Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02080 and MMJJ_RS02085 are separated by 41 nucleotides MMJJ_RS02085 and MMJJ_RS02090 are separated by 19 nucleotides MMJJ_RS02090 and MMJJ_RS02095 are separated by 64 nucleotides MMJJ_RS02095 and MMJJ_RS02100 are separated by 151 nucleotides
MMJJ_RS02080: MMJJ_RS02080 - AMP-binding protein, at 395,534 to 397,183
_RS02080
MMJJ_RS02085: MMJJ_RS02085 - hypothetical protein, at 397,225 to 398,148
_RS02085
MMJJ_RS02090: MMJJ_RS02090 - tyramine--L-glutamate ligase, at 398,168 to 399,076
_RS02090
MMJJ_RS02095: MMJJ_RS02095 - DUF2124 family protein, at 399,141 to 399,623
_RS02095
MMJJ_RS02100: MMJJ_RS02100 - hypothetical protein, at 399,775 to 400,065
_RS02100
Position (kb) 398
399
400 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 397.180 kb on + strand at 397.181 kb on - strand at 397.181 kb on - strand at 397.182 kb on + strand at 397.182 kb on + strand at 397.182 kb on + strand at 397.186 kb on - strand at 397.213 kb on + strand at 397.213 kb on + strand at 397.224 kb on - strand at 397.224 kb on - strand at 397.225 kb on + strand at 397.232 kb on + strand at 397.277 kb on - strand at 397.278 kb on + strand at 397.278 kb on + strand at 397.289 kb on - strand at 397.289 kb on - strand at 397.312 kb on + strand at 397.386 kb on + strand, within MMJJ_RS02085 at 397.387 kb on - strand, within MMJJ_RS02085 at 397.387 kb on - strand, within MMJJ_RS02085 at 397.388 kb on + strand, within MMJJ_RS02085 at 397.388 kb on + strand, within MMJJ_RS02085 at 397.388 kb on + strand, within MMJJ_RS02085 at 397.388 kb on + strand, within MMJJ_RS02085 at 397.389 kb on - strand, within MMJJ_RS02085 at 397.389 kb on - strand, within MMJJ_RS02085 at 397.390 kb on + strand, within MMJJ_RS02085 at 397.390 kb on + strand, within MMJJ_RS02085 at 397.390 kb on + strand, within MMJJ_RS02085 at 397.418 kb on - strand, within MMJJ_RS02085 at 397.418 kb on - strand, within MMJJ_RS02085 at 397.422 kb on + strand, within MMJJ_RS02085 at 397.451 kb on - strand, within MMJJ_RS02085 at 397.507 kb on - strand, within MMJJ_RS02085 at 397.507 kb on - strand, within MMJJ_RS02085 at 397.508 kb on + strand, within MMJJ_RS02085 at 397.516 kb on + strand, within MMJJ_RS02085 at 397.560 kb on + strand, within MMJJ_RS02085 at 397.578 kb on - strand, within MMJJ_RS02085 at 397.609 kb on + strand, within MMJJ_RS02085 at 397.610 kb on - strand, within MMJJ_RS02085 at 397.610 kb on - strand, within MMJJ_RS02085 at 397.611 kb on + strand, within MMJJ_RS02085 at 397.611 kb on + strand, within MMJJ_RS02085 at 397.611 kb on + strand, within MMJJ_RS02085 at 397.622 kb on + strand, within MMJJ_RS02085 at 397.646 kb on - strand, within MMJJ_RS02085 at 397.647 kb on + strand, within MMJJ_RS02085 at 397.697 kb on - strand, within MMJJ_RS02085 at 397.698 kb on + strand, within MMJJ_RS02085 at 397.707 kb on - strand, within MMJJ_RS02085 at 397.781 kb on - strand, within MMJJ_RS02085 at 397.833 kb on + strand, within MMJJ_RS02085 at 397.864 kb on - strand, within MMJJ_RS02085 at 397.864 kb on - strand, within MMJJ_RS02085 at 397.864 kb on - strand, within MMJJ_RS02085 at 397.864 kb on - strand, within MMJJ_RS02085 at 397.865 kb on + strand, within MMJJ_RS02085 at 397.892 kb on - strand, within MMJJ_RS02085 at 397.892 kb on - strand, within MMJJ_RS02085 at 397.896 kb on + strand, within MMJJ_RS02085 at 397.907 kb on - strand, within MMJJ_RS02085 at 397.907 kb on - strand, within MMJJ_RS02085 at 397.943 kb on - strand, within MMJJ_RS02085 at 397.943 kb on - strand, within MMJJ_RS02085 at 397.947 kb on + strand, within MMJJ_RS02085 at 397.971 kb on - strand, within MMJJ_RS02085 at 397.971 kb on - strand, within MMJJ_RS02085 at 397.971 kb on - strand, within MMJJ_RS02085 at 397.971 kb on - strand, within MMJJ_RS02085 at 397.999 kb on + strand, within MMJJ_RS02085 at 398.007 kb on + strand, within MMJJ_RS02085 at 398.034 kb on - strand, within MMJJ_RS02085 at 398.034 kb on - strand, within MMJJ_RS02085 at 398.048 kb on - strand, within MMJJ_RS02085 at 398.110 kb on + strand at 398.184 kb on - strand at 398.261 kb on + strand, within MMJJ_RS02090 at 398.268 kb on - strand, within MMJJ_RS02090 at 398.374 kb on + strand, within MMJJ_RS02090 at 398.458 kb on + strand, within MMJJ_RS02090 at 398.463 kb on + strand, within MMJJ_RS02090 at 398.479 kb on + strand, within MMJJ_RS02090 at 398.527 kb on + strand, within MMJJ_RS02090 at 398.556 kb on + strand, within MMJJ_RS02090 at 398.667 kb on + strand, within MMJJ_RS02090 at 398.752 kb on - strand, within MMJJ_RS02090 at 398.753 kb on + strand at 398.795 kb on + strand, within MMJJ_RS02090 at 398.804 kb on + strand, within MMJJ_RS02090 at 398.845 kb on - strand, within MMJJ_RS02090 at 398.916 kb on - strand, within MMJJ_RS02090 at 399.100 kb on + strand at 399.109 kb on + strand at 399.113 kb on - strand at 399.113 kb on - strand at 399.152 kb on + strand at 399.192 kb on + strand, within MMJJ_RS02095 at 399.226 kb on - strand, within MMJJ_RS02095 at 399.288 kb on + strand, within MMJJ_RS02095 at 399.309 kb on - strand, within MMJJ_RS02095 at 399.310 kb on + strand, within MMJJ_RS02095 at 399.314 kb on - strand, within MMJJ_RS02095 at 399.314 kb on - strand, within MMJJ_RS02095 at 399.357 kb on + strand, within MMJJ_RS02095 at 399.357 kb on + strand, within MMJJ_RS02095 at 399.408 kb on + strand, within MMJJ_RS02095 at 399.475 kb on - strand, within MMJJ_RS02095 at 399.484 kb on - strand, within MMJJ_RS02095 at 399.489 kb on + strand, within MMJJ_RS02095 at 399.515 kb on + strand, within MMJJ_RS02095 at 399.515 kb on + strand, within MMJJ_RS02095 at 399.520 kb on - strand, within MMJJ_RS02095 at 399.520 kb on - strand, within MMJJ_RS02095 at 399.520 kb on - strand, within MMJJ_RS02095 at 399.521 kb on + strand, within MMJJ_RS02095 at 399.535 kb on - strand, within MMJJ_RS02095 at 399.576 kb on + strand at 399.580 kb on - strand at 399.584 kb on + strand at 399.584 kb on + strand at 399.625 kb on - strand at 399.654 kb on + strand at 399.711 kb on - strand at 399.711 kb on - strand at 399.711 kb on - strand at 399.711 kb on - strand at 399.711 kb on - strand at 399.712 kb on + strand at 399.716 kb on + strand at 399.716 kb on + strand at 399.737 kb on + strand at 399.741 kb on + strand at 399.741 kb on + strand at 399.742 kb on - strand at 399.742 kb on - strand at 399.742 kb on - strand at 399.743 kb on + strand at 399.804 kb on + strand, within MMJJ_RS02100 at 399.817 kb on - strand, within MMJJ_RS02100 at 399.817 kb on - strand, within MMJJ_RS02100 at 399.822 kb on - strand, within MMJJ_RS02100 at 399.822 kb on - strand, within MMJJ_RS02100 at 399.823 kb on + strand, within MMJJ_RS02100 at 399.823 kb on + strand, within MMJJ_RS02100 at 399.823 kb on + strand, within MMJJ_RS02100 at 399.824 kb on - strand, within MMJJ_RS02100 at 399.825 kb on + strand, within MMJJ_RS02100 at 399.825 kb on + strand, within MMJJ_RS02100 at 399.825 kb on + strand, within MMJJ_RS02100 at 399.825 kb on + strand, within MMJJ_RS02100 at 399.825 kb on + strand, within MMJJ_RS02100 at 399.912 kb on + strand, within MMJJ_RS02100 at 399.954 kb on + strand, within MMJJ_RS02100 at 399.961 kb on - strand, within MMJJ_RS02100 at 399.962 kb on + strand, within MMJJ_RS02100 at 399.962 kb on + strand, within MMJJ_RS02100 at 399.965 kb on + strand, within MMJJ_RS02100 at 399.965 kb on + strand, within MMJJ_RS02100 at 399.987 kb on - strand, within MMJJ_RS02100 at 399.993 kb on + strand, within MMJJ_RS02100 at 400.022 kb on + strand, within MMJJ_RS02100 at 400.022 kb on + strand, within MMJJ_RS02100
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 397,180 + +0.2 397,181 - -0.3 397,181 - +0.8 397,182 + +0.0 397,182 + +0.1 397,182 + +0.2 397,186 - -0.3 397,213 + +0.2 397,213 + +2.0 397,224 - -2.0 397,224 - +0.6 397,225 + +0.6 397,232 + +0.2 397,277 - +0.1 397,278 + +0.4 397,278 + +0.1 397,289 - +0.7 397,289 - +0.2 397,312 + -0.6 397,386 + MMJJ_RS02085 0.17 -0.2 397,387 - MMJJ_RS02085 0.18 -0.1 397,387 - MMJJ_RS02085 0.18 +1.0 397,388 + MMJJ_RS02085 0.18 +0.3 397,388 + MMJJ_RS02085 0.18 -3.4 397,388 + MMJJ_RS02085 0.18 +0.3 397,388 + MMJJ_RS02085 0.18 -1.0 397,389 - MMJJ_RS02085 0.18 +1.1 397,389 - MMJJ_RS02085 0.18 +0.3 397,390 + MMJJ_RS02085 0.18 +0.6 397,390 + MMJJ_RS02085 0.18 -0.4 397,390 + MMJJ_RS02085 0.18 -1.0 397,418 - MMJJ_RS02085 0.21 -1.1 397,418 - MMJJ_RS02085 0.21 +0.3 397,422 + MMJJ_RS02085 0.21 -0.4 397,451 - MMJJ_RS02085 0.24 -0.3 397,507 - MMJJ_RS02085 0.31 -0.3 397,507 - MMJJ_RS02085 0.31 -0.0 397,508 + MMJJ_RS02085 0.31 +0.2 397,516 + MMJJ_RS02085 0.31 -0.7 397,560 + MMJJ_RS02085 0.36 +0.0 397,578 - MMJJ_RS02085 0.38 -1.2 397,609 + MMJJ_RS02085 0.42 +1.7 397,610 - MMJJ_RS02085 0.42 +0.0 397,610 - MMJJ_RS02085 0.42 -0.6 397,611 + MMJJ_RS02085 0.42 -0.3 397,611 + MMJJ_RS02085 0.42 +0.2 397,611 + MMJJ_RS02085 0.42 -1.3 397,622 + MMJJ_RS02085 0.43 +0.2 397,646 - MMJJ_RS02085 0.46 -0.9 397,647 + MMJJ_RS02085 0.46 +0.5 397,697 - MMJJ_RS02085 0.51 +0.0 397,698 + MMJJ_RS02085 0.51 +0.5 397,707 - MMJJ_RS02085 0.52 +0.3 397,781 - MMJJ_RS02085 0.60 -0.7 397,833 + MMJJ_RS02085 0.66 +0.3 397,864 - MMJJ_RS02085 0.69 -0.1 397,864 - MMJJ_RS02085 0.69 -2.8 397,864 - MMJJ_RS02085 0.69 +0.2 397,864 - MMJJ_RS02085 0.69 +0.4 397,865 + MMJJ_RS02085 0.69 +0.0 397,892 - MMJJ_RS02085 0.72 -0.2 397,892 - MMJJ_RS02085 0.72 -0.0 397,896 + MMJJ_RS02085 0.73 -0.3 397,907 - MMJJ_RS02085 0.74 +0.3 397,907 - MMJJ_RS02085 0.74 -0.7 397,943 - MMJJ_RS02085 0.78 +0.2 397,943 - MMJJ_RS02085 0.78 -0.4 397,947 + MMJJ_RS02085 0.78 -0.5 397,971 - MMJJ_RS02085 0.81 +0.9 397,971 - MMJJ_RS02085 0.81 +0.6 397,971 - MMJJ_RS02085 0.81 +0.5 397,971 - MMJJ_RS02085 0.81 -2.1 397,999 + MMJJ_RS02085 0.84 -0.3 398,007 + MMJJ_RS02085 0.85 +0.0 398,034 - MMJJ_RS02085 0.88 +0.0 398,034 - MMJJ_RS02085 0.88 +1.0 398,048 - MMJJ_RS02085 0.89 +0.0 398,110 + -1.7 398,184 - -3.0 398,261 + MMJJ_RS02090 0.10 -3.1 398,268 - MMJJ_RS02090 0.11 -3.1 398,374 + MMJJ_RS02090 0.23 -3.8 398,458 + MMJJ_RS02090 0.32 -2.6 398,463 + MMJJ_RS02090 0.32 -2.0 398,479 + MMJJ_RS02090 0.34 -4.5 398,527 + MMJJ_RS02090 0.39 -2.9 398,556 + MMJJ_RS02090 0.43 -2.1 398,667 + MMJJ_RS02090 0.55 -2.0 398,752 - MMJJ_RS02090 0.64 -3.5 398,753 + -1.8 398,795 + MMJJ_RS02090 0.69 -1.8 398,804 + MMJJ_RS02090 0.70 -2.1 398,845 - MMJJ_RS02090 0.74 -2.1 398,916 - MMJJ_RS02090 0.82 -2.6 399,100 + +1.2 399,109 + -0.6 399,113 - -0.4 399,113 - +0.2 399,152 + -0.6 399,192 + MMJJ_RS02095 0.11 -0.1 399,226 - MMJJ_RS02095 0.18 +0.0 399,288 + MMJJ_RS02095 0.30 +0.4 399,309 - MMJJ_RS02095 0.35 +0.8 399,310 + MMJJ_RS02095 0.35 +0.9 399,314 - MMJJ_RS02095 0.36 +0.2 399,314 - MMJJ_RS02095 0.36 -0.8 399,357 + MMJJ_RS02095 0.45 +0.3 399,357 + MMJJ_RS02095 0.45 +0.8 399,408 + MMJJ_RS02095 0.55 +0.8 399,475 - MMJJ_RS02095 0.69 -0.3 399,484 - MMJJ_RS02095 0.71 -1.3 399,489 + MMJJ_RS02095 0.72 +0.0 399,515 + MMJJ_RS02095 0.77 -3.0 399,515 + MMJJ_RS02095 0.77 +0.0 399,520 - MMJJ_RS02095 0.78 +0.5 399,520 - MMJJ_RS02095 0.78 -0.1 399,520 - MMJJ_RS02095 0.78 -0.6 399,521 + MMJJ_RS02095 0.79 -0.4 399,535 - MMJJ_RS02095 0.82 -0.6 399,576 + +0.8 399,580 - +1.2 399,584 + +0.5 399,584 + -0.6 399,625 - -0.4 399,654 + +0.5 399,711 - -0.4 399,711 - -0.1 399,711 - -0.1 399,711 - +1.2 399,711 - +0.6 399,712 + +0.6 399,716 + +0.2 399,716 + +0.0 399,737 + -0.0 399,741 + -0.1 399,741 + -1.9 399,742 - +0.0 399,742 - +0.1 399,742 - +0.2 399,743 + -0.3 399,804 + MMJJ_RS02100 0.10 -1.1 399,817 - MMJJ_RS02100 0.14 -0.4 399,817 - MMJJ_RS02100 0.14 +0.3 399,822 - MMJJ_RS02100 0.16 +1.0 399,822 - MMJJ_RS02100 0.16 +0.1 399,823 + MMJJ_RS02100 0.16 +0.2 399,823 + MMJJ_RS02100 0.16 -0.1 399,823 + MMJJ_RS02100 0.16 +0.0 399,824 - MMJJ_RS02100 0.17 +0.3 399,825 + MMJJ_RS02100 0.17 +0.6 399,825 + MMJJ_RS02100 0.17 +0.6 399,825 + MMJJ_RS02100 0.17 +0.6 399,825 + MMJJ_RS02100 0.17 -0.1 399,825 + MMJJ_RS02100 0.17 +0.6 399,912 + MMJJ_RS02100 0.47 +0.1 399,954 + MMJJ_RS02100 0.62 +0.1 399,961 - MMJJ_RS02100 0.64 +0.5 399,962 + MMJJ_RS02100 0.64 -0.3 399,962 + MMJJ_RS02100 0.64 +0.1 399,965 + MMJJ_RS02100 0.65 -0.4 399,965 + MMJJ_RS02100 0.65 +0.1 399,987 - MMJJ_RS02100 0.73 +0.2 399,993 + MMJJ_RS02100 0.75 +0.3 400,022 + MMJJ_RS02100 0.85 -0.0 400,022 + MMJJ_RS02100 0.85 -1.6
Or see this region's nucleotide sequence