Experiment: PYE with Benzethonium chloride 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02097 and CCNA_02098 are separated by 88 nucleotides CCNA_02098 and CCNA_02099 overlap by 4 nucleotides CCNA_02099 and CCNA_02100 are separated by 118 nucleotides CCNA_02100 and CCNA_02101 are separated by 116 nucleotides
CCNA_02097: CCNA_02097 - periplasmic glucan glucosyltransferase, at 2,245,508 to 2,247,499
_02097
CCNA_02098: CCNA_02098 - molybdopterin-guanine dinucleotide biosynthesis protein A, at 2,247,588 to 2,248,484
_02098
CCNA_02099: CCNA_02099 - Na+ driven multidrug efflux pump, at 2,248,481 to 2,249,896
_02099
CCNA_02100: CCNA_02100 - hypothetical protein, at 2,250,015 to 2,250,494
_02100
CCNA_02101: CCNA_02101 - glyoxalase family protein, at 2,250,611 to 2,250,997
_02101
Position (kb)
2248
2249
2250 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2247.494 kb on + strand at 2247.502 kb on - strand at 2247.512 kb on + strand at 2247.526 kb on + strand at 2247.532 kb on + strand at 2247.537 kb on + strand at 2247.699 kb on + strand, within CCNA_02098 at 2247.707 kb on - strand, within CCNA_02098 at 2247.745 kb on + strand, within CCNA_02098 at 2247.753 kb on - strand, within CCNA_02098 at 2247.762 kb on + strand, within CCNA_02098 at 2247.853 kb on - strand, within CCNA_02098 at 2247.853 kb on - strand, within CCNA_02098 at 2247.867 kb on + strand, within CCNA_02098 at 2247.882 kb on + strand, within CCNA_02098 at 2247.905 kb on + strand, within CCNA_02098 at 2247.945 kb on - strand, within CCNA_02098 at 2247.976 kb on - strand, within CCNA_02098 at 2247.998 kb on - strand, within CCNA_02098 at 2248.026 kb on + strand, within CCNA_02098 at 2248.039 kb on - strand, within CCNA_02098 at 2248.122 kb on + strand, within CCNA_02098 at 2248.196 kb on + strand, within CCNA_02098 at 2248.204 kb on - strand, within CCNA_02098 at 2248.206 kb on + strand, within CCNA_02098 at 2248.206 kb on + strand, within CCNA_02098 at 2248.219 kb on - strand, within CCNA_02098 at 2248.262 kb on - strand, within CCNA_02098 at 2248.264 kb on + strand, within CCNA_02098 at 2248.272 kb on - strand, within CCNA_02098 at 2248.272 kb on - strand, within CCNA_02098 at 2248.286 kb on + strand, within CCNA_02098 at 2248.286 kb on + strand, within CCNA_02098 at 2248.286 kb on + strand, within CCNA_02098 at 2248.286 kb on + strand, within CCNA_02098 at 2248.294 kb on - strand, within CCNA_02098 at 2248.299 kb on - strand, within CCNA_02098 at 2248.301 kb on + strand, within CCNA_02098 at 2248.301 kb on + strand, within CCNA_02098 at 2248.301 kb on + strand, within CCNA_02098 at 2248.301 kb on + strand, within CCNA_02098 at 2248.301 kb on + strand at 2248.301 kb on + strand, within CCNA_02098 at 2248.301 kb on + strand, within CCNA_02098 at 2248.301 kb on + strand, within CCNA_02098 at 2248.301 kb on + strand, within CCNA_02098 at 2248.309 kb on - strand, within CCNA_02098 at 2248.309 kb on - strand, within CCNA_02098 at 2248.309 kb on - strand, within CCNA_02098 at 2248.309 kb on - strand, within CCNA_02098 at 2248.309 kb on - strand, within CCNA_02098 at 2248.309 kb on - strand, within CCNA_02098 at 2248.315 kb on - strand, within CCNA_02098 at 2248.329 kb on + strand, within CCNA_02098 at 2248.341 kb on + strand, within CCNA_02098 at 2248.369 kb on + strand, within CCNA_02098 at 2248.387 kb on - strand, within CCNA_02098 at 2248.416 kb on + strand at 2248.498 kb on - strand at 2248.592 kb on + strand at 2248.592 kb on + strand at 2248.600 kb on - strand at 2248.605 kb on + strand at 2248.638 kb on + strand, within CCNA_02099 at 2248.638 kb on + strand, within CCNA_02099 at 2248.638 kb on + strand, within CCNA_02099 at 2248.646 kb on - strand, within CCNA_02099 at 2248.646 kb on - strand, within CCNA_02099 at 2248.656 kb on - strand, within CCNA_02099 at 2248.656 kb on - strand, within CCNA_02099 at 2248.666 kb on - strand, within CCNA_02099 at 2248.666 kb on - strand, within CCNA_02099 at 2248.688 kb on - strand, within CCNA_02099 at 2248.729 kb on - strand, within CCNA_02099 at 2248.783 kb on - strand, within CCNA_02099 at 2248.813 kb on + strand, within CCNA_02099 at 2248.816 kb on - strand, within CCNA_02099 at 2248.816 kb on - strand, within CCNA_02099 at 2248.818 kb on + strand, within CCNA_02099 at 2248.826 kb on - strand, within CCNA_02099 at 2248.833 kb on + strand, within CCNA_02099 at 2248.833 kb on + strand, within CCNA_02099 at 2248.841 kb on - strand, within CCNA_02099 at 2248.841 kb on - strand, within CCNA_02099 at 2248.884 kb on - strand, within CCNA_02099 at 2248.901 kb on + strand, within CCNA_02099 at 2248.909 kb on - strand, within CCNA_02099 at 2248.934 kb on - strand, within CCNA_02099 at 2248.934 kb on - strand, within CCNA_02099 at 2248.986 kb on - strand, within CCNA_02099 at 2248.993 kb on - strand, within CCNA_02099 at 2249.026 kb on - strand, within CCNA_02099 at 2249.039 kb on + strand, within CCNA_02099 at 2249.059 kb on - strand, within CCNA_02099 at 2249.227 kb on - strand, within CCNA_02099 at 2249.227 kb on - strand, within CCNA_02099 at 2249.227 kb on - strand, within CCNA_02099 at 2249.267 kb on + strand, within CCNA_02099 at 2249.329 kb on - strand, within CCNA_02099 at 2249.359 kb on + strand, within CCNA_02099 at 2249.362 kb on - strand, within CCNA_02099 at 2249.367 kb on - strand, within CCNA_02099 at 2249.377 kb on + strand, within CCNA_02099 at 2249.385 kb on - strand, within CCNA_02099 at 2249.390 kb on + strand, within CCNA_02099 at 2249.398 kb on - strand, within CCNA_02099 at 2249.426 kb on - strand, within CCNA_02099 at 2249.428 kb on + strand, within CCNA_02099 at 2249.474 kb on + strand, within CCNA_02099 at 2249.474 kb on + strand, within CCNA_02099 at 2249.489 kb on - strand, within CCNA_02099 at 2249.502 kb on + strand, within CCNA_02099 at 2249.502 kb on + strand, within CCNA_02099 at 2249.510 kb on - strand, within CCNA_02099 at 2249.516 kb on + strand, within CCNA_02099 at 2249.535 kb on + strand, within CCNA_02099 at 2249.567 kb on + strand, within CCNA_02099 at 2249.575 kb on - strand, within CCNA_02099 at 2249.575 kb on - strand, within CCNA_02099 at 2249.575 kb on - strand, within CCNA_02099 at 2249.595 kb on - strand, within CCNA_02099 at 2249.619 kb on + strand, within CCNA_02099 at 2249.619 kb on + strand, within CCNA_02099 at 2249.619 kb on + strand, within CCNA_02099 at 2249.627 kb on - strand, within CCNA_02099 at 2249.638 kb on + strand, within CCNA_02099 at 2249.668 kb on - strand, within CCNA_02099 at 2249.679 kb on + strand, within CCNA_02099 at 2249.741 kb on + strand, within CCNA_02099 at 2249.741 kb on + strand, within CCNA_02099 at 2249.741 kb on + strand, within CCNA_02099 at 2249.749 kb on - strand, within CCNA_02099 at 2249.784 kb on - strand at 2249.801 kb on - strand at 2249.816 kb on + strand at 2249.825 kb on - strand at 2249.880 kb on + strand at 2249.880 kb on + strand at 2249.883 kb on - strand at 2249.883 kb on - strand at 2249.888 kb on - strand at 2249.903 kb on + strand at 2249.955 kb on - strand at 2249.960 kb on - strand at 2250.041 kb on - strand at 2250.048 kb on - strand at 2250.179 kb on - strand, within CCNA_02100 at 2250.187 kb on - strand, within CCNA_02100 at 2250.212 kb on - strand, within CCNA_02100 at 2250.221 kb on - strand, within CCNA_02100 at 2250.231 kb on - strand, within CCNA_02100 at 2250.231 kb on - strand, within CCNA_02100 at 2250.248 kb on + strand, within CCNA_02100 at 2250.272 kb on + strand, within CCNA_02100 at 2250.363 kb on + strand, within CCNA_02100 at 2250.364 kb on + strand, within CCNA_02100 at 2250.382 kb on + strand, within CCNA_02100 at 2250.390 kb on - strand, within CCNA_02100 at 2250.409 kb on + strand, within CCNA_02100 at 2250.453 kb on + strand at 2250.461 kb on - strand at 2250.461 kb on - strand at 2250.530 kb on + strand at 2250.696 kb on - strand, within CCNA_02101 at 2250.708 kb on - strand, within CCNA_02101 at 2250.832 kb on + strand, within CCNA_02101 at 2250.841 kb on - strand, within CCNA_02101
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Benzethonium chloride 0.001 mM remove 2,247,494 + +3.0 2,247,502 - +0.4 2,247,512 + +1.2 2,247,526 + +2.2 2,247,532 + +1.8 2,247,537 + +1.7 2,247,699 + CCNA_02098 0.12 +2.0 2,247,707 - CCNA_02098 0.13 +0.4 2,247,745 + CCNA_02098 0.18 +1.0 2,247,753 - CCNA_02098 0.18 -0.2 2,247,762 + CCNA_02098 0.19 +3.0 2,247,853 - CCNA_02098 0.30 +0.7 2,247,853 - CCNA_02098 0.30 -1.2 2,247,867 + CCNA_02098 0.31 +1.8 2,247,882 + CCNA_02098 0.33 +2.6 2,247,905 + CCNA_02098 0.35 +1.6 2,247,945 - CCNA_02098 0.40 +0.4 2,247,976 - CCNA_02098 0.43 +1.2 2,247,998 - CCNA_02098 0.46 -0.4 2,248,026 + CCNA_02098 0.49 +2.1 2,248,039 - CCNA_02098 0.50 -0.2 2,248,122 + CCNA_02098 0.60 +2.1 2,248,196 + CCNA_02098 0.68 +2.2 2,248,204 - CCNA_02098 0.69 +1.2 2,248,206 + CCNA_02098 0.69 +2.4 2,248,206 + CCNA_02098 0.69 +1.8 2,248,219 - CCNA_02098 0.70 -0.8 2,248,262 - CCNA_02098 0.75 +0.9 2,248,264 + CCNA_02098 0.75 +1.5 2,248,272 - CCNA_02098 0.76 +0.4 2,248,272 - CCNA_02098 0.76 +1.4 2,248,286 + CCNA_02098 0.78 +2.7 2,248,286 + CCNA_02098 0.78 +1.8 2,248,286 + CCNA_02098 0.78 +2.0 2,248,286 + CCNA_02098 0.78 +1.7 2,248,294 - CCNA_02098 0.79 -0.3 2,248,299 - CCNA_02098 0.79 +1.5 2,248,301 + CCNA_02098 0.79 +2.1 2,248,301 + CCNA_02098 0.79 +2.5 2,248,301 + CCNA_02098 0.79 +2.5 2,248,301 + CCNA_02098 0.79 +2.1 2,248,301 + +3.0 2,248,301 + CCNA_02098 0.79 +3.0 2,248,301 + CCNA_02098 0.79 +3.4 2,248,301 + CCNA_02098 0.79 +2.2 2,248,301 + CCNA_02098 0.79 +2.9 2,248,309 - CCNA_02098 0.80 +1.2 2,248,309 - CCNA_02098 0.80 +1.5 2,248,309 - CCNA_02098 0.80 -1.6 2,248,309 - CCNA_02098 0.80 +3.6 2,248,309 - CCNA_02098 0.80 +0.2 2,248,309 - CCNA_02098 0.80 -1.0 2,248,315 - CCNA_02098 0.81 -1.3 2,248,329 + CCNA_02098 0.83 +2.0 2,248,341 + CCNA_02098 0.84 +1.9 2,248,369 + CCNA_02098 0.87 +2.1 2,248,387 - CCNA_02098 0.89 +2.0 2,248,416 + +1.8 2,248,498 - +1.3 2,248,592 + -0.1 2,248,592 + +1.0 2,248,600 - +1.5 2,248,605 + -0.0 2,248,638 + CCNA_02099 0.11 -1.6 2,248,638 + CCNA_02099 0.11 +0.2 2,248,638 + CCNA_02099 0.11 +1.4 2,248,646 - CCNA_02099 0.12 +0.5 2,248,646 - CCNA_02099 0.12 -0.0 2,248,656 - CCNA_02099 0.12 +1.1 2,248,656 - CCNA_02099 0.12 +1.6 2,248,666 - CCNA_02099 0.13 +2.2 2,248,666 - CCNA_02099 0.13 +1.8 2,248,688 - CCNA_02099 0.15 +1.1 2,248,729 - CCNA_02099 0.18 +1.2 2,248,783 - CCNA_02099 0.21 +1.3 2,248,813 + CCNA_02099 0.23 +0.4 2,248,816 - CCNA_02099 0.24 +0.4 2,248,816 - CCNA_02099 0.24 +0.1 2,248,818 + CCNA_02099 0.24 -1.2 2,248,826 - CCNA_02099 0.24 +0.6 2,248,833 + CCNA_02099 0.25 -0.9 2,248,833 + CCNA_02099 0.25 -1.9 2,248,841 - CCNA_02099 0.25 +0.4 2,248,841 - CCNA_02099 0.25 +1.3 2,248,884 - CCNA_02099 0.28 +0.1 2,248,901 + CCNA_02099 0.30 +0.4 2,248,909 - CCNA_02099 0.30 +1.1 2,248,934 - CCNA_02099 0.32 +0.9 2,248,934 - CCNA_02099 0.32 +2.0 2,248,986 - CCNA_02099 0.36 -0.8 2,248,993 - CCNA_02099 0.36 +2.3 2,249,026 - CCNA_02099 0.38 +0.6 2,249,039 + CCNA_02099 0.39 +1.1 2,249,059 - CCNA_02099 0.41 -0.5 2,249,227 - CCNA_02099 0.53 +1.1 2,249,227 - CCNA_02099 0.53 +0.5 2,249,227 - CCNA_02099 0.53 +1.3 2,249,267 + CCNA_02099 0.56 -0.8 2,249,329 - CCNA_02099 0.60 -2.2 2,249,359 + CCNA_02099 0.62 -0.0 2,249,362 - CCNA_02099 0.62 +1.3 2,249,367 - CCNA_02099 0.63 +2.3 2,249,377 + CCNA_02099 0.63 -0.0 2,249,385 - CCNA_02099 0.64 +1.6 2,249,390 + CCNA_02099 0.64 +2.0 2,249,398 - CCNA_02099 0.65 -0.5 2,249,426 - CCNA_02099 0.67 +1.1 2,249,428 + CCNA_02099 0.67 -2.2 2,249,474 + CCNA_02099 0.70 -0.0 2,249,474 + CCNA_02099 0.70 +1.0 2,249,489 - CCNA_02099 0.71 +0.4 2,249,502 + CCNA_02099 0.72 -1.8 2,249,502 + CCNA_02099 0.72 +0.4 2,249,510 - CCNA_02099 0.73 +1.2 2,249,516 + CCNA_02099 0.73 -0.8 2,249,535 + CCNA_02099 0.74 +2.3 2,249,567 + CCNA_02099 0.77 -2.7 2,249,575 - CCNA_02099 0.77 +1.1 2,249,575 - CCNA_02099 0.77 +1.0 2,249,575 - CCNA_02099 0.77 +0.7 2,249,595 - CCNA_02099 0.79 +2.4 2,249,619 + CCNA_02099 0.80 -0.3 2,249,619 + CCNA_02099 0.80 -0.6 2,249,619 + CCNA_02099 0.80 -0.2 2,249,627 - CCNA_02099 0.81 +1.0 2,249,638 + CCNA_02099 0.82 -2.1 2,249,668 - CCNA_02099 0.84 +0.7 2,249,679 + CCNA_02099 0.85 -2.1 2,249,741 + CCNA_02099 0.89 -1.7 2,249,741 + CCNA_02099 0.89 -0.3 2,249,741 + CCNA_02099 0.89 -2.3 2,249,749 - CCNA_02099 0.90 +0.5 2,249,784 - -0.1 2,249,801 - +1.4 2,249,816 + -2.6 2,249,825 - +1.4 2,249,880 + -1.3 2,249,880 + -0.3 2,249,883 - +2.4 2,249,883 - +1.5 2,249,888 - +0.1 2,249,903 + -1.0 2,249,955 - +1.2 2,249,960 - +1.3 2,250,041 - -0.7 2,250,048 - -0.6 2,250,179 - CCNA_02100 0.34 +0.2 2,250,187 - CCNA_02100 0.36 +0.1 2,250,212 - CCNA_02100 0.41 +0.6 2,250,221 - CCNA_02100 0.43 +0.4 2,250,231 - CCNA_02100 0.45 +0.4 2,250,231 - CCNA_02100 0.45 +0.4 2,250,248 + CCNA_02100 0.49 +1.0 2,250,272 + CCNA_02100 0.54 -1.2 2,250,363 + CCNA_02100 0.72 -2.5 2,250,364 + CCNA_02100 0.73 -0.6 2,250,382 + CCNA_02100 0.76 -1.5 2,250,390 - CCNA_02100 0.78 -0.8 2,250,409 + CCNA_02100 0.82 -1.0 2,250,453 + +0.9 2,250,461 - -0.1 2,250,461 - -0.0 2,250,530 + -0.0 2,250,696 - CCNA_02101 0.22 -0.0 2,250,708 - CCNA_02101 0.25 +1.0 2,250,832 + CCNA_02101 0.57 -2.5 2,250,841 - CCNA_02101 0.59 -2.1
Or see this region's nucleotide sequence