Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03185 and MMJJ_RS03190 are separated by 199 nucleotides MMJJ_RS03190 and MMJJ_RS03195 are separated by 241 nucleotides MMJJ_RS03195 and MMJJ_RS03200 are separated by 15 nucleotides
MMJJ_RS03185: MMJJ_RS03185 - alanine--tRNA ligase, at 601,763 to 604,441
_RS03185
MMJJ_RS03190: MMJJ_RS03190 - phosphopyruvate hydratase, at 604,641 to 605,933
_RS03190
MMJJ_RS03195: MMJJ_RS03195 - hypothetical protein, at 606,175 to 606,453
_RS03195
MMJJ_RS03200: MMJJ_RS03200 - uroporphyrinogen-III synthase, at 606,469 to 607,203
_RS03200
Position (kb) 604
605
606 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 604.330 kb on - strand at 604.331 kb on + strand at 604.375 kb on - strand at 604.497 kb on + strand at 604.541 kb on - strand at 604.542 kb on + strand at 604.542 kb on + strand at 604.546 kb on - strand at 604.547 kb on + strand at 604.548 kb on - strand at 604.562 kb on + strand at 604.586 kb on - strand at 604.595 kb on - strand at 604.600 kb on + strand at 604.626 kb on - strand at 604.632 kb on - strand at 604.632 kb on - strand at 604.633 kb on + strand at 604.643 kb on + strand at 604.643 kb on + strand at 605.171 kb on + strand, within MMJJ_RS03190 at 605.304 kb on - strand at 605.310 kb on - strand at 605.311 kb on + strand, within MMJJ_RS03190 at 605.340 kb on - strand, within MMJJ_RS03190 at 605.360 kb on + strand, within MMJJ_RS03190 at 605.498 kb on + strand, within MMJJ_RS03190 at 605.518 kb on - strand, within MMJJ_RS03190 at 605.581 kb on + strand, within MMJJ_RS03190 at 605.679 kb on - strand, within MMJJ_RS03190 at 605.971 kb on - strand at 605.998 kb on + strand at 606.027 kb on - strand at 606.043 kb on + strand at 606.099 kb on + strand at 606.106 kb on + strand at 606.118 kb on - strand at 606.122 kb on - strand at 606.122 kb on - strand at 606.123 kb on + strand at 606.123 kb on + strand at 606.124 kb on - strand at 606.142 kb on - strand at 606.155 kb on + strand at 606.155 kb on + strand at 606.172 kb on + strand at 606.195 kb on + strand at 606.195 kb on + strand at 606.198 kb on - strand at 606.216 kb on - strand, within MMJJ_RS03195 at 606.276 kb on + strand, within MMJJ_RS03195 at 606.346 kb on - strand, within MMJJ_RS03195 at 606.347 kb on + strand, within MMJJ_RS03195 at 606.414 kb on + strand, within MMJJ_RS03195 at 606.422 kb on + strand, within MMJJ_RS03195 at 606.422 kb on + strand, within MMJJ_RS03195 at 606.422 kb on + strand, within MMJJ_RS03195 at 606.618 kb on + strand, within MMJJ_RS03200 at 606.775 kb on - strand, within MMJJ_RS03200 at 606.775 kb on - strand at 606.859 kb on + strand, within MMJJ_RS03200
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 604,330 - -1.8 604,331 + -1.0 604,375 - -1.3 604,497 + -2.0 604,541 - +0.8 604,542 + +0.8 604,542 + +0.0 604,546 - +1.1 604,547 + -0.1 604,548 - +0.0 604,562 + -0.3 604,586 - +0.8 604,595 - +0.7 604,600 + +0.7 604,626 - +0.7 604,632 - +0.6 604,632 - -0.2 604,633 + +0.7 604,643 + +0.7 604,643 + -0.9 605,171 + MMJJ_RS03190 0.41 -2.6 605,304 - +1.0 605,310 - -1.3 605,311 + MMJJ_RS03190 0.52 -2.6 605,340 - MMJJ_RS03190 0.54 -2.4 605,360 + MMJJ_RS03190 0.56 -1.3 605,498 + MMJJ_RS03190 0.66 +0.0 605,518 - MMJJ_RS03190 0.68 -1.6 605,581 + MMJJ_RS03190 0.73 -2.4 605,679 - MMJJ_RS03190 0.80 -0.3 605,971 - +0.0 605,998 + -2.7 606,027 - +0.1 606,043 + -3.4 606,099 + -0.1 606,106 + +0.1 606,118 - -1.4 606,122 - -1.1 606,122 - -0.1 606,123 + +1.3 606,123 + -1.1 606,124 - +0.2 606,142 - -1.2 606,155 + +1.6 606,155 + -1.8 606,172 + +0.0 606,195 + +0.0 606,195 + +0.9 606,198 - -2.6 606,216 - MMJJ_RS03195 0.15 -1.5 606,276 + MMJJ_RS03195 0.36 -0.2 606,346 - MMJJ_RS03195 0.61 -0.9 606,347 + MMJJ_RS03195 0.62 +1.1 606,414 + MMJJ_RS03195 0.86 +0.7 606,422 + MMJJ_RS03195 0.89 -0.1 606,422 + MMJJ_RS03195 0.89 +0.2 606,422 + MMJJ_RS03195 0.89 -0.3 606,618 + MMJJ_RS03200 0.20 -3.0 606,775 - MMJJ_RS03200 0.42 -1.3 606,775 - +0.0 606,859 + MMJJ_RS03200 0.53 -1.3
Or see this region's nucleotide sequence