Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06255 and MMJJ_RS09355 are separated by 15 nucleotides MMJJ_RS09355 and MMJJ_RS06260 are separated by 185 nucleotides MMJJ_RS06260 and MMJJ_RS06265 are separated by 8 nucleotides
MMJJ_RS06255: MMJJ_RS06255 - tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3, at 1,160,643 to 1,162,265
_RS06255
MMJJ_RS09355: MMJJ_RS09355 - hypothetical protein, at 1,162,281 to 1,162,436
_RS09355
MMJJ_RS06260: MMJJ_RS06260 - 6-carboxyhexanoate--CoA ligase, at 1,162,622 to 1,163,356
_RS06260
MMJJ_RS06265: MMJJ_RS06265 - 8-amino-7-oxononanoate synthase, at 1,163,365 to 1,164,477
_RS06265
Position (kb)
1162
1163
1164 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1161.630 kb on + strand, within MMJJ_RS06255 at 1161.656 kb on + strand, within MMJJ_RS06255 at 1161.656 kb on + strand, within MMJJ_RS06255 at 1161.663 kb on + strand, within MMJJ_RS06255 at 1161.752 kb on - strand, within MMJJ_RS06255 at 1161.781 kb on + strand, within MMJJ_RS06255 at 1161.805 kb on - strand, within MMJJ_RS06255 at 1161.813 kb on + strand, within MMJJ_RS06255 at 1161.818 kb on - strand, within MMJJ_RS06255 at 1161.819 kb on + strand, within MMJJ_RS06255 at 1161.845 kb on - strand, within MMJJ_RS06255 at 1161.850 kb on + strand, within MMJJ_RS06255 at 1161.850 kb on + strand, within MMJJ_RS06255 at 1161.929 kb on + strand, within MMJJ_RS06255 at 1162.193 kb on + strand at 1162.209 kb on - strand at 1162.242 kb on - strand at 1162.283 kb on + strand at 1162.294 kb on + strand at 1162.304 kb on - strand, within MMJJ_RS09355 at 1162.308 kb on - strand, within MMJJ_RS09355 at 1162.308 kb on - strand, within MMJJ_RS09355 at 1162.309 kb on + strand, within MMJJ_RS09355 at 1162.311 kb on - strand, within MMJJ_RS09355 at 1162.324 kb on - strand, within MMJJ_RS09355 at 1162.339 kb on + strand, within MMJJ_RS09355 at 1162.367 kb on - strand, within MMJJ_RS09355 at 1162.367 kb on - strand, within MMJJ_RS09355 at 1162.432 kb on + strand at 1162.476 kb on - strand at 1162.478 kb on - strand at 1162.479 kb on + strand at 1162.480 kb on - strand at 1162.480 kb on - strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.482 kb on - strand at 1162.482 kb on - strand at 1162.483 kb on + strand at 1162.483 kb on + strand at 1162.499 kb on - strand at 1162.572 kb on + strand at 1162.591 kb on - strand at 1162.592 kb on + strand at 1162.617 kb on + strand at 1162.765 kb on - strand, within MMJJ_RS06260 at 1162.800 kb on - strand, within MMJJ_RS06260 at 1162.805 kb on - strand, within MMJJ_RS06260 at 1162.876 kb on - strand, within MMJJ_RS06260 at 1162.877 kb on + strand, within MMJJ_RS06260 at 1162.877 kb on + strand, within MMJJ_RS06260 at 1162.960 kb on - strand, within MMJJ_RS06260 at 1163.080 kb on + strand, within MMJJ_RS06260 at 1163.182 kb on - strand, within MMJJ_RS06260 at 1163.201 kb on - strand, within MMJJ_RS06260 at 1163.213 kb on - strand, within MMJJ_RS06260 at 1163.214 kb on + strand, within MMJJ_RS06260 at 1163.225 kb on + strand, within MMJJ_RS06260 at 1163.239 kb on - strand, within MMJJ_RS06260 at 1163.240 kb on + strand, within MMJJ_RS06260 at 1163.247 kb on - strand, within MMJJ_RS06260 at 1163.247 kb on - strand, within MMJJ_RS06260 at 1163.284 kb on + strand at 1163.335 kb on - strand at 1163.351 kb on - strand at 1163.352 kb on + strand at 1163.352 kb on + strand at 1163.354 kb on - strand at 1163.355 kb on + strand at 1163.365 kb on + strand at 1163.506 kb on + strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.513 kb on + strand, within MMJJ_RS06265 at 1163.531 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.635 kb on - strand, within MMJJ_RS06265 at 1163.660 kb on - strand, within MMJJ_RS06265 at 1163.660 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.670 kb on + strand, within MMJJ_RS06265 at 1163.683 kb on + strand, within MMJJ_RS06265 at 1163.714 kb on - strand, within MMJJ_RS06265 at 1163.730 kb on + strand, within MMJJ_RS06265 at 1163.742 kb on + strand, within MMJJ_RS06265 at 1163.782 kb on + strand, within MMJJ_RS06265 at 1163.798 kb on - strand, within MMJJ_RS06265 at 1163.799 kb on + strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.914 kb on - strand, within MMJJ_RS06265 at 1163.914 kb on - strand, within MMJJ_RS06265 at 1163.928 kb on + strand, within MMJJ_RS06265 at 1163.928 kb on + strand, within MMJJ_RS06265 at 1163.961 kb on + strand, within MMJJ_RS06265 at 1164.013 kb on + strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.066 kb on + strand, within MMJJ_RS06265 at 1164.092 kb on + strand, within MMJJ_RS06265 at 1164.118 kb on + strand, within MMJJ_RS06265 at 1164.118 kb on + strand, within MMJJ_RS06265 at 1164.160 kb on + strand, within MMJJ_RS06265 at 1164.274 kb on + strand, within MMJJ_RS06265
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,161,630 + MMJJ_RS06255 0.61 -2.1 1,161,656 + MMJJ_RS06255 0.62 -1.4 1,161,656 + MMJJ_RS06255 0.62 -1.2 1,161,663 + MMJJ_RS06255 0.63 -3.4 1,161,752 - MMJJ_RS06255 0.68 -2.0 1,161,781 + MMJJ_RS06255 0.70 -1.6 1,161,805 - MMJJ_RS06255 0.72 -2.3 1,161,813 + MMJJ_RS06255 0.72 -1.2 1,161,818 - MMJJ_RS06255 0.72 -2.4 1,161,819 + MMJJ_RS06255 0.72 -2.2 1,161,845 - MMJJ_RS06255 0.74 -1.6 1,161,850 + MMJJ_RS06255 0.74 -1.0 1,161,850 + MMJJ_RS06255 0.74 -0.4 1,161,929 + MMJJ_RS06255 0.79 -2.5 1,162,193 + -3.7 1,162,209 - -2.2 1,162,242 - -3.3 1,162,283 + +0.7 1,162,294 + +0.8 1,162,304 - MMJJ_RS09355 0.15 +0.9 1,162,308 - MMJJ_RS09355 0.17 +0.5 1,162,308 - MMJJ_RS09355 0.17 -0.1 1,162,309 + MMJJ_RS09355 0.18 -0.7 1,162,311 - MMJJ_RS09355 0.19 -0.4 1,162,324 - MMJJ_RS09355 0.28 +0.7 1,162,339 + MMJJ_RS09355 0.37 +0.7 1,162,367 - MMJJ_RS09355 0.55 +1.2 1,162,367 - MMJJ_RS09355 0.55 +0.1 1,162,432 + -0.2 1,162,476 - -0.3 1,162,478 - +0.8 1,162,479 + +1.4 1,162,480 - +0.0 1,162,480 - -0.6 1,162,481 + +1.3 1,162,481 + +0.3 1,162,481 + +1.6 1,162,481 + +0.4 1,162,482 - +1.1 1,162,482 - +1.0 1,162,483 + +0.4 1,162,483 + -0.1 1,162,499 - +1.1 1,162,572 + +0.9 1,162,591 - -0.0 1,162,592 + +0.2 1,162,617 + -1.0 1,162,765 - MMJJ_RS06260 0.19 -1.7 1,162,800 - MMJJ_RS06260 0.24 -2.0 1,162,805 - MMJJ_RS06260 0.25 -4.3 1,162,876 - MMJJ_RS06260 0.35 -3.1 1,162,877 + MMJJ_RS06260 0.35 -2.2 1,162,877 + MMJJ_RS06260 0.35 -1.2 1,162,960 - MMJJ_RS06260 0.46 -2.1 1,163,080 + MMJJ_RS06260 0.62 -2.4 1,163,182 - MMJJ_RS06260 0.76 -1.7 1,163,201 - MMJJ_RS06260 0.79 -3.3 1,163,213 - MMJJ_RS06260 0.80 -2.2 1,163,214 + MMJJ_RS06260 0.81 -4.0 1,163,225 + MMJJ_RS06260 0.82 -3.2 1,163,239 - MMJJ_RS06260 0.84 -2.0 1,163,240 + MMJJ_RS06260 0.84 -2.7 1,163,247 - MMJJ_RS06260 0.85 -1.9 1,163,247 - MMJJ_RS06260 0.85 -1.5 1,163,284 + -2.7 1,163,335 - -2.7 1,163,351 - -2.2 1,163,352 + -2.4 1,163,352 + -1.3 1,163,354 - +0.4 1,163,355 + +0.5 1,163,365 + -1.3 1,163,506 + MMJJ_RS06265 0.13 -0.9 1,163,512 - MMJJ_RS06265 0.13 -1.3 1,163,512 - MMJJ_RS06265 0.13 -3.0 1,163,512 - MMJJ_RS06265 0.13 -2.7 1,163,512 - MMJJ_RS06265 0.13 -1.7 1,163,512 - MMJJ_RS06265 0.13 -0.1 1,163,513 + MMJJ_RS06265 0.13 -2.2 1,163,531 + MMJJ_RS06265 0.15 -4.4 1,163,574 + MMJJ_RS06265 0.19 -4.4 1,163,574 + MMJJ_RS06265 0.19 -2.1 1,163,574 + MMJJ_RS06265 0.19 -0.9 1,163,635 - MMJJ_RS06265 0.24 -2.4 1,163,660 - MMJJ_RS06265 0.27 -2.1 1,163,660 - MMJJ_RS06265 0.27 -2.7 1,163,667 - MMJJ_RS06265 0.27 -3.0 1,163,667 - MMJJ_RS06265 0.27 -1.1 1,163,667 - MMJJ_RS06265 0.27 -0.5 1,163,669 - MMJJ_RS06265 0.27 -0.7 1,163,669 - MMJJ_RS06265 0.27 -1.8 1,163,669 - MMJJ_RS06265 0.27 -2.6 1,163,669 - MMJJ_RS06265 0.27 -2.1 1,163,670 + MMJJ_RS06265 0.27 -1.7 1,163,683 + MMJJ_RS06265 0.29 -2.0 1,163,714 - MMJJ_RS06265 0.31 -3.4 1,163,730 + MMJJ_RS06265 0.33 -2.9 1,163,742 + MMJJ_RS06265 0.34 -0.7 1,163,782 + MMJJ_RS06265 0.37 -2.6 1,163,798 - MMJJ_RS06265 0.39 -2.1 1,163,799 + MMJJ_RS06265 0.39 -1.5 1,163,843 - MMJJ_RS06265 0.43 -0.2 1,163,843 - MMJJ_RS06265 0.43 -2.0 1,163,843 - MMJJ_RS06265 0.43 -2.0 1,163,843 - MMJJ_RS06265 0.43 -3.4 1,163,844 + MMJJ_RS06265 0.43 -2.9 1,163,844 + MMJJ_RS06265 0.43 -2.2 1,163,844 + MMJJ_RS06265 0.43 -0.3 1,163,914 - MMJJ_RS06265 0.49 -2.5 1,163,914 - MMJJ_RS06265 0.49 -2.8 1,163,928 + MMJJ_RS06265 0.51 -2.4 1,163,928 + MMJJ_RS06265 0.51 -2.5 1,163,961 + MMJJ_RS06265 0.54 -2.1 1,164,013 + MMJJ_RS06265 0.58 -1.4 1,164,065 - MMJJ_RS06265 0.63 -0.7 1,164,065 - MMJJ_RS06265 0.63 -3.7 1,164,065 - MMJJ_RS06265 0.63 -1.8 1,164,065 - MMJJ_RS06265 0.63 -2.2 1,164,066 + MMJJ_RS06265 0.63 -2.6 1,164,092 + MMJJ_RS06265 0.65 -2.7 1,164,118 + MMJJ_RS06265 0.68 -1.4 1,164,118 + MMJJ_RS06265 0.68 -1.8 1,164,160 + MMJJ_RS06265 0.71 -3.4 1,164,274 + MMJJ_RS06265 0.82 +6.4
Or see this region's nucleotide sequence