Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06475 and MMJJ_RS06480 are separated by 8 nucleotides MMJJ_RS06480 and MMJJ_RS06485 are separated by 366 nucleotides MMJJ_RS06485 and MMJJ_RS06490 are separated by 147 nucleotides
MMJJ_RS06475: MMJJ_RS06475 - AAA family ATPase, at 1,194,769 to 1,195,314
_RS06475
MMJJ_RS06480: MMJJ_RS06480 - hypothetical protein, at 1,195,323 to 1,195,775
_RS06480
MMJJ_RS06485: MMJJ_RS06485 - phosphoglycerate kinase, at 1,196,142 to 1,197,386
_RS06485
MMJJ_RS06490: MMJJ_RS06490 - methanogenesis marker 2 protein, at 1,197,534 to 1,198,505
_RS06490
Position (kb)
1196
1197
1198 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1195.148 kb on - strand, within MMJJ_RS06475 at 1195.213 kb on - strand, within MMJJ_RS06475 at 1195.238 kb on + strand, within MMJJ_RS06475 at 1195.238 kb on + strand, within MMJJ_RS06475 at 1195.238 kb on + strand, within MMJJ_RS06475 at 1195.267 kb on - strand at 1195.272 kb on - strand at 1195.273 kb on + strand at 1195.307 kb on + strand at 1195.307 kb on + strand at 1195.310 kb on + strand at 1195.310 kb on + strand at 1195.312 kb on - strand at 1195.313 kb on + strand at 1195.314 kb on - strand at 1195.324 kb on - strand at 1195.324 kb on - strand at 1195.325 kb on + strand at 1195.325 kb on + strand at 1195.325 kb on + strand at 1195.360 kb on - strand at 1195.360 kb on - strand at 1195.360 kb on - strand at 1195.361 kb on + strand at 1195.361 kb on + strand at 1195.361 kb on + strand at 1195.445 kb on + strand, within MMJJ_RS06480 at 1195.470 kb on - strand, within MMJJ_RS06480 at 1195.509 kb on - strand, within MMJJ_RS06480 at 1195.511 kb on - strand, within MMJJ_RS06480 at 1195.511 kb on - strand, within MMJJ_RS06480 at 1195.511 kb on - strand, within MMJJ_RS06480 at 1195.511 kb on - strand, within MMJJ_RS06480 at 1195.512 kb on + strand, within MMJJ_RS06480 at 1195.524 kb on - strand, within MMJJ_RS06480 at 1195.524 kb on - strand, within MMJJ_RS06480 at 1195.527 kb on - strand, within MMJJ_RS06480 at 1195.528 kb on + strand, within MMJJ_RS06480 at 1195.530 kb on + strand, within MMJJ_RS06480 at 1195.562 kb on - strand, within MMJJ_RS06480 at 1195.595 kb on - strand, within MMJJ_RS06480 at 1195.596 kb on + strand, within MMJJ_RS06480 at 1195.604 kb on - strand, within MMJJ_RS06480 at 1195.628 kb on - strand, within MMJJ_RS06480 at 1195.670 kb on + strand, within MMJJ_RS06480 at 1195.678 kb on - strand, within MMJJ_RS06480 at 1195.690 kb on + strand, within MMJJ_RS06480 at 1195.692 kb on - strand, within MMJJ_RS06480 at 1195.692 kb on - strand, within MMJJ_RS06480 at 1195.693 kb on + strand, within MMJJ_RS06480 at 1195.693 kb on + strand, within MMJJ_RS06480 at 1195.699 kb on - strand, within MMJJ_RS06480 at 1195.699 kb on - strand, within MMJJ_RS06480 at 1195.700 kb on + strand, within MMJJ_RS06480 at 1195.700 kb on + strand, within MMJJ_RS06480 at 1195.700 kb on + strand, within MMJJ_RS06480 at 1195.700 kb on + strand, within MMJJ_RS06480 at 1195.700 kb on + strand, within MMJJ_RS06480 at 1195.713 kb on - strand, within MMJJ_RS06480 at 1195.739 kb on - strand at 1195.753 kb on + strand at 1195.753 kb on + strand at 1195.808 kb on - strand at 1195.808 kb on - strand at 1195.809 kb on + strand at 1195.809 kb on + strand at 1195.818 kb on - strand at 1195.818 kb on - strand at 1195.819 kb on + strand at 1195.819 kb on + strand at 1195.821 kb on + strand at 1195.823 kb on - strand at 1195.823 kb on - strand at 1195.823 kb on - strand at 1195.823 kb on - strand at 1195.823 kb on - strand at 1195.824 kb on + strand at 1195.829 kb on - strand at 1195.829 kb on - strand at 1195.830 kb on + strand at 1195.830 kb on + strand at 1195.831 kb on - strand at 1195.831 kb on - strand at 1195.832 kb on + strand at 1195.832 kb on + strand at 1195.832 kb on + strand at 1195.832 kb on + strand at 1195.833 kb on - strand at 1195.834 kb on + strand at 1195.842 kb on - strand at 1195.843 kb on + strand at 1195.883 kb on - strand at 1195.884 kb on + strand at 1195.888 kb on + strand at 1195.890 kb on - strand at 1195.890 kb on - strand at 1195.890 kb on - strand at 1195.890 kb on - strand at 1195.890 kb on - strand at 1195.890 kb on - strand at 1195.891 kb on + strand at 1195.891 kb on + strand at 1195.891 kb on + strand at 1195.891 kb on + strand at 1195.891 kb on + strand at 1195.891 kb on + strand at 1195.891 kb on + strand at 1195.891 kb on + strand at 1195.891 kb on + strand at 1195.891 kb on + strand at 1195.892 kb on - strand at 1195.892 kb on - strand at 1195.892 kb on - strand at 1195.892 kb on - strand at 1195.892 kb on - strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.893 kb on + strand at 1195.918 kb on + strand at 1195.918 kb on + strand at 1195.918 kb on + strand at 1195.921 kb on - strand at 1195.922 kb on + strand at 1195.922 kb on + strand at 1195.922 kb on + strand at 1195.922 kb on - strand at 1195.924 kb on - strand at 1195.925 kb on + strand at 1195.934 kb on - strand at 1195.970 kb on - strand at 1195.970 kb on - strand at 1195.971 kb on + strand at 1195.982 kb on + strand at 1196.007 kb on + strand at 1196.007 kb on + strand at 1196.009 kb on - strand at 1196.010 kb on + strand at 1196.014 kb on - strand at 1196.016 kb on - strand at 1196.016 kb on - strand at 1196.017 kb on + strand at 1196.017 kb on + strand at 1196.017 kb on + strand at 1196.017 kb on + strand at 1196.017 kb on + strand at 1196.020 kb on - strand at 1196.021 kb on + strand at 1196.021 kb on + strand at 1196.021 kb on + strand at 1196.023 kb on - strand at 1196.024 kb on + strand at 1196.041 kb on - strand at 1196.042 kb on + strand at 1196.048 kb on + strand at 1196.051 kb on + strand at 1196.054 kb on + strand at 1196.067 kb on + strand at 1196.067 kb on + strand at 1196.067 kb on + strand at 1196.067 kb on + strand at 1196.072 kb on + strand at 1196.072 kb on + strand at 1196.075 kb on - strand at 1196.096 kb on + strand at 1196.205 kb on + strand at 1196.247 kb on + strand at 1196.270 kb on - strand, within MMJJ_RS06485 at 1196.306 kb on + strand, within MMJJ_RS06485 at 1196.390 kb on + strand, within MMJJ_RS06485 at 1196.411 kb on + strand, within MMJJ_RS06485 at 1196.558 kb on - strand, within MMJJ_RS06485 at 1196.708 kb on - strand, within MMJJ_RS06485 at 1196.708 kb on - strand, within MMJJ_RS06485 at 1196.998 kb on - strand, within MMJJ_RS06485 at 1197.343 kb on - strand at 1197.377 kb on - strand at 1197.377 kb on - strand at 1197.445 kb on - strand at 1197.459 kb on - strand at 1197.459 kb on - strand at 1197.459 kb on - strand at 1197.459 kb on - strand at 1197.459 kb on - strand at 1197.460 kb on + strand at 1197.460 kb on + strand at 1197.460 kb on + strand at 1197.461 kb on - strand at 1197.461 kb on - strand at 1197.462 kb on + strand at 1197.462 kb on + strand at 1197.463 kb on - strand at 1197.465 kb on - strand at 1197.465 kb on - strand at 1197.466 kb on + strand at 1197.480 kb on - strand at 1197.480 kb on - strand at 1197.647 kb on - strand, within MMJJ_RS06490 at 1197.656 kb on + strand, within MMJJ_RS06490 at 1197.836 kb on - strand, within MMJJ_RS06490 at 1197.847 kb on - strand, within MMJJ_RS06490 at 1197.962 kb on + strand at 1198.119 kb on - strand at 1198.181 kb on - strand, within MMJJ_RS06490 at 1198.189 kb on - strand, within MMJJ_RS06490 at 1198.265 kb on - strand at 1198.333 kb on - strand, within MMJJ_RS06490
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,195,148 - MMJJ_RS06475 0.69 -0.3 1,195,213 - MMJJ_RS06475 0.81 +0.9 1,195,238 + MMJJ_RS06475 0.86 +0.0 1,195,238 + MMJJ_RS06475 0.86 +0.4 1,195,238 + MMJJ_RS06475 0.86 +1.0 1,195,267 - +0.2 1,195,272 - +0.8 1,195,273 + +0.2 1,195,307 + -0.6 1,195,307 + +0.5 1,195,310 + -0.2 1,195,310 + +0.6 1,195,312 - +0.4 1,195,313 + +0.1 1,195,314 - +0.1 1,195,324 - +0.6 1,195,324 - -1.2 1,195,325 + +0.4 1,195,325 + +0.7 1,195,325 + +1.5 1,195,360 - +1.6 1,195,360 - -0.9 1,195,360 - -0.2 1,195,361 + +0.0 1,195,361 + -0.5 1,195,361 + -1.3 1,195,445 + MMJJ_RS06480 0.27 -1.3 1,195,470 - MMJJ_RS06480 0.32 +0.4 1,195,509 - MMJJ_RS06480 0.41 -0.5 1,195,511 - MMJJ_RS06480 0.42 -1.1 1,195,511 - MMJJ_RS06480 0.42 +0.6 1,195,511 - MMJJ_RS06480 0.42 -0.2 1,195,511 - MMJJ_RS06480 0.42 -0.3 1,195,512 + MMJJ_RS06480 0.42 +0.8 1,195,524 - MMJJ_RS06480 0.44 -2.0 1,195,524 - MMJJ_RS06480 0.44 -0.8 1,195,527 - MMJJ_RS06480 0.45 -0.0 1,195,528 + MMJJ_RS06480 0.45 -1.0 1,195,530 + MMJJ_RS06480 0.46 +1.7 1,195,562 - MMJJ_RS06480 0.53 +0.0 1,195,595 - MMJJ_RS06480 0.60 +0.5 1,195,596 + MMJJ_RS06480 0.60 +0.1 1,195,604 - MMJJ_RS06480 0.62 -0.3 1,195,628 - MMJJ_RS06480 0.67 -0.6 1,195,670 + MMJJ_RS06480 0.77 -0.5 1,195,678 - MMJJ_RS06480 0.78 -0.3 1,195,690 + MMJJ_RS06480 0.81 -2.3 1,195,692 - MMJJ_RS06480 0.81 +0.1 1,195,692 - MMJJ_RS06480 0.81 -1.9 1,195,693 + MMJJ_RS06480 0.82 -1.0 1,195,693 + MMJJ_RS06480 0.82 +0.6 1,195,699 - MMJJ_RS06480 0.83 -0.4 1,195,699 - MMJJ_RS06480 0.83 -0.0 1,195,700 + MMJJ_RS06480 0.83 +0.1 1,195,700 + MMJJ_RS06480 0.83 -1.9 1,195,700 + MMJJ_RS06480 0.83 -1.9 1,195,700 + MMJJ_RS06480 0.83 -0.7 1,195,700 + MMJJ_RS06480 0.83 -0.9 1,195,713 - MMJJ_RS06480 0.86 -0.7 1,195,739 - -1.2 1,195,753 + +0.3 1,195,753 + +0.2 1,195,808 - -0.1 1,195,808 - +0.7 1,195,809 + -1.4 1,195,809 + -0.9 1,195,818 - +0.1 1,195,818 - +0.8 1,195,819 + -2.5 1,195,819 + +0.1 1,195,821 + -2.0 1,195,823 - -0.1 1,195,823 - -0.5 1,195,823 - -0.5 1,195,823 - -0.8 1,195,823 - -0.2 1,195,824 + -0.6 1,195,829 - +0.4 1,195,829 - -0.3 1,195,830 + -0.7 1,195,830 + -0.3 1,195,831 - -0.8 1,195,831 - -1.3 1,195,832 + -0.1 1,195,832 + +0.1 1,195,832 + +0.4 1,195,832 + -0.0 1,195,833 - -1.9 1,195,834 + +0.3 1,195,842 - -1.0 1,195,843 + +0.1 1,195,883 - +0.3 1,195,884 + -2.9 1,195,888 + +0.3 1,195,890 - +1.2 1,195,890 - -3.5 1,195,890 - +0.7 1,195,890 - -0.6 1,195,890 - +0.3 1,195,890 - +1.0 1,195,891 + +1.2 1,195,891 + +0.4 1,195,891 + +0.4 1,195,891 + +0.1 1,195,891 + +0.7 1,195,891 + +0.4 1,195,891 + +0.5 1,195,891 + -1.0 1,195,891 + +0.8 1,195,891 + +0.2 1,195,892 - +0.9 1,195,892 - +1.0 1,195,892 - +0.0 1,195,892 - +0.5 1,195,892 - +1.0 1,195,893 + +0.6 1,195,893 + -2.3 1,195,893 + +0.5 1,195,893 + +0.5 1,195,893 + +0.1 1,195,893 + -1.1 1,195,893 + +1.0 1,195,893 + +0.3 1,195,893 + -0.3 1,195,893 + +1.0 1,195,893 + +0.3 1,195,893 + +0.9 1,195,918 + -0.2 1,195,918 + -0.3 1,195,918 + +0.5 1,195,921 - -1.1 1,195,922 + -0.3 1,195,922 + +0.3 1,195,922 + +0.3 1,195,922 - -0.4 1,195,924 - +0.0 1,195,925 + +0.8 1,195,934 - -0.5 1,195,970 - +0.4 1,195,970 - -0.1 1,195,971 + -0.0 1,195,982 + +0.3 1,196,007 + +0.6 1,196,007 + +0.7 1,196,009 - +1.4 1,196,010 + +0.4 1,196,014 - +2.2 1,196,016 - +1.1 1,196,016 - -2.4 1,196,017 + +0.2 1,196,017 + +0.7 1,196,017 + +0.8 1,196,017 + -0.9 1,196,017 + +0.7 1,196,020 - -0.1 1,196,021 + +0.2 1,196,021 + -0.7 1,196,021 + +0.8 1,196,023 - -1.0 1,196,024 + +1.0 1,196,041 - -1.6 1,196,042 + -0.5 1,196,048 + +0.4 1,196,051 + -0.9 1,196,054 + -1.9 1,196,067 + -1.1 1,196,067 + -1.1 1,196,067 + -2.4 1,196,067 + -1.1 1,196,072 + -1.9 1,196,072 + -1.8 1,196,075 - -2.6 1,196,096 + -0.9 1,196,205 + +0.4 1,196,247 + -2.5 1,196,270 - MMJJ_RS06485 0.10 -2.8 1,196,306 + MMJJ_RS06485 0.13 -2.3 1,196,390 + MMJJ_RS06485 0.20 -0.8 1,196,411 + MMJJ_RS06485 0.22 -1.9 1,196,558 - MMJJ_RS06485 0.33 -2.2 1,196,708 - MMJJ_RS06485 0.45 -1.8 1,196,708 - MMJJ_RS06485 0.45 -0.9 1,196,998 - MMJJ_RS06485 0.69 -2.6 1,197,343 - -1.6 1,197,377 - +0.6 1,197,377 - -0.8 1,197,445 - +0.7 1,197,459 - +1.1 1,197,459 - +1.2 1,197,459 - -0.1 1,197,459 - +0.7 1,197,459 - +0.7 1,197,460 + +0.6 1,197,460 + +0.3 1,197,460 + -2.1 1,197,461 - -1.5 1,197,461 - -1.5 1,197,462 + -2.0 1,197,462 + -2.3 1,197,463 - -1.9 1,197,465 - -2.4 1,197,465 - -2.2 1,197,466 + -2.5 1,197,480 - -0.9 1,197,480 - -1.2 1,197,647 - MMJJ_RS06490 0.12 -3.6 1,197,656 + MMJJ_RS06490 0.13 -1.2 1,197,836 - MMJJ_RS06490 0.31 -2.9 1,197,847 - MMJJ_RS06490 0.32 -1.2 1,197,962 + -1.4 1,198,119 - -1.6 1,198,181 - MMJJ_RS06490 0.67 -0.2 1,198,189 - MMJJ_RS06490 0.67 -1.6 1,198,265 - -0.2 1,198,333 - MMJJ_RS06490 0.82 -1.8
Or see this region's nucleotide sequence