Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00550 and MMJJ_RS00555 are separated by 174 nucleotides MMJJ_RS00555 and MMJJ_RS00560 are separated by 124 nucleotides MMJJ_RS00560 and MMJJ_RS00565 are separated by 62 nucleotides
MMJJ_RS00550: MMJJ_RS00550 - AIR synthase-related protein, at 102,500 to 103,381
_RS00550
MMJJ_RS00555: MMJJ_RS00555 - hypothetical protein, at 103,556 to 103,897
_RS00555
MMJJ_RS00560: MMJJ_RS00560 - homoisocitrate dehydrogenase, at 104,022 to 105,041
_RS00560
MMJJ_RS00565: MMJJ_RS00565 - serine--tRNA ligase, at 105,104 to 106,648
_RS00565
Position (kb) 104
105
106 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 103.031 kb on - strand, within MMJJ_RS00550 at 103.032 kb on + strand, within MMJJ_RS00550 at 103.046 kb on + strand, within MMJJ_RS00550 at 103.076 kb on - strand, within MMJJ_RS00550 at 103.077 kb on + strand, within MMJJ_RS00550 at 103.128 kb on - strand, within MMJJ_RS00550 at 103.128 kb on - strand, within MMJJ_RS00550 at 103.165 kb on - strand, within MMJJ_RS00550 at 103.165 kb on - strand, within MMJJ_RS00550 at 103.165 kb on - strand, within MMJJ_RS00550 at 103.166 kb on + strand, within MMJJ_RS00550 at 103.166 kb on + strand, within MMJJ_RS00550 at 103.194 kb on + strand, within MMJJ_RS00550 at 103.244 kb on - strand, within MMJJ_RS00550 at 103.245 kb on + strand, within MMJJ_RS00550 at 103.318 kb on - strand at 103.339 kb on - strand at 103.339 kb on - strand at 103.339 kb on - strand at 103.339 kb on - strand at 103.339 kb on - strand at 103.340 kb on + strand at 103.340 kb on + strand at 103.340 kb on + strand at 103.340 kb on + strand at 103.346 kb on - strand at 103.346 kb on - strand at 103.347 kb on + strand at 103.354 kb on + strand at 103.399 kb on - strand at 103.400 kb on + strand at 103.416 kb on + strand at 103.418 kb on + strand at 103.422 kb on - strand at 103.433 kb on + strand at 103.448 kb on - strand at 103.452 kb on - strand at 103.453 kb on + strand at 103.453 kb on + strand at 103.454 kb on - strand at 103.454 kb on - strand at 103.454 kb on - strand at 103.455 kb on + strand at 103.455 kb on + strand at 103.460 kb on - strand at 103.460 kb on - strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.464 kb on - strand at 103.464 kb on - strand at 103.464 kb on - strand at 103.464 kb on - strand at 103.464 kb on - strand at 103.464 kb on - strand at 103.465 kb on + strand at 103.465 kb on + strand at 103.474 kb on + strand at 103.481 kb on + strand at 103.486 kb on - strand at 103.490 kb on - strand at 103.504 kb on + strand at 103.506 kb on + strand at 103.506 kb on + strand at 103.521 kb on - strand at 103.523 kb on - strand at 103.524 kb on + strand at 103.535 kb on + strand at 103.554 kb on - strand at 103.560 kb on - strand at 103.590 kb on - strand at 103.648 kb on - strand, within MMJJ_RS00555 at 103.663 kb on - strand, within MMJJ_RS00555 at 103.663 kb on - strand, within MMJJ_RS00555 at 103.663 kb on - strand, within MMJJ_RS00555 at 103.664 kb on + strand, within MMJJ_RS00555 at 103.667 kb on - strand, within MMJJ_RS00555 at 103.667 kb on - strand, within MMJJ_RS00555 at 103.667 kb on - strand, within MMJJ_RS00555 at 103.667 kb on - strand, within MMJJ_RS00555 at 103.667 kb on - strand, within MMJJ_RS00555 at 103.668 kb on + strand, within MMJJ_RS00555 at 103.668 kb on + strand, within MMJJ_RS00555 at 103.668 kb on + strand, within MMJJ_RS00555 at 103.668 kb on + strand, within MMJJ_RS00555 at 103.668 kb on + strand, within MMJJ_RS00555 at 103.668 kb on + strand, within MMJJ_RS00555 at 103.670 kb on + strand, within MMJJ_RS00555 at 103.690 kb on - strand, within MMJJ_RS00555 at 103.715 kb on + strand, within MMJJ_RS00555 at 103.715 kb on + strand, within MMJJ_RS00555 at 103.762 kb on + strand, within MMJJ_RS00555 at 103.777 kb on - strand, within MMJJ_RS00555 at 103.793 kb on - strand, within MMJJ_RS00555 at 103.794 kb on + strand, within MMJJ_RS00555 at 103.813 kb on - strand, within MMJJ_RS00555 at 103.813 kb on - strand, within MMJJ_RS00555 at 103.813 kb on - strand, within MMJJ_RS00555 at 103.886 kb on - strand at 103.895 kb on - strand at 103.896 kb on + strand at 103.896 kb on + strand at 103.899 kb on - strand at 103.900 kb on + strand at 103.902 kb on - strand at 103.903 kb on + strand at 103.907 kb on - strand at 103.908 kb on + strand at 103.911 kb on - strand at 103.912 kb on + strand at 103.912 kb on + strand at 103.934 kb on - strand at 103.934 kb on - strand at 103.935 kb on + strand at 103.938 kb on + strand at 103.938 kb on + strand at 103.938 kb on + strand at 103.944 kb on - strand at 103.945 kb on + strand at 103.970 kb on - strand at 103.982 kb on + strand at 104.101 kb on - strand at 104.273 kb on - strand, within MMJJ_RS00560 at 104.274 kb on + strand, within MMJJ_RS00560 at 104.334 kb on - strand, within MMJJ_RS00560 at 104.363 kb on + strand, within MMJJ_RS00560 at 104.542 kb on - strand, within MMJJ_RS00560 at 104.543 kb on + strand at 104.543 kb on + strand, within MMJJ_RS00560 at 104.633 kb on + strand, within MMJJ_RS00560 at 104.713 kb on - strand, within MMJJ_RS00560 at 104.891 kb on + strand, within MMJJ_RS00560 at 105.045 kb on - strand at 105.045 kb on - strand at 105.045 kb on - strand at 105.045 kb on - strand at 105.070 kb on + strand at 105.078 kb on - strand at 105.404 kb on - strand, within MMJJ_RS00565 at 105.869 kb on - strand at 105.869 kb on - strand at 105.912 kb on + strand, within MMJJ_RS00565
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 103,031 - MMJJ_RS00550 0.60 -0.9 103,032 + MMJJ_RS00550 0.60 -4.4 103,046 + MMJJ_RS00550 0.62 +0.1 103,076 - MMJJ_RS00550 0.65 -1.9 103,077 + MMJJ_RS00550 0.65 -1.0 103,128 - MMJJ_RS00550 0.71 -2.6 103,128 - MMJJ_RS00550 0.71 -0.4 103,165 - MMJJ_RS00550 0.75 +1.4 103,165 - MMJJ_RS00550 0.75 -0.0 103,165 - MMJJ_RS00550 0.75 -0.2 103,166 + MMJJ_RS00550 0.76 +0.8 103,166 + MMJJ_RS00550 0.76 -3.0 103,194 + MMJJ_RS00550 0.79 -2.6 103,244 - MMJJ_RS00550 0.84 -0.8 103,245 + MMJJ_RS00550 0.84 +0.1 103,318 - -1.0 103,339 - -3.3 103,339 - -4.0 103,339 - +0.0 103,339 - -0.3 103,339 - -3.7 103,340 + +0.2 103,340 + +0.4 103,340 + -1.4 103,340 + -3.2 103,346 - -1.4 103,346 - -2.3 103,347 + -0.7 103,354 + -1.5 103,399 - +0.9 103,400 + +0.1 103,416 + -0.4 103,418 + -0.8 103,422 - +0.4 103,433 + +0.2 103,448 - -0.7 103,452 - +0.0 103,453 + +0.8 103,453 + +0.5 103,454 - -0.0 103,454 - +1.0 103,454 - -0.4 103,455 + +0.9 103,455 + +0.5 103,460 - +0.2 103,460 - +0.7 103,461 + -0.0 103,461 + -0.6 103,461 + +0.5 103,461 + +0.1 103,461 + -0.8 103,461 + +0.2 103,461 + +1.8 103,464 - +0.1 103,464 - -0.2 103,464 - +0.7 103,464 - +0.8 103,464 - -0.3 103,464 - +0.7 103,465 + +1.6 103,465 + -0.6 103,474 + -0.3 103,481 + -0.5 103,486 - +0.2 103,490 - -0.6 103,504 + -1.2 103,506 + -1.3 103,506 + -0.3 103,521 - -0.9 103,523 - -0.5 103,524 + -0.1 103,535 + +0.4 103,554 - +0.1 103,560 - +0.1 103,590 - -0.3 103,648 - MMJJ_RS00555 0.27 -0.2 103,663 - MMJJ_RS00555 0.31 +0.7 103,663 - MMJJ_RS00555 0.31 +0.5 103,663 - MMJJ_RS00555 0.31 +0.7 103,664 + MMJJ_RS00555 0.32 -0.3 103,667 - MMJJ_RS00555 0.32 +0.3 103,667 - MMJJ_RS00555 0.32 +0.7 103,667 - MMJJ_RS00555 0.32 +0.3 103,667 - MMJJ_RS00555 0.32 +0.8 103,667 - MMJJ_RS00555 0.32 +0.0 103,668 + MMJJ_RS00555 0.33 +0.7 103,668 + MMJJ_RS00555 0.33 +0.7 103,668 + MMJJ_RS00555 0.33 +0.2 103,668 + MMJJ_RS00555 0.33 -1.0 103,668 + MMJJ_RS00555 0.33 +1.3 103,668 + MMJJ_RS00555 0.33 +0.0 103,670 + MMJJ_RS00555 0.33 -1.8 103,690 - MMJJ_RS00555 0.39 -1.7 103,715 + MMJJ_RS00555 0.46 +0.4 103,715 + MMJJ_RS00555 0.46 -0.2 103,762 + MMJJ_RS00555 0.60 +0.4 103,777 - MMJJ_RS00555 0.65 -0.6 103,793 - MMJJ_RS00555 0.69 +0.0 103,794 + MMJJ_RS00555 0.70 +0.2 103,813 - MMJJ_RS00555 0.75 +0.1 103,813 - MMJJ_RS00555 0.75 +0.4 103,813 - MMJJ_RS00555 0.75 -0.6 103,886 - -2.0 103,895 - +0.5 103,896 + -0.5 103,896 + +0.2 103,899 - -0.4 103,900 + +0.6 103,902 - +0.3 103,903 + -0.1 103,907 - -0.3 103,908 + +1.0 103,911 - +0.6 103,912 + +0.2 103,912 + +1.3 103,934 - +0.0 103,934 - -2.3 103,935 + -0.2 103,938 + +0.1 103,938 + -0.6 103,938 + +0.6 103,944 - +0.1 103,945 + -1.4 103,970 - -1.3 103,982 + -1.9 104,101 - -2.3 104,273 - MMJJ_RS00560 0.25 -0.7 104,274 + MMJJ_RS00560 0.25 -1.8 104,334 - MMJJ_RS00560 0.31 -2.4 104,363 + MMJJ_RS00560 0.33 -2.8 104,542 - MMJJ_RS00560 0.51 -3.0 104,543 + -1.6 104,543 + MMJJ_RS00560 0.51 -2.8 104,633 + MMJJ_RS00560 0.60 -3.4 104,713 - MMJJ_RS00560 0.68 -3.5 104,891 + MMJJ_RS00560 0.85 -2.4 105,045 - -2.1 105,045 - -1.1 105,045 - -0.6 105,045 - -2.9 105,070 + -2.0 105,078 - -3.0 105,404 - MMJJ_RS00565 0.19 -3.2 105,869 - -0.6 105,869 - -1.6 105,912 + MMJJ_RS00565 0.52 -1.3
Or see this region's nucleotide sequence