Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03085 and MMJJ_RS03090 are separated by 207 nucleotides MMJJ_RS03090 and MMJJ_RS03095 are separated by 87 nucleotides MMJJ_RS03095 and MMJJ_RS03100 are separated by 172 nucleotides
MMJJ_RS03085: MMJJ_RS03085 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 579,633 to 580,349
_RS03085
MMJJ_RS03090: MMJJ_RS03090 - ECF transporter S component, at 580,557 to 581,153
_RS03090
MMJJ_RS03095: MMJJ_RS03095 - bifunctional N(6)-L-threonylcarbamoyladenine synthase/serine/threonine protein kinase, at 581,241 to 582,884
_RS03095
MMJJ_RS03100: MMJJ_RS03100 - threonine--tRNA ligase, at 583,057 to 584,925
_RS03100
Position (kb) 581
582
583 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 580.402 kb on - strand at 580.434 kb on - strand at 580.435 kb on + strand at 580.436 kb on - strand at 580.436 kb on - strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.442 kb on - strand at 580.442 kb on - strand at 580.442 kb on - strand at 580.446 kb on - strand at 580.482 kb on + strand at 580.489 kb on - strand at 580.510 kb on + strand at 580.541 kb on - strand at 580.559 kb on + strand at 580.588 kb on + strand at 580.588 kb on + strand at 580.588 kb on + strand at 580.635 kb on - strand, within MMJJ_RS03090 at 580.636 kb on + strand, within MMJJ_RS03090 at 580.672 kb on + strand, within MMJJ_RS03090 at 580.672 kb on + strand, within MMJJ_RS03090 at 580.692 kb on - strand at 580.693 kb on + strand, within MMJJ_RS03090 at 580.693 kb on + strand, within MMJJ_RS03090 at 580.693 kb on + strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.730 kb on + strand, within MMJJ_RS03090 at 580.730 kb on + strand, within MMJJ_RS03090 at 580.730 kb on + strand, within MMJJ_RS03090 at 580.730 kb on + strand, within MMJJ_RS03090 at 580.730 kb on + strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.732 kb on + strand, within MMJJ_RS03090 at 580.732 kb on + strand, within MMJJ_RS03090 at 580.737 kb on - strand, within MMJJ_RS03090 at 580.737 kb on - strand, within MMJJ_RS03090 at 580.738 kb on + strand, within MMJJ_RS03090 at 580.738 kb on + strand, within MMJJ_RS03090 at 580.738 kb on + strand, within MMJJ_RS03090 at 580.738 kb on + strand, within MMJJ_RS03090 at 580.761 kb on + strand, within MMJJ_RS03090 at 580.777 kb on - strand, within MMJJ_RS03090 at 580.777 kb on - strand, within MMJJ_RS03090 at 580.778 kb on + strand, within MMJJ_RS03090 at 580.781 kb on + strand, within MMJJ_RS03090 at 580.781 kb on + strand, within MMJJ_RS03090 at 580.789 kb on + strand, within MMJJ_RS03090 at 580.789 kb on + strand, within MMJJ_RS03090 at 580.789 kb on + strand, within MMJJ_RS03090 at 580.789 kb on + strand, within MMJJ_RS03090 at 580.790 kb on - strand, within MMJJ_RS03090 at 580.790 kb on - strand, within MMJJ_RS03090 at 580.791 kb on + strand, within MMJJ_RS03090 at 580.791 kb on + strand, within MMJJ_RS03090 at 580.834 kb on + strand, within MMJJ_RS03090 at 580.834 kb on + strand, within MMJJ_RS03090 at 580.835 kb on - strand, within MMJJ_RS03090 at 580.843 kb on - strand, within MMJJ_RS03090 at 580.844 kb on + strand, within MMJJ_RS03090 at 580.874 kb on - strand, within MMJJ_RS03090 at 580.908 kb on - strand, within MMJJ_RS03090 at 580.909 kb on + strand, within MMJJ_RS03090 at 580.918 kb on - strand, within MMJJ_RS03090 at 580.918 kb on - strand, within MMJJ_RS03090 at 580.919 kb on + strand, within MMJJ_RS03090 at 580.922 kb on + strand, within MMJJ_RS03090 at 580.922 kb on + strand, within MMJJ_RS03090 at 580.933 kb on - strand, within MMJJ_RS03090 at 580.933 kb on - strand, within MMJJ_RS03090 at 581.015 kb on - strand, within MMJJ_RS03090 at 581.015 kb on - strand, within MMJJ_RS03090 at 581.016 kb on + strand, within MMJJ_RS03090 at 581.016 kb on + strand, within MMJJ_RS03090 at 581.028 kb on + strand, within MMJJ_RS03090 at 581.028 kb on + strand, within MMJJ_RS03090 at 581.031 kb on - strand, within MMJJ_RS03090 at 581.053 kb on - strand, within MMJJ_RS03090 at 581.053 kb on - strand, within MMJJ_RS03090 at 581.054 kb on + strand, within MMJJ_RS03090 at 581.054 kb on + strand, within MMJJ_RS03090 at 581.054 kb on + strand, within MMJJ_RS03090 at 581.058 kb on + strand, within MMJJ_RS03090 at 581.074 kb on + strand, within MMJJ_RS03090 at 581.074 kb on + strand, within MMJJ_RS03090 at 581.107 kb on - strand at 581.142 kb on - strand at 581.153 kb on - strand at 581.153 kb on - strand at 581.154 kb on + strand at 581.156 kb on - strand at 581.156 kb on - strand at 581.157 kb on + strand at 581.162 kb on + strand at 581.162 kb on + strand at 581.172 kb on - strand at 581.173 kb on + strand at 581.184 kb on + strand at 581.197 kb on - strand at 581.197 kb on - strand at 581.197 kb on - strand at 581.198 kb on + strand at 581.198 kb on + strand at 581.198 kb on + strand at 581.201 kb on - strand at 581.201 kb on - strand at 581.201 kb on - strand at 581.202 kb on + strand at 581.202 kb on + strand at 581.202 kb on + strand at 581.202 kb on + strand at 581.203 kb on - strand at 581.206 kb on + strand at 581.230 kb on + strand at 581.248 kb on - strand at 581.249 kb on + strand at 581.249 kb on + strand at 581.249 kb on + strand at 581.249 kb on + strand at 581.249 kb on + strand at 581.306 kb on + strand at 581.330 kb on - strand at 581.415 kb on - strand, within MMJJ_RS03095 at 582.140 kb on - strand, within MMJJ_RS03095 at 582.143 kb on - strand, within MMJJ_RS03095 at 582.335 kb on + strand, within MMJJ_RS03095 at 582.484 kb on + strand, within MMJJ_RS03095 at 582.487 kb on - strand, within MMJJ_RS03095 at 582.946 kb on + strand at 582.964 kb on - strand at 583.003 kb on + strand at 583.018 kb on - strand at 583.041 kb on - strand at 583.520 kb on - strand, within MMJJ_RS03100
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 580,402 - -1.8 580,434 - -1.3 580,435 + -1.1 580,436 - +0.1 580,436 - +0.6 580,437 + -1.5 580,437 + -3.2 580,437 + -0.7 580,437 + +0.9 580,437 + -2.0 580,437 + -2.3 580,437 + -0.2 580,442 - -1.2 580,442 - -0.6 580,442 - +0.6 580,446 - +0.9 580,482 + +1.1 580,489 - +0.1 580,510 + +0.0 580,541 - -1.9 580,559 + +0.4 580,588 + -0.5 580,588 + +1.0 580,588 + +0.1 580,635 - MMJJ_RS03090 0.13 +0.0 580,636 + MMJJ_RS03090 0.13 +0.3 580,672 + MMJJ_RS03090 0.19 +0.1 580,672 + MMJJ_RS03090 0.19 +0.1 580,692 - -0.6 580,693 + MMJJ_RS03090 0.23 -0.4 580,693 + MMJJ_RS03090 0.23 +0.0 580,693 + MMJJ_RS03090 0.23 +0.7 580,729 - MMJJ_RS03090 0.29 +0.6 580,729 - MMJJ_RS03090 0.29 +0.6 580,729 - MMJJ_RS03090 0.29 +1.2 580,729 - MMJJ_RS03090 0.29 -0.5 580,729 - MMJJ_RS03090 0.29 +0.5 580,729 - MMJJ_RS03090 0.29 +1.7 580,729 - MMJJ_RS03090 0.29 -2.6 580,730 + MMJJ_RS03090 0.29 +0.3 580,730 + MMJJ_RS03090 0.29 +0.7 580,730 + MMJJ_RS03090 0.29 +0.2 580,730 + MMJJ_RS03090 0.29 -1.2 580,730 + MMJJ_RS03090 0.29 +0.2 580,731 - MMJJ_RS03090 0.29 +0.4 580,731 - MMJJ_RS03090 0.29 -0.2 580,731 - MMJJ_RS03090 0.29 -0.4 580,731 - MMJJ_RS03090 0.29 -0.1 580,731 - MMJJ_RS03090 0.29 +0.1 580,731 - MMJJ_RS03090 0.29 +0.0 580,732 + MMJJ_RS03090 0.29 -0.2 580,732 + MMJJ_RS03090 0.29 -2.4 580,737 - MMJJ_RS03090 0.30 +0.0 580,737 - MMJJ_RS03090 0.30 -0.0 580,738 + MMJJ_RS03090 0.30 -0.1 580,738 + MMJJ_RS03090 0.30 +1.0 580,738 + MMJJ_RS03090 0.30 -0.4 580,738 + MMJJ_RS03090 0.30 +0.0 580,761 + MMJJ_RS03090 0.34 -1.8 580,777 - MMJJ_RS03090 0.37 +0.2 580,777 - MMJJ_RS03090 0.37 -1.6 580,778 + MMJJ_RS03090 0.37 +0.4 580,781 + MMJJ_RS03090 0.38 +0.2 580,781 + MMJJ_RS03090 0.38 -0.8 580,789 + MMJJ_RS03090 0.39 +1.6 580,789 + MMJJ_RS03090 0.39 +0.2 580,789 + MMJJ_RS03090 0.39 +0.6 580,789 + MMJJ_RS03090 0.39 +1.0 580,790 - MMJJ_RS03090 0.39 +1.3 580,790 - MMJJ_RS03090 0.39 +0.3 580,791 + MMJJ_RS03090 0.39 +0.7 580,791 + MMJJ_RS03090 0.39 -0.0 580,834 + MMJJ_RS03090 0.46 +0.2 580,834 + MMJJ_RS03090 0.46 +0.3 580,835 - MMJJ_RS03090 0.47 +0.4 580,843 - MMJJ_RS03090 0.48 -0.3 580,844 + MMJJ_RS03090 0.48 -0.6 580,874 - MMJJ_RS03090 0.53 +0.3 580,908 - MMJJ_RS03090 0.59 -0.3 580,909 + MMJJ_RS03090 0.59 -0.3 580,918 - MMJJ_RS03090 0.60 -1.7 580,918 - MMJJ_RS03090 0.60 +0.5 580,919 + MMJJ_RS03090 0.61 +0.6 580,922 + MMJJ_RS03090 0.61 +0.4 580,922 + MMJJ_RS03090 0.61 +0.6 580,933 - MMJJ_RS03090 0.63 +0.2 580,933 - MMJJ_RS03090 0.63 +0.3 581,015 - MMJJ_RS03090 0.77 +0.6 581,015 - MMJJ_RS03090 0.77 -0.7 581,016 + MMJJ_RS03090 0.77 -0.2 581,016 + MMJJ_RS03090 0.77 -0.1 581,028 + MMJJ_RS03090 0.79 +0.2 581,028 + MMJJ_RS03090 0.79 +0.1 581,031 - MMJJ_RS03090 0.79 -0.7 581,053 - MMJJ_RS03090 0.83 -0.5 581,053 - MMJJ_RS03090 0.83 +0.4 581,054 + MMJJ_RS03090 0.83 -1.3 581,054 + MMJJ_RS03090 0.83 -1.4 581,054 + MMJJ_RS03090 0.83 +0.3 581,058 + MMJJ_RS03090 0.84 +0.0 581,074 + MMJJ_RS03090 0.87 +0.2 581,074 + MMJJ_RS03090 0.87 +1.0 581,107 - -0.6 581,142 - +0.2 581,153 - +0.9 581,153 - +0.5 581,154 + +0.1 581,156 - +0.1 581,156 - +0.3 581,157 + +0.9 581,162 + -0.2 581,162 + -1.3 581,172 - -0.2 581,173 + +0.0 581,184 + +0.0 581,197 - -0.6 581,197 - +0.1 581,197 - +0.6 581,198 + +0.2 581,198 + +0.8 581,198 + +0.5 581,201 - -0.6 581,201 - -0.5 581,201 - -1.4 581,202 + -1.2 581,202 + +0.2 581,202 + -1.3 581,202 + +0.7 581,203 - -1.5 581,206 + +0.5 581,230 + +0.4 581,248 - -2.2 581,249 + -1.2 581,249 + -1.3 581,249 + -0.2 581,249 + -1.6 581,249 + -1.6 581,306 + -1.8 581,330 - -1.3 581,415 - MMJJ_RS03095 0.11 -2.3 582,140 - MMJJ_RS03095 0.55 -1.0 582,143 - MMJJ_RS03095 0.55 -3.0 582,335 + MMJJ_RS03095 0.67 -2.0 582,484 + MMJJ_RS03095 0.76 -1.8 582,487 - MMJJ_RS03095 0.76 -1.8 582,946 + -2.1 582,964 - -1.6 583,003 + -2.6 583,018 - -1.8 583,041 - -3.1 583,520 - MMJJ_RS03100 0.25 +1.0
Or see this region's nucleotide sequence