Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05650 and MMJJ_RS05655 are separated by 209 nucleotides MMJJ_RS05655 and MMJJ_RS05660 are separated by 1 nucleotides MMJJ_RS05660 and MMJJ_RS05665 are separated by 12 nucleotides
MMJJ_RS05650: MMJJ_RS05650 - formylmethanofuran dehydrogenase subunit B, at 1,054,144 to 1,055,448
_RS05650
MMJJ_RS05655: MMJJ_RS05655 - HisA/HisF family protein, at 1,055,658 to 1,056,326
_RS05655
MMJJ_RS05660: MMJJ_RS05660 - sulfopyruvate decarboxylase subunit beta, at 1,056,328 to 1,056,885
_RS05660
MMJJ_RS05665: MMJJ_RS05665 - hypothetical protein, at 1,056,898 to 1,057,581
_RS05665
Position (kb)
1055
1056
1057 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1054.832 kb on - strand, within MMJJ_RS05650 at 1054.988 kb on + strand at 1054.989 kb on - strand, within MMJJ_RS05650 at 1054.992 kb on - strand, within MMJJ_RS05650 at 1055.023 kb on - strand at 1055.024 kb on + strand, within MMJJ_RS05650 at 1055.139 kb on + strand, within MMJJ_RS05650 at 1055.240 kb on - strand, within MMJJ_RS05650 at 1055.240 kb on - strand, within MMJJ_RS05650 at 1055.241 kb on + strand, within MMJJ_RS05650 at 1055.570 kb on - strand at 1055.570 kb on - strand at 1055.571 kb on + strand at 1055.576 kb on + strand at 1055.598 kb on - strand at 1055.600 kb on - strand at 1055.602 kb on - strand at 1055.603 kb on + strand at 1055.608 kb on + strand at 1055.634 kb on - strand at 1055.639 kb on + strand at 1055.639 kb on + strand at 1055.684 kb on + strand at 1055.684 kb on + strand at 1055.731 kb on + strand, within MMJJ_RS05655 at 1055.750 kb on + strand, within MMJJ_RS05655 at 1055.753 kb on + strand, within MMJJ_RS05655 at 1055.779 kb on - strand, within MMJJ_RS05655 at 1056.058 kb on - strand, within MMJJ_RS05655 at 1056.059 kb on + strand, within MMJJ_RS05655 at 1056.059 kb on + strand, within MMJJ_RS05655 at 1056.059 kb on + strand, within MMJJ_RS05655 at 1056.070 kb on - strand, within MMJJ_RS05655 at 1056.161 kb on - strand, within MMJJ_RS05655 at 1056.161 kb on - strand, within MMJJ_RS05655 at 1056.162 kb on + strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.206 kb on - strand, within MMJJ_RS05655 at 1056.270 kb on + strand at 1056.270 kb on + strand at 1056.275 kb on + strand at 1056.275 kb on + strand at 1056.324 kb on - strand at 1056.324 kb on - strand at 1056.325 kb on + strand at 1056.325 kb on + strand at 1056.328 kb on + strand at 1056.336 kb on - strand at 1056.337 kb on + strand at 1056.386 kb on - strand, within MMJJ_RS05660 at 1056.386 kb on - strand, within MMJJ_RS05660 at 1056.386 kb on - strand, within MMJJ_RS05660 at 1056.426 kb on - strand, within MMJJ_RS05660 at 1056.461 kb on - strand, within MMJJ_RS05660 at 1056.462 kb on + strand, within MMJJ_RS05660 at 1056.462 kb on + strand, within MMJJ_RS05660 at 1056.500 kb on - strand, within MMJJ_RS05660 at 1056.512 kb on - strand, within MMJJ_RS05660 at 1056.524 kb on - strand, within MMJJ_RS05660 at 1056.524 kb on - strand, within MMJJ_RS05660 at 1056.551 kb on - strand, within MMJJ_RS05660 at 1056.552 kb on + strand, within MMJJ_RS05660 at 1056.609 kb on + strand, within MMJJ_RS05660 at 1056.609 kb on + strand, within MMJJ_RS05660 at 1056.609 kb on + strand, within MMJJ_RS05660 at 1056.614 kb on - strand, within MMJJ_RS05660 at 1056.615 kb on + strand, within MMJJ_RS05660 at 1056.615 kb on + strand, within MMJJ_RS05660 at 1056.683 kb on + strand, within MMJJ_RS05660 at 1056.683 kb on + strand, within MMJJ_RS05660 at 1056.683 kb on + strand, within MMJJ_RS05660 at 1056.722 kb on - strand, within MMJJ_RS05660 at 1056.727 kb on + strand, within MMJJ_RS05660 at 1056.761 kb on - strand, within MMJJ_RS05660 at 1056.764 kb on - strand, within MMJJ_RS05660 at 1056.789 kb on - strand, within MMJJ_RS05660 at 1056.790 kb on + strand, within MMJJ_RS05660 at 1056.831 kb on + strand at 1056.831 kb on + strand at 1056.831 kb on + strand at 1056.851 kb on - strand at 1056.886 kb on - strand at 1056.890 kb on - strand at 1056.891 kb on + strand at 1056.969 kb on - strand, within MMJJ_RS05665 at 1057.070 kb on - strand, within MMJJ_RS05665 at 1057.071 kb on + strand, within MMJJ_RS05665 at 1057.092 kb on - strand, within MMJJ_RS05665 at 1057.092 kb on - strand, within MMJJ_RS05665 at 1057.092 kb on - strand, within MMJJ_RS05665 at 1057.093 kb on + strand, within MMJJ_RS05665 at 1057.115 kb on - strand, within MMJJ_RS05665 at 1057.116 kb on + strand, within MMJJ_RS05665 at 1057.116 kb on + strand, within MMJJ_RS05665 at 1057.116 kb on + strand, within MMJJ_RS05665 at 1057.120 kb on - strand, within MMJJ_RS05665 at 1057.121 kb on + strand, within MMJJ_RS05665 at 1057.124 kb on - strand, within MMJJ_RS05665 at 1057.124 kb on - strand, within MMJJ_RS05665 at 1057.125 kb on + strand, within MMJJ_RS05665 at 1057.125 kb on + strand, within MMJJ_RS05665 at 1057.147 kb on + strand, within MMJJ_RS05665 at 1057.172 kb on - strand, within MMJJ_RS05665 at 1057.172 kb on - strand, within MMJJ_RS05665 at 1057.172 kb on - strand, within MMJJ_RS05665 at 1057.173 kb on + strand, within MMJJ_RS05665 at 1057.261 kb on - strand, within MMJJ_RS05665 at 1057.262 kb on + strand, within MMJJ_RS05665 at 1057.262 kb on + strand, within MMJJ_RS05665 at 1057.278 kb on - strand, within MMJJ_RS05665 at 1057.282 kb on - strand, within MMJJ_RS05665 at 1057.282 kb on - strand, within MMJJ_RS05665 at 1057.325 kb on - strand, within MMJJ_RS05665
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,054,832 - MMJJ_RS05650 0.53 -2.0 1,054,988 + -0.5 1,054,989 - MMJJ_RS05650 0.65 -2.7 1,054,992 - MMJJ_RS05650 0.65 -2.5 1,055,023 - -1.2 1,055,024 + MMJJ_RS05650 0.67 -1.9 1,055,139 + MMJJ_RS05650 0.76 -2.5 1,055,240 - MMJJ_RS05650 0.84 -2.8 1,055,240 - MMJJ_RS05650 0.84 -2.0 1,055,241 + MMJJ_RS05650 0.84 -1.7 1,055,570 - -0.7 1,055,570 - -0.8 1,055,571 + +0.1 1,055,576 + +0.2 1,055,598 - -0.2 1,055,600 - +0.6 1,055,602 - +0.1 1,055,603 + +0.5 1,055,608 + -0.1 1,055,634 - +0.1 1,055,639 + +0.3 1,055,639 + -1.2 1,055,684 + -1.7 1,055,684 + -1.7 1,055,731 + MMJJ_RS05655 0.11 -0.9 1,055,750 + MMJJ_RS05655 0.14 -3.3 1,055,753 + MMJJ_RS05655 0.14 -2.2 1,055,779 - MMJJ_RS05655 0.18 -0.7 1,056,058 - MMJJ_RS05655 0.60 -3.3 1,056,059 + MMJJ_RS05655 0.60 -1.7 1,056,059 + MMJJ_RS05655 0.60 -2.2 1,056,059 + MMJJ_RS05655 0.60 -1.2 1,056,070 - MMJJ_RS05655 0.62 -1.0 1,056,161 - MMJJ_RS05655 0.75 -4.0 1,056,161 - MMJJ_RS05655 0.75 -2.2 1,056,162 + MMJJ_RS05655 0.75 -1.5 1,056,189 - MMJJ_RS05655 0.79 -1.6 1,056,189 - MMJJ_RS05655 0.79 -2.1 1,056,189 - MMJJ_RS05655 0.79 -0.6 1,056,189 - MMJJ_RS05655 0.79 -1.7 1,056,189 - MMJJ_RS05655 0.79 -0.4 1,056,190 + MMJJ_RS05655 0.80 -2.8 1,056,190 + MMJJ_RS05655 0.80 -0.9 1,056,190 + MMJJ_RS05655 0.80 -2.7 1,056,190 + MMJJ_RS05655 0.80 -1.0 1,056,206 - MMJJ_RS05655 0.82 -3.1 1,056,270 + -2.7 1,056,270 + -3.6 1,056,275 + -2.2 1,056,275 + +0.2 1,056,324 - +1.2 1,056,324 - -0.5 1,056,325 + -0.9 1,056,325 + +0.0 1,056,328 + +0.4 1,056,336 - +0.6 1,056,337 + -0.1 1,056,386 - MMJJ_RS05660 0.10 +0.4 1,056,386 - MMJJ_RS05660 0.10 -0.5 1,056,386 - MMJJ_RS05660 0.10 -0.0 1,056,426 - MMJJ_RS05660 0.18 -0.4 1,056,461 - MMJJ_RS05660 0.24 -0.2 1,056,462 + MMJJ_RS05660 0.24 -0.8 1,056,462 + MMJJ_RS05660 0.24 +0.2 1,056,500 - MMJJ_RS05660 0.31 -0.9 1,056,512 - MMJJ_RS05660 0.33 +0.2 1,056,524 - MMJJ_RS05660 0.35 +0.2 1,056,524 - MMJJ_RS05660 0.35 -0.6 1,056,551 - MMJJ_RS05660 0.40 +0.1 1,056,552 + MMJJ_RS05660 0.40 -2.0 1,056,609 + MMJJ_RS05660 0.50 -1.5 1,056,609 + MMJJ_RS05660 0.50 +0.4 1,056,609 + MMJJ_RS05660 0.50 -0.4 1,056,614 - MMJJ_RS05660 0.51 -0.1 1,056,615 + MMJJ_RS05660 0.51 -0.2 1,056,615 + MMJJ_RS05660 0.51 -0.3 1,056,683 + MMJJ_RS05660 0.64 +0.1 1,056,683 + MMJJ_RS05660 0.64 +0.5 1,056,683 + MMJJ_RS05660 0.64 -0.5 1,056,722 - MMJJ_RS05660 0.71 -1.6 1,056,727 + MMJJ_RS05660 0.72 -0.9 1,056,761 - MMJJ_RS05660 0.78 +0.3 1,056,764 - MMJJ_RS05660 0.78 +1.0 1,056,789 - MMJJ_RS05660 0.83 -0.5 1,056,790 + MMJJ_RS05660 0.83 -0.2 1,056,831 + +0.4 1,056,831 + -0.5 1,056,831 + -0.3 1,056,851 - -2.8 1,056,886 - +0.0 1,056,890 - +0.6 1,056,891 + +0.1 1,056,969 - MMJJ_RS05665 0.10 +0.5 1,057,070 - MMJJ_RS05665 0.25 -1.0 1,057,071 + MMJJ_RS05665 0.25 -0.0 1,057,092 - MMJJ_RS05665 0.28 +0.3 1,057,092 - MMJJ_RS05665 0.28 -0.5 1,057,092 - MMJJ_RS05665 0.28 +0.9 1,057,093 + MMJJ_RS05665 0.29 +0.1 1,057,115 - MMJJ_RS05665 0.32 +1.1 1,057,116 + MMJJ_RS05665 0.32 -0.1 1,057,116 + MMJJ_RS05665 0.32 +0.1 1,057,116 + MMJJ_RS05665 0.32 +1.2 1,057,120 - MMJJ_RS05665 0.32 -0.8 1,057,121 + MMJJ_RS05665 0.33 +0.4 1,057,124 - MMJJ_RS05665 0.33 -0.4 1,057,124 - MMJJ_RS05665 0.33 +0.4 1,057,125 + MMJJ_RS05665 0.33 +1.2 1,057,125 + MMJJ_RS05665 0.33 -0.3 1,057,147 + MMJJ_RS05665 0.36 +0.7 1,057,172 - MMJJ_RS05665 0.40 -1.7 1,057,172 - MMJJ_RS05665 0.40 -0.2 1,057,172 - MMJJ_RS05665 0.40 -0.0 1,057,173 + MMJJ_RS05665 0.40 -0.1 1,057,261 - MMJJ_RS05665 0.53 -0.2 1,057,262 + MMJJ_RS05665 0.53 -0.1 1,057,262 + MMJJ_RS05665 0.53 +0.4 1,057,278 - MMJJ_RS05665 0.56 -0.4 1,057,282 - MMJJ_RS05665 0.56 +0.2 1,057,282 - MMJJ_RS05665 0.56 +1.2 1,057,325 - MMJJ_RS05665 0.62 +1.0
Or see this region's nucleotide sequence