Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04045 and MMJJ_RS04050 overlap by 8 nucleotides MMJJ_RS04050 and MMJJ_RS04055 are separated by 12 nucleotides MMJJ_RS04055 and MMJJ_RS04060 are separated by 9 nucleotides
MMJJ_RS04045: MMJJ_RS04045 - DHH family phosphoesterase, at 752,036 to 753,052
_RS04045
MMJJ_RS04050: MMJJ_RS04050 - dihydroneopterin aldolase family protein, at 753,045 to 753,419
_RS04050
MMJJ_RS04055: MMJJ_RS04055 - hypothetical protein, at 753,432 to 753,701
_RS04055
MMJJ_RS04060: MMJJ_RS04060 - DUF2341 domain-containing protein, at 753,711 to 755,501
_RS04060
Position (kb)
753
754 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 752.087 kb on + strand at 752.088 kb on - strand at 752.089 kb on + strand at 752.094 kb on - strand at 752.301 kb on - strand, within MMJJ_RS04045 at 752.320 kb on + strand, within MMJJ_RS04045 at 752.350 kb on + strand, within MMJJ_RS04045 at 752.373 kb on - strand, within MMJJ_RS04045 at 752.373 kb on - strand, within MMJJ_RS04045 at 752.398 kb on - strand, within MMJJ_RS04045 at 752.401 kb on + strand, within MMJJ_RS04045 at 752.418 kb on + strand, within MMJJ_RS04045 at 752.420 kb on + strand, within MMJJ_RS04045 at 752.420 kb on + strand, within MMJJ_RS04045 at 752.420 kb on + strand, within MMJJ_RS04045 at 752.458 kb on + strand, within MMJJ_RS04045 at 752.524 kb on - strand, within MMJJ_RS04045 at 752.532 kb on + strand, within MMJJ_RS04045 at 752.573 kb on + strand, within MMJJ_RS04045 at 752.601 kb on - strand, within MMJJ_RS04045 at 752.602 kb on + strand, within MMJJ_RS04045 at 752.617 kb on + strand, within MMJJ_RS04045 at 752.628 kb on - strand, within MMJJ_RS04045 at 752.668 kb on + strand, within MMJJ_RS04045 at 752.692 kb on + strand, within MMJJ_RS04045 at 752.708 kb on - strand, within MMJJ_RS04045 at 752.708 kb on - strand, within MMJJ_RS04045 at 752.709 kb on + strand, within MMJJ_RS04045 at 752.709 kb on + strand, within MMJJ_RS04045 at 752.713 kb on - strand, within MMJJ_RS04045 at 752.721 kb on - strand, within MMJJ_RS04045 at 752.767 kb on + strand, within MMJJ_RS04045 at 752.824 kb on - strand, within MMJJ_RS04045 at 752.829 kb on - strand, within MMJJ_RS04045 at 752.829 kb on - strand, within MMJJ_RS04045 at 752.944 kb on + strand, within MMJJ_RS04045 at 752.971 kb on - strand at 753.031 kb on + strand at 753.101 kb on + strand, within MMJJ_RS04050 at 753.292 kb on + strand, within MMJJ_RS04050 at 753.367 kb on - strand, within MMJJ_RS04050 at 753.403 kb on + strand at 753.530 kb on - strand, within MMJJ_RS04055 at 753.541 kb on - strand, within MMJJ_RS04055 at 753.542 kb on + strand, within MMJJ_RS04055 at 753.544 kb on - strand, within MMJJ_RS04055 at 753.544 kb on - strand, within MMJJ_RS04055 at 753.544 kb on - strand, within MMJJ_RS04055 at 753.584 kb on - strand, within MMJJ_RS04055 at 753.712 kb on - strand at 753.713 kb on + strand at 753.746 kb on + strand at 753.796 kb on - strand at 753.814 kb on - strand at 753.817 kb on - strand at 753.817 kb on - strand at 753.818 kb on + strand at 753.826 kb on - strand at 753.826 kb on - strand at 753.826 kb on - strand at 753.826 kb on - strand at 753.827 kb on + strand at 753.827 kb on + strand at 753.873 kb on - strand at 753.873 kb on - strand at 753.874 kb on + strand at 753.874 kb on + strand at 753.874 kb on + strand at 753.875 kb on - strand at 753.875 kb on - strand at 753.876 kb on + strand at 753.877 kb on - strand at 753.877 kb on - strand at 753.889 kb on + strand at 753.911 kb on - strand, within MMJJ_RS04060 at 753.912 kb on + strand, within MMJJ_RS04060 at 753.937 kb on - strand, within MMJJ_RS04060 at 753.938 kb on + strand, within MMJJ_RS04060 at 753.938 kb on + strand, within MMJJ_RS04060 at 753.945 kb on + strand, within MMJJ_RS04060 at 753.964 kb on - strand, within MMJJ_RS04060 at 753.964 kb on - strand, within MMJJ_RS04060 at 753.964 kb on - strand, within MMJJ_RS04060 at 753.964 kb on - strand, within MMJJ_RS04060 at 753.964 kb on - strand, within MMJJ_RS04060 at 753.964 kb on - strand, within MMJJ_RS04060 at 753.965 kb on + strand, within MMJJ_RS04060 at 753.965 kb on + strand, within MMJJ_RS04060 at 753.965 kb on + strand, within MMJJ_RS04060 at 753.973 kb on - strand, within MMJJ_RS04060 at 753.974 kb on + strand, within MMJJ_RS04060 at 753.974 kb on + strand, within MMJJ_RS04060 at 753.974 kb on + strand, within MMJJ_RS04060 at 753.981 kb on - strand, within MMJJ_RS04060 at 753.982 kb on + strand, within MMJJ_RS04060 at 754.015 kb on - strand, within MMJJ_RS04060 at 754.074 kb on - strand, within MMJJ_RS04060 at 754.075 kb on + strand, within MMJJ_RS04060 at 754.075 kb on + strand, within MMJJ_RS04060 at 754.075 kb on + strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.076 kb on - strand, within MMJJ_RS04060 at 754.077 kb on + strand, within MMJJ_RS04060 at 754.077 kb on + strand, within MMJJ_RS04060 at 754.077 kb on + strand, within MMJJ_RS04060 at 754.077 kb on + strand, within MMJJ_RS04060 at 754.077 kb on + strand, within MMJJ_RS04060 at 754.077 kb on + strand, within MMJJ_RS04060 at 754.077 kb on + strand, within MMJJ_RS04060 at 754.078 kb on - strand, within MMJJ_RS04060 at 754.078 kb on - strand, within MMJJ_RS04060 at 754.078 kb on - strand, within MMJJ_RS04060 at 754.079 kb on + strand, within MMJJ_RS04060 at 754.079 kb on + strand, within MMJJ_RS04060 at 754.079 kb on + strand, within MMJJ_RS04060 at 754.079 kb on + strand, within MMJJ_RS04060 at 754.092 kb on + strand, within MMJJ_RS04060 at 754.092 kb on + strand, within MMJJ_RS04060 at 754.096 kb on - strand, within MMJJ_RS04060 at 754.097 kb on + strand, within MMJJ_RS04060 at 754.119 kb on - strand, within MMJJ_RS04060 at 754.119 kb on - strand, within MMJJ_RS04060 at 754.120 kb on + strand, within MMJJ_RS04060 at 754.120 kb on + strand, within MMJJ_RS04060 at 754.139 kb on - strand, within MMJJ_RS04060 at 754.139 kb on - strand, within MMJJ_RS04060 at 754.139 kb on - strand, within MMJJ_RS04060 at 754.139 kb on - strand, within MMJJ_RS04060 at 754.139 kb on - strand, within MMJJ_RS04060 at 754.140 kb on + strand, within MMJJ_RS04060 at 754.140 kb on + strand, within MMJJ_RS04060 at 754.140 kb on + strand, within MMJJ_RS04060 at 754.140 kb on + strand, within MMJJ_RS04060 at 754.141 kb on - strand, within MMJJ_RS04060 at 754.141 kb on - strand, within MMJJ_RS04060 at 754.141 kb on - strand, within MMJJ_RS04060 at 754.141 kb on - strand, within MMJJ_RS04060 at 754.142 kb on + strand, within MMJJ_RS04060 at 754.186 kb on - strand, within MMJJ_RS04060 at 754.186 kb on - strand, within MMJJ_RS04060 at 754.187 kb on + strand, within MMJJ_RS04060 at 754.187 kb on + strand, within MMJJ_RS04060 at 754.187 kb on + strand, within MMJJ_RS04060 at 754.187 kb on + strand, within MMJJ_RS04060 at 754.187 kb on + strand, within MMJJ_RS04060 at 754.197 kb on - strand, within MMJJ_RS04060 at 754.198 kb on + strand, within MMJJ_RS04060 at 754.203 kb on + strand, within MMJJ_RS04060 at 754.205 kb on + strand, within MMJJ_RS04060 at 754.205 kb on - strand, within MMJJ_RS04060 at 754.205 kb on - strand, within MMJJ_RS04060 at 754.205 kb on - strand, within MMJJ_RS04060 at 754.205 kb on - strand, within MMJJ_RS04060 at 754.206 kb on + strand, within MMJJ_RS04060 at 754.207 kb on - strand, within MMJJ_RS04060 at 754.269 kb on - strand, within MMJJ_RS04060 at 754.270 kb on + strand, within MMJJ_RS04060 at 754.270 kb on + strand, within MMJJ_RS04060 at 754.290 kb on + strand, within MMJJ_RS04060 at 754.293 kb on - strand, within MMJJ_RS04060 at 754.293 kb on - strand, within MMJJ_RS04060 at 754.294 kb on + strand, within MMJJ_RS04060 at 754.294 kb on + strand, within MMJJ_RS04060 at 754.294 kb on + strand, within MMJJ_RS04060 at 754.305 kb on - strand, within MMJJ_RS04060 at 754.327 kb on - strand, within MMJJ_RS04060 at 754.328 kb on + strand, within MMJJ_RS04060 at 754.350 kb on + strand, within MMJJ_RS04060 at 754.350 kb on + strand, within MMJJ_RS04060 at 754.363 kb on - strand, within MMJJ_RS04060 at 754.364 kb on + strand, within MMJJ_RS04060 at 754.364 kb on + strand, within MMJJ_RS04060 at 754.364 kb on + strand, within MMJJ_RS04060 at 754.394 kb on - strand, within MMJJ_RS04060 at 754.394 kb on - strand, within MMJJ_RS04060 at 754.394 kb on - strand, within MMJJ_RS04060 at 754.394 kb on - strand, within MMJJ_RS04060 at 754.394 kb on - strand, within MMJJ_RS04060 at 754.394 kb on - strand at 754.394 kb on - strand, within MMJJ_RS04060 at 754.394 kb on - strand, within MMJJ_RS04060 at 754.395 kb on + strand, within MMJJ_RS04060 at 754.395 kb on + strand, within MMJJ_RS04060 at 754.395 kb on + strand, within MMJJ_RS04060 at 754.395 kb on + strand, within MMJJ_RS04060 at 754.395 kb on + strand, within MMJJ_RS04060 at 754.395 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 752,087 + -0.6 752,088 - -1.7 752,089 + -1.4 752,094 - -1.3 752,301 - MMJJ_RS04045 0.26 -0.0 752,320 + MMJJ_RS04045 0.28 -0.3 752,350 + MMJJ_RS04045 0.31 -2.1 752,373 - MMJJ_RS04045 0.33 -0.5 752,373 - MMJJ_RS04045 0.33 -0.3 752,398 - MMJJ_RS04045 0.36 -0.6 752,401 + MMJJ_RS04045 0.36 -0.6 752,418 + MMJJ_RS04045 0.38 -1.6 752,420 + MMJJ_RS04045 0.38 -0.3 752,420 + MMJJ_RS04045 0.38 -1.1 752,420 + MMJJ_RS04045 0.38 -2.3 752,458 + MMJJ_RS04045 0.41 -0.0 752,524 - MMJJ_RS04045 0.48 -0.7 752,532 + MMJJ_RS04045 0.49 -0.7 752,573 + MMJJ_RS04045 0.53 -0.9 752,601 - MMJJ_RS04045 0.56 -1.5 752,602 + MMJJ_RS04045 0.56 +0.0 752,617 + MMJJ_RS04045 0.57 -0.4 752,628 - MMJJ_RS04045 0.58 -1.4 752,668 + MMJJ_RS04045 0.62 -0.3 752,692 + MMJJ_RS04045 0.65 -1.0 752,708 - MMJJ_RS04045 0.66 +0.7 752,708 - MMJJ_RS04045 0.66 +0.2 752,709 + MMJJ_RS04045 0.66 +0.6 752,709 + MMJJ_RS04045 0.66 -1.6 752,713 - MMJJ_RS04045 0.67 -0.9 752,721 - MMJJ_RS04045 0.67 -0.9 752,767 + MMJJ_RS04045 0.72 -0.4 752,824 - MMJJ_RS04045 0.77 -0.8 752,829 - MMJJ_RS04045 0.78 -0.7 752,829 - MMJJ_RS04045 0.78 -0.9 752,944 + MMJJ_RS04045 0.89 -0.2 752,971 - -1.6 753,031 + +0.3 753,101 + MMJJ_RS04050 0.15 -3.0 753,292 + MMJJ_RS04050 0.66 -2.6 753,367 - MMJJ_RS04050 0.86 -2.6 753,403 + -2.6 753,530 - MMJJ_RS04055 0.36 +1.2 753,541 - MMJJ_RS04055 0.40 +0.0 753,542 + MMJJ_RS04055 0.41 -0.3 753,544 - MMJJ_RS04055 0.41 +0.1 753,544 - MMJJ_RS04055 0.41 +1.0 753,544 - MMJJ_RS04055 0.41 +0.1 753,584 - MMJJ_RS04055 0.56 -0.1 753,712 - -0.5 753,713 + -0.7 753,746 + +0.5 753,796 - +0.4 753,814 - +0.1 753,817 - -0.4 753,817 - -0.3 753,818 + +0.6 753,826 - -1.2 753,826 - +1.2 753,826 - +0.3 753,826 - +0.0 753,827 + -0.6 753,827 + +0.6 753,873 - -1.3 753,873 - +0.0 753,874 + -0.1 753,874 + -0.6 753,874 + -1.0 753,875 - -1.5 753,875 - -0.9 753,876 + -1.2 753,877 - -0.3 753,877 - +0.9 753,889 + +0.1 753,911 - MMJJ_RS04060 0.11 +0.3 753,912 + MMJJ_RS04060 0.11 +1.1 753,937 - MMJJ_RS04060 0.13 +0.1 753,938 + MMJJ_RS04060 0.13 -1.0 753,938 + MMJJ_RS04060 0.13 +0.2 753,945 + MMJJ_RS04060 0.13 -1.4 753,964 - MMJJ_RS04060 0.14 -0.1 753,964 - MMJJ_RS04060 0.14 -0.3 753,964 - MMJJ_RS04060 0.14 -1.0 753,964 - MMJJ_RS04060 0.14 +0.8 753,964 - MMJJ_RS04060 0.14 -1.1 753,964 - MMJJ_RS04060 0.14 -0.1 753,965 + MMJJ_RS04060 0.14 -0.3 753,965 + MMJJ_RS04060 0.14 +0.5 753,965 + MMJJ_RS04060 0.14 +0.1 753,973 - MMJJ_RS04060 0.15 -0.4 753,974 + MMJJ_RS04060 0.15 -0.2 753,974 + MMJJ_RS04060 0.15 -0.7 753,974 + MMJJ_RS04060 0.15 +0.3 753,981 - MMJJ_RS04060 0.15 +0.4 753,982 + MMJJ_RS04060 0.15 -0.5 754,015 - MMJJ_RS04060 0.17 +0.3 754,074 - MMJJ_RS04060 0.20 -0.4 754,075 + MMJJ_RS04060 0.20 +0.1 754,075 + MMJJ_RS04060 0.20 +0.6 754,075 + MMJJ_RS04060 0.20 +0.2 754,076 - MMJJ_RS04060 0.20 -1.1 754,076 - MMJJ_RS04060 0.20 +0.5 754,076 - MMJJ_RS04060 0.20 +0.5 754,076 - MMJJ_RS04060 0.20 -0.2 754,076 - MMJJ_RS04060 0.20 -2.7 754,076 - MMJJ_RS04060 0.20 +0.1 754,076 - MMJJ_RS04060 0.20 -0.8 754,076 - MMJJ_RS04060 0.20 +0.5 754,076 - MMJJ_RS04060 0.20 +0.3 754,076 - MMJJ_RS04060 0.20 -1.1 754,076 - MMJJ_RS04060 0.20 -0.1 754,077 + MMJJ_RS04060 0.20 -0.0 754,077 + MMJJ_RS04060 0.20 -0.3 754,077 + MMJJ_RS04060 0.20 -0.2 754,077 + MMJJ_RS04060 0.20 -0.3 754,077 + MMJJ_RS04060 0.20 -0.1 754,077 + MMJJ_RS04060 0.20 -0.6 754,077 + MMJJ_RS04060 0.20 +0.9 754,078 - MMJJ_RS04060 0.20 +0.3 754,078 - MMJJ_RS04060 0.20 +0.7 754,078 - MMJJ_RS04060 0.20 +0.5 754,079 + MMJJ_RS04060 0.21 +1.2 754,079 + MMJJ_RS04060 0.21 -0.4 754,079 + MMJJ_RS04060 0.21 -0.2 754,079 + MMJJ_RS04060 0.21 +1.4 754,092 + MMJJ_RS04060 0.21 +0.8 754,092 + MMJJ_RS04060 0.21 +0.6 754,096 - MMJJ_RS04060 0.21 +0.7 754,097 + MMJJ_RS04060 0.22 -0.5 754,119 - MMJJ_RS04060 0.23 +0.0 754,119 - MMJJ_RS04060 0.23 +0.7 754,120 + MMJJ_RS04060 0.23 -0.9 754,120 + MMJJ_RS04060 0.23 +0.4 754,139 - MMJJ_RS04060 0.24 +0.6 754,139 - MMJJ_RS04060 0.24 +0.4 754,139 - MMJJ_RS04060 0.24 -1.1 754,139 - MMJJ_RS04060 0.24 +0.1 754,139 - MMJJ_RS04060 0.24 +0.0 754,140 + MMJJ_RS04060 0.24 +0.5 754,140 + MMJJ_RS04060 0.24 +0.5 754,140 + MMJJ_RS04060 0.24 -0.8 754,140 + MMJJ_RS04060 0.24 -0.1 754,141 - MMJJ_RS04060 0.24 +1.0 754,141 - MMJJ_RS04060 0.24 -0.6 754,141 - MMJJ_RS04060 0.24 -1.3 754,141 - MMJJ_RS04060 0.24 +0.7 754,142 + MMJJ_RS04060 0.24 -0.8 754,186 - MMJJ_RS04060 0.27 -0.2 754,186 - MMJJ_RS04060 0.27 +0.3 754,187 + MMJJ_RS04060 0.27 -0.6 754,187 + MMJJ_RS04060 0.27 -0.2 754,187 + MMJJ_RS04060 0.27 +0.1 754,187 + MMJJ_RS04060 0.27 +0.9 754,187 + MMJJ_RS04060 0.27 +0.5 754,197 - MMJJ_RS04060 0.27 +0.8 754,198 + MMJJ_RS04060 0.27 +0.1 754,203 + MMJJ_RS04060 0.27 +0.2 754,205 + MMJJ_RS04060 0.28 -1.0 754,205 - MMJJ_RS04060 0.28 -1.2 754,205 - MMJJ_RS04060 0.28 -0.2 754,205 - MMJJ_RS04060 0.28 -1.1 754,205 - MMJJ_RS04060 0.28 +0.1 754,206 + MMJJ_RS04060 0.28 +0.8 754,207 - MMJJ_RS04060 0.28 -1.6 754,269 - MMJJ_RS04060 0.31 -0.3 754,270 + MMJJ_RS04060 0.31 +0.5 754,270 + MMJJ_RS04060 0.31 +0.3 754,290 + MMJJ_RS04060 0.32 +0.1 754,293 - MMJJ_RS04060 0.32 -1.3 754,293 - MMJJ_RS04060 0.32 +1.0 754,294 + MMJJ_RS04060 0.33 -0.2 754,294 + MMJJ_RS04060 0.33 -1.9 754,294 + MMJJ_RS04060 0.33 +0.7 754,305 - MMJJ_RS04060 0.33 -0.6 754,327 - MMJJ_RS04060 0.34 +0.8 754,328 + MMJJ_RS04060 0.34 -0.4 754,350 + MMJJ_RS04060 0.36 +0.6 754,350 + MMJJ_RS04060 0.36 +0.2 754,363 - MMJJ_RS04060 0.36 +0.1 754,364 + MMJJ_RS04060 0.36 +0.2 754,364 + MMJJ_RS04060 0.36 +0.2 754,364 + MMJJ_RS04060 0.36 -0.9 754,394 - MMJJ_RS04060 0.38 -0.3 754,394 - MMJJ_RS04060 0.38 -0.7 754,394 - MMJJ_RS04060 0.38 +0.3 754,394 - MMJJ_RS04060 0.38 -2.4 754,394 - MMJJ_RS04060 0.38 +0.2 754,394 - -1.0 754,394 - MMJJ_RS04060 0.38 -0.3 754,394 - MMJJ_RS04060 0.38 -0.6 754,395 + MMJJ_RS04060 0.38 +1.5 754,395 + MMJJ_RS04060 0.38 +0.3 754,395 + MMJJ_RS04060 0.38 -0.2 754,395 + MMJJ_RS04060 0.38 +0.6 754,395 + MMJJ_RS04060 0.38 -0.7 754,395 + -1.0
Or see this region's nucleotide sequence