Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06180 and MMJJ_RS06185 are separated by 36 nucleotides MMJJ_RS06185 and MMJJ_RS06190 are separated by 0 nucleotides MMJJ_RS06190 and MMJJ_RS06195 are separated by 83 nucleotides
MMJJ_RS06180: MMJJ_RS06180 - tryptophan--tRNA ligase, at 1,146,276 to 1,147,352
_RS06180
MMJJ_RS06185: MMJJ_RS06185 - cobalamin biosynthesis protein, at 1,147,389 to 1,148,360
_RS06185
MMJJ_RS06190: MMJJ_RS06190 - 7-cyano-7-deazaguanine synthase, at 1,148,361 to 1,149,146
_RS06190
MMJJ_RS06195: MMJJ_RS06195 - glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit, at 1,149,230 to 1,150,330
_RS06195
Position (kb)
1147
1148
1149 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1146.562 kb on - strand, within MMJJ_RS06180 at 1146.563 kb on + strand at 1146.563 kb on + strand, within MMJJ_RS06180 at 1146.564 kb on - strand, within MMJJ_RS06180 at 1146.564 kb on - strand, within MMJJ_RS06180 at 1146.564 kb on - strand, within MMJJ_RS06180 at 1146.565 kb on + strand at 1146.658 kb on + strand, within MMJJ_RS06180 at 1146.697 kb on + strand at 1146.842 kb on - strand, within MMJJ_RS06180 at 1147.174 kb on + strand, within MMJJ_RS06180 at 1147.394 kb on + strand at 1147.405 kb on + strand at 1147.441 kb on - strand at 1147.441 kb on - strand at 1147.461 kb on - strand at 1147.490 kb on + strand, within MMJJ_RS06185 at 1147.505 kb on + strand, within MMJJ_RS06185 at 1147.532 kb on - strand, within MMJJ_RS06185 at 1147.535 kb on + strand, within MMJJ_RS06185 at 1147.563 kb on - strand, within MMJJ_RS06185 at 1147.591 kb on - strand, within MMJJ_RS06185 at 1147.636 kb on - strand, within MMJJ_RS06185 at 1147.636 kb on - strand, within MMJJ_RS06185 at 1147.687 kb on - strand, within MMJJ_RS06185 at 1147.739 kb on - strand, within MMJJ_RS06185 at 1147.817 kb on + strand, within MMJJ_RS06185 at 1147.829 kb on + strand, within MMJJ_RS06185 at 1148.080 kb on - strand, within MMJJ_RS06185 at 1148.165 kb on + strand at 1148.175 kb on - strand, within MMJJ_RS06185 at 1148.192 kb on + strand, within MMJJ_RS06185 at 1148.195 kb on - strand at 1148.195 kb on - strand, within MMJJ_RS06185 at 1148.358 kb on - strand at 1148.359 kb on + strand at 1148.359 kb on + strand at 1148.362 kb on - strand at 1148.363 kb on + strand at 1148.373 kb on - strand at 1148.374 kb on + strand at 1148.374 kb on + strand at 1148.382 kb on - strand at 1148.382 kb on - strand at 1148.382 kb on - strand at 1148.382 kb on - strand at 1148.382 kb on - strand at 1148.382 kb on - strand at 1148.383 kb on + strand at 1148.383 kb on + strand at 1148.383 kb on + strand at 1148.384 kb on - strand at 1148.385 kb on + strand at 1148.408 kb on - strand at 1148.409 kb on + strand at 1148.412 kb on - strand at 1148.424 kb on - strand at 1148.514 kb on - strand, within MMJJ_RS06190 at 1148.516 kb on - strand, within MMJJ_RS06190 at 1148.520 kb on + strand, within MMJJ_RS06190 at 1148.569 kb on + strand, within MMJJ_RS06190 at 1148.643 kb on + strand, within MMJJ_RS06190 at 1148.653 kb on + strand, within MMJJ_RS06190 at 1148.653 kb on + strand, within MMJJ_RS06190 at 1148.659 kb on - strand, within MMJJ_RS06190 at 1148.664 kb on - strand, within MMJJ_RS06190 at 1148.667 kb on + strand, within MMJJ_RS06190 at 1148.734 kb on - strand, within MMJJ_RS06190 at 1148.751 kb on + strand, within MMJJ_RS06190 at 1148.751 kb on + strand, within MMJJ_RS06190 at 1148.769 kb on - strand, within MMJJ_RS06190 at 1148.770 kb on + strand, within MMJJ_RS06190 at 1148.783 kb on - strand, within MMJJ_RS06190 at 1148.784 kb on + strand, within MMJJ_RS06190 at 1148.802 kb on - strand, within MMJJ_RS06190 at 1148.802 kb on - strand, within MMJJ_RS06190 at 1148.803 kb on + strand, within MMJJ_RS06190 at 1148.813 kb on - strand, within MMJJ_RS06190 at 1148.814 kb on + strand at 1148.814 kb on + strand, within MMJJ_RS06190 at 1148.814 kb on + strand, within MMJJ_RS06190 at 1148.850 kb on - strand, within MMJJ_RS06190 at 1148.850 kb on - strand, within MMJJ_RS06190 at 1148.850 kb on - strand, within MMJJ_RS06190 at 1148.852 kb on - strand, within MMJJ_RS06190 at 1148.852 kb on - strand, within MMJJ_RS06190 at 1148.853 kb on + strand, within MMJJ_RS06190 at 1148.853 kb on + strand, within MMJJ_RS06190 at 1148.853 kb on + strand, within MMJJ_RS06190 at 1148.853 kb on + strand, within MMJJ_RS06190 at 1148.854 kb on - strand, within MMJJ_RS06190 at 1148.854 kb on - strand, within MMJJ_RS06190 at 1148.854 kb on - strand, within MMJJ_RS06190 at 1148.854 kb on - strand, within MMJJ_RS06190 at 1148.855 kb on + strand, within MMJJ_RS06190 at 1148.855 kb on + strand, within MMJJ_RS06190 at 1148.855 kb on + strand, within MMJJ_RS06190 at 1148.855 kb on + strand, within MMJJ_RS06190 at 1148.858 kb on - strand, within MMJJ_RS06190 at 1148.858 kb on - strand, within MMJJ_RS06190 at 1148.871 kb on + strand, within MMJJ_RS06190 at 1148.871 kb on + strand, within MMJJ_RS06190 at 1148.874 kb on - strand, within MMJJ_RS06190 at 1148.907 kb on - strand, within MMJJ_RS06190 at 1148.907 kb on - strand, within MMJJ_RS06190 at 1148.908 kb on + strand, within MMJJ_RS06190 at 1148.915 kb on - strand, within MMJJ_RS06190 at 1148.916 kb on + strand, within MMJJ_RS06190 at 1148.919 kb on + strand, within MMJJ_RS06190 at 1148.921 kb on - strand, within MMJJ_RS06190 at 1148.921 kb on - strand, within MMJJ_RS06190 at 1148.921 kb on - strand, within MMJJ_RS06190 at 1148.925 kb on - strand, within MMJJ_RS06190 at 1148.925 kb on - strand, within MMJJ_RS06190 at 1148.926 kb on + strand, within MMJJ_RS06190 at 1148.979 kb on - strand, within MMJJ_RS06190 at 1148.980 kb on + strand, within MMJJ_RS06190 at 1148.992 kb on + strand, within MMJJ_RS06190 at 1149.085 kb on - strand at 1149.085 kb on - strand at 1149.086 kb on + strand at 1149.087 kb on - strand at 1149.087 kb on - strand at 1149.087 kb on - strand at 1149.088 kb on + strand at 1149.088 kb on + strand at 1149.088 kb on + strand at 1149.088 kb on + strand at 1149.088 kb on + strand at 1149.090 kb on - strand at 1149.090 kb on - strand at 1149.090 kb on - strand at 1149.090 kb on - strand at 1149.090 kb on - strand at 1149.090 kb on - strand at 1149.091 kb on + strand at 1149.091 kb on + strand at 1149.125 kb on - strand at 1149.131 kb on - strand at 1149.140 kb on - strand at 1149.170 kb on - strand at 1149.181 kb on - strand at 1149.182 kb on + strand at 1149.185 kb on - strand at 1149.192 kb on + strand at 1149.212 kb on - strand at 1149.212 kb on - strand at 1149.212 kb on - strand at 1149.212 kb on - strand at 1149.213 kb on + strand at 1149.215 kb on - strand at 1149.215 kb on - strand at 1149.216 kb on + strand at 1149.216 kb on + strand at 1149.218 kb on + strand at 1149.251 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,146,562 - MMJJ_RS06180 0.27 -2.7 1,146,563 + -0.2 1,146,563 + MMJJ_RS06180 0.27 -1.8 1,146,564 - MMJJ_RS06180 0.27 -2.6 1,146,564 - MMJJ_RS06180 0.27 +0.4 1,146,564 - MMJJ_RS06180 0.27 -1.2 1,146,565 + +0.4 1,146,658 + MMJJ_RS06180 0.35 +0.4 1,146,697 + -0.6 1,146,842 - MMJJ_RS06180 0.53 -2.2 1,147,174 + MMJJ_RS06180 0.83 -2.7 1,147,394 + -2.0 1,147,405 + -2.5 1,147,441 - -2.6 1,147,441 - -1.2 1,147,461 - -2.0 1,147,490 + MMJJ_RS06185 0.10 -1.8 1,147,505 + MMJJ_RS06185 0.12 +1.0 1,147,532 - MMJJ_RS06185 0.15 -3.1 1,147,535 + MMJJ_RS06185 0.15 -1.6 1,147,563 - MMJJ_RS06185 0.18 -3.0 1,147,591 - MMJJ_RS06185 0.21 -2.5 1,147,636 - MMJJ_RS06185 0.25 -2.0 1,147,636 - MMJJ_RS06185 0.25 -2.6 1,147,687 - MMJJ_RS06185 0.31 -2.4 1,147,739 - MMJJ_RS06185 0.36 -1.6 1,147,817 + MMJJ_RS06185 0.44 -1.6 1,147,829 + MMJJ_RS06185 0.45 -1.8 1,148,080 - MMJJ_RS06185 0.71 -2.2 1,148,165 + -1.0 1,148,175 - MMJJ_RS06185 0.81 -1.2 1,148,192 + MMJJ_RS06185 0.83 -3.5 1,148,195 - +1.4 1,148,195 - MMJJ_RS06185 0.83 -0.7 1,148,358 - -1.2 1,148,359 + -0.6 1,148,359 + +1.9 1,148,362 - +1.3 1,148,363 + +0.2 1,148,373 - -1.6 1,148,374 + +1.6 1,148,374 + -0.6 1,148,382 - +0.9 1,148,382 - +0.4 1,148,382 - -0.5 1,148,382 - +0.1 1,148,382 - +0.4 1,148,382 - -2.2 1,148,383 + -0.9 1,148,383 + -0.5 1,148,383 + -1.1 1,148,384 - +0.3 1,148,385 + -0.7 1,148,408 - -0.6 1,148,409 + -1.7 1,148,412 - +0.7 1,148,424 - +0.7 1,148,514 - MMJJ_RS06190 0.19 +0.3 1,148,516 - MMJJ_RS06190 0.20 -0.1 1,148,520 + MMJJ_RS06190 0.20 -0.1 1,148,569 + MMJJ_RS06190 0.26 +0.6 1,148,643 + MMJJ_RS06190 0.36 +0.1 1,148,653 + MMJJ_RS06190 0.37 -0.0 1,148,653 + MMJJ_RS06190 0.37 +0.2 1,148,659 - MMJJ_RS06190 0.38 +0.5 1,148,664 - MMJJ_RS06190 0.39 +0.3 1,148,667 + MMJJ_RS06190 0.39 +0.1 1,148,734 - MMJJ_RS06190 0.47 +1.1 1,148,751 + MMJJ_RS06190 0.50 -0.9 1,148,751 + MMJJ_RS06190 0.50 +0.2 1,148,769 - MMJJ_RS06190 0.52 +1.5 1,148,770 + MMJJ_RS06190 0.52 -1.0 1,148,783 - MMJJ_RS06190 0.54 +0.1 1,148,784 + MMJJ_RS06190 0.54 -0.5 1,148,802 - MMJJ_RS06190 0.56 -2.1 1,148,802 - MMJJ_RS06190 0.56 +0.1 1,148,803 + MMJJ_RS06190 0.56 +0.2 1,148,813 - MMJJ_RS06190 0.58 -0.6 1,148,814 + +4.5 1,148,814 + MMJJ_RS06190 0.58 +0.7 1,148,814 + MMJJ_RS06190 0.58 +0.2 1,148,850 - MMJJ_RS06190 0.62 +0.4 1,148,850 - MMJJ_RS06190 0.62 -2.2 1,148,850 - MMJJ_RS06190 0.62 -0.0 1,148,852 - MMJJ_RS06190 0.62 +0.6 1,148,852 - MMJJ_RS06190 0.62 +1.9 1,148,853 + MMJJ_RS06190 0.63 +0.8 1,148,853 + MMJJ_RS06190 0.63 -1.4 1,148,853 + MMJJ_RS06190 0.63 -0.4 1,148,853 + MMJJ_RS06190 0.63 -0.2 1,148,854 - MMJJ_RS06190 0.63 -0.4 1,148,854 - MMJJ_RS06190 0.63 -0.7 1,148,854 - MMJJ_RS06190 0.63 +0.2 1,148,854 - MMJJ_RS06190 0.63 -1.3 1,148,855 + MMJJ_RS06190 0.63 -0.2 1,148,855 + MMJJ_RS06190 0.63 +0.6 1,148,855 + MMJJ_RS06190 0.63 +1.2 1,148,855 + MMJJ_RS06190 0.63 +1.3 1,148,858 - MMJJ_RS06190 0.63 +0.6 1,148,858 - MMJJ_RS06190 0.63 +1.4 1,148,871 + MMJJ_RS06190 0.65 +0.3 1,148,871 + MMJJ_RS06190 0.65 +0.4 1,148,874 - MMJJ_RS06190 0.65 +0.2 1,148,907 - MMJJ_RS06190 0.69 -0.7 1,148,907 - MMJJ_RS06190 0.69 +1.1 1,148,908 + MMJJ_RS06190 0.70 -0.0 1,148,915 - MMJJ_RS06190 0.70 -0.3 1,148,916 + MMJJ_RS06190 0.71 +0.8 1,148,919 + MMJJ_RS06190 0.71 -0.3 1,148,921 - MMJJ_RS06190 0.71 +0.8 1,148,921 - MMJJ_RS06190 0.71 -0.6 1,148,921 - MMJJ_RS06190 0.71 +1.1 1,148,925 - MMJJ_RS06190 0.72 +0.7 1,148,925 - MMJJ_RS06190 0.72 -0.2 1,148,926 + MMJJ_RS06190 0.72 +0.1 1,148,979 - MMJJ_RS06190 0.79 +0.5 1,148,980 + MMJJ_RS06190 0.79 -0.1 1,148,992 + MMJJ_RS06190 0.80 +0.9 1,149,085 - +0.6 1,149,085 - +0.0 1,149,086 + -0.1 1,149,087 - +0.0 1,149,087 - -0.9 1,149,087 - +0.8 1,149,088 + +0.9 1,149,088 + +0.4 1,149,088 + -0.6 1,149,088 + +0.4 1,149,088 + +0.6 1,149,090 - -0.2 1,149,090 - +0.7 1,149,090 - +0.4 1,149,090 - +1.0 1,149,090 - +0.1 1,149,090 - +1.3 1,149,091 + +0.3 1,149,091 + +0.8 1,149,125 - +1.7 1,149,131 - -1.1 1,149,140 - +1.1 1,149,170 - -0.4 1,149,181 - +1.8 1,149,182 + +1.0 1,149,185 - -2.4 1,149,192 + +0.1 1,149,212 - +0.3 1,149,212 - +2.2 1,149,212 - +0.7 1,149,212 - +0.8 1,149,213 + +0.3 1,149,215 - +0.9 1,149,215 - +0.1 1,149,216 + +0.0 1,149,216 + +0.3 1,149,218 + +1.3 1,149,251 + -0.8
Or see this region's nucleotide sequence