kb on + strand, within MMJJ_RS06755 at 1247.852 kb on + strand, within MMJJ_RS06755 at 1247.857 kb on - strand, within MMJJ_RS06755 at 1247.903 kb on - strand, within MMJJ_RS06755 at 1247.904 kb on + strand, within MMJJ_RS06755 at 1247.904 kb on + strand, within MMJJ_RS06755 at 1247.904 kb on + strand, within MMJJ_RS06755 at 1247.905 kb on - strand, within MMJJ_RS06755 at 1247.906 kb on + strand, within MMJJ_RS06755 at 1247.924 kb on - strand, within MMJJ_RS06755 at 1247.925 kb on + strand, within MMJJ_RS06755 at 1247.927 kb on - strand, within MMJJ_RS06755 at 1247.933 kb on - strand, within MMJJ_RS06755 at 1247.983 kb on - strand at 1247.983 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,244,785 + MMJJ_RS06740 0.68 +0.5 1,244,812 + MMJJ_RS06740 0.77 -0.2 1,244,829 + MMJJ_RS06740 0.83 -0.6 1,244,834 + MMJJ_RS06740 0.85 +2.3 1,244,840 - MMJJ_RS06740 0.87 -1.0 1,244,841 + MMJJ_RS06740 0.87 +0.1 1,244,841 + MMJJ_RS06740 0.87 +0.9 1,244,841 + MMJJ_RS06740 0.87 +0.2 1,244,913 - -0.2 1,244,957 - MMJJ_RS06745 0.12 +0.5 1,244,958 + MMJJ_RS06745 0.12 +1.0 1,244,968 + MMJJ_RS06745 0.13 +1.0 1,244,968 + MMJJ_RS06745 0.13 +1.3 1,244,968 + MMJJ_RS06745 0.13 -0.4 1,244,977 - MMJJ_RS06745 0.15 -0.1 1,244,980 - MMJJ_RS06745 0.15 +1.0 1,245,023 + MMJJ_RS06745 0.21 -1.8 1,245,096 - MMJJ_RS06745 0.32 -1.0 1,245,096 - MMJJ_RS06745 0.32 -0.0 1,245,101 - MMJJ_RS06745 0.33 +0.9 1,245,154 - MMJJ_RS06745 0.41 +1.1 1,245,221 + MMJJ_RS06745 0.51 -0.7 1,245,223 + MMJJ_RS06745 0.51 +0.3 1,245,259 - MMJJ_RS06745 0.56 -1.4 1,245,280 - MMJJ_RS06745 0.59 +0.7 1,245,295 - MMJJ_RS06745 0.62 +1.2 1,245,295 - MMJJ_RS06745 0.62 -0.3 1,245,303 - MMJJ_RS06745 0.63 -0.3 1,245,303 - MMJJ_RS06745 0.63 +1.6 1,245,321 - MMJJ_RS06745 0.65 +0.2 1,245,322 + MMJJ_RS06745 0.65 +0.2 1,245,391 - MMJJ_RS06745 0.76 -1.6 1,245,403 + MMJJ_RS06745 0.77 +0.4 1,245,426 - MMJJ_RS06745 0.81 +0.2 1,245,479 - MMJJ_RS06745 0.89 +0.1 1,245,481 - MMJJ_RS06745 0.89 -0.1 1,245,481 - MMJJ_RS06745 0.89 +0.0 1,245,481 - MMJJ_RS06745 0.89 +0.3 1,245,482 + MMJJ_RS06745 0.89 -0.9 1,245,483 - MMJJ_RS06745 0.89 -0.6 1,245,559 + -0.2 1,245,575 + +1.0 1,245,614 - -3.3 1,245,614 - +0.2 1,245,614 - +0.1 1,245,616 - +1.3 1,245,616 - -0.3 1,245,616 - +0.3 1,245,616 - +0.7 1,245,617 + +0.1 1,245,617 + -1.3 1,245,617 + -2.5 1,245,617 + +0.7 1,245,617 + +1.3 1,245,617 + -1.8 1,245,617 + -0.5 1,245,660 - +0.5 1,245,660 - +0.0 1,245,661 + +1.0 1,245,663 + +1.1 1,245,680 - +0.3 1,245,683 - +1.2 1,245,683 - +0.5 1,245,684 + +1.1 1,245,684 + -0.0 1,245,686 - +0.2 1,245,686 - +1.5 1,245,686 - -1.0 1,245,686 - +0.3 1,245,686 - +0.2 1,245,686 - +0.3 1,245,687 + -0.5 1,245,687 + +1.7 1,245,687 + +0.6 1,245,687 + +1.0 1,245,688 - -0.3 1,245,690 - +1.3 1,245,690 - +1.0 1,245,691 + -0.2 1,245,697 + +1.5 1,245,702 - -1.2 1,245,702 - +2.1 1,245,710 + +1.6 1,245,719 - +0.9 1,245,720 + -0.3 1,245,720 + +0.1 1,245,726 - +1.0 1,245,729 - +2.0 1,245,729 - +0.5 1,245,730 + +0.8 1,245,739 - -2.8 1,245,739 - -0.5 1,245,791 - -2.7 1,245,791 - -1.7 1,245,970 - MMJJ_RS06750 0.19 -2.3 1,246,104 + MMJJ_RS06750 0.30 -1.1 1,246,185 + -0.1 1,246,425 - MMJJ_RS06750 0.55 -1.3 1,246,618 - MMJJ_RS06750 0.71 -1.1 1,246,723 - MMJJ_RS06750 0.79 -1.3 1,246,871 + +0.5 1,246,998 + +1.0 1,247,005 - -0.5 1,247,006 + -0.5 1,247,008 - -1.0 1,247,008 - +1.2 1,247,008 - -0.2 1,247,010 - +1.0 1,247,010 - +1.0 1,247,010 - -0.6 1,247,010 - +0.7 1,247,010 - +1.4 1,247,010 - -0.5 1,247,010 - -0.3 1,247,011 + +0.2 1,247,011 + +0.2 1,247,011 + -2.2 1,247,011 + +0.6 1,247,011 + +0.3 1,247,014 - +0.4 1,247,023 - +0.8 1,247,030 - -0.3 1,247,030 - +0.6 1,247,030 - -1.0 1,247,030 - +0.4 1,247,030 - +0.8 1,247,031 + +0.2 1,247,031 + -3.0 1,247,052 - +1.3 1,247,053 + +0.5 1,247,056 - +0.6 1,247,056 - +0.1 1,247,058 - -0.1 1,247,067 - +1.2 1,247,067 - +0.8 1,247,068 + +1.2 1,247,094 + -1.6 1,247,094 + +1.6 1,247,095 - +0.3 1,247,103 + +1.4 1,247,122 + -0.0 1,247,153 + +0.3 1,247,299 - MMJJ_RS06755 0.21 +0.7 1,247,299 - MMJJ_RS06755 0.21 -2.6 1,247,300 + MMJJ_RS06755 0.22 +0.9 1,247,300 + MMJJ_RS06755 0.22 +0.2 1,247,303 - MMJJ_RS06755 0.22 +1.8 1,247,303 - MMJJ_RS06755 0.22 -0.0 1,247,366 + MMJJ_RS06755 0.29 +0.5 1,247,402 + MMJJ_RS06755 0.32 +0.6 1,247,402 + MMJJ_RS06755 0.32 +0.6 1,247,403 - MMJJ_RS06755 0.32 -0.6 1,247,456 + MMJJ_RS06755 0.38 +0.1 1,247,480 + MMJJ_RS06755 0.41 -0.6 1,247,485 - MMJJ_RS06755 0.41 +0.3 1,247,591 - -0.8 1,247,591 - MMJJ_RS06755 0.53 +1.3 1,247,592 + MMJJ_RS06755 0.53 -1.5 1,247,598 + MMJJ_RS06755 0.53 -0.0 1,247,600 - MMJJ_RS06755 0.53 +1.0 1,247,626 - MMJJ_RS06755 0.56 +0.4 1,247,663 + MMJJ_RS06755 0.60 +0.4 1,247,663 + MMJJ_RS06755 0.60 +1.2 1,247,673 - MMJJ_RS06755 0.61 +1.3 1,247,673 - MMJJ_RS06755 0.61 +0.8 1,247,706 - MMJJ_RS06755 0.65 +0.4 1,247,715 - MMJJ_RS06755 0.66 +0.6 1,247,716 + MMJJ_RS06755 0.66 +0.9 1,247,760 - MMJJ_RS06755 0.71 -1.9 1,247,772 - MMJJ_RS06755 0.72 +0.8 1,247,772 - MMJJ_RS06755 0.72 -2.0 1,247,772 - MMJJ_RS06755 0.72 +1.4 1,247,772 - MMJJ_RS06755 0.72 -0.3 1,247,773 + MMJJ_RS06755 0.72 +0.7 1,247,773 + MMJJ_RS06755 0.72 -0.8 1,247,773 + MMJJ_RS06755 0.72 +0.8 1,247,773 + MMJJ_RS06755 0.72 -0.5 1,247,773 + MMJJ_RS06755 0.72 -0.7 1,247,773 + MMJJ_RS06755 0.72 +0.7 1,247,774 - MMJJ_RS06755 0.72 -1.7 1,247,774 - MMJJ_RS06755 0.72 +0.5 1,247,774 - MMJJ_RS06755 0.72 -1.6 1,247,775 + MMJJ_RS06755 0.72 -0.6 1,247,775 + MMJJ_RS06755 0.72 -0.4 1,247,846 + MMJJ_RS06755 0.80 +1.2 1,247,852 + MMJJ_RS06755 0.80 -0.2 1,247,857 - MMJJ_RS06755 0.81 -1.0 1,247,903 - MMJJ_RS06755 0.86 -1.5 1,247,904 + MMJJ_RS06755 0.86 -1.8 1,247,904 + MMJJ_RS06755 0.86 +0.8 1,247,904 + MMJJ_RS06755 0.86 +1.0 1,247,905 - MMJJ_RS06755 0.86 -0.2 1,247,906 + MMJJ_RS06755 0.86 -0.7 1,247,924 - MMJJ_RS06755 0.88 +0.6 1,247,925 + MMJJ_RS06755 0.88 +1.0 1,247,927 - MMJJ_RS06755 0.88 +0.5 1,247,933 - MMJJ_RS06755 0.89 +0.0 1,247,983 - +0.4 1,247,983 - +1.0
Or see this region's nucleotide sequence