Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS06080

Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS06070 and MMJJ_RS06075 are separated by 35 nucleotidesMMJJ_RS06075 and MMJJ_RS06080 are separated by 111 nucleotidesMMJJ_RS06080 and MMJJ_RS06085 are separated by 23 nucleotidesMMJJ_RS06085 and MMJJ_RS06090 are separated by 19 nucleotides MMJJ_RS06070: MMJJ_RS06070 - histidine--tRNA ligase, at 1,127,552 to 1,128,802 _RS06070 MMJJ_RS06075: MMJJ_RS06075 - DNA-binding protein Alba, at 1,128,838 to 1,129,107 _RS06075 MMJJ_RS06080: MMJJ_RS06080 - serine/threonine protein kinase, at 1,129,219 to 1,129,896 _RS06080 MMJJ_RS06085: MMJJ_RS06085 - proteasome assembly chaperone family protein, at 1,129,920 to 1,130,672 _RS06085 MMJJ_RS06090: MMJJ_RS06090 - hypothetical protein, at 1,130,692 to 1,131,366 _RS06090 Position (kb) 1129 1130Strain fitness (log2 ratio) -2 -1 0 1 2at 1128.351 kb on + strand, within MMJJ_RS06070at 1128.353 kb on - strand, within MMJJ_RS06070at 1128.787 kb on - strandat 1128.787 kb on - strandat 1128.844 kb on + strandat 1128.868 kb on - strand, within MMJJ_RS06075at 1128.898 kb on - strand, within MMJJ_RS06075at 1128.898 kb on - strand, within MMJJ_RS06075at 1128.898 kb on - strand, within MMJJ_RS06075at 1128.898 kb on - strand, within MMJJ_RS06075at 1128.898 kb on - strand, within MMJJ_RS06075at 1128.898 kb on - strand, within MMJJ_RS06075at 1128.898 kb on - strand, within MMJJ_RS06075at 1128.898 kb on - strand, within MMJJ_RS06075at 1128.898 kb on - strand, within MMJJ_RS06075at 1128.898 kb on - strand, within MMJJ_RS06075at 1128.899 kb on + strand, within MMJJ_RS06075at 1128.899 kb on + strand, within MMJJ_RS06075at 1128.899 kb on + strand, within MMJJ_RS06075at 1128.899 kb on + strand, within MMJJ_RS06075at 1128.899 kb on + strand, within MMJJ_RS06075at 1128.899 kb on + strand, within MMJJ_RS06075at 1128.899 kb on + strand, within MMJJ_RS06075at 1128.899 kb on + strand, within MMJJ_RS06075at 1128.900 kb on - strand, within MMJJ_RS06075at 1128.901 kb on + strand, within MMJJ_RS06075at 1129.009 kb on - strand, within MMJJ_RS06075at 1129.039 kb on + strand, within MMJJ_RS06075at 1129.048 kb on + strand, within MMJJ_RS06075at 1129.064 kb on - strand, within MMJJ_RS06075at 1129.064 kb on - strand, within MMJJ_RS06075at 1129.073 kb on + strand, within MMJJ_RS06075at 1129.091 kb on - strandat 1129.092 kb on + strandat 1129.098 kb on - strandat 1129.131 kb on - strandat 1129.132 kb on + strandat 1129.145 kb on - strandat 1129.145 kb on - strandat 1129.145 kb on - strandat 1129.145 kb on - strandat 1129.146 kb on + strandat 1129.146 kb on + strandat 1129.147 kb on - strandat 1129.147 kb on - strandat 1129.147 kb on - strandat 1129.148 kb on + strandat 1129.148 kb on + strandat 1129.149 kb on - strandat 1129.149 kb on - strandat 1129.149 kb on - strandat 1129.150 kb on + strandat 1129.162 kb on + strandat 1129.195 kb on + strandat 1129.195 kb on + strandat 1129.257 kb on - strandat 1129.312 kb on - strand, within MMJJ_RS06080at 1129.330 kb on - strand, within MMJJ_RS06080at 1129.330 kb on - strand, within MMJJ_RS06080at 1129.331 kb on + strand, within MMJJ_RS06080at 1129.331 kb on + strand, within MMJJ_RS06080at 1129.356 kb on + strand, within MMJJ_RS06080at 1129.382 kb on + strand, within MMJJ_RS06080at 1129.405 kb on + strand, within MMJJ_RS06080at 1129.546 kb on - strand, within MMJJ_RS06080at 1129.547 kb on + strand, within MMJJ_RS06080at 1129.606 kb on - strand, within MMJJ_RS06080at 1129.606 kb on - strand, within MMJJ_RS06080at 1129.607 kb on + strand, within MMJJ_RS06080at 1129.607 kb on + strand, within MMJJ_RS06080at 1129.607 kb on + strand, within MMJJ_RS06080at 1129.607 kb on + strand, within MMJJ_RS06080at 1129.607 kb on + strand, within MMJJ_RS06080at 1129.607 kb on + strand, within MMJJ_RS06080at 1129.607 kb on + strand, within MMJJ_RS06080at 1129.610 kb on - strand, within MMJJ_RS06080at 1129.611 kb on + strand, within MMJJ_RS06080at 1129.611 kb on + strand, within MMJJ_RS06080at 1129.611 kb on + strand, within MMJJ_RS06080at 1129.611 kb on + strand, within MMJJ_RS06080at 1129.644 kb on + strand, within MMJJ_RS06080at 1129.692 kb on - strand, within MMJJ_RS06080at 1129.693 kb on + strand, within MMJJ_RS06080at 1129.714 kb on - strand, within MMJJ_RS06080at 1129.722 kb on + strand, within MMJJ_RS06080at 1129.723 kb on - strand, within MMJJ_RS06080at 1129.779 kb on + strand, within MMJJ_RS06080at 1129.782 kb on + strand, within MMJJ_RS06080at 1129.795 kb on - strand, within MMJJ_RS06080at 1129.795 kb on - strand, within MMJJ_RS06080at 1129.795 kb on - strand, within MMJJ_RS06080at 1129.795 kb on - strand, within MMJJ_RS06080at 1129.798 kb on + strand, within MMJJ_RS06080at 1129.798 kb on + strand, within MMJJ_RS06080at 1129.798 kb on + strand, within MMJJ_RS06080at 1129.798 kb on + strand, within MMJJ_RS06080at 1129.799 kb on - strand, within MMJJ_RS06080at 1129.800 kb on + strand, within MMJJ_RS06080at 1129.800 kb on + strand, within MMJJ_RS06080at 1129.811 kb on - strand, within MMJJ_RS06080at 1129.820 kb on - strand, within MMJJ_RS06080at 1129.820 kb on - strand, within MMJJ_RS06080at 1129.821 kb on + strand, within MMJJ_RS06080at 1129.847 kb on - strandat 1129.847 kb on - strandat 1129.848 kb on + strandat 1129.861 kb on - strandat 1129.862 kb on + strandat 1129.876 kb on + strandat 1129.899 kb on + strandat 1129.899 kb on + strandat 1129.941 kb on + strandat 1129.963 kb on - strandat 1130.071 kb on + strand, within MMJJ_RS06085at 1130.102 kb on + strand, within MMJJ_RS06085at 1130.124 kb on - strand, within MMJJ_RS06085at 1130.125 kb on + strand, within MMJJ_RS06085at 1130.143 kb on + strand, within MMJJ_RS06085at 1130.170 kb on - strand, within MMJJ_RS06085at 1130.214 kb on - strand, within MMJJ_RS06085at 1130.215 kb on + strand, within MMJJ_RS06085at 1130.271 kb on - strand, within MMJJ_RS06085at 1130.315 kb on + strand, within MMJJ_RS06085at 1130.400 kb on + strand, within MMJJ_RS06085at 1130.420 kb on + strand, within MMJJ_RS06085at 1130.515 kb on - strand, within MMJJ_RS06085at 1130.516 kb on + strand, within MMJJ_RS06085at 1130.656 kb on - strandat 1130.657 kb on + strandat 1130.657 kb on + strandat 1130.661 kb on - strandat 1130.662 kb on + strandat 1130.694 kb on + strandat 1130.716 kb on - strandat 1130.794 kb on - strand, within MMJJ_RS06090at 1130.855 kb on - strand, within MMJJ_RS06090at 1130.880 kb on - strand, within MMJJ_RS06090

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride
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1,128,351 + MMJJ_RS06070 0.64 -0.7
1,128,353 - MMJJ_RS06070 0.64 -1.0
1,128,787 - -2.3
1,128,787 - +0.1
1,128,844 + -0.7
1,128,868 - MMJJ_RS06075 0.11 +0.5
1,128,898 - MMJJ_RS06075 0.22 +0.5
1,128,898 - MMJJ_RS06075 0.22 +1.4
1,128,898 - MMJJ_RS06075 0.22 +0.2
1,128,898 - MMJJ_RS06075 0.22 +1.5
1,128,898 - MMJJ_RS06075 0.22 +0.6
1,128,898 - MMJJ_RS06075 0.22 -1.0
1,128,898 - MMJJ_RS06075 0.22 -1.3
1,128,898 - MMJJ_RS06075 0.22 -1.0
1,128,898 - MMJJ_RS06075 0.22 -1.8
1,128,898 - MMJJ_RS06075 0.22 +0.7
1,128,899 + MMJJ_RS06075 0.23 +0.8
1,128,899 + MMJJ_RS06075 0.23 +0.7
1,128,899 + MMJJ_RS06075 0.23 +0.9
1,128,899 + MMJJ_RS06075 0.23 -1.9
1,128,899 + MMJJ_RS06075 0.23 -0.8
1,128,899 + MMJJ_RS06075 0.23 +0.5
1,128,899 + MMJJ_RS06075 0.23 +2.1
1,128,899 + MMJJ_RS06075 0.23 -0.2
1,128,900 - MMJJ_RS06075 0.23 -0.3
1,128,901 + MMJJ_RS06075 0.23 +0.4
1,129,009 - MMJJ_RS06075 0.63 +1.4
1,129,039 + MMJJ_RS06075 0.74 -0.3
1,129,048 + MMJJ_RS06075 0.78 -0.9
1,129,064 - MMJJ_RS06075 0.84 +0.5
1,129,064 - MMJJ_RS06075 0.84 +0.5
1,129,073 + MMJJ_RS06075 0.87 -0.3
1,129,091 - +1.2
1,129,092 + +0.4
1,129,098 - -0.1
1,129,131 - +0.8
1,129,132 + +0.9
1,129,145 - +0.9
1,129,145 - +0.4
1,129,145 - +1.0
1,129,145 - +0.2
1,129,146 + -0.0
1,129,146 + -0.5
1,129,147 - -1.0
1,129,147 - +1.0
1,129,147 - +0.7
1,129,148 + +0.5
1,129,148 + -0.8
1,129,149 - +0.7
1,129,149 - +1.0
1,129,149 - +0.5
1,129,150 + -0.9
1,129,162 + -0.2
1,129,195 + -0.5
1,129,195 + +0.7
1,129,257 - +1.3
1,129,312 - MMJJ_RS06080 0.14 +0.7
1,129,330 - MMJJ_RS06080 0.16 +0.2
1,129,330 - MMJJ_RS06080 0.16 -0.7
1,129,331 + MMJJ_RS06080 0.17 +0.8
1,129,331 + MMJJ_RS06080 0.17 -0.7
1,129,356 + MMJJ_RS06080 0.20 +0.0
1,129,382 + MMJJ_RS06080 0.24 +0.6
1,129,405 + MMJJ_RS06080 0.27 +1.0
1,129,546 - MMJJ_RS06080 0.48 +0.6
1,129,547 + MMJJ_RS06080 0.48 +0.8
1,129,606 - MMJJ_RS06080 0.57 +0.5
1,129,606 - MMJJ_RS06080 0.57 +1.4
1,129,607 + MMJJ_RS06080 0.57 +0.8
1,129,607 + MMJJ_RS06080 0.57 +0.8
1,129,607 + MMJJ_RS06080 0.57 +0.5
1,129,607 + MMJJ_RS06080 0.57 -1.1
1,129,607 + MMJJ_RS06080 0.57 +1.3
1,129,607 + MMJJ_RS06080 0.57 +0.5
1,129,607 + MMJJ_RS06080 0.57 +0.3
1,129,610 - MMJJ_RS06080 0.58 +0.4
1,129,611 + MMJJ_RS06080 0.58 +0.2
1,129,611 + MMJJ_RS06080 0.58 +0.8
1,129,611 + MMJJ_RS06080 0.58 -0.6
1,129,611 + MMJJ_RS06080 0.58 +1.3
1,129,644 + MMJJ_RS06080 0.63 +1.4
1,129,692 - MMJJ_RS06080 0.70 +0.6
1,129,693 + MMJJ_RS06080 0.70 +0.8
1,129,714 - MMJJ_RS06080 0.73 +0.5
1,129,722 + MMJJ_RS06080 0.74 -0.3
1,129,723 - MMJJ_RS06080 0.74 +0.3
1,129,779 + MMJJ_RS06080 0.83 +0.2
1,129,782 + MMJJ_RS06080 0.83 +0.7
1,129,795 - MMJJ_RS06080 0.85 +0.5
1,129,795 - MMJJ_RS06080 0.85 +0.8
1,129,795 - MMJJ_RS06080 0.85 -0.3
1,129,795 - MMJJ_RS06080 0.85 -0.8
1,129,798 + MMJJ_RS06080 0.85 +0.9
1,129,798 + MMJJ_RS06080 0.85 +0.4
1,129,798 + MMJJ_RS06080 0.85 -0.2
1,129,798 + MMJJ_RS06080 0.85 +0.8
1,129,799 - MMJJ_RS06080 0.86 +2.7
1,129,800 + MMJJ_RS06080 0.86 +0.2
1,129,800 + MMJJ_RS06080 0.86 -0.2
1,129,811 - MMJJ_RS06080 0.87 +1.5
1,129,820 - MMJJ_RS06080 0.89 -0.7
1,129,820 - MMJJ_RS06080 0.89 -0.5
1,129,821 + MMJJ_RS06080 0.89 -0.1
1,129,847 - +0.8
1,129,847 - -0.7
1,129,848 + +0.0
1,129,861 - +0.1
1,129,862 + -0.8
1,129,876 + -2.3
1,129,899 + +0.1
1,129,899 + -0.1
1,129,941 + -0.1
1,129,963 - -1.2
1,130,071 + MMJJ_RS06085 0.20 +1.1
1,130,102 + MMJJ_RS06085 0.24 +0.9
1,130,124 - MMJJ_RS06085 0.27 -1.0
1,130,125 + MMJJ_RS06085 0.27 +0.5
1,130,143 + MMJJ_RS06085 0.30 +2.0
1,130,170 - MMJJ_RS06085 0.33 +0.3
1,130,214 - MMJJ_RS06085 0.39 +1.8
1,130,215 + MMJJ_RS06085 0.39 -0.5
1,130,271 - MMJJ_RS06085 0.47 -0.1
1,130,315 + MMJJ_RS06085 0.52 -1.3
1,130,400 + MMJJ_RS06085 0.64 +0.2
1,130,420 + MMJJ_RS06085 0.66 -1.2
1,130,515 - MMJJ_RS06085 0.79 +1.2
1,130,516 + MMJJ_RS06085 0.79 -0.3
1,130,656 - +0.5
1,130,657 + +0.2
1,130,657 + +1.0
1,130,661 - +0.5
1,130,662 + +0.1
1,130,694 + +0.2
1,130,716 - -1.3
1,130,794 - MMJJ_RS06090 0.15 +0.4
1,130,855 - MMJJ_RS06090 0.24 +1.5
1,130,880 - MMJJ_RS06090 0.28 +0.9

Or see this region's nucleotide sequence