Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01335 and MMJJ_RS01340 are separated by 216 nucleotides MMJJ_RS01340 and MMJJ_RS01345 are separated by 156 nucleotides MMJJ_RS01345 and MMJJ_RS01350 are separated by 204 nucleotides
MMJJ_RS01335: MMJJ_RS01335 - class III signal peptide-containing protein, at 258,327 to 258,923
_RS01335
MMJJ_RS01340: MMJJ_RS01340 - tRNA-Arg, at 259,140 to 259,213
_RS01340
MMJJ_RS01345: MMJJ_RS01345 - (5-formylfuran-3-yl)methyl phosphate synthase, at 259,370 to 260,080
_RS01345
MMJJ_RS01350: MMJJ_RS01350 - sodium:proton antiporter, at 260,285 to 261,538
_RS01350
Position (kb) 259
260
261 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 258.372 kb on - strand at 258.372 kb on - strand at 258.373 kb on + strand at 258.373 kb on + strand at 258.373 kb on + strand at 258.373 kb on + strand at 258.376 kb on - strand at 258.377 kb on + strand at 258.432 kb on - strand, within MMJJ_RS01335 at 258.433 kb on + strand, within MMJJ_RS01335 at 258.499 kb on - strand, within MMJJ_RS01335 at 258.499 kb on - strand, within MMJJ_RS01335 at 258.499 kb on - strand, within MMJJ_RS01335 at 258.515 kb on - strand, within MMJJ_RS01335 at 258.515 kb on - strand, within MMJJ_RS01335 at 258.524 kb on - strand, within MMJJ_RS01335 at 258.524 kb on - strand, within MMJJ_RS01335 at 258.525 kb on + strand, within MMJJ_RS01335 at 258.558 kb on - strand, within MMJJ_RS01335 at 258.558 kb on - strand, within MMJJ_RS01335 at 258.559 kb on + strand, within MMJJ_RS01335 at 258.578 kb on - strand, within MMJJ_RS01335 at 258.590 kb on + strand, within MMJJ_RS01335 at 258.612 kb on - strand, within MMJJ_RS01335 at 258.613 kb on + strand, within MMJJ_RS01335 at 258.629 kb on + strand, within MMJJ_RS01335 at 258.629 kb on + strand, within MMJJ_RS01335 at 258.633 kb on - strand, within MMJJ_RS01335 at 258.633 kb on - strand, within MMJJ_RS01335 at 258.633 kb on - strand, within MMJJ_RS01335 at 258.636 kb on - strand, within MMJJ_RS01335 at 258.636 kb on - strand, within MMJJ_RS01335 at 258.637 kb on + strand, within MMJJ_RS01335 at 258.637 kb on + strand, within MMJJ_RS01335 at 258.641 kb on - strand, within MMJJ_RS01335 at 258.641 kb on - strand, within MMJJ_RS01335 at 258.641 kb on - strand, within MMJJ_RS01335 at 258.642 kb on + strand, within MMJJ_RS01335 at 258.644 kb on - strand, within MMJJ_RS01335 at 258.644 kb on - strand, within MMJJ_RS01335 at 258.645 kb on + strand, within MMJJ_RS01335 at 258.645 kb on + strand, within MMJJ_RS01335 at 258.650 kb on - strand, within MMJJ_RS01335 at 258.650 kb on - strand, within MMJJ_RS01335 at 258.651 kb on + strand, within MMJJ_RS01335 at 258.654 kb on + strand, within MMJJ_RS01335 at 258.661 kb on - strand, within MMJJ_RS01335 at 258.662 kb on + strand, within MMJJ_RS01335 at 258.671 kb on - strand, within MMJJ_RS01335 at 258.708 kb on + strand, within MMJJ_RS01335 at 258.710 kb on - strand, within MMJJ_RS01335 at 258.711 kb on + strand, within MMJJ_RS01335 at 258.711 kb on + strand, within MMJJ_RS01335 at 258.716 kb on - strand, within MMJJ_RS01335 at 258.717 kb on + strand, within MMJJ_RS01335 at 258.743 kb on - strand, within MMJJ_RS01335 at 258.743 kb on - strand, within MMJJ_RS01335 at 258.756 kb on + strand, within MMJJ_RS01335 at 258.756 kb on + strand, within MMJJ_RS01335 at 258.774 kb on + strand, within MMJJ_RS01335 at 258.843 kb on - strand, within MMJJ_RS01335 at 258.853 kb on - strand, within MMJJ_RS01335 at 258.853 kb on - strand, within MMJJ_RS01335 at 258.853 kb on - strand, within MMJJ_RS01335 at 258.854 kb on + strand, within MMJJ_RS01335 at 258.854 kb on + strand, within MMJJ_RS01335 at 258.868 kb on + strand at 258.879 kb on - strand at 258.882 kb on - strand at 258.888 kb on + strand at 258.898 kb on + strand at 258.898 kb on + strand at 258.898 kb on + strand at 258.945 kb on - strand at 258.945 kb on - strand at 258.945 kb on - strand at 258.945 kb on - strand at 258.945 kb on - strand at 258.946 kb on + strand at 258.946 kb on + strand at 258.946 kb on + strand at 258.946 kb on + strand at 258.946 kb on + strand at 258.946 kb on + strand at 258.946 kb on + strand at 258.946 kb on + strand at 258.946 kb on + strand at 258.946 kb on + strand at 258.947 kb on - strand at 258.947 kb on - strand at 258.947 kb on - strand at 258.947 kb on - strand at 258.947 kb on - strand at 258.948 kb on + strand at 258.948 kb on + strand at 258.948 kb on + strand at 258.948 kb on + strand at 258.948 kb on + strand at 258.948 kb on + strand at 258.948 kb on + strand at 258.951 kb on - strand at 258.951 kb on - strand at 258.951 kb on - strand at 258.951 kb on - strand at 258.951 kb on - strand at 258.951 kb on - strand at 258.951 kb on - strand at 258.952 kb on + strand at 258.952 kb on + strand at 258.952 kb on + strand at 258.952 kb on + strand at 258.952 kb on + strand at 258.952 kb on + strand at 258.956 kb on - strand at 258.956 kb on - strand at 258.956 kb on - strand at 258.956 kb on - strand at 258.956 kb on - strand at 258.956 kb on - strand at 258.956 kb on - strand at 258.956 kb on - strand at 258.957 kb on + strand at 258.957 kb on + strand at 258.957 kb on + strand at 258.958 kb on - strand at 258.959 kb on + strand at 258.959 kb on + strand at 258.964 kb on - strand at 258.964 kb on - strand at 258.964 kb on - strand at 258.965 kb on + strand at 258.965 kb on + strand at 258.966 kb on - strand at 258.966 kb on - strand at 258.966 kb on - strand at 258.974 kb on - strand at 258.974 kb on - strand at 258.975 kb on + strand at 258.975 kb on + strand at 258.975 kb on + strand at 258.975 kb on + strand at 258.982 kb on - strand at 258.987 kb on - strand at 258.988 kb on + strand at 258.988 kb on + strand at 258.993 kb on - strand at 258.993 kb on - strand at 259.001 kb on + strand at 259.001 kb on + strand at 259.003 kb on - strand at 259.003 kb on - strand at 259.004 kb on + strand at 259.006 kb on - strand at 259.007 kb on + strand at 259.007 kb on + strand at 259.012 kb on - strand at 259.012 kb on - strand at 259.016 kb on - strand at 259.016 kb on - strand at 259.017 kb on + strand at 259.019 kb on - strand at 259.019 kb on - strand at 259.019 kb on - strand at 259.020 kb on + strand at 259.020 kb on + strand at 259.020 kb on + strand at 259.020 kb on + strand at 259.025 kb on - strand at 259.033 kb on - strand at 259.130 kb on - strand at 259.132 kb on - strand at 259.132 kb on - strand at 259.137 kb on + strand at 259.197 kb on + strand, within MMJJ_RS01340 at 259.274 kb on - strand at 259.274 kb on - strand at 259.275 kb on + strand at 259.301 kb on + strand at 259.311 kb on + strand at 259.342 kb on - strand at 259.342 kb on - strand at 259.350 kb on - strand at 259.355 kb on - strand at 259.355 kb on - strand at 259.356 kb on + strand at 259.362 kb on - strand at 259.444 kb on - strand, within MMJJ_RS01345 at 259.445 kb on + strand, within MMJJ_RS01345 at 259.466 kb on - strand, within MMJJ_RS01345 at 259.466 kb on - strand at 259.679 kb on - strand, within MMJJ_RS01345 at 259.680 kb on + strand, within MMJJ_RS01345 at 259.831 kb on - strand, within MMJJ_RS01345 at 259.847 kb on - strand, within MMJJ_RS01345 at 259.848 kb on + strand, within MMJJ_RS01345 at 259.848 kb on + strand, within MMJJ_RS01345 at 259.848 kb on + strand, within MMJJ_RS01345 at 259.889 kb on + strand, within MMJJ_RS01345 at 259.889 kb on + strand, within MMJJ_RS01345 at 259.891 kb on + strand, within MMJJ_RS01345 at 260.014 kb on - strand at 260.119 kb on - strand at 260.120 kb on + strand at 260.120 kb on + strand at 260.120 kb on + strand at 260.123 kb on - strand at 260.124 kb on + strand at 260.124 kb on + strand at 260.127 kb on - strand at 260.127 kb on - strand at 260.127 kb on - strand at 260.127 kb on - strand at 260.133 kb on - strand at 260.134 kb on + strand at 260.137 kb on - strand at 260.137 kb on - strand at 260.137 kb on - strand at 260.137 kb on - strand at 260.138 kb on + strand at 260.142 kb on - strand at 260.151 kb on - strand at 260.152 kb on + strand at 260.153 kb on - strand at 260.153 kb on - strand at 260.154 kb on + strand at 260.154 kb on + strand at 260.171 kb on + strand at 260.171 kb on + strand at 260.195 kb on - strand at 260.195 kb on - strand at 260.195 kb on - strand at 260.196 kb on + strand at 260.196 kb on + strand at 260.196 kb on + strand at 260.198 kb on + strand at 260.202 kb on - strand at 260.202 kb on - strand at 260.202 kb on - strand at 260.202 kb on - strand at 260.203 kb on + strand at 260.203 kb on + strand at 260.206 kb on + strand at 260.206 kb on + strand at 260.214 kb on + strand at 260.214 kb on + strand at 260.221 kb on + strand at 260.234 kb on + strand at 260.249 kb on - strand at 260.250 kb on + strand at 260.260 kb on - strand at 260.261 kb on + strand at 260.261 kb on + strand at 260.261 kb on + strand at 260.261 kb on + strand at 260.261 kb on + strand at 260.261 kb on + strand at 260.265 kb on - strand at 260.309 kb on - strand at 260.310 kb on + strand at 260.310 kb on + strand at 260.365 kb on + strand at 260.365 kb on + strand at 260.578 kb on - strand, within MMJJ_RS01350 at 260.601 kb on - strand, within MMJJ_RS01350 at 260.616 kb on - strand, within MMJJ_RS01350 at 260.617 kb on + strand, within MMJJ_RS01350 at 260.618 kb on - strand, within MMJJ_RS01350 at 260.619 kb on + strand, within MMJJ_RS01350 at 260.619 kb on + strand, within MMJJ_RS01350 at 260.619 kb on + strand at 260.692 kb on - strand, within MMJJ_RS01350 at 260.692 kb on - strand, within MMJJ_RS01350 at 260.693 kb on + strand, within MMJJ_RS01350 at 260.776 kb on - strand, within MMJJ_RS01350 at 260.849 kb on + strand, within MMJJ_RS01350 at 260.933 kb on - strand, within MMJJ_RS01350 at 260.947 kb on + strand, within MMJJ_RS01350 at 260.971 kb on + strand at 260.971 kb on + strand at 261.026 kb on - strand, within MMJJ_RS01350
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 258,372 - -0.2 258,372 - -0.0 258,373 + -0.5 258,373 + -0.1 258,373 + +0.3 258,373 + +1.1 258,376 - -0.3 258,377 + +0.5 258,432 - MMJJ_RS01335 0.18 -0.8 258,433 + MMJJ_RS01335 0.18 -0.5 258,499 - MMJJ_RS01335 0.29 -0.2 258,499 - MMJJ_RS01335 0.29 -0.4 258,499 - MMJJ_RS01335 0.29 -0.5 258,515 - MMJJ_RS01335 0.31 +0.3 258,515 - MMJJ_RS01335 0.31 -0.5 258,524 - MMJJ_RS01335 0.33 -0.6 258,524 - MMJJ_RS01335 0.33 +0.1 258,525 + MMJJ_RS01335 0.33 -0.9 258,558 - MMJJ_RS01335 0.39 +0.5 258,558 - MMJJ_RS01335 0.39 -1.2 258,559 + MMJJ_RS01335 0.39 -0.5 258,578 - MMJJ_RS01335 0.42 -0.2 258,590 + MMJJ_RS01335 0.44 +0.3 258,612 - MMJJ_RS01335 0.48 +1.1 258,613 + MMJJ_RS01335 0.48 -0.5 258,629 + MMJJ_RS01335 0.51 -0.7 258,629 + MMJJ_RS01335 0.51 -0.0 258,633 - MMJJ_RS01335 0.51 -0.4 258,633 - MMJJ_RS01335 0.51 +0.8 258,633 - MMJJ_RS01335 0.51 +1.1 258,636 - MMJJ_RS01335 0.52 +0.7 258,636 - MMJJ_RS01335 0.52 -0.6 258,637 + MMJJ_RS01335 0.52 -1.3 258,637 + MMJJ_RS01335 0.52 -1.0 258,641 - MMJJ_RS01335 0.53 +0.8 258,641 - MMJJ_RS01335 0.53 -1.7 258,641 - MMJJ_RS01335 0.53 -0.3 258,642 + MMJJ_RS01335 0.53 +1.2 258,644 - MMJJ_RS01335 0.53 -0.6 258,644 - MMJJ_RS01335 0.53 +0.6 258,645 + MMJJ_RS01335 0.53 +0.3 258,645 + MMJJ_RS01335 0.53 -0.8 258,650 - MMJJ_RS01335 0.54 +0.3 258,650 - MMJJ_RS01335 0.54 -0.3 258,651 + MMJJ_RS01335 0.54 -0.8 258,654 + MMJJ_RS01335 0.55 -0.8 258,661 - MMJJ_RS01335 0.56 +0.7 258,662 + MMJJ_RS01335 0.56 +0.6 258,671 - MMJJ_RS01335 0.58 +0.6 258,708 + MMJJ_RS01335 0.64 -0.6 258,710 - MMJJ_RS01335 0.64 -0.3 258,711 + MMJJ_RS01335 0.64 +0.1 258,711 + MMJJ_RS01335 0.64 -2.7 258,716 - MMJJ_RS01335 0.65 -0.4 258,717 + MMJJ_RS01335 0.65 +0.3 258,743 - MMJJ_RS01335 0.70 -1.2 258,743 - MMJJ_RS01335 0.70 +0.4 258,756 + MMJJ_RS01335 0.72 -1.3 258,756 + MMJJ_RS01335 0.72 +0.6 258,774 + MMJJ_RS01335 0.75 +2.0 258,843 - MMJJ_RS01335 0.86 +0.2 258,853 - MMJJ_RS01335 0.88 +0.7 258,853 - MMJJ_RS01335 0.88 -0.4 258,853 - MMJJ_RS01335 0.88 +0.4 258,854 + MMJJ_RS01335 0.88 +2.2 258,854 + MMJJ_RS01335 0.88 +0.1 258,868 + -1.6 258,879 - -1.1 258,882 - +0.5 258,888 + +0.0 258,898 + +0.3 258,898 + +0.4 258,898 + +1.2 258,945 - +0.6 258,945 - -1.6 258,945 - -0.0 258,945 - +0.4 258,945 - -0.2 258,946 + -1.1 258,946 + +0.8 258,946 + -0.5 258,946 + +0.4 258,946 + +0.7 258,946 + +0.6 258,946 + +0.1 258,946 + +0.4 258,946 + +0.2 258,946 + -0.0 258,947 - -1.3 258,947 - -0.1 258,947 - -0.1 258,947 - +0.4 258,947 - -0.1 258,948 + -0.4 258,948 + +0.7 258,948 + -0.4 258,948 + -0.2 258,948 + -3.0 258,948 + +0.8 258,948 + +0.6 258,951 - -0.1 258,951 - +0.5 258,951 - -1.8 258,951 - -0.2 258,951 - -0.2 258,951 - -0.1 258,951 - +1.0 258,952 + +0.2 258,952 + +0.7 258,952 + +0.6 258,952 + +0.1 258,952 + -1.3 258,952 + -1.6 258,956 - +2.7 258,956 - +0.1 258,956 - -0.3 258,956 - +0.3 258,956 - -0.2 258,956 - -0.7 258,956 - +1.0 258,956 - -0.1 258,957 + -1.0 258,957 + -0.3 258,957 + +0.0 258,958 - +0.9 258,959 + -0.4 258,959 + +0.8 258,964 - -2.6 258,964 - -0.6 258,964 - +1.1 258,965 + +0.3 258,965 + +0.2 258,966 - +1.4 258,966 - +1.1 258,966 - +0.9 258,974 - +0.5 258,974 - -1.7 258,975 + +0.2 258,975 + -1.1 258,975 + -0.3 258,975 + +0.3 258,982 - +0.3 258,987 - -1.0 258,988 + +0.4 258,988 + -0.6 258,993 - +0.1 258,993 - +0.7 259,001 + +0.7 259,001 + +0.1 259,003 - -0.1 259,003 - -0.1 259,004 + -0.4 259,006 - +1.9 259,007 + +0.5 259,007 + -1.2 259,012 - +0.5 259,012 - +0.7 259,016 - +0.6 259,016 - +0.6 259,017 + -0.2 259,019 - +1.9 259,019 - +0.1 259,019 - -0.2 259,020 + -0.9 259,020 + -0.3 259,020 + +0.1 259,020 + -0.4 259,025 - +0.0 259,033 - -1.0 259,130 - +0.5 259,132 - -0.5 259,132 - +0.3 259,137 + +0.2 259,197 + MMJJ_RS01340 0.77 -1.3 259,274 - -1.6 259,274 - -0.0 259,275 + -1.0 259,301 + +1.2 259,311 + +0.4 259,342 - -0.4 259,342 - -0.4 259,350 - -1.5 259,355 - -0.1 259,355 - +0.0 259,356 + +0.3 259,362 - -0.6 259,444 - MMJJ_RS01345 0.10 -1.0 259,445 + MMJJ_RS01345 0.11 -2.2 259,466 - MMJJ_RS01345 0.14 -1.8 259,466 - -1.0 259,679 - MMJJ_RS01345 0.43 -0.7 259,680 + MMJJ_RS01345 0.44 -3.5 259,831 - MMJJ_RS01345 0.65 -3.0 259,847 - MMJJ_RS01345 0.67 -2.6 259,848 + MMJJ_RS01345 0.67 -2.7 259,848 + MMJJ_RS01345 0.67 -2.3 259,848 + MMJJ_RS01345 0.67 -2.8 259,889 + MMJJ_RS01345 0.73 -4.3 259,889 + MMJJ_RS01345 0.73 -2.2 259,891 + MMJJ_RS01345 0.73 -2.0 260,014 - -3.2 260,119 - -0.6 260,120 + -1.6 260,120 + -1.9 260,120 + -3.5 260,123 - -0.1 260,124 + -1.7 260,124 + -3.3 260,127 - +0.5 260,127 - +0.1 260,127 - +0.5 260,127 - +0.0 260,133 - -0.6 260,134 + -3.3 260,137 - +0.2 260,137 - -0.4 260,137 - -1.2 260,137 - +0.4 260,138 + -0.8 260,142 - +0.2 260,151 - -0.5 260,152 + +0.7 260,153 - +0.2 260,153 - -0.3 260,154 + -0.0 260,154 + -0.4 260,171 + -1.0 260,171 + -0.1 260,195 - +1.0 260,195 - -0.6 260,195 - -2.2 260,196 + -0.7 260,196 + +0.4 260,196 + +0.1 260,198 + -1.1 260,202 - -0.0 260,202 - -2.2 260,202 - -0.6 260,202 - -1.6 260,203 + -1.3 260,203 + -0.4 260,206 + -2.3 260,206 + -2.5 260,214 + -0.3 260,214 + -2.5 260,221 + -0.6 260,234 + -2.5 260,249 - -1.3 260,250 + +0.2 260,260 - -2.2 260,261 + +0.8 260,261 + -1.5 260,261 + -1.0 260,261 + -1.7 260,261 + -0.1 260,261 + -0.1 260,265 - -0.4 260,309 - -0.8 260,310 + -2.8 260,310 + -2.2 260,365 + -2.8 260,365 + -0.3 260,578 - MMJJ_RS01350 0.23 -2.7 260,601 - MMJJ_RS01350 0.25 -3.1 260,616 - MMJJ_RS01350 0.26 -1.8 260,617 + MMJJ_RS01350 0.26 -2.2 260,618 - MMJJ_RS01350 0.27 -2.2 260,619 + MMJJ_RS01350 0.27 -2.1 260,619 + MMJJ_RS01350 0.27 -3.2 260,619 + -1.0 260,692 - MMJJ_RS01350 0.32 -3.2 260,692 - MMJJ_RS01350 0.32 -1.8 260,693 + MMJJ_RS01350 0.33 -0.6 260,776 - MMJJ_RS01350 0.39 -2.7 260,849 + MMJJ_RS01350 0.45 -3.0 260,933 - MMJJ_RS01350 0.52 -2.7 260,947 + MMJJ_RS01350 0.53 -2.0 260,971 + -0.6 260,971 + -0.6 261,026 - MMJJ_RS01350 0.59 -2.2
Or see this region's nucleotide sequence