Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06165 and MMJJ_RS06170 are separated by 58 nucleotides MMJJ_RS06170 and MMJJ_RS06175 are separated by 11 nucleotides MMJJ_RS06175 and MMJJ_RS06180 are separated by 27 nucleotides
MMJJ_RS06165: MMJJ_RS06165 - archaeosine synthase subunit alpha, at 1,143,114 to 1,144,730
_RS06165
MMJJ_RS06170: MMJJ_RS06170 - hypothetical protein, at 1,144,789 to 1,145,331
_RS06170
MMJJ_RS06175: MMJJ_RS06175 - DUF1743 domain-containing protein, at 1,145,343 to 1,146,248
_RS06175
MMJJ_RS06180: MMJJ_RS06180 - tryptophan--tRNA ligase, at 1,146,276 to 1,147,352
_RS06180
Position (kb)
1145
1146
1147 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1144.396 kb on - strand, within MMJJ_RS06165 at 1144.396 kb on - strand, within MMJJ_RS06165 at 1144.397 kb on + strand, within MMJJ_RS06165 at 1144.402 kb on - strand, within MMJJ_RS06165 at 1144.403 kb on + strand, within MMJJ_RS06165 at 1144.430 kb on + strand, within MMJJ_RS06165 at 1144.489 kb on - strand, within MMJJ_RS06165 at 1144.531 kb on - strand, within MMJJ_RS06165 at 1144.545 kb on + strand, within MMJJ_RS06165 at 1144.564 kb on - strand, within MMJJ_RS06165 at 1144.564 kb on - strand, within MMJJ_RS06165 at 1144.618 kb on - strand at 1144.619 kb on + strand at 1144.623 kb on + strand at 1144.713 kb on + strand at 1144.713 kb on + strand at 1144.728 kb on - strand at 1144.741 kb on + strand at 1144.745 kb on + strand at 1144.799 kb on + strand at 1144.838 kb on - strand at 1144.891 kb on - strand, within MMJJ_RS06170 at 1144.903 kb on - strand, within MMJJ_RS06170 at 1144.903 kb on - strand, within MMJJ_RS06170 at 1144.904 kb on + strand, within MMJJ_RS06170 at 1144.904 kb on + strand, within MMJJ_RS06170 at 1144.904 kb on + strand, within MMJJ_RS06170 at 1144.904 kb on + strand, within MMJJ_RS06170 at 1144.913 kb on - strand at 1144.913 kb on - strand, within MMJJ_RS06170 at 1144.914 kb on + strand, within MMJJ_RS06170 at 1144.959 kb on + strand, within MMJJ_RS06170 at 1144.982 kb on - strand, within MMJJ_RS06170 at 1145.002 kb on - strand, within MMJJ_RS06170 at 1145.002 kb on - strand, within MMJJ_RS06170 at 1145.003 kb on + strand, within MMJJ_RS06170 at 1145.003 kb on + strand, within MMJJ_RS06170 at 1145.016 kb on + strand, within MMJJ_RS06170 at 1145.046 kb on + strand, within MMJJ_RS06170 at 1145.127 kb on - strand, within MMJJ_RS06170 at 1145.145 kb on - strand, within MMJJ_RS06170 at 1145.147 kb on - strand at 1145.147 kb on - strand, within MMJJ_RS06170 at 1145.147 kb on - strand, within MMJJ_RS06170 at 1145.147 kb on - strand, within MMJJ_RS06170 at 1145.148 kb on + strand, within MMJJ_RS06170 at 1145.148 kb on + strand, within MMJJ_RS06170 at 1145.148 kb on + strand, within MMJJ_RS06170 at 1145.148 kb on + strand, within MMJJ_RS06170 at 1145.148 kb on + strand, within MMJJ_RS06170 at 1145.148 kb on + strand, within MMJJ_RS06170 at 1145.148 kb on + strand, within MMJJ_RS06170 at 1145.148 kb on + strand, within MMJJ_RS06170 at 1145.149 kb on - strand, within MMJJ_RS06170 at 1145.149 kb on - strand, within MMJJ_RS06170 at 1145.149 kb on - strand, within MMJJ_RS06170 at 1145.149 kb on - strand, within MMJJ_RS06170 at 1145.149 kb on - strand, within MMJJ_RS06170 at 1145.149 kb on - strand, within MMJJ_RS06170 at 1145.149 kb on - strand, within MMJJ_RS06170 at 1145.149 kb on - strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.150 kb on + strand, within MMJJ_RS06170 at 1145.155 kb on + strand, within MMJJ_RS06170 at 1145.155 kb on + strand at 1145.263 kb on - strand, within MMJJ_RS06170 at 1145.264 kb on + strand, within MMJJ_RS06170 at 1145.301 kb on - strand at 1145.309 kb on + strand at 1145.309 kb on + strand at 1145.330 kb on + strand at 1145.330 kb on + strand at 1145.507 kb on - strand, within MMJJ_RS06175 at 1145.678 kb on + strand at 1145.824 kb on - strand, within MMJJ_RS06175 at 1145.962 kb on + strand, within MMJJ_RS06175 at 1145.962 kb on + strand, within MMJJ_RS06175 at 1146.045 kb on + strand, within MMJJ_RS06175 at 1146.185 kb on + strand at 1146.562 kb on - strand, within MMJJ_RS06180 at 1146.563 kb on + strand at 1146.563 kb on + strand, within MMJJ_RS06180 at 1146.564 kb on - strand, within MMJJ_RS06180 at 1146.564 kb on - strand, within MMJJ_RS06180 at 1146.564 kb on - strand, within MMJJ_RS06180 at 1146.565 kb on + strand at 1146.658 kb on + strand, within MMJJ_RS06180 at 1146.697 kb on + strand at 1146.842 kb on - strand, within MMJJ_RS06180 at 1147.174 kb on + strand, within MMJJ_RS06180
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,144,396 - MMJJ_RS06165 0.79 -1.7 1,144,396 - MMJJ_RS06165 0.79 -2.4 1,144,397 + MMJJ_RS06165 0.79 -2.7 1,144,402 - MMJJ_RS06165 0.80 -0.7 1,144,403 + MMJJ_RS06165 0.80 -2.0 1,144,430 + MMJJ_RS06165 0.81 -0.4 1,144,489 - MMJJ_RS06165 0.85 -0.2 1,144,531 - MMJJ_RS06165 0.88 -1.2 1,144,545 + MMJJ_RS06165 0.88 +0.2 1,144,564 - MMJJ_RS06165 0.90 -1.0 1,144,564 - MMJJ_RS06165 0.90 -0.2 1,144,618 - -1.9 1,144,619 + -1.5 1,144,623 + -2.7 1,144,713 + -1.3 1,144,713 + -3.5 1,144,728 - -0.5 1,144,741 + -2.9 1,144,745 + -0.5 1,144,799 + -1.5 1,144,838 - -3.1 1,144,891 - MMJJ_RS06170 0.19 -0.8 1,144,903 - MMJJ_RS06170 0.21 -1.2 1,144,903 - MMJJ_RS06170 0.21 -2.0 1,144,904 + MMJJ_RS06170 0.21 -1.4 1,144,904 + MMJJ_RS06170 0.21 -1.1 1,144,904 + MMJJ_RS06170 0.21 -1.5 1,144,904 + MMJJ_RS06170 0.21 -1.2 1,144,913 - -0.2 1,144,913 - MMJJ_RS06170 0.23 -1.7 1,144,914 + MMJJ_RS06170 0.23 -2.8 1,144,959 + MMJJ_RS06170 0.31 -0.5 1,144,982 - MMJJ_RS06170 0.36 -2.4 1,145,002 - MMJJ_RS06170 0.39 -1.1 1,145,002 - MMJJ_RS06170 0.39 -1.0 1,145,003 + MMJJ_RS06170 0.39 +0.3 1,145,003 + MMJJ_RS06170 0.39 -3.5 1,145,016 + MMJJ_RS06170 0.42 -1.0 1,145,046 + MMJJ_RS06170 0.47 -1.6 1,145,127 - MMJJ_RS06170 0.62 -2.5 1,145,145 - MMJJ_RS06170 0.66 -1.7 1,145,147 - -0.2 1,145,147 - MMJJ_RS06170 0.66 -0.5 1,145,147 - MMJJ_RS06170 0.66 -0.7 1,145,147 - MMJJ_RS06170 0.66 -3.3 1,145,148 + MMJJ_RS06170 0.66 -2.1 1,145,148 + MMJJ_RS06170 0.66 -1.4 1,145,148 + MMJJ_RS06170 0.66 -2.7 1,145,148 + MMJJ_RS06170 0.66 -2.2 1,145,148 + MMJJ_RS06170 0.66 -0.9 1,145,148 + MMJJ_RS06170 0.66 -1.7 1,145,148 + MMJJ_RS06170 0.66 -2.7 1,145,148 + MMJJ_RS06170 0.66 -1.2 1,145,149 - MMJJ_RS06170 0.66 -2.1 1,145,149 - MMJJ_RS06170 0.66 -2.1 1,145,149 - MMJJ_RS06170 0.66 +0.8 1,145,149 - MMJJ_RS06170 0.66 -1.2 1,145,149 - MMJJ_RS06170 0.66 -1.2 1,145,149 - MMJJ_RS06170 0.66 -3.0 1,145,149 - MMJJ_RS06170 0.66 -1.2 1,145,149 - MMJJ_RS06170 0.66 +0.3 1,145,150 + MMJJ_RS06170 0.66 -0.5 1,145,150 + MMJJ_RS06170 0.66 -2.7 1,145,150 + MMJJ_RS06170 0.66 -0.7 1,145,150 + MMJJ_RS06170 0.66 -2.7 1,145,150 + MMJJ_RS06170 0.66 -0.7 1,145,150 + MMJJ_RS06170 0.66 -1.5 1,145,150 + MMJJ_RS06170 0.66 -3.3 1,145,150 + MMJJ_RS06170 0.66 -3.0 1,145,150 + MMJJ_RS06170 0.66 -2.7 1,145,150 + MMJJ_RS06170 0.66 -1.2 1,145,150 + MMJJ_RS06170 0.66 -4.1 1,145,150 + MMJJ_RS06170 0.66 -2.4 1,145,155 + MMJJ_RS06170 0.67 -0.8 1,145,155 + +0.8 1,145,263 - MMJJ_RS06170 0.87 -0.7 1,145,264 + MMJJ_RS06170 0.87 -1.1 1,145,301 - -1.0 1,145,309 + -1.8 1,145,309 + -0.7 1,145,330 + -2.5 1,145,330 + -2.2 1,145,507 - MMJJ_RS06175 0.18 +0.3 1,145,678 + -0.7 1,145,824 - MMJJ_RS06175 0.53 -2.5 1,145,962 + MMJJ_RS06175 0.68 -1.0 1,145,962 + MMJJ_RS06175 0.68 -1.5 1,146,045 + MMJJ_RS06175 0.77 -2.6 1,146,185 + -2.0 1,146,562 - MMJJ_RS06180 0.27 -2.7 1,146,563 + -0.2 1,146,563 + MMJJ_RS06180 0.27 -1.8 1,146,564 - MMJJ_RS06180 0.27 -2.6 1,146,564 - MMJJ_RS06180 0.27 +0.4 1,146,564 - MMJJ_RS06180 0.27 -1.2 1,146,565 + +0.4 1,146,658 + MMJJ_RS06180 0.35 +0.4 1,146,697 + -0.6 1,146,842 - MMJJ_RS06180 0.53 -2.2 1,147,174 + MMJJ_RS06180 0.83 -2.7
Or see this region's nucleotide sequence