Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00570 and MMJJ_RS00575 are separated by 88 nucleotides MMJJ_RS00575 and MMJJ_RS00580 are separated by 22 nucleotides MMJJ_RS00580 and MMJJ_RS00585 are separated by 9 nucleotides
MMJJ_RS00570: MMJJ_RS00570 - ribonuclease P, at 106,921 to 107,313
_RS00570
MMJJ_RS00575: MMJJ_RS00575 - carboxylating nicotinate-nucleotide diphosphorylase, at 107,402 to 108,241
_RS00575
MMJJ_RS00580: MMJJ_RS00580 - 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit CofG, at 108,264 to 109,322
_RS00580
MMJJ_RS00585: MMJJ_RS00585 - S-layer protein, at 109,332 to 110,978
_RS00585
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 107.316 kb on - strand at 107.316 kb on - strand at 107.329 kb on - strand at 107.329 kb on - strand at 107.330 kb on + strand at 107.330 kb on + strand at 107.331 kb on - strand at 107.331 kb on - strand at 107.332 kb on + strand at 107.332 kb on + strand at 107.337 kb on + strand at 107.347 kb on - strand at 107.359 kb on - strand at 107.359 kb on - strand at 107.359 kb on - strand at 107.368 kb on - strand at 107.369 kb on + strand at 107.369 kb on + strand at 107.382 kb on + strand at 107.383 kb on - strand at 107.454 kb on - strand at 108.022 kb on + strand at 108.054 kb on - strand, within MMJJ_RS00575 at 108.066 kb on - strand, within MMJJ_RS00575 at 108.142 kb on - strand, within MMJJ_RS00575 at 108.179 kb on + strand at 108.239 kb on - strand at 108.264 kb on + strand at 108.606 kb on - strand, within MMJJ_RS00580 at 109.052 kb on + strand, within MMJJ_RS00580 at 109.065 kb on + strand, within MMJJ_RS00580 at 109.270 kb on + strand at 109.316 kb on - strand at 109.317 kb on + strand at 109.321 kb on + strand at 109.347 kb on - strand at 109.371 kb on + strand at 109.431 kb on - strand at 109.476 kb on + strand at 109.648 kb on + strand, within MMJJ_RS00585 at 109.656 kb on - strand, within MMJJ_RS00585 at 109.657 kb on + strand, within MMJJ_RS00585 at 109.675 kb on + strand, within MMJJ_RS00585 at 109.793 kb on - strand, within MMJJ_RS00585 at 109.800 kb on - strand, within MMJJ_RS00585 at 109.863 kb on + strand, within MMJJ_RS00585 at 109.874 kb on - strand, within MMJJ_RS00585 at 109.874 kb on - strand, within MMJJ_RS00585 at 109.874 kb on - strand, within MMJJ_RS00585 at 109.874 kb on - strand, within MMJJ_RS00585 at 109.875 kb on + strand, within MMJJ_RS00585 at 109.875 kb on + strand, within MMJJ_RS00585 at 109.875 kb on + strand, within MMJJ_RS00585 at 109.875 kb on + strand, within MMJJ_RS00585 at 109.876 kb on - strand, within MMJJ_RS00585 at 109.881 kb on - strand, within MMJJ_RS00585 at 109.886 kb on - strand, within MMJJ_RS00585 at 109.887 kb on + strand, within MMJJ_RS00585 at 109.889 kb on - strand, within MMJJ_RS00585 at 109.893 kb on + strand, within MMJJ_RS00585 at 109.910 kb on + strand, within MMJJ_RS00585 at 109.928 kb on - strand, within MMJJ_RS00585 at 109.929 kb on + strand, within MMJJ_RS00585 at 109.931 kb on + strand, within MMJJ_RS00585 at 109.971 kb on - strand, within MMJJ_RS00585 at 110.073 kb on - strand, within MMJJ_RS00585 at 110.074 kb on + strand, within MMJJ_RS00585 at 110.084 kb on - strand, within MMJJ_RS00585 at 110.149 kb on + strand, within MMJJ_RS00585 at 110.149 kb on + strand, within MMJJ_RS00585 at 110.149 kb on + strand, within MMJJ_RS00585 at 110.149 kb on + strand, within MMJJ_RS00585 at 110.153 kb on - strand, within MMJJ_RS00585 at 110.189 kb on - strand, within MMJJ_RS00585 at 110.189 kb on - strand, within MMJJ_RS00585 at 110.190 kb on + strand, within MMJJ_RS00585 at 110.190 kb on + strand, within MMJJ_RS00585 at 110.190 kb on + strand, within MMJJ_RS00585 at 110.192 kb on + strand, within MMJJ_RS00585 at 110.192 kb on + strand, within MMJJ_RS00585 at 110.207 kb on - strand, within MMJJ_RS00585 at 110.208 kb on + strand, within MMJJ_RS00585 at 110.211 kb on - strand, within MMJJ_RS00585 at 110.264 kb on - strand, within MMJJ_RS00585 at 110.265 kb on + strand, within MMJJ_RS00585 at 110.270 kb on - strand, within MMJJ_RS00585
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 107,316 - -2.3 107,316 - -2.3 107,329 - -0.8 107,329 - +0.6 107,330 + +0.7 107,330 + +0.7 107,331 - -0.9 107,331 - -0.3 107,332 + -0.9 107,332 + -3.5 107,337 + -0.1 107,347 - +0.4 107,359 - +2.3 107,359 - -1.1 107,359 - -3.7 107,368 - +0.1 107,369 + -2.0 107,369 + -1.0 107,382 + -0.6 107,383 - -1.3 107,454 - -3.9 108,022 + -1.6 108,054 - MMJJ_RS00575 0.78 +2.0 108,066 - MMJJ_RS00575 0.79 -3.0 108,142 - MMJJ_RS00575 0.88 -1.2 108,179 + -1.3 108,239 - -1.6 108,264 + -1.6 108,606 - MMJJ_RS00580 0.32 -1.3 109,052 + MMJJ_RS00580 0.74 -2.6 109,065 + MMJJ_RS00580 0.76 -3.7 109,270 + -0.8 109,316 - +0.4 109,317 + +0.2 109,321 + -0.1 109,347 - -2.5 109,371 + -0.1 109,431 - +0.5 109,476 + -0.8 109,648 + MMJJ_RS00585 0.19 -0.9 109,656 - MMJJ_RS00585 0.20 -1.4 109,657 + MMJJ_RS00585 0.20 +0.1 109,675 + MMJJ_RS00585 0.21 +0.3 109,793 - MMJJ_RS00585 0.28 -0.3 109,800 - MMJJ_RS00585 0.28 -0.3 109,863 + MMJJ_RS00585 0.32 +0.5 109,874 - MMJJ_RS00585 0.33 -0.4 109,874 - MMJJ_RS00585 0.33 +0.5 109,874 - MMJJ_RS00585 0.33 -0.2 109,874 - MMJJ_RS00585 0.33 +0.1 109,875 + MMJJ_RS00585 0.33 -0.6 109,875 + MMJJ_RS00585 0.33 -1.2 109,875 + MMJJ_RS00585 0.33 -0.2 109,875 + MMJJ_RS00585 0.33 +0.9 109,876 - MMJJ_RS00585 0.33 -3.1 109,881 - MMJJ_RS00585 0.33 -0.5 109,886 - MMJJ_RS00585 0.34 +0.7 109,887 + MMJJ_RS00585 0.34 -0.8 109,889 - MMJJ_RS00585 0.34 +0.2 109,893 + MMJJ_RS00585 0.34 -0.3 109,910 + MMJJ_RS00585 0.35 +0.9 109,928 - MMJJ_RS00585 0.36 -0.3 109,929 + MMJJ_RS00585 0.36 -0.6 109,931 + MMJJ_RS00585 0.36 +0.6 109,971 - MMJJ_RS00585 0.39 +1.0 110,073 - MMJJ_RS00585 0.45 -0.6 110,074 + MMJJ_RS00585 0.45 -0.5 110,084 - MMJJ_RS00585 0.46 +0.1 110,149 + MMJJ_RS00585 0.50 -0.1 110,149 + MMJJ_RS00585 0.50 -0.5 110,149 + MMJJ_RS00585 0.50 +0.3 110,149 + MMJJ_RS00585 0.50 -0.1 110,153 - MMJJ_RS00585 0.50 -0.3 110,189 - MMJJ_RS00585 0.52 +0.5 110,189 - MMJJ_RS00585 0.52 +0.6 110,190 + MMJJ_RS00585 0.52 +0.3 110,190 + MMJJ_RS00585 0.52 -0.4 110,190 + MMJJ_RS00585 0.52 -0.1 110,192 + MMJJ_RS00585 0.52 -0.9 110,192 + MMJJ_RS00585 0.52 +0.7 110,207 - MMJJ_RS00585 0.53 +2.5 110,208 + MMJJ_RS00585 0.53 -2.3 110,211 - MMJJ_RS00585 0.53 -0.9 110,264 - MMJJ_RS00585 0.57 +0.5 110,265 + MMJJ_RS00585 0.57 +0.4 110,270 - MMJJ_RS00585 0.57 +0.5
Or see this region's nucleotide sequence