Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02035 and MMJJ_RS02040 are separated by 17 nucleotides MMJJ_RS02040 and MMJJ_RS02045 are separated by 542 nucleotides MMJJ_RS02045 and MMJJ_RS02050 are separated by 110 nucleotides MMJJ_RS02050 and MMJJ_RS02055 are separated by 132 nucleotides
MMJJ_RS02035: MMJJ_RS02035 - 4-hydroxy-tetrahydrodipicolinate synthase, at 388,049 to 388,918
_RS02035
MMJJ_RS02040: MMJJ_RS02040 - Asp-tRNA(Asn) amidotransferase subunit GatC, at 388,936 to 389,175
_RS02040
MMJJ_RS02045: MMJJ_RS02045 - molybdenum cofactor guanylyltransferase, at 389,718 to 390,362
_RS02045
MMJJ_RS02050: MMJJ_RS02050 - peptidylprolyl isomerase, at 390,473 to 391,159
_RS02050
MMJJ_RS02055: MMJJ_RS02055 - GTP 3',8-cyclase MoaA, at 391,292 to 392,185
_RS02055
Position (kb)
389
390
391 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 389.080 kb on + strand, within MMJJ_RS02040 at 389.111 kb on - strand, within MMJJ_RS02040 at 389.125 kb on + strand, within MMJJ_RS02040 at 389.209 kb on + strand at 389.262 kb on + strand at 389.309 kb on - strand at 389.309 kb on - strand at 389.309 kb on - strand at 389.313 kb on - strand at 389.313 kb on - strand at 389.314 kb on + strand at 389.321 kb on - strand at 389.325 kb on - strand at 389.351 kb on - strand at 389.351 kb on - strand at 389.352 kb on + strand at 389.352 kb on + strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.357 kb on - strand at 389.357 kb on - strand at 389.357 kb on - strand at 389.358 kb on + strand at 389.358 kb on + strand at 389.358 kb on + strand at 389.369 kb on - strand at 389.375 kb on - strand at 389.375 kb on - strand at 389.381 kb on - strand at 389.398 kb on + strand at 389.450 kb on - strand at 389.450 kb on - strand at 389.451 kb on + strand at 389.451 kb on + strand at 389.452 kb on - strand at 389.453 kb on + strand at 389.453 kb on + strand at 389.454 kb on - strand at 389.455 kb on + strand at 389.477 kb on + strand at 389.482 kb on - strand at 389.483 kb on + strand at 389.483 kb on + strand at 389.483 kb on + strand at 389.490 kb on - strand at 389.490 kb on - strand at 389.524 kb on - strand at 389.569 kb on - strand at 389.588 kb on - strand at 389.588 kb on - strand at 389.589 kb on + strand at 389.589 kb on + strand at 389.592 kb on - strand at 389.609 kb on + strand at 389.618 kb on - strand at 389.626 kb on + strand at 389.626 kb on + strand at 389.630 kb on + strand at 389.630 kb on + strand at 389.630 kb on + strand at 389.630 kb on + strand at 389.631 kb on - strand at 389.676 kb on - strand at 389.677 kb on + strand at 389.679 kb on - strand at 389.680 kb on + strand at 389.686 kb on - strand at 389.773 kb on + strand at 389.810 kb on + strand, within MMJJ_RS02045 at 389.892 kb on - strand, within MMJJ_RS02045 at 389.980 kb on + strand, within MMJJ_RS02045 at 390.012 kb on - strand, within MMJJ_RS02045 at 390.163 kb on - strand, within MMJJ_RS02045 at 390.167 kb on + strand, within MMJJ_RS02045 at 390.362 kb on - strand at 390.362 kb on - strand at 390.400 kb on - strand at 390.400 kb on - strand at 390.400 kb on - strand at 390.401 kb on + strand at 390.401 kb on + strand at 390.401 kb on + strand at 390.401 kb on + strand at 390.402 kb on - strand at 390.404 kb on - strand at 390.404 kb on - strand at 390.405 kb on + strand at 390.405 kb on + strand at 390.405 kb on + strand at 390.413 kb on + strand at 390.417 kb on + strand at 390.418 kb on - strand at 390.418 kb on - strand at 390.430 kb on - strand at 390.449 kb on - strand at 390.449 kb on - strand at 390.450 kb on + strand at 390.493 kb on + strand at 390.504 kb on + strand at 390.513 kb on + strand at 390.590 kb on - strand, within MMJJ_RS02050 at 390.767 kb on + strand, within MMJJ_RS02050 at 390.825 kb on - strand, within MMJJ_RS02050 at 390.826 kb on + strand, within MMJJ_RS02050 at 390.847 kb on + strand, within MMJJ_RS02050 at 390.849 kb on - strand, within MMJJ_RS02050 at 390.850 kb on + strand, within MMJJ_RS02050 at 390.850 kb on + strand, within MMJJ_RS02050 at 390.939 kb on - strand, within MMJJ_RS02050 at 390.939 kb on - strand, within MMJJ_RS02050 at 390.984 kb on - strand, within MMJJ_RS02050 at 390.990 kb on - strand, within MMJJ_RS02050 at 390.990 kb on - strand, within MMJJ_RS02050 at 391.011 kb on - strand, within MMJJ_RS02050 at 391.011 kb on - strand, within MMJJ_RS02050 at 391.017 kb on - strand, within MMJJ_RS02050 at 391.042 kb on - strand, within MMJJ_RS02050 at 391.042 kb on - strand, within MMJJ_RS02050 at 391.042 kb on - strand, within MMJJ_RS02050 at 391.043 kb on + strand, within MMJJ_RS02050 at 391.043 kb on + strand, within MMJJ_RS02050 at 391.171 kb on - strand at 391.171 kb on - strand at 391.172 kb on + strand at 391.192 kb on - strand at 391.193 kb on + strand at 391.193 kb on + strand at 391.251 kb on + strand at 391.287 kb on - strand at 391.346 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 389,080 + MMJJ_RS02040 0.60 -2.5 389,111 - MMJJ_RS02040 0.73 -2.3 389,125 + MMJJ_RS02040 0.79 -1.8 389,209 + -0.6 389,262 + -2.2 389,309 - +1.0 389,309 - -1.0 389,309 - +0.6 389,313 - -0.4 389,313 - +0.2 389,314 + -0.6 389,321 - +0.3 389,325 - +1.1 389,351 - +1.7 389,351 - +0.4 389,352 + +0.5 389,352 + +0.6 389,353 - -0.0 389,353 - +0.3 389,353 - -0.8 389,353 - +0.3 389,353 - -2.6 389,353 - +0.4 389,353 - -0.0 389,354 + -0.7 389,354 + +0.0 389,354 + +0.7 389,354 + -0.1 389,354 + -0.2 389,354 + -0.6 389,354 + -0.1 389,354 + -0.5 389,354 + +1.2 389,354 + +0.8 389,354 + +0.3 389,354 + -0.8 389,357 - -1.8 389,357 - +0.5 389,357 - +0.7 389,358 + -0.2 389,358 + -0.2 389,358 + +0.1 389,369 - +0.7 389,375 - -0.4 389,375 - -0.3 389,381 - +0.4 389,398 + -1.8 389,450 - -0.7 389,450 - +1.4 389,451 + +1.0 389,451 + +0.3 389,452 - -1.6 389,453 + -0.9 389,453 + +1.1 389,454 - +0.1 389,455 + +1.0 389,477 + -0.1 389,482 - -0.1 389,483 + -0.6 389,483 + +0.5 389,483 + -0.7 389,490 - -0.7 389,490 - +0.7 389,524 - +0.7 389,569 - +0.8 389,588 - -0.5 389,588 - +0.8 389,589 + +0.3 389,589 + +0.5 389,592 - +0.4 389,609 + -0.5 389,618 - -0.8 389,626 + -2.4 389,626 + +1.0 389,630 + -0.6 389,630 + -0.1 389,630 + +0.7 389,630 + +1.0 389,631 - +0.5 389,676 - +0.2 389,677 + -0.2 389,679 - +0.5 389,680 + +0.7 389,686 - +0.8 389,773 + -0.2 389,810 + MMJJ_RS02045 0.14 -3.7 389,892 - MMJJ_RS02045 0.27 -1.6 389,980 + MMJJ_RS02045 0.41 -3.7 390,012 - MMJJ_RS02045 0.46 -2.2 390,163 - MMJJ_RS02045 0.69 -1.0 390,167 + MMJJ_RS02045 0.70 -4.2 390,362 - -1.3 390,362 - -2.3 390,400 - -0.0 390,400 - -0.4 390,400 - +0.1 390,401 + -0.5 390,401 + -1.4 390,401 + +0.5 390,401 + -0.6 390,402 - -0.8 390,404 - -0.5 390,404 - -1.1 390,405 + +0.0 390,405 + -1.3 390,405 + +0.4 390,413 + -1.0 390,417 + -0.4 390,418 - -1.0 390,418 - +0.0 390,430 - +0.6 390,449 - +0.0 390,449 - +0.3 390,450 + +0.1 390,493 + -0.8 390,504 + +0.6 390,513 + +0.3 390,590 - MMJJ_RS02050 0.17 -0.3 390,767 + MMJJ_RS02050 0.43 +1.3 390,825 - MMJJ_RS02050 0.51 -0.2 390,826 + MMJJ_RS02050 0.51 -0.6 390,847 + MMJJ_RS02050 0.54 -0.2 390,849 - MMJJ_RS02050 0.55 +0.3 390,850 + MMJJ_RS02050 0.55 +1.8 390,850 + MMJJ_RS02050 0.55 -1.0 390,939 - MMJJ_RS02050 0.68 +1.8 390,939 - MMJJ_RS02050 0.68 +0.5 390,984 - MMJJ_RS02050 0.74 +0.2 390,990 - MMJJ_RS02050 0.75 -0.3 390,990 - MMJJ_RS02050 0.75 -0.0 391,011 - MMJJ_RS02050 0.78 +0.7 391,011 - MMJJ_RS02050 0.78 +0.4 391,017 - MMJJ_RS02050 0.79 +0.4 391,042 - MMJJ_RS02050 0.83 +0.3 391,042 - MMJJ_RS02050 0.83 -0.2 391,042 - MMJJ_RS02050 0.83 +0.0 391,043 + MMJJ_RS02050 0.83 +0.0 391,043 + MMJJ_RS02050 0.83 -0.1 391,171 - +0.5 391,171 - +1.2 391,172 + +1.4 391,192 - +1.3 391,193 + -0.3 391,193 + -0.3 391,251 + -1.3 391,287 - -2.3 391,346 + -2.8
Or see this region's nucleotide sequence