Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03640 and MMJJ_RS03645 are separated by 66 nucleotides MMJJ_RS03645 and MMJJ_RS03650 are separated by 68 nucleotides MMJJ_RS03650 and MMJJ_RS03655 are separated by 9 nucleotides
MMJJ_RS03640: MMJJ_RS03640 - type II glyceraldehyde-3-phosphate dehydrogenase, at 679,447 to 680,469
_RS03640
MMJJ_RS03645: MMJJ_RS03645 - hypothetical protein, at 680,536 to 680,916
_RS03645
MMJJ_RS03650: MMJJ_RS03650 - class I SAM-dependent methyltransferase family protein, at 680,985 to 682,013
_RS03650
MMJJ_RS03655: MMJJ_RS03655 - replication factor C large subunit, at 682,023 to 683,483
_RS03655
Position (kb) 680
681
682
683 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 680.391 kb on - strand at 680.459 kb on - strand at 680.491 kb on + strand at 680.494 kb on - strand at 680.508 kb on - strand at 680.524 kb on + strand at 680.542 kb on - strand at 680.547 kb on + strand at 680.612 kb on - strand at 680.617 kb on + strand, within MMJJ_RS03645 at 680.619 kb on + strand at 680.645 kb on - strand, within MMJJ_RS03645 at 680.937 kb on - strand at 680.937 kb on - strand at 680.937 kb on - strand at 680.937 kb on - strand at 680.937 kb on - strand at 680.938 kb on + strand at 680.938 kb on + strand at 680.938 kb on + strand at 680.938 kb on + strand at 680.939 kb on - strand at 680.939 kb on - strand at 680.940 kb on + strand at 680.948 kb on - strand at 680.948 kb on - strand at 680.954 kb on - strand at 680.954 kb on - strand at 680.955 kb on + strand at 680.955 kb on + strand at 680.955 kb on + strand at 680.958 kb on - strand at 680.983 kb on - strand at 681.055 kb on - strand at 681.056 kb on + strand at 681.059 kb on - strand at 681.062 kb on - strand at 681.099 kb on - strand, within MMJJ_RS03650 at 681.286 kb on + strand, within MMJJ_RS03650 at 681.476 kb on - strand, within MMJJ_RS03650 at 681.477 kb on + strand, within MMJJ_RS03650 at 681.483 kb on - strand, within MMJJ_RS03650 at 681.483 kb on - strand, within MMJJ_RS03650 at 681.484 kb on + strand, within MMJJ_RS03650 at 681.484 kb on + strand, within MMJJ_RS03650 at 681.528 kb on - strand, within MMJJ_RS03650 at 681.577 kb on - strand, within MMJJ_RS03650 at 681.578 kb on + strand, within MMJJ_RS03650 at 681.595 kb on - strand, within MMJJ_RS03650 at 681.595 kb on - strand, within MMJJ_RS03650 at 681.596 kb on + strand, within MMJJ_RS03650 at 681.629 kb on - strand, within MMJJ_RS03650 at 681.630 kb on + strand, within MMJJ_RS03650 at 681.712 kb on - strand, within MMJJ_RS03650 at 681.713 kb on + strand, within MMJJ_RS03650 at 681.788 kb on + strand, within MMJJ_RS03650 at 681.865 kb on + strand, within MMJJ_RS03650 at 681.868 kb on + strand, within MMJJ_RS03650 at 681.958 kb on + strand at 681.976 kb on - strand at 681.976 kb on - strand at 681.976 kb on - strand at 681.977 kb on + strand at 681.977 kb on + strand at 681.983 kb on + strand at 681.997 kb on - strand at 681.998 kb on + strand at 682.001 kb on + strand at 682.012 kb on + strand at 682.017 kb on + strand at 682.161 kb on + strand at 682.186 kb on + strand, within MMJJ_RS03655 at 682.186 kb on + strand, within MMJJ_RS03655 at 682.242 kb on + strand, within MMJJ_RS03655 at 682.260 kb on - strand, within MMJJ_RS03655 at 682.311 kb on + strand, within MMJJ_RS03655 at 682.512 kb on + strand, within MMJJ_RS03655 at 682.732 kb on + strand, within MMJJ_RS03655
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 680,391 - +1.0 680,459 - -1.4 680,491 + +0.8 680,494 - -1.3 680,508 - +0.5 680,524 + -1.2 680,542 - -1.8 680,547 + -1.6 680,612 - -1.3 680,617 + MMJJ_RS03645 0.21 -2.0 680,619 + -1.8 680,645 - MMJJ_RS03645 0.29 -2.1 680,937 - +0.1 680,937 - -0.5 680,937 - +0.6 680,937 - -1.2 680,937 - +0.9 680,938 + +0.6 680,938 + +0.9 680,938 + +1.1 680,938 + +0.5 680,939 - -0.8 680,939 - -0.2 680,940 + -0.8 680,948 - -1.4 680,948 - -0.7 680,954 - -0.2 680,954 - -2.3 680,955 + +0.2 680,955 + -0.9 680,955 + +0.7 680,958 - +0.5 680,983 - -2.7 681,055 - -3.2 681,056 + -1.0 681,059 - -1.3 681,062 - -1.3 681,099 - MMJJ_RS03650 0.11 -1.9 681,286 + MMJJ_RS03650 0.29 -0.2 681,476 - MMJJ_RS03650 0.48 -2.1 681,477 + MMJJ_RS03650 0.48 -1.2 681,483 - MMJJ_RS03650 0.48 -0.6 681,483 - MMJJ_RS03650 0.48 -3.4 681,484 + MMJJ_RS03650 0.48 -2.1 681,484 + MMJJ_RS03650 0.48 -3.0 681,528 - MMJJ_RS03650 0.53 -3.8 681,577 - MMJJ_RS03650 0.58 -3.1 681,578 + MMJJ_RS03650 0.58 -2.9 681,595 - MMJJ_RS03650 0.59 -3.4 681,595 - MMJJ_RS03650 0.59 -1.0 681,596 + MMJJ_RS03650 0.59 +0.0 681,629 - MMJJ_RS03650 0.63 -2.8 681,630 + MMJJ_RS03650 0.63 -1.7 681,712 - MMJJ_RS03650 0.71 -1.8 681,713 + MMJJ_RS03650 0.71 -1.9 681,788 + MMJJ_RS03650 0.78 -3.0 681,865 + MMJJ_RS03650 0.86 -2.1 681,868 + MMJJ_RS03650 0.86 -3.0 681,958 + -2.0 681,976 - -3.1 681,976 - -1.0 681,976 - -2.1 681,977 + -0.6 681,977 + -0.8 681,983 + -1.8 681,997 - +0.3 681,998 + -0.0 682,001 + +0.1 682,012 + -0.5 682,017 + +0.6 682,161 + +0.1 682,186 + MMJJ_RS03655 0.11 -1.0 682,186 + MMJJ_RS03655 0.11 -0.2 682,242 + MMJJ_RS03655 0.15 -0.3 682,260 - MMJJ_RS03655 0.16 -0.4 682,311 + MMJJ_RS03655 0.20 -1.8 682,512 + MMJJ_RS03655 0.33 -1.1 682,732 + MMJJ_RS03655 0.49 -3.7
Or see this region's nucleotide sequence