Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01350 and MMJJ_RS01355 are separated by 12 nucleotides MMJJ_RS01355 and MMJJ_RS01360 are separated by 4 nucleotides
MMJJ_RS01350: MMJJ_RS01350 - sodium:proton antiporter, at 260,285 to 261,538
_RS01350
MMJJ_RS01355: MMJJ_RS01355 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 261,551 to 262,834
_RS01355
MMJJ_RS01360: MMJJ_RS01360 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 262,839 to 263,939
_RS01360
Position (kb) 261
262
263 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 260.578 kb on - strand, within MMJJ_RS01350 at 260.601 kb on - strand, within MMJJ_RS01350 at 260.616 kb on - strand, within MMJJ_RS01350 at 260.617 kb on + strand, within MMJJ_RS01350 at 260.618 kb on - strand, within MMJJ_RS01350 at 260.619 kb on + strand, within MMJJ_RS01350 at 260.619 kb on + strand, within MMJJ_RS01350 at 260.619 kb on + strand at 260.692 kb on - strand, within MMJJ_RS01350 at 260.692 kb on - strand, within MMJJ_RS01350 at 260.693 kb on + strand, within MMJJ_RS01350 at 260.776 kb on - strand, within MMJJ_RS01350 at 260.849 kb on + strand, within MMJJ_RS01350 at 260.933 kb on - strand, within MMJJ_RS01350 at 260.947 kb on + strand, within MMJJ_RS01350 at 260.971 kb on + strand at 260.971 kb on + strand at 261.026 kb on - strand, within MMJJ_RS01350 at 261.238 kb on + strand, within MMJJ_RS01350 at 261.255 kb on - strand, within MMJJ_RS01350 at 261.256 kb on + strand, within MMJJ_RS01350 at 261.369 kb on + strand, within MMJJ_RS01350 at 261.380 kb on + strand, within MMJJ_RS01350 at 261.422 kb on + strand at 261.607 kb on - strand at 261.733 kb on + strand, within MMJJ_RS01355 at 261.733 kb on + strand, within MMJJ_RS01355 at 261.805 kb on - strand, within MMJJ_RS01355 at 261.921 kb on - strand, within MMJJ_RS01355 at 262.135 kb on + strand, within MMJJ_RS01355 at 262.137 kb on + strand at 262.139 kb on + strand, within MMJJ_RS01355 at 262.336 kb on + strand, within MMJJ_RS01355 at 262.357 kb on + strand at 262.589 kb on - strand, within MMJJ_RS01355 at 262.589 kb on - strand, within MMJJ_RS01355 at 262.590 kb on + strand, within MMJJ_RS01355 at 262.771 kb on + strand at 262.777 kb on - strand at 262.914 kb on + strand at 263.104 kb on - strand, within MMJJ_RS01360 at 263.518 kb on - strand, within MMJJ_RS01360 at 263.723 kb on - strand, within MMJJ_RS01360 at 263.723 kb on - strand, within MMJJ_RS01360 at 263.724 kb on + strand, within MMJJ_RS01360 at 263.801 kb on + strand, within MMJJ_RS01360
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 260,578 - MMJJ_RS01350 0.23 -2.7 260,601 - MMJJ_RS01350 0.25 -3.1 260,616 - MMJJ_RS01350 0.26 -1.8 260,617 + MMJJ_RS01350 0.26 -2.2 260,618 - MMJJ_RS01350 0.27 -2.2 260,619 + MMJJ_RS01350 0.27 -2.1 260,619 + MMJJ_RS01350 0.27 -3.2 260,619 + -1.0 260,692 - MMJJ_RS01350 0.32 -3.2 260,692 - MMJJ_RS01350 0.32 -1.8 260,693 + MMJJ_RS01350 0.33 -0.6 260,776 - MMJJ_RS01350 0.39 -2.7 260,849 + MMJJ_RS01350 0.45 -3.0 260,933 - MMJJ_RS01350 0.52 -2.7 260,947 + MMJJ_RS01350 0.53 -2.0 260,971 + -0.6 260,971 + -0.6 261,026 - MMJJ_RS01350 0.59 -2.2 261,238 + MMJJ_RS01350 0.76 -1.3 261,255 - MMJJ_RS01350 0.77 -1.8 261,256 + MMJJ_RS01350 0.77 -3.5 261,369 + MMJJ_RS01350 0.86 -2.3 261,380 + MMJJ_RS01350 0.87 -1.5 261,422 + -2.2 261,607 - -1.8 261,733 + MMJJ_RS01355 0.14 +0.6 261,733 + MMJJ_RS01355 0.14 -1.2 261,805 - MMJJ_RS01355 0.20 -0.6 261,921 - MMJJ_RS01355 0.29 -1.3 262,135 + MMJJ_RS01355 0.45 -2.0 262,137 + -1.8 262,139 + MMJJ_RS01355 0.46 -0.9 262,336 + MMJJ_RS01355 0.61 -2.8 262,357 + -1.6 262,589 - MMJJ_RS01355 0.81 -2.5 262,589 - MMJJ_RS01355 0.81 -1.3 262,590 + MMJJ_RS01355 0.81 -1.8 262,771 + -2.0 262,777 - -1.2 262,914 + -2.0 263,104 - MMJJ_RS01360 0.24 -0.5 263,518 - MMJJ_RS01360 0.62 -0.6 263,723 - MMJJ_RS01360 0.80 -1.3 263,723 - MMJJ_RS01360 0.80 -2.9 263,724 + MMJJ_RS01360 0.80 -2.3 263,801 + MMJJ_RS01360 0.87 -1.6
Or see this region's nucleotide sequence