Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01355 and MMJJ_RS01360 are separated by 4 nucleotides MMJJ_RS01360 and MMJJ_RS01365 are separated by 92 nucleotides
MMJJ_RS01355: MMJJ_RS01355 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 261,551 to 262,834
_RS01355
MMJJ_RS01360: MMJJ_RS01360 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 262,839 to 263,939
_RS01360
MMJJ_RS01365: MMJJ_RS01365 - Mrp/NBP35 family ATP-binding protein, at 264,032 to 264,901
_RS01365
Position (kb) 262
263
264 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 261.921 kb on - strand, within MMJJ_RS01355 at 262.135 kb on + strand, within MMJJ_RS01355 at 262.137 kb on + strand at 262.139 kb on + strand, within MMJJ_RS01355 at 262.336 kb on + strand, within MMJJ_RS01355 at 262.357 kb on + strand at 262.589 kb on - strand, within MMJJ_RS01355 at 262.589 kb on - strand, within MMJJ_RS01355 at 262.590 kb on + strand, within MMJJ_RS01355 at 262.771 kb on + strand at 262.777 kb on - strand at 262.914 kb on + strand at 263.104 kb on - strand, within MMJJ_RS01360 at 263.518 kb on - strand, within MMJJ_RS01360 at 263.723 kb on - strand, within MMJJ_RS01360 at 263.723 kb on - strand, within MMJJ_RS01360 at 263.724 kb on + strand, within MMJJ_RS01360 at 263.801 kb on + strand, within MMJJ_RS01360 at 263.948 kb on - strand at 263.950 kb on - strand at 263.950 kb on - strand at 263.951 kb on + strand at 263.978 kb on - strand at 263.978 kb on - strand at 263.978 kb on - strand at 263.979 kb on + strand at 263.979 kb on + strand at 263.981 kb on - strand at 263.981 kb on - strand at 263.987 kb on + strand at 263.993 kb on - strand at 263.993 kb on - strand at 263.994 kb on + strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.996 kb on + strand at 263.996 kb on + strand at 263.996 kb on + strand at 263.997 kb on - strand at 263.997 kb on - strand at 263.997 kb on - strand at 263.997 kb on - strand at 263.998 kb on + strand at 263.998 kb on + strand at 263.998 kb on + strand at 264.004 kb on - strand at 264.005 kb on + strand at 264.009 kb on + strand at 264.012 kb on - strand at 264.012 kb on - strand at 264.061 kb on - strand at 264.136 kb on - strand, within MMJJ_RS01365 at 264.152 kb on - strand, within MMJJ_RS01365 at 264.255 kb on - strand, within MMJJ_RS01365 at 264.336 kb on - strand, within MMJJ_RS01365 at 264.338 kb on - strand, within MMJJ_RS01365 at 264.338 kb on - strand, within MMJJ_RS01365 at 264.384 kb on + strand, within MMJJ_RS01365 at 264.412 kb on - strand, within MMJJ_RS01365 at 264.414 kb on - strand, within MMJJ_RS01365 at 264.489 kb on - strand, within MMJJ_RS01365 at 264.520 kb on - strand, within MMJJ_RS01365 at 264.591 kb on - strand, within MMJJ_RS01365 at 264.618 kb on - strand, within MMJJ_RS01365 at 264.655 kb on - strand, within MMJJ_RS01365 at 264.681 kb on - strand, within MMJJ_RS01365 at 264.709 kb on - strand, within MMJJ_RS01365 at 264.750 kb on - strand, within MMJJ_RS01365 at 264.827 kb on + strand at 264.838 kb on - strand at 264.899 kb on - strand at 264.899 kb on - strand at 264.904 kb on - strand at 264.938 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 261,921 - MMJJ_RS01355 0.29 -1.3 262,135 + MMJJ_RS01355 0.45 -2.0 262,137 + -1.8 262,139 + MMJJ_RS01355 0.46 -0.9 262,336 + MMJJ_RS01355 0.61 -2.8 262,357 + -1.6 262,589 - MMJJ_RS01355 0.81 -2.5 262,589 - MMJJ_RS01355 0.81 -1.3 262,590 + MMJJ_RS01355 0.81 -1.8 262,771 + -2.0 262,777 - -1.2 262,914 + -2.0 263,104 - MMJJ_RS01360 0.24 -0.5 263,518 - MMJJ_RS01360 0.62 -0.6 263,723 - MMJJ_RS01360 0.80 -1.3 263,723 - MMJJ_RS01360 0.80 -2.9 263,724 + MMJJ_RS01360 0.80 -2.3 263,801 + MMJJ_RS01360 0.87 -1.6 263,948 - -0.2 263,950 - +1.2 263,950 - +0.3 263,951 + -0.4 263,978 - -0.3 263,978 - -1.5 263,978 - -1.1 263,979 + -1.8 263,979 + +0.1 263,981 - -0.5 263,981 - -0.2 263,987 + -0.5 263,993 - +1.1 263,993 - -0.2 263,994 + -0.4 263,995 - -0.8 263,995 - -0.3 263,995 - +1.0 263,995 - +0.6 263,996 + -0.5 263,996 + +0.1 263,996 + -0.0 263,997 - -0.4 263,997 - +0.2 263,997 - -0.2 263,997 - +0.0 263,998 + +0.3 263,998 + +0.7 263,998 + +0.3 264,004 - -0.9 264,005 + +0.6 264,009 + -0.6 264,012 - +0.5 264,012 - -1.6 264,061 - -0.6 264,136 - MMJJ_RS01365 0.12 +0.2 264,152 - MMJJ_RS01365 0.14 +0.3 264,255 - MMJJ_RS01365 0.26 -0.3 264,336 - MMJJ_RS01365 0.35 -0.5 264,338 - MMJJ_RS01365 0.35 -0.1 264,338 - MMJJ_RS01365 0.35 -1.1 264,384 + MMJJ_RS01365 0.40 -0.8 264,412 - MMJJ_RS01365 0.44 -0.5 264,414 - MMJJ_RS01365 0.44 -1.1 264,489 - MMJJ_RS01365 0.53 +1.6 264,520 - MMJJ_RS01365 0.56 +0.3 264,591 - MMJJ_RS01365 0.64 +0.6 264,618 - MMJJ_RS01365 0.67 -0.3 264,655 - MMJJ_RS01365 0.72 -0.9 264,681 - MMJJ_RS01365 0.75 -1.9 264,709 - MMJJ_RS01365 0.78 -1.7 264,750 - MMJJ_RS01365 0.83 +0.2 264,827 + +0.5 264,838 - -0.6 264,899 - -0.0 264,899 - -0.8 264,904 - +0.0 264,938 + +0.1
Or see this region's nucleotide sequence