Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01870 and MMJJ_RS01875 are separated by 36 nucleotides MMJJ_RS01875 and MMJJ_RS01880 are separated by 22 nucleotides MMJJ_RS01880 and MMJJ_RS01885 are separated by 52 nucleotides
MMJJ_RS01870: MMJJ_RS01870 - methanogenesis marker 15 protein, at 358,786 to 360,021
_RS01870
MMJJ_RS01875: MMJJ_RS01875 - global nitrogen regulator NrpR, at 360,058 to 361,668
_RS01875
MMJJ_RS01880: MMJJ_RS01880 - RlmE family RNA methyltransferase, at 361,691 to 362,467
_RS01880
MMJJ_RS01885: MMJJ_RS01885 - KH domain-containing protein, at 362,520 to 363,074
_RS01885
Position (kb) 360
361
362 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 359.061 kb on + strand at 359.082 kb on + strand, within MMJJ_RS01870 at 359.528 kb on - strand, within MMJJ_RS01870 at 359.528 kb on - strand, within MMJJ_RS01870 at 359.733 kb on + strand, within MMJJ_RS01870 at 359.855 kb on - strand, within MMJJ_RS01870 at 359.856 kb on + strand at 359.965 kb on + strand at 360.045 kb on - strand at 360.045 kb on - strand at 360.046 kb on + strand at 360.046 kb on + strand at 360.046 kb on + strand at 360.046 kb on + strand at 360.046 kb on + strand at 360.158 kb on + strand at 360.191 kb on - strand at 360.294 kb on - strand, within MMJJ_RS01875 at 360.414 kb on + strand, within MMJJ_RS01875 at 360.437 kb on + strand at 360.477 kb on - strand, within MMJJ_RS01875 at 360.477 kb on - strand, within MMJJ_RS01875 at 360.478 kb on + strand, within MMJJ_RS01875 at 360.488 kb on - strand, within MMJJ_RS01875 at 360.501 kb on - strand, within MMJJ_RS01875 at 360.501 kb on - strand, within MMJJ_RS01875 at 360.540 kb on + strand, within MMJJ_RS01875 at 360.540 kb on + strand, within MMJJ_RS01875 at 360.651 kb on - strand, within MMJJ_RS01875 at 360.675 kb on + strand, within MMJJ_RS01875 at 360.900 kb on - strand, within MMJJ_RS01875 at 360.911 kb on - strand, within MMJJ_RS01875 at 360.912 kb on + strand, within MMJJ_RS01875 at 360.912 kb on + strand, within MMJJ_RS01875 at 361.046 kb on - strand, within MMJJ_RS01875 at 361.085 kb on - strand at 361.112 kb on + strand, within MMJJ_RS01875 at 361.245 kb on + strand at 361.449 kb on + strand, within MMJJ_RS01875 at 361.679 kb on + strand at 361.740 kb on + strand at 361.743 kb on + strand at 361.785 kb on + strand, within MMJJ_RS01880 at 361.848 kb on - strand, within MMJJ_RS01880 at 362.107 kb on - strand, within MMJJ_RS01880 at 362.278 kb on + strand, within MMJJ_RS01880 at 362.280 kb on + strand, within MMJJ_RS01880 at 362.340 kb on - strand, within MMJJ_RS01880 at 362.341 kb on + strand, within MMJJ_RS01880 at 362.478 kb on + strand at 362.481 kb on + strand at 362.482 kb on - strand at 362.491 kb on - strand at 362.491 kb on - strand at 362.492 kb on + strand at 362.505 kb on + strand at 362.516 kb on + strand at 362.516 kb on + strand at 362.522 kb on + strand at 362.535 kb on + strand at 362.550 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 359,061 + +1.0 359,082 + MMJJ_RS01870 0.24 -0.9 359,528 - MMJJ_RS01870 0.60 -1.3 359,528 - MMJJ_RS01870 0.60 -3.2 359,733 + MMJJ_RS01870 0.77 -1.3 359,855 - MMJJ_RS01870 0.86 -0.6 359,856 + -1.6 359,965 + -2.0 360,045 - +1.0 360,045 - -0.6 360,046 + -2.0 360,046 + -0.6 360,046 + -2.0 360,046 + -3.2 360,046 + -2.3 360,158 + -3.5 360,191 - -2.8 360,294 - MMJJ_RS01875 0.15 -0.8 360,414 + MMJJ_RS01875 0.22 -2.5 360,437 + -0.0 360,477 - MMJJ_RS01875 0.26 -2.2 360,477 - MMJJ_RS01875 0.26 -3.1 360,478 + MMJJ_RS01875 0.26 -1.3 360,488 - MMJJ_RS01875 0.27 -1.0 360,501 - MMJJ_RS01875 0.27 +0.4 360,501 - MMJJ_RS01875 0.27 -1.8 360,540 + MMJJ_RS01875 0.30 -0.6 360,540 + MMJJ_RS01875 0.30 -1.6 360,651 - MMJJ_RS01875 0.37 -3.2 360,675 + MMJJ_RS01875 0.38 -0.0 360,900 - MMJJ_RS01875 0.52 -2.7 360,911 - MMJJ_RS01875 0.53 -4.0 360,912 + MMJJ_RS01875 0.53 -2.6 360,912 + MMJJ_RS01875 0.53 -3.4 361,046 - MMJJ_RS01875 0.61 -2.9 361,085 - -1.3 361,112 + MMJJ_RS01875 0.65 -2.3 361,245 + -1.6 361,449 + MMJJ_RS01875 0.86 -1.1 361,679 + -3.5 361,740 + -2.8 361,743 + -3.2 361,785 + MMJJ_RS01880 0.12 -1.2 361,848 - MMJJ_RS01880 0.20 -2.9 362,107 - MMJJ_RS01880 0.54 -2.6 362,278 + MMJJ_RS01880 0.76 -2.7 362,280 + MMJJ_RS01880 0.76 -1.6 362,340 - MMJJ_RS01880 0.84 -3.2 362,341 + MMJJ_RS01880 0.84 -1.6 362,478 + -0.2 362,481 + +1.0 362,482 - +0.6 362,491 - -0.0 362,491 - +1.0 362,492 + +0.6 362,505 + +0.7 362,516 + -1.2 362,516 + +0.3 362,522 + -0.2 362,535 + -0.2 362,550 + -1.1
Or see this region's nucleotide sequence