Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00210 and MMJJ_RS00215 are separated by 12 nucleotides MMJJ_RS00215 and MMJJ_RS00220 are separated by 57 nucleotides MMJJ_RS00220 and MMJJ_RS00225 are separated by 281 nucleotides
MMJJ_RS00210: MMJJ_RS00210 - DUF530 family protein, at 39,577 to 41,007
_RS00210
MMJJ_RS00215: MMJJ_RS00215 - CCA tRNA nucleotidyltransferase, at 41,020 to 42,354
_RS00215
MMJJ_RS00220: MMJJ_RS00220 - UPF0058 family protein, at 42,412 to 42,768
_RS00220
MMJJ_RS00225: MMJJ_RS00225 - ATP phosphoribosyltransferase, at 43,050 to 43,916
_RS00225
Position (kb) 41
42
43 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 40.416 kb on - strand at 40.450 kb on - strand at 40.490 kb on + strand, within MMJJ_RS00210 at 40.601 kb on - strand, within MMJJ_RS00210 at 40.759 kb on + strand, within MMJJ_RS00210 at 40.769 kb on - strand, within MMJJ_RS00210 at 41.021 kb on - strand at 41.041 kb on + strand at 41.041 kb on + strand at 41.045 kb on + strand at 41.045 kb on + strand at 41.072 kb on + strand at 41.076 kb on + strand at 41.238 kb on + strand, within MMJJ_RS00215 at 41.502 kb on + strand, within MMJJ_RS00215 at 41.570 kb on + strand, within MMJJ_RS00215 at 41.679 kb on + strand, within MMJJ_RS00215 at 41.875 kb on - strand, within MMJJ_RS00215 at 42.033 kb on + strand, within MMJJ_RS00215 at 42.330 kb on + strand at 42.372 kb on + strand at 42.390 kb on + strand at 42.390 kb on + strand at 42.393 kb on - strand at 42.393 kb on - strand at 42.394 kb on + strand at 42.398 kb on - strand at 42.414 kb on + strand at 42.485 kb on - strand, within MMJJ_RS00220 at 42.505 kb on - strand, within MMJJ_RS00220 at 42.505 kb on - strand, within MMJJ_RS00220 at 42.505 kb on - strand, within MMJJ_RS00220 at 42.505 kb on - strand, within MMJJ_RS00220 at 42.505 kb on - strand, within MMJJ_RS00220 at 42.509 kb on - strand, within MMJJ_RS00220 at 42.509 kb on - strand, within MMJJ_RS00220 at 42.513 kb on - strand, within MMJJ_RS00220 at 42.662 kb on + strand, within MMJJ_RS00220 at 42.782 kb on - strand at 42.800 kb on + strand at 42.804 kb on - strand at 42.817 kb on - strand at 42.818 kb on + strand at 42.818 kb on + strand at 42.819 kb on - strand at 42.819 kb on - strand at 42.819 kb on - strand at 42.820 kb on + strand at 42.820 kb on + strand at 42.820 kb on + strand at 42.820 kb on + strand at 42.822 kb on + strand at 42.822 kb on + strand at 42.823 kb on - strand at 42.823 kb on - strand at 42.824 kb on + strand at 42.824 kb on + strand at 42.844 kb on - strand at 42.844 kb on - strand at 42.863 kb on - strand at 42.868 kb on + strand at 42.884 kb on + strand at 42.901 kb on + strand at 42.907 kb on - strand at 42.907 kb on - strand at 42.912 kb on - strand at 42.912 kb on - strand at 42.912 kb on - strand at 42.912 kb on - strand at 42.913 kb on + strand at 42.913 kb on + strand at 42.913 kb on + strand at 42.914 kb on - strand at 42.915 kb on + strand at 42.915 kb on + strand at 42.915 kb on + strand at 42.915 kb on + strand at 42.915 kb on + strand at 42.916 kb on - strand at 42.916 kb on - strand at 42.916 kb on - strand at 42.917 kb on + strand at 42.917 kb on + strand at 42.917 kb on + strand at 42.917 kb on + strand at 42.919 kb on - strand at 42.919 kb on - strand at 42.919 kb on - strand at 42.919 kb on - strand at 42.920 kb on + strand at 42.920 kb on + strand at 42.920 kb on + strand at 42.920 kb on + strand at 42.920 kb on + strand at 42.920 kb on + strand at 42.927 kb on - strand at 42.927 kb on - strand at 42.928 kb on + strand at 42.928 kb on + strand at 42.928 kb on + strand at 42.928 kb on + strand at 42.929 kb on - strand at 42.929 kb on - strand at 42.929 kb on - strand at 42.929 kb on - strand at 42.930 kb on + strand at 42.930 kb on + strand at 42.930 kb on + strand at 42.930 kb on + strand at 42.930 kb on + strand at 42.935 kb on - strand at 42.936 kb on + strand at 42.936 kb on + strand at 42.939 kb on - strand at 42.939 kb on - strand at 42.940 kb on + strand at 42.940 kb on + strand at 42.962 kb on - strand at 42.963 kb on + strand at 43.007 kb on + strand at 43.136 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 40,416 - -0.6 40,450 - -1.0 40,490 + MMJJ_RS00210 0.64 -1.6 40,601 - MMJJ_RS00210 0.72 -2.6 40,759 + MMJJ_RS00210 0.83 -2.2 40,769 - MMJJ_RS00210 0.83 -2.4 41,021 - -2.9 41,041 + -0.5 41,041 + +1.3 41,045 + -0.6 41,045 + -0.7 41,072 + -0.7 41,076 + -1.3 41,238 + MMJJ_RS00215 0.16 -2.2 41,502 + MMJJ_RS00215 0.36 -1.6 41,570 + MMJJ_RS00215 0.41 -0.6 41,679 + MMJJ_RS00215 0.49 -1.6 41,875 - MMJJ_RS00215 0.64 -0.3 42,033 + MMJJ_RS00215 0.76 -3.1 42,330 + -2.0 42,372 + -2.2 42,390 + -3.5 42,390 + -2.4 42,393 - -2.6 42,393 - -1.0 42,394 + -2.4 42,398 - -0.1 42,414 + +1.0 42,485 - MMJJ_RS00220 0.20 -0.3 42,505 - MMJJ_RS00220 0.26 -2.9 42,505 - MMJJ_RS00220 0.26 +0.0 42,505 - MMJJ_RS00220 0.26 -3.4 42,505 - MMJJ_RS00220 0.26 +0.2 42,505 - MMJJ_RS00220 0.26 -0.5 42,509 - MMJJ_RS00220 0.27 +0.3 42,509 - MMJJ_RS00220 0.27 -2.3 42,513 - MMJJ_RS00220 0.28 +0.9 42,662 + MMJJ_RS00220 0.70 -0.6 42,782 - -4.2 42,800 + -0.4 42,804 - -0.6 42,817 - -2.2 42,818 + -3.2 42,818 + -4.4 42,819 - -1.0 42,819 - -1.2 42,819 - -2.4 42,820 + -0.7 42,820 + -2.6 42,820 + -2.5 42,820 + -4.7 42,822 + -1.6 42,822 + +0.2 42,823 - -0.6 42,823 - -0.4 42,824 + +1.4 42,824 + +0.1 42,844 - +0.1 42,844 - -1.0 42,863 - -0.1 42,868 + +0.1 42,884 + -0.6 42,901 + -0.2 42,907 - -2.9 42,907 - -0.6 42,912 - +0.2 42,912 - +0.5 42,912 - -0.4 42,912 - -0.7 42,913 + -0.9 42,913 + -1.3 42,913 + +0.3 42,914 - -0.7 42,915 + +0.0 42,915 + -0.4 42,915 + +0.5 42,915 + +0.7 42,915 + -0.9 42,916 - -0.8 42,916 - -0.5 42,916 - +0.0 42,917 + +0.8 42,917 + +0.5 42,917 + -0.4 42,917 + +1.3 42,919 - +0.9 42,919 - +0.0 42,919 - -0.1 42,919 - +0.2 42,920 + -0.6 42,920 + -0.2 42,920 + +0.4 42,920 + +0.5 42,920 + +0.1 42,920 + +0.7 42,927 - +0.8 42,927 - -0.9 42,928 + +0.6 42,928 + +0.2 42,928 + -1.9 42,928 + -2.1 42,929 - +0.4 42,929 - -0.7 42,929 - +0.6 42,929 - +0.9 42,930 + -0.1 42,930 + -0.7 42,930 + +0.2 42,930 + +0.3 42,930 + +0.3 42,935 - -0.2 42,936 + -1.2 42,936 + -0.8 42,939 - +0.8 42,939 - +0.6 42,940 + -0.2 42,940 + +1.3 42,962 - -2.4 42,963 + -2.9 43,007 + -2.2 43,136 + -1.3
Or see this region's nucleotide sequence