Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00640 and MMJJ_RS00645 are separated by 172 nucleotides MMJJ_RS00645 and MMJJ_RS00650 are separated by 52 nucleotides
MMJJ_RS00640: MMJJ_RS00640 - glycine betaine ABC transporter substrate-binding protein, at 123,819 to 124,706
_RS00640
MMJJ_RS00645: MMJJ_RS00645 - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 124,879 to 126,282
_RS00645
MMJJ_RS00650: MMJJ_RS00650 - cation:proton antiporter, at 126,335 to 127,501
_RS00650
Position (kb)
124
125
126
127 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 123.956 kb on - strand, within MMJJ_RS00640 at 124.003 kb on + strand, within MMJJ_RS00640 at 124.015 kb on - strand, within MMJJ_RS00640 at 124.038 kb on + strand, within MMJJ_RS00640 at 124.072 kb on - strand, within MMJJ_RS00640 at 124.073 kb on + strand, within MMJJ_RS00640 at 124.110 kb on - strand, within MMJJ_RS00640 at 124.150 kb on - strand, within MMJJ_RS00640 at 124.150 kb on - strand, within MMJJ_RS00640 at 124.151 kb on + strand, within MMJJ_RS00640 at 124.151 kb on + strand, within MMJJ_RS00640 at 124.188 kb on + strand, within MMJJ_RS00640 at 124.191 kb on - strand, within MMJJ_RS00640 at 124.191 kb on - strand, within MMJJ_RS00640 at 124.191 kb on - strand, within MMJJ_RS00640 at 124.192 kb on + strand, within MMJJ_RS00640 at 124.192 kb on + strand, within MMJJ_RS00640 at 124.192 kb on + strand, within MMJJ_RS00640 at 124.197 kb on - strand, within MMJJ_RS00640 at 124.197 kb on - strand, within MMJJ_RS00640 at 124.197 kb on - strand, within MMJJ_RS00640 at 124.197 kb on - strand, within MMJJ_RS00640 at 124.198 kb on + strand, within MMJJ_RS00640 at 124.198 kb on + strand, within MMJJ_RS00640 at 124.198 kb on + strand, within MMJJ_RS00640 at 124.211 kb on + strand, within MMJJ_RS00640 at 124.229 kb on + strand, within MMJJ_RS00640 at 124.247 kb on + strand, within MMJJ_RS00640 at 124.289 kb on - strand, within MMJJ_RS00640 at 124.296 kb on + strand, within MMJJ_RS00640 at 124.296 kb on + strand, within MMJJ_RS00640 at 124.320 kb on - strand, within MMJJ_RS00640 at 124.320 kb on - strand, within MMJJ_RS00640 at 124.328 kb on + strand, within MMJJ_RS00640 at 124.391 kb on - strand, within MMJJ_RS00640 at 124.412 kb on + strand, within MMJJ_RS00640 at 124.415 kb on - strand, within MMJJ_RS00640 at 124.483 kb on - strand, within MMJJ_RS00640 at 124.484 kb on + strand, within MMJJ_RS00640 at 124.484 kb on + strand, within MMJJ_RS00640 at 124.504 kb on - strand, within MMJJ_RS00640 at 124.504 kb on - strand, within MMJJ_RS00640 at 124.505 kb on + strand, within MMJJ_RS00640 at 124.505 kb on + strand, within MMJJ_RS00640 at 124.505 kb on + strand, within MMJJ_RS00640 at 124.506 kb on - strand, within MMJJ_RS00640 at 124.506 kb on - strand, within MMJJ_RS00640 at 124.507 kb on + strand, within MMJJ_RS00640 at 124.511 kb on - strand, within MMJJ_RS00640 at 124.511 kb on - strand, within MMJJ_RS00640 at 124.512 kb on + strand at 124.551 kb on - strand, within MMJJ_RS00640 at 124.577 kb on + strand, within MMJJ_RS00640 at 124.605 kb on - strand, within MMJJ_RS00640 at 124.605 kb on - strand, within MMJJ_RS00640 at 124.605 kb on - strand, within MMJJ_RS00640 at 124.605 kb on - strand, within MMJJ_RS00640 at 124.605 kb on - strand, within MMJJ_RS00640 at 124.606 kb on + strand, within MMJJ_RS00640 at 124.611 kb on - strand, within MMJJ_RS00640 at 124.612 kb on + strand, within MMJJ_RS00640 at 124.612 kb on + strand, within MMJJ_RS00640 at 124.715 kb on - strand at 124.717 kb on - strand at 124.717 kb on - strand at 124.759 kb on + strand at 124.791 kb on - strand at 124.793 kb on - strand at 124.793 kb on - strand at 124.793 kb on - strand at 124.794 kb on + strand at 124.809 kb on + strand at 124.820 kb on + strand at 124.820 kb on + strand at 124.823 kb on + strand at 124.924 kb on + strand at 124.963 kb on - strand at 125.013 kb on + strand at 125.035 kb on - strand, within MMJJ_RS00645 at 125.125 kb on + strand, within MMJJ_RS00645 at 125.284 kb on + strand, within MMJJ_RS00645 at 125.408 kb on - strand, within MMJJ_RS00645 at 125.409 kb on + strand, within MMJJ_RS00645 at 125.444 kb on + strand, within MMJJ_RS00645 at 125.451 kb on - strand, within MMJJ_RS00645 at 125.598 kb on - strand, within MMJJ_RS00645 at 125.598 kb on - strand, within MMJJ_RS00645 at 125.599 kb on + strand, within MMJJ_RS00645 at 125.614 kb on + strand, within MMJJ_RS00645 at 125.659 kb on + strand, within MMJJ_RS00645 at 125.661 kb on - strand, within MMJJ_RS00645 at 125.662 kb on + strand, within MMJJ_RS00645 at 125.662 kb on + strand, within MMJJ_RS00645 at 125.663 kb on - strand, within MMJJ_RS00645 at 125.667 kb on + strand, within MMJJ_RS00645 at 125.667 kb on + strand, within MMJJ_RS00645 at 125.669 kb on - strand, within MMJJ_RS00645 at 125.670 kb on + strand, within MMJJ_RS00645 at 125.670 kb on + strand, within MMJJ_RS00645 at 125.670 kb on + strand, within MMJJ_RS00645 at 125.714 kb on + strand, within MMJJ_RS00645 at 125.733 kb on + strand, within MMJJ_RS00645 at 125.804 kb on - strand, within MMJJ_RS00645 at 125.805 kb on + strand, within MMJJ_RS00645 at 125.826 kb on + strand, within MMJJ_RS00645 at 125.860 kb on + strand, within MMJJ_RS00645 at 125.860 kb on + strand, within MMJJ_RS00645 at 125.884 kb on - strand, within MMJJ_RS00645 at 125.885 kb on + strand, within MMJJ_RS00645 at 125.901 kb on + strand, within MMJJ_RS00645 at 125.903 kb on - strand, within MMJJ_RS00645 at 125.903 kb on - strand, within MMJJ_RS00645 at 125.904 kb on + strand, within MMJJ_RS00645 at 125.963 kb on - strand, within MMJJ_RS00645 at 125.981 kb on - strand, within MMJJ_RS00645 at 126.046 kb on - strand, within MMJJ_RS00645 at 126.076 kb on + strand, within MMJJ_RS00645 at 126.197 kb on - strand at 126.281 kb on + strand at 126.281 kb on + strand at 126.331 kb on - strand at 126.332 kb on + strand at 126.332 kb on + strand at 126.333 kb on - strand at 126.337 kb on + strand at 126.353 kb on - strand at 126.370 kb on - strand at 126.370 kb on - strand at 126.370 kb on - strand at 126.370 kb on - strand at 126.371 kb on + strand at 126.386 kb on + strand at 126.393 kb on + strand at 126.524 kb on + strand, within MMJJ_RS00650 at 126.542 kb on - strand, within MMJJ_RS00650 at 126.574 kb on - strand, within MMJJ_RS00650 at 126.587 kb on - strand, within MMJJ_RS00650 at 126.588 kb on + strand, within MMJJ_RS00650 at 126.593 kb on - strand, within MMJJ_RS00650 at 126.594 kb on + strand, within MMJJ_RS00650 at 126.615 kb on + strand, within MMJJ_RS00650 at 126.641 kb on - strand, within MMJJ_RS00650 at 126.663 kb on - strand, within MMJJ_RS00650 at 126.663 kb on - strand, within MMJJ_RS00650 at 126.663 kb on - strand, within MMJJ_RS00650 at 126.663 kb on - strand, within MMJJ_RS00650 at 126.663 kb on - strand, within MMJJ_RS00650 at 126.684 kb on + strand, within MMJJ_RS00650 at 126.835 kb on - strand, within MMJJ_RS00650 at 126.836 kb on + strand, within MMJJ_RS00650 at 127.002 kb on + strand, within MMJJ_RS00650 at 127.115 kb on - strand, within MMJJ_RS00650 at 127.130 kb on + strand, within MMJJ_RS00650 at 127.141 kb on - strand, within MMJJ_RS00650 at 127.155 kb on - strand, within MMJJ_RS00650 at 127.155 kb on - strand, within MMJJ_RS00650 at 127.156 kb on + strand, within MMJJ_RS00650 at 127.169 kb on + strand, within MMJJ_RS00650 at 127.215 kb on + strand, within MMJJ_RS00650 at 127.215 kb on + strand, within MMJJ_RS00650 at 127.247 kb on - strand, within MMJJ_RS00650 at 127.248 kb on + strand, within MMJJ_RS00650 at 127.248 kb on + strand, within MMJJ_RS00650 at 127.248 kb on + strand, within MMJJ_RS00650 at 127.248 kb on + strand, within MMJJ_RS00650 at 127.254 kb on + strand, within MMJJ_RS00650
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 123,956 - MMJJ_RS00640 0.15 +0.7 124,003 + MMJJ_RS00640 0.21 +0.8 124,015 - MMJJ_RS00640 0.22 +0.1 124,038 + MMJJ_RS00640 0.25 -0.2 124,072 - MMJJ_RS00640 0.28 -0.4 124,073 + MMJJ_RS00640 0.29 +0.2 124,110 - MMJJ_RS00640 0.33 +0.9 124,150 - MMJJ_RS00640 0.37 +0.8 124,150 - MMJJ_RS00640 0.37 +0.3 124,151 + MMJJ_RS00640 0.37 +0.1 124,151 + MMJJ_RS00640 0.37 -1.1 124,188 + MMJJ_RS00640 0.42 -0.6 124,191 - MMJJ_RS00640 0.42 +0.2 124,191 - MMJJ_RS00640 0.42 +1.7 124,191 - MMJJ_RS00640 0.42 +0.0 124,192 + MMJJ_RS00640 0.42 +0.2 124,192 + MMJJ_RS00640 0.42 -0.1 124,192 + MMJJ_RS00640 0.42 -0.7 124,197 - MMJJ_RS00640 0.43 -0.5 124,197 - MMJJ_RS00640 0.43 -0.3 124,197 - MMJJ_RS00640 0.43 -0.3 124,197 - MMJJ_RS00640 0.43 +0.5 124,198 + MMJJ_RS00640 0.43 -0.4 124,198 + MMJJ_RS00640 0.43 +0.2 124,198 + MMJJ_RS00640 0.43 +0.1 124,211 + MMJJ_RS00640 0.44 -0.3 124,229 + MMJJ_RS00640 0.46 +0.1 124,247 + MMJJ_RS00640 0.48 -0.6 124,289 - MMJJ_RS00640 0.53 +0.2 124,296 + MMJJ_RS00640 0.54 -0.1 124,296 + MMJJ_RS00640 0.54 -0.7 124,320 - MMJJ_RS00640 0.56 +0.3 124,320 - MMJJ_RS00640 0.56 -0.3 124,328 + MMJJ_RS00640 0.57 +0.5 124,391 - MMJJ_RS00640 0.64 +0.2 124,412 + MMJJ_RS00640 0.67 -0.1 124,415 - MMJJ_RS00640 0.67 +0.4 124,483 - MMJJ_RS00640 0.75 -0.5 124,484 + MMJJ_RS00640 0.75 +0.5 124,484 + MMJJ_RS00640 0.75 +0.5 124,504 - MMJJ_RS00640 0.77 +0.4 124,504 - MMJJ_RS00640 0.77 +0.3 124,505 + MMJJ_RS00640 0.77 +0.4 124,505 + MMJJ_RS00640 0.77 +1.3 124,505 + MMJJ_RS00640 0.77 -0.6 124,506 - MMJJ_RS00640 0.77 -0.6 124,506 - MMJJ_RS00640 0.77 +0.4 124,507 + MMJJ_RS00640 0.77 +0.2 124,511 - MMJJ_RS00640 0.78 -0.5 124,511 - MMJJ_RS00640 0.78 -0.7 124,512 + +1.4 124,551 - MMJJ_RS00640 0.82 -0.2 124,577 + MMJJ_RS00640 0.85 +0.6 124,605 - MMJJ_RS00640 0.89 +0.2 124,605 - MMJJ_RS00640 0.89 -2.2 124,605 - MMJJ_RS00640 0.89 -1.0 124,605 - MMJJ_RS00640 0.89 -0.3 124,605 - MMJJ_RS00640 0.89 +0.6 124,606 + MMJJ_RS00640 0.89 +0.6 124,611 - MMJJ_RS00640 0.89 +0.1 124,612 + MMJJ_RS00640 0.89 -1.6 124,612 + MMJJ_RS00640 0.89 -0.4 124,715 - -1.8 124,717 - -0.0 124,717 - +0.1 124,759 + -0.9 124,791 - -0.0 124,793 - -2.6 124,793 - -2.2 124,793 - -1.2 124,794 + -0.8 124,809 + +0.9 124,820 + +0.7 124,820 + -0.9 124,823 + -0.9 124,924 + -1.8 124,963 - -1.5 125,013 + -4.3 125,035 - MMJJ_RS00645 0.11 -3.5 125,125 + MMJJ_RS00645 0.18 -3.0 125,284 + MMJJ_RS00645 0.29 -2.3 125,408 - MMJJ_RS00645 0.38 -2.2 125,409 + MMJJ_RS00645 0.38 -2.6 125,444 + MMJJ_RS00645 0.40 -4.2 125,451 - MMJJ_RS00645 0.41 -3.0 125,598 - MMJJ_RS00645 0.51 -0.6 125,598 - MMJJ_RS00645 0.51 -3.8 125,599 + MMJJ_RS00645 0.51 -2.8 125,614 + MMJJ_RS00645 0.52 -3.0 125,659 + MMJJ_RS00645 0.56 -1.8 125,661 - MMJJ_RS00645 0.56 -4.1 125,662 + MMJJ_RS00645 0.56 -3.3 125,662 + MMJJ_RS00645 0.56 -1.3 125,663 - MMJJ_RS00645 0.56 -2.8 125,667 + MMJJ_RS00645 0.56 -2.6 125,667 + MMJJ_RS00645 0.56 -4.3 125,669 - MMJJ_RS00645 0.56 -4.9 125,670 + MMJJ_RS00645 0.56 -4.0 125,670 + MMJJ_RS00645 0.56 -2.0 125,670 + MMJJ_RS00645 0.56 -2.4 125,714 + MMJJ_RS00645 0.59 -2.8 125,733 + MMJJ_RS00645 0.61 -4.1 125,804 - MMJJ_RS00645 0.66 -3.3 125,805 + MMJJ_RS00645 0.66 -2.7 125,826 + MMJJ_RS00645 0.67 -3.2 125,860 + MMJJ_RS00645 0.70 -2.8 125,860 + MMJJ_RS00645 0.70 -2.8 125,884 - MMJJ_RS00645 0.72 -4.3 125,885 + MMJJ_RS00645 0.72 -1.4 125,901 + MMJJ_RS00645 0.73 -2.3 125,903 - MMJJ_RS00645 0.73 -2.8 125,903 - MMJJ_RS00645 0.73 -2.0 125,904 + MMJJ_RS00645 0.73 -1.9 125,963 - MMJJ_RS00645 0.77 -3.2 125,981 - MMJJ_RS00645 0.78 -3.0 126,046 - MMJJ_RS00645 0.83 -2.7 126,076 + MMJJ_RS00645 0.85 -2.2 126,197 - -4.0 126,281 + -1.8 126,281 + +0.4 126,331 - +0.5 126,332 + +0.5 126,332 + +0.3 126,333 - -0.2 126,337 + +1.3 126,353 - +0.3 126,370 - -0.1 126,370 - +0.2 126,370 - +0.6 126,370 - -0.7 126,371 + -3.5 126,386 + +0.4 126,393 + -0.3 126,524 + MMJJ_RS00650 0.16 +1.4 126,542 - MMJJ_RS00650 0.18 -0.3 126,574 - MMJJ_RS00650 0.20 -0.2 126,587 - MMJJ_RS00650 0.22 -1.0 126,588 + MMJJ_RS00650 0.22 +0.2 126,593 - MMJJ_RS00650 0.22 +0.2 126,594 + MMJJ_RS00650 0.22 +0.4 126,615 + MMJJ_RS00650 0.24 +0.1 126,641 - MMJJ_RS00650 0.26 -0.3 126,663 - MMJJ_RS00650 0.28 -1.3 126,663 - MMJJ_RS00650 0.28 -0.0 126,663 - MMJJ_RS00650 0.28 -0.9 126,663 - MMJJ_RS00650 0.28 -0.2 126,663 - MMJJ_RS00650 0.28 +0.2 126,684 + MMJJ_RS00650 0.30 +0.2 126,835 - MMJJ_RS00650 0.43 -1.0 126,836 + MMJJ_RS00650 0.43 -0.5 127,002 + MMJJ_RS00650 0.57 +0.4 127,115 - MMJJ_RS00650 0.67 -0.4 127,130 + MMJJ_RS00650 0.68 -1.0 127,141 - MMJJ_RS00650 0.69 +0.5 127,155 - MMJJ_RS00650 0.70 +0.5 127,155 - MMJJ_RS00650 0.70 -0.2 127,156 + MMJJ_RS00650 0.70 +0.5 127,169 + MMJJ_RS00650 0.71 +0.1 127,215 + MMJJ_RS00650 0.75 -0.2 127,215 + MMJJ_RS00650 0.75 +0.9 127,247 - MMJJ_RS00650 0.78 -0.7 127,248 + MMJJ_RS00650 0.78 +0.0 127,248 + MMJJ_RS00650 0.78 +0.5 127,248 + MMJJ_RS00650 0.78 +0.6 127,248 + MMJJ_RS00650 0.78 +0.5 127,254 + MMJJ_RS00650 0.79 -1.4
Or see this region's nucleotide sequence