Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02995 and MMJJ_RS03000 are separated by 55 nucleotides MMJJ_RS03000 and MMJJ_RS03005 are separated by 260 nucleotides MMJJ_RS03005 and MMJJ_RS03010 are separated by 89 nucleotides
MMJJ_RS02995: MMJJ_RS02995 - RNase P subunit p30 family protein, at 560,763 to 561,461
_RS02995
MMJJ_RS03000: MMJJ_RS03000 - DUF211 domain-containing protein, at 561,517 to 561,810
_RS03000
MMJJ_RS03005: MMJJ_RS03005 - Ni-sirohydrochlorin a,c-diamide reductive cyclase catalytic subunit, at 562,071 to 563,171
_RS03005
MMJJ_RS03010: MMJJ_RS03010 - replication factor C small subunit, at 563,261 to 564,208
_RS03010
Position (kb) 562
563
564 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 561.183 kb on - strand, within MMJJ_RS02995 at 561.353 kb on + strand, within MMJJ_RS02995 at 561.483 kb on + strand at 561.493 kb on + strand at 561.518 kb on - strand at 561.518 kb on - strand at 561.706 kb on - strand, within MMJJ_RS03000 at 561.707 kb on + strand, within MMJJ_RS03000 at 561.886 kb on - strand at 561.886 kb on - strand at 561.889 kb on - strand at 561.890 kb on + strand at 561.894 kb on + strand at 561.895 kb on - strand at 561.896 kb on + strand at 561.899 kb on - strand at 561.899 kb on - strand at 561.899 kb on - strand at 561.900 kb on + strand at 561.900 kb on + strand at 561.900 kb on + strand at 561.900 kb on + strand at 561.900 kb on + strand at 561.900 kb on + strand at 561.900 kb on + strand at 561.901 kb on - strand at 561.901 kb on - strand at 561.901 kb on - strand at 561.902 kb on + strand at 561.902 kb on + strand at 561.907 kb on - strand at 561.920 kb on - strand at 561.920 kb on - strand at 561.920 kb on - strand at 561.921 kb on + strand at 561.921 kb on + strand at 561.922 kb on - strand at 561.922 kb on - strand at 561.922 kb on - strand at 561.924 kb on - strand at 561.925 kb on + strand at 561.925 kb on + strand at 561.925 kb on + strand at 561.925 kb on + strand at 561.927 kb on - strand at 561.927 kb on - strand at 561.928 kb on + strand at 561.944 kb on - strand at 561.944 kb on - strand at 561.944 kb on - strand at 561.945 kb on + strand at 561.956 kb on - strand at 561.957 kb on + strand at 561.967 kb on - strand at 561.972 kb on + strand at 561.985 kb on - strand at 562.032 kb on + strand at 562.045 kb on - strand at 562.078 kb on - strand at 562.277 kb on + strand, within MMJJ_RS03005 at 562.277 kb on + strand, within MMJJ_RS03005 at 562.412 kb on + strand, within MMJJ_RS03005 at 562.803 kb on + strand, within MMJJ_RS03005 at 562.820 kb on - strand, within MMJJ_RS03005 at 562.826 kb on - strand, within MMJJ_RS03005 at 562.866 kb on - strand, within MMJJ_RS03005 at 562.867 kb on + strand, within MMJJ_RS03005 at 563.027 kb on + strand, within MMJJ_RS03005 at 563.057 kb on + strand, within MMJJ_RS03005 at 563.069 kb on - strand at 563.070 kb on + strand at 563.197 kb on - strand at 563.198 kb on + strand at 563.826 kb on + strand, within MMJJ_RS03010 at 563.866 kb on + strand, within MMJJ_RS03010 at 564.083 kb on + strand, within MMJJ_RS03010
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 561,183 - MMJJ_RS02995 0.60 -2.1 561,353 + MMJJ_RS02995 0.84 -2.0 561,483 + +0.0 561,493 + -1.8 561,518 - -2.8 561,518 - -1.9 561,706 - MMJJ_RS03000 0.64 +1.8 561,707 + MMJJ_RS03000 0.65 -3.0 561,886 - -2.0 561,886 - -0.6 561,889 - -3.1 561,890 + -0.7 561,894 + -1.1 561,895 - -1.3 561,896 + +0.5 561,899 - -0.1 561,899 - -0.1 561,899 - -0.1 561,900 + -0.1 561,900 + +0.7 561,900 + +0.2 561,900 + -0.5 561,900 + +0.9 561,900 + +1.5 561,900 + +0.7 561,901 - +0.2 561,901 - +0.6 561,901 - +0.3 561,902 + +0.4 561,902 + -0.0 561,907 - -0.8 561,920 - +0.8 561,920 - +0.2 561,920 - +0.2 561,921 + -0.9 561,921 + -0.2 561,922 - +1.0 561,922 - +0.2 561,922 - +0.0 561,924 - +0.1 561,925 + +0.2 561,925 + -1.0 561,925 + -0.2 561,925 + +1.1 561,927 - +2.3 561,927 - +0.1 561,928 + -0.3 561,944 - -2.3 561,944 - -0.1 561,944 - -0.3 561,945 + +1.1 561,956 - -0.3 561,957 + +0.2 561,967 - +1.0 561,972 + +0.6 561,985 - +0.0 562,032 + +0.0 562,045 - -3.1 562,078 - -2.8 562,277 + MMJJ_RS03005 0.19 -2.6 562,277 + MMJJ_RS03005 0.19 -1.6 562,412 + MMJJ_RS03005 0.31 -2.0 562,803 + MMJJ_RS03005 0.66 -2.4 562,820 - MMJJ_RS03005 0.68 -1.6 562,826 - MMJJ_RS03005 0.69 -3.6 562,866 - MMJJ_RS03005 0.72 -2.0 562,867 + MMJJ_RS03005 0.72 -2.9 563,027 + MMJJ_RS03005 0.87 -0.6 563,057 + MMJJ_RS03005 0.90 -1.3 563,069 - -3.0 563,070 + -1.0 563,197 - -0.6 563,198 + +0.4 563,826 + MMJJ_RS03010 0.60 -2.6 563,866 + MMJJ_RS03010 0.64 -2.0 564,083 + MMJJ_RS03010 0.87 -0.6
Or see this region's nucleotide sequence