Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06245 and MMJJ_RS06250 are separated by 128 nucleotides MMJJ_RS06250 and MMJJ_RS06255 are separated by 130 nucleotides MMJJ_RS06255 and MMJJ_RS09355 are separated by 15 nucleotides MMJJ_RS09355 and MMJJ_RS06260 are separated by 185 nucleotides
MMJJ_RS06245: MMJJ_RS06245 - 30S ribosomal protein S15, at 1,159,413 to 1,159,868
_RS06245
MMJJ_RS06250: MMJJ_RS06250 - nicotinamide-nucleotide adenylyltransferase, at 1,159,997 to 1,160,512
_RS06250
MMJJ_RS06255: MMJJ_RS06255 - tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3, at 1,160,643 to 1,162,265
_RS06255
MMJJ_RS09355: MMJJ_RS09355 - hypothetical protein, at 1,162,281 to 1,162,436
_RS09355
MMJJ_RS06260: MMJJ_RS06260 - 6-carboxyhexanoate--CoA ligase, at 1,162,622 to 1,163,356
_RS06260
Position (kb)
1160
1161
1162
1163 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1159.857 kb on - strand at 1159.958 kb on - strand at 1159.958 kb on - strand at 1159.962 kb on - strand at 1159.976 kb on - strand at 1159.987 kb on - strand at 1159.988 kb on + strand at 1160.063 kb on - strand, within MMJJ_RS06250 at 1160.088 kb on + strand at 1160.152 kb on - strand, within MMJJ_RS06250 at 1160.182 kb on + strand, within MMJJ_RS06250 at 1160.198 kb on - strand, within MMJJ_RS06250 at 1160.236 kb on - strand at 1160.281 kb on - strand at 1160.281 kb on - strand at 1160.283 kb on - strand, within MMJJ_RS06250 at 1160.283 kb on - strand, within MMJJ_RS06250 at 1160.288 kb on - strand, within MMJJ_RS06250 at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.546 kb on + strand at 1160.551 kb on - strand at 1160.552 kb on + strand at 1160.556 kb on - strand at 1160.557 kb on + strand at 1160.580 kb on + strand at 1160.583 kb on + strand at 1160.586 kb on - strand at 1160.586 kb on - strand at 1160.587 kb on + strand at 1160.593 kb on + strand at 1160.593 kb on + strand at 1160.594 kb on - strand at 1160.595 kb on + strand at 1160.605 kb on - strand at 1160.606 kb on + strand at 1160.607 kb on - strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.609 kb on - strand at 1160.609 kb on - strand at 1160.609 kb on - strand at 1160.610 kb on + strand at 1160.610 kb on + strand at 1160.613 kb on + strand at 1160.620 kb on + strand at 1160.666 kb on + strand at 1160.689 kb on - strand at 1160.690 kb on + strand at 1160.973 kb on + strand, within MMJJ_RS06255 at 1160.998 kb on + strand, within MMJJ_RS06255 at 1161.036 kb on - strand, within MMJJ_RS06255 at 1161.037 kb on + strand, within MMJJ_RS06255 at 1161.052 kb on + strand, within MMJJ_RS06255 at 1161.112 kb on - strand, within MMJJ_RS06255 at 1161.366 kb on + strand at 1161.422 kb on - strand at 1161.465 kb on - strand, within MMJJ_RS06255 at 1161.466 kb on + strand at 1161.603 kb on - strand, within MMJJ_RS06255 at 1161.604 kb on + strand, within MMJJ_RS06255 at 1161.611 kb on + strand, within MMJJ_RS06255 at 1161.630 kb on + strand, within MMJJ_RS06255 at 1161.656 kb on + strand, within MMJJ_RS06255 at 1161.656 kb on + strand, within MMJJ_RS06255 at 1161.663 kb on + strand, within MMJJ_RS06255 at 1161.752 kb on - strand, within MMJJ_RS06255 at 1161.781 kb on + strand, within MMJJ_RS06255 at 1161.805 kb on - strand, within MMJJ_RS06255 at 1161.813 kb on + strand, within MMJJ_RS06255 at 1161.818 kb on - strand, within MMJJ_RS06255 at 1161.819 kb on + strand, within MMJJ_RS06255 at 1161.845 kb on - strand, within MMJJ_RS06255 at 1161.850 kb on + strand, within MMJJ_RS06255 at 1161.850 kb on + strand, within MMJJ_RS06255 at 1161.929 kb on + strand, within MMJJ_RS06255 at 1162.193 kb on + strand at 1162.209 kb on - strand at 1162.242 kb on - strand at 1162.283 kb on + strand at 1162.294 kb on + strand at 1162.304 kb on - strand, within MMJJ_RS09355 at 1162.308 kb on - strand, within MMJJ_RS09355 at 1162.308 kb on - strand, within MMJJ_RS09355 at 1162.309 kb on + strand, within MMJJ_RS09355 at 1162.311 kb on - strand, within MMJJ_RS09355 at 1162.324 kb on - strand, within MMJJ_RS09355 at 1162.339 kb on + strand, within MMJJ_RS09355 at 1162.367 kb on - strand, within MMJJ_RS09355 at 1162.367 kb on - strand, within MMJJ_RS09355 at 1162.432 kb on + strand at 1162.476 kb on - strand at 1162.478 kb on - strand at 1162.479 kb on + strand at 1162.480 kb on - strand at 1162.480 kb on - strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.482 kb on - strand at 1162.482 kb on - strand at 1162.483 kb on + strand at 1162.483 kb on + strand at 1162.499 kb on - strand at 1162.572 kb on + strand at 1162.591 kb on - strand at 1162.592 kb on + strand at 1162.617 kb on + strand at 1162.765 kb on - strand, within MMJJ_RS06260 at 1162.800 kb on - strand, within MMJJ_RS06260 at 1162.805 kb on - strand, within MMJJ_RS06260 at 1162.876 kb on - strand, within MMJJ_RS06260 at 1162.877 kb on + strand, within MMJJ_RS06260 at 1162.877 kb on + strand, within MMJJ_RS06260 at 1162.960 kb on - strand, within MMJJ_RS06260 at 1163.080 kb on + strand, within MMJJ_RS06260 at 1163.182 kb on - strand, within MMJJ_RS06260 at 1163.201 kb on - strand, within MMJJ_RS06260 at 1163.213 kb on - strand, within MMJJ_RS06260 at 1163.214 kb on + strand, within MMJJ_RS06260 at 1163.225 kb on + strand, within MMJJ_RS06260 at 1163.239 kb on - strand, within MMJJ_RS06260 at 1163.240 kb on + strand, within MMJJ_RS06260 at 1163.247 kb on - strand, within MMJJ_RS06260 at 1163.247 kb on - strand, within MMJJ_RS06260
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,159,857 - -0.2 1,159,958 - -1.0 1,159,958 - -1.6 1,159,962 - -0.1 1,159,976 - +0.1 1,159,987 - +0.4 1,159,988 + +1.5 1,160,063 - MMJJ_RS06250 0.13 -1.6 1,160,088 + +0.4 1,160,152 - MMJJ_RS06250 0.30 +0.1 1,160,182 + MMJJ_RS06250 0.36 -2.1 1,160,198 - MMJJ_RS06250 0.39 -1.4 1,160,236 - +1.4 1,160,281 - -1.0 1,160,281 - -0.2 1,160,283 - MMJJ_RS06250 0.55 -2.0 1,160,283 - MMJJ_RS06250 0.55 -0.4 1,160,288 - MMJJ_RS06250 0.56 -2.3 1,160,545 - -2.0 1,160,545 - -1.0 1,160,545 - +0.5 1,160,545 - -4.4 1,160,545 - -3.4 1,160,546 + -1.0 1,160,551 - -1.0 1,160,552 + -2.2 1,160,556 - -2.7 1,160,557 + -2.6 1,160,580 + -1.6 1,160,583 + +0.2 1,160,586 - -1.2 1,160,586 - -0.4 1,160,587 + -1.6 1,160,593 + -0.6 1,160,593 + +0.4 1,160,594 - -1.1 1,160,595 + -0.6 1,160,605 - -1.0 1,160,606 + -0.2 1,160,607 - -2.0 1,160,608 + +0.3 1,160,608 + -3.0 1,160,608 + -1.3 1,160,608 + -0.3 1,160,608 + -0.6 1,160,609 - -1.4 1,160,609 - -1.4 1,160,609 - -1.6 1,160,610 + -1.6 1,160,610 + -2.1 1,160,613 + -2.2 1,160,620 + -1.7 1,160,666 + +0.4 1,160,689 - -2.7 1,160,690 + -1.4 1,160,973 + MMJJ_RS06255 0.20 -2.4 1,160,998 + MMJJ_RS06255 0.22 -1.6 1,161,036 - MMJJ_RS06255 0.24 -3.0 1,161,037 + MMJJ_RS06255 0.24 -1.0 1,161,052 + MMJJ_RS06255 0.25 -2.6 1,161,112 - MMJJ_RS06255 0.29 -1.6 1,161,366 + -1.0 1,161,422 - -0.2 1,161,465 - MMJJ_RS06255 0.51 -3.2 1,161,466 + +0.4 1,161,603 - MMJJ_RS06255 0.59 +0.0 1,161,604 + MMJJ_RS06255 0.59 -1.2 1,161,611 + MMJJ_RS06255 0.60 -0.4 1,161,630 + MMJJ_RS06255 0.61 -2.1 1,161,656 + MMJJ_RS06255 0.62 -1.4 1,161,656 + MMJJ_RS06255 0.62 -1.2 1,161,663 + MMJJ_RS06255 0.63 -3.4 1,161,752 - MMJJ_RS06255 0.68 -2.0 1,161,781 + MMJJ_RS06255 0.70 -1.6 1,161,805 - MMJJ_RS06255 0.72 -2.3 1,161,813 + MMJJ_RS06255 0.72 -1.2 1,161,818 - MMJJ_RS06255 0.72 -2.4 1,161,819 + MMJJ_RS06255 0.72 -2.2 1,161,845 - MMJJ_RS06255 0.74 -1.6 1,161,850 + MMJJ_RS06255 0.74 -1.0 1,161,850 + MMJJ_RS06255 0.74 -0.4 1,161,929 + MMJJ_RS06255 0.79 -2.5 1,162,193 + -3.7 1,162,209 - -2.2 1,162,242 - -3.3 1,162,283 + +0.7 1,162,294 + +0.8 1,162,304 - MMJJ_RS09355 0.15 +0.9 1,162,308 - MMJJ_RS09355 0.17 +0.5 1,162,308 - MMJJ_RS09355 0.17 -0.1 1,162,309 + MMJJ_RS09355 0.18 -0.7 1,162,311 - MMJJ_RS09355 0.19 -0.4 1,162,324 - MMJJ_RS09355 0.28 +0.7 1,162,339 + MMJJ_RS09355 0.37 +0.7 1,162,367 - MMJJ_RS09355 0.55 +1.2 1,162,367 - MMJJ_RS09355 0.55 +0.1 1,162,432 + -0.2 1,162,476 - -0.3 1,162,478 - +0.8 1,162,479 + +1.4 1,162,480 - +0.0 1,162,480 - -0.6 1,162,481 + +1.3 1,162,481 + +0.3 1,162,481 + +1.6 1,162,481 + +0.4 1,162,482 - +1.1 1,162,482 - +1.0 1,162,483 + +0.4 1,162,483 + -0.1 1,162,499 - +1.1 1,162,572 + +0.9 1,162,591 - -0.0 1,162,592 + +0.2 1,162,617 + -1.0 1,162,765 - MMJJ_RS06260 0.19 -1.7 1,162,800 - MMJJ_RS06260 0.24 -2.0 1,162,805 - MMJJ_RS06260 0.25 -4.3 1,162,876 - MMJJ_RS06260 0.35 -3.1 1,162,877 + MMJJ_RS06260 0.35 -2.2 1,162,877 + MMJJ_RS06260 0.35 -1.2 1,162,960 - MMJJ_RS06260 0.46 -2.1 1,163,080 + MMJJ_RS06260 0.62 -2.4 1,163,182 - MMJJ_RS06260 0.76 -1.7 1,163,201 - MMJJ_RS06260 0.79 -3.3 1,163,213 - MMJJ_RS06260 0.80 -2.2 1,163,214 + MMJJ_RS06260 0.81 -4.0 1,163,225 + MMJJ_RS06260 0.82 -3.2 1,163,239 - MMJJ_RS06260 0.84 -2.0 1,163,240 + MMJJ_RS06260 0.84 -2.7 1,163,247 - MMJJ_RS06260 0.85 -1.9 1,163,247 - MMJJ_RS06260 0.85 -1.5
Or see this region's nucleotide sequence