Experiment: PYE with Benzethonium chloride 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00514 and CCNA_00515 are separated by 415 nucleotides CCNA_00515 and CCNA_00516 are separated by 25 nucleotides CCNA_00516 and CCNA_00517 are separated by 2 nucleotides
CCNA_00514: CCNA_00514 - pirin, at 528,078 to 528,908
_00514
CCNA_00515: CCNA_00515 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase, at 529,324 to 531,657
_00515
CCNA_00516: CCNA_00516 - integral membrane protein, at 531,683 to 532,432
_00516
CCNA_00517: CCNA_00517 - peptidyl-tRNA hydrolase, at 532,435 to 533,052
_00517
Position (kb) 529
530
531
532 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 528.330 kb on + strand, within CCNA_00514 at 528.337 kb on - strand, within CCNA_00514 at 528.338 kb on - strand, within CCNA_00514 at 528.369 kb on + strand, within CCNA_00514 at 528.405 kb on + strand, within CCNA_00514 at 528.405 kb on + strand, within CCNA_00514 at 528.413 kb on - strand, within CCNA_00514 at 528.424 kb on + strand, within CCNA_00514 at 528.424 kb on + strand, within CCNA_00514 at 528.432 kb on - strand, within CCNA_00514 at 528.432 kb on - strand, within CCNA_00514 at 528.436 kb on + strand, within CCNA_00514 at 528.436 kb on + strand, within CCNA_00514 at 528.621 kb on + strand, within CCNA_00514 at 528.645 kb on - strand, within CCNA_00514 at 528.645 kb on - strand, within CCNA_00514 at 528.662 kb on + strand, within CCNA_00514 at 528.690 kb on + strand, within CCNA_00514 at 528.690 kb on + strand, within CCNA_00514 at 528.698 kb on - strand, within CCNA_00514 at 528.713 kb on - strand, within CCNA_00514 at 528.757 kb on - strand, within CCNA_00514 at 528.777 kb on + strand, within CCNA_00514 at 528.802 kb on - strand, within CCNA_00514 at 528.832 kb on + strand at 528.856 kb on + strand at 528.864 kb on + strand at 528.864 kb on - strand at 528.878 kb on - strand at 528.902 kb on + strand at 528.910 kb on - strand at 528.915 kb on - strand at 528.972 kb on + strand at 529.040 kb on - strand at 529.074 kb on - strand at 529.127 kb on + strand at 529.147 kb on + strand at 529.147 kb on + strand at 529.152 kb on + strand at 529.155 kb on - strand at 529.155 kb on - strand at 529.155 kb on - strand at 529.160 kb on - strand at 529.189 kb on - strand at 529.264 kb on + strand at 529.371 kb on - strand at 529.398 kb on + strand at 529.405 kb on + strand at 529.423 kb on + strand at 529.429 kb on - strand at 529.439 kb on - strand at 529.449 kb on - strand at 529.450 kb on - strand at 529.527 kb on - strand at 529.539 kb on - strand at 529.544 kb on - strand at 529.570 kb on + strand, within CCNA_00515 at 529.570 kb on + strand, within CCNA_00515 at 529.570 kb on + strand, within CCNA_00515 at 529.570 kb on + strand, within CCNA_00515 at 529.578 kb on - strand, within CCNA_00515 at 529.630 kb on + strand, within CCNA_00515 at 529.638 kb on - strand, within CCNA_00515 at 529.638 kb on - strand, within CCNA_00515 at 529.660 kb on + strand, within CCNA_00515 at 529.674 kb on - strand, within CCNA_00515 at 529.810 kb on + strand, within CCNA_00515 at 529.849 kb on - strand, within CCNA_00515 at 529.869 kb on + strand, within CCNA_00515 at 529.878 kb on + strand, within CCNA_00515 at 529.878 kb on + strand, within CCNA_00515 at 529.884 kb on + strand, within CCNA_00515 at 529.884 kb on + strand, within CCNA_00515 at 529.884 kb on + strand, within CCNA_00515 at 529.884 kb on + strand, within CCNA_00515 at 529.886 kb on - strand, within CCNA_00515 at 529.892 kb on - strand, within CCNA_00515 at 529.892 kb on - strand, within CCNA_00515 at 529.898 kb on - strand, within CCNA_00515 at 530.014 kb on + strand, within CCNA_00515 at 530.021 kb on - strand, within CCNA_00515 at 530.038 kb on + strand, within CCNA_00515 at 530.054 kb on + strand, within CCNA_00515 at 530.056 kb on - strand, within CCNA_00515 at 530.059 kb on + strand, within CCNA_00515 at 530.059 kb on + strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.147 kb on - strand, within CCNA_00515 at 530.159 kb on + strand, within CCNA_00515 at 530.165 kb on + strand, within CCNA_00515 at 530.165 kb on + strand, within CCNA_00515 at 530.165 kb on + strand, within CCNA_00515 at 530.167 kb on - strand, within CCNA_00515 at 530.170 kb on + strand, within CCNA_00515 at 530.173 kb on - strand, within CCNA_00515 at 530.173 kb on - strand, within CCNA_00515 at 530.175 kb on + strand, within CCNA_00515 at 530.241 kb on + strand, within CCNA_00515 at 530.296 kb on + strand, within CCNA_00515 at 530.298 kb on + strand, within CCNA_00515 at 530.298 kb on + strand, within CCNA_00515 at 530.377 kb on + strand, within CCNA_00515 at 530.378 kb on + strand, within CCNA_00515 at 530.434 kb on + strand, within CCNA_00515 at 530.442 kb on + strand, within CCNA_00515 at 530.442 kb on - strand, within CCNA_00515 at 530.480 kb on - strand, within CCNA_00515 at 530.588 kb on - strand, within CCNA_00515 at 530.629 kb on - strand, within CCNA_00515 at 530.652 kb on - strand, within CCNA_00515 at 530.661 kb on + strand, within CCNA_00515 at 530.681 kb on - strand, within CCNA_00515 at 530.742 kb on + strand, within CCNA_00515 at 530.743 kb on + strand, within CCNA_00515 at 530.748 kb on + strand, within CCNA_00515 at 530.748 kb on + strand, within CCNA_00515 at 530.751 kb on - strand, within CCNA_00515 at 530.808 kb on + strand, within CCNA_00515 at 530.810 kb on - strand, within CCNA_00515 at 530.822 kb on + strand, within CCNA_00515 at 530.822 kb on + strand, within CCNA_00515 at 530.866 kb on + strand, within CCNA_00515 at 530.866 kb on + strand, within CCNA_00515 at 530.874 kb on - strand, within CCNA_00515 at 530.874 kb on - strand, within CCNA_00515 at 530.874 kb on - strand, within CCNA_00515 at 530.874 kb on - strand, within CCNA_00515 at 530.879 kb on - strand, within CCNA_00515 at 530.900 kb on + strand, within CCNA_00515 at 530.915 kb on + strand, within CCNA_00515 at 530.923 kb on - strand, within CCNA_00515 at 530.925 kb on + strand, within CCNA_00515 at 530.933 kb on - strand, within CCNA_00515 at 530.933 kb on - strand, within CCNA_00515 at 530.933 kb on - strand, within CCNA_00515 at 530.940 kb on - strand, within CCNA_00515 at 530.969 kb on - strand, within CCNA_00515 at 531.011 kb on + strand, within CCNA_00515 at 531.018 kb on + strand, within CCNA_00515 at 531.019 kb on - strand, within CCNA_00515 at 531.027 kb on - strand, within CCNA_00515 at 531.036 kb on + strand, within CCNA_00515 at 531.067 kb on + strand, within CCNA_00515 at 531.067 kb on + strand, within CCNA_00515 at 531.067 kb on + strand, within CCNA_00515 at 531.067 kb on + strand, within CCNA_00515 at 531.127 kb on + strand, within CCNA_00515 at 531.135 kb on - strand, within CCNA_00515 at 531.234 kb on + strand, within CCNA_00515 at 531.244 kb on + strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.256 kb on + strand, within CCNA_00515 at 531.259 kb on + strand, within CCNA_00515 at 531.304 kb on - strand, within CCNA_00515 at 531.316 kb on + strand, within CCNA_00515 at 531.403 kb on + strand, within CCNA_00515 at 531.407 kb on - strand, within CCNA_00515 at 531.442 kb on - strand at 531.445 kb on + strand at 531.445 kb on + strand at 531.453 kb on - strand at 531.462 kb on - strand at 531.463 kb on - strand at 531.471 kb on + strand at 531.512 kb on - strand at 531.514 kb on - strand at 531.552 kb on + strand at 531.552 kb on + strand at 531.552 kb on + strand at 531.552 kb on + strand at 531.560 kb on - strand at 531.563 kb on + strand at 531.582 kb on - strand at 531.584 kb on + strand at 531.660 kb on - strand at 531.682 kb on + strand at 531.717 kb on + strand at 531.725 kb on - strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.747 kb on + strand at 531.755 kb on - strand at 531.755 kb on - strand at 531.755 kb on - strand at 531.825 kb on + strand, within CCNA_00516 at 531.825 kb on + strand, within CCNA_00516 at 531.833 kb on + strand, within CCNA_00516 at 531.850 kb on - strand, within CCNA_00516 at 531.870 kb on - strand, within CCNA_00516 at 531.872 kb on + strand, within CCNA_00516 at 531.872 kb on + strand, within CCNA_00516 at 531.872 kb on + strand, within CCNA_00516 at 531.880 kb on - strand, within CCNA_00516 at 531.880 kb on - strand, within CCNA_00516 at 531.880 kb on - strand, within CCNA_00516 at 531.880 kb on - strand, within CCNA_00516 at 531.882 kb on + strand, within CCNA_00516 at 531.887 kb on + strand, within CCNA_00516 at 531.890 kb on - strand, within CCNA_00516 at 531.890 kb on - strand, within CCNA_00516 at 531.895 kb on - strand, within CCNA_00516 at 531.907 kb on + strand, within CCNA_00516 at 531.915 kb on - strand, within CCNA_00516 at 531.915 kb on - strand, within CCNA_00516 at 531.915 kb on - strand, within CCNA_00516 at 531.939 kb on + strand, within CCNA_00516 at 531.939 kb on + strand, within CCNA_00516 at 531.947 kb on - strand, within CCNA_00516 at 531.947 kb on - strand, within CCNA_00516 at 531.960 kb on + strand, within CCNA_00516 at 531.967 kb on - strand, within CCNA_00516 at 532.014 kb on + strand, within CCNA_00516 at 532.019 kb on + strand, within CCNA_00516 at 532.024 kb on + strand, within CCNA_00516 at 532.037 kb on + strand, within CCNA_00516 at 532.070 kb on - strand, within CCNA_00516 at 532.070 kb on - strand, within CCNA_00516 at 532.087 kb on - strand, within CCNA_00516 at 532.109 kb on - strand, within CCNA_00516 at 532.113 kb on - strand, within CCNA_00516 at 532.177 kb on - strand, within CCNA_00516 at 532.249 kb on - strand, within CCNA_00516 at 532.266 kb on + strand, within CCNA_00516 at 532.272 kb on + strand, within CCNA_00516 at 532.275 kb on - strand, within CCNA_00516 at 532.284 kb on - strand, within CCNA_00516 at 532.298 kb on - strand, within CCNA_00516 at 532.334 kb on + strand, within CCNA_00516 at 532.337 kb on - strand, within CCNA_00516 at 532.355 kb on + strand, within CCNA_00516 at 532.355 kb on + strand, within CCNA_00516 at 532.355 kb on + strand, within CCNA_00516 at 532.363 kb on - strand at 532.383 kb on + strand at 532.403 kb on + strand at 532.461 kb on + strand at 532.461 kb on + strand at 532.461 kb on + strand at 532.474 kb on - strand at 532.501 kb on - strand, within CCNA_00517 at 532.501 kb on - strand, within CCNA_00517 at 532.501 kb on - strand, within CCNA_00517
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Benzethonium chloride 0.001 mM remove 528,330 + CCNA_00514 0.30 -1.0 528,337 - CCNA_00514 0.31 -0.5 528,338 - CCNA_00514 0.31 +0.6 528,369 + CCNA_00514 0.35 -1.1 528,405 + CCNA_00514 0.39 -0.8 528,405 + CCNA_00514 0.39 -0.4 528,413 - CCNA_00514 0.40 -0.6 528,424 + CCNA_00514 0.42 -0.5 528,424 + CCNA_00514 0.42 -0.8 528,432 - CCNA_00514 0.43 -0.6 528,432 - CCNA_00514 0.43 -0.5 528,436 + CCNA_00514 0.43 -0.6 528,436 + CCNA_00514 0.43 -0.8 528,621 + CCNA_00514 0.65 -0.2 528,645 - CCNA_00514 0.68 -0.1 528,645 - CCNA_00514 0.68 -2.2 528,662 + CCNA_00514 0.70 -1.0 528,690 + CCNA_00514 0.74 +1.2 528,690 + CCNA_00514 0.74 -0.2 528,698 - CCNA_00514 0.75 -0.1 528,713 - CCNA_00514 0.76 +0.5 528,757 - CCNA_00514 0.82 +0.0 528,777 + CCNA_00514 0.84 +1.2 528,802 - CCNA_00514 0.87 -1.1 528,832 + +0.8 528,856 + +2.5 528,864 + -1.0 528,864 - +0.6 528,878 - +1.2 528,902 + -1.0 528,910 - +0.5 528,915 - -0.8 528,972 + +0.4 529,040 - -0.8 529,074 - +0.6 529,127 + -0.8 529,147 + +0.1 529,147 + +0.0 529,152 + +1.5 529,155 - -1.4 529,155 - +2.2 529,155 - -1.4 529,160 - -0.4 529,189 - -0.3 529,264 + +0.6 529,371 - -0.3 529,398 + +1.3 529,405 + +1.0 529,423 + -0.4 529,429 - -0.9 529,439 - +1.0 529,449 - -2.2 529,450 - -0.6 529,527 - -0.4 529,539 - -1.8 529,544 - -0.1 529,570 + CCNA_00515 0.11 +1.1 529,570 + CCNA_00515 0.11 +1.6 529,570 + CCNA_00515 0.11 +1.7 529,570 + CCNA_00515 0.11 +1.4 529,578 - CCNA_00515 0.11 -0.7 529,630 + CCNA_00515 0.13 +0.4 529,638 - CCNA_00515 0.13 +1.1 529,638 - CCNA_00515 0.13 +2.2 529,660 + CCNA_00515 0.14 -0.6 529,674 - CCNA_00515 0.15 +0.2 529,810 + CCNA_00515 0.21 +1.2 529,849 - CCNA_00515 0.22 -0.8 529,869 + CCNA_00515 0.23 +0.3 529,878 + CCNA_00515 0.24 +0.1 529,878 + CCNA_00515 0.24 +0.3 529,884 + CCNA_00515 0.24 +0.5 529,884 + CCNA_00515 0.24 +1.9 529,884 + CCNA_00515 0.24 +0.1 529,884 + CCNA_00515 0.24 +0.6 529,886 - CCNA_00515 0.24 +0.5 529,892 - CCNA_00515 0.24 +0.9 529,892 - CCNA_00515 0.24 +0.3 529,898 - CCNA_00515 0.25 -2.5 530,014 + CCNA_00515 0.30 +1.3 530,021 - CCNA_00515 0.30 -2.2 530,038 + CCNA_00515 0.31 +1.6 530,054 + CCNA_00515 0.31 +0.6 530,056 - CCNA_00515 0.31 -1.1 530,059 + CCNA_00515 0.31 +0.8 530,059 + CCNA_00515 0.31 +1.1 530,067 - CCNA_00515 0.32 -2.3 530,067 - CCNA_00515 0.32 -1.8 530,067 - CCNA_00515 0.32 +1.1 530,067 - CCNA_00515 0.32 -1.9 530,067 - CCNA_00515 0.32 -0.1 530,067 - CCNA_00515 0.32 -0.6 530,067 - CCNA_00515 0.32 -1.4 530,147 - CCNA_00515 0.35 -0.1 530,159 + CCNA_00515 0.36 +0.4 530,165 + CCNA_00515 0.36 +1.1 530,165 + CCNA_00515 0.36 +0.8 530,165 + CCNA_00515 0.36 -0.0 530,167 - CCNA_00515 0.36 -0.2 530,170 + CCNA_00515 0.36 +1.2 530,173 - CCNA_00515 0.36 +0.3 530,173 - CCNA_00515 0.36 +1.4 530,175 + CCNA_00515 0.36 +1.1 530,241 + CCNA_00515 0.39 +2.5 530,296 + CCNA_00515 0.42 +1.5 530,298 + CCNA_00515 0.42 +1.5 530,298 + CCNA_00515 0.42 +1.6 530,377 + CCNA_00515 0.45 +0.9 530,378 + CCNA_00515 0.45 +0.4 530,434 + CCNA_00515 0.48 +1.5 530,442 + CCNA_00515 0.48 +1.7 530,442 - CCNA_00515 0.48 -1.1 530,480 - CCNA_00515 0.50 -1.0 530,588 - CCNA_00515 0.54 +0.3 530,629 - CCNA_00515 0.56 +0.6 530,652 - CCNA_00515 0.57 -0.1 530,661 + CCNA_00515 0.57 +0.3 530,681 - CCNA_00515 0.58 -0.7 530,742 + CCNA_00515 0.61 +1.7 530,743 + CCNA_00515 0.61 +0.3 530,748 + CCNA_00515 0.61 +1.4 530,748 + CCNA_00515 0.61 +1.5 530,751 - CCNA_00515 0.61 -1.5 530,808 + CCNA_00515 0.64 +0.2 530,810 - CCNA_00515 0.64 -1.2 530,822 + CCNA_00515 0.64 +1.9 530,822 + CCNA_00515 0.64 +0.2 530,866 + CCNA_00515 0.66 +0.7 530,866 + CCNA_00515 0.66 +1.8 530,874 - CCNA_00515 0.66 -0.9 530,874 - CCNA_00515 0.66 +0.4 530,874 - CCNA_00515 0.66 +1.1 530,874 - CCNA_00515 0.66 -1.8 530,879 - CCNA_00515 0.67 +1.6 530,900 + CCNA_00515 0.68 +0.7 530,915 + CCNA_00515 0.68 +0.6 530,923 - CCNA_00515 0.69 +0.8 530,925 + CCNA_00515 0.69 +1.6 530,933 - CCNA_00515 0.69 -0.5 530,933 - CCNA_00515 0.69 +1.2 530,933 - CCNA_00515 0.69 -0.1 530,940 - CCNA_00515 0.69 +1.1 530,969 - CCNA_00515 0.70 +0.2 531,011 + CCNA_00515 0.72 +2.2 531,018 + CCNA_00515 0.73 +0.8 531,019 - CCNA_00515 0.73 -0.4 531,027 - CCNA_00515 0.73 +0.9 531,036 + CCNA_00515 0.73 +0.7 531,067 + CCNA_00515 0.75 +2.3 531,067 + CCNA_00515 0.75 +1.7 531,067 + CCNA_00515 0.75 +2.0 531,067 + CCNA_00515 0.75 +2.4 531,127 + CCNA_00515 0.77 +2.5 531,135 - CCNA_00515 0.78 +1.0 531,234 + CCNA_00515 0.82 +0.8 531,244 + CCNA_00515 0.82 -0.4 531,252 - CCNA_00515 0.83 +3.8 531,252 - CCNA_00515 0.83 +1.2 531,252 - CCNA_00515 0.83 +0.6 531,252 - CCNA_00515 0.83 +1.2 531,252 - CCNA_00515 0.83 -0.9 531,252 - CCNA_00515 0.83 +1.8 531,256 + CCNA_00515 0.83 +1.1 531,259 + CCNA_00515 0.83 +1.2 531,304 - CCNA_00515 0.85 -0.2 531,316 + CCNA_00515 0.85 +1.9 531,403 + CCNA_00515 0.89 +1.5 531,407 - CCNA_00515 0.89 +0.1 531,442 - -1.2 531,445 + +2.0 531,445 + +1.2 531,453 - -0.6 531,462 - -0.2 531,463 - +0.3 531,471 + +0.7 531,512 - +0.2 531,514 - +0.2 531,552 + +0.2 531,552 + +1.2 531,552 + +2.2 531,552 + +2.0 531,560 - +1.7 531,563 + +1.9 531,582 - +0.0 531,584 + +2.7 531,660 - +2.2 531,682 + -0.3 531,717 + +3.8 531,725 - -0.4 531,746 + -1.9 531,746 + -0.9 531,747 + +1.1 531,755 - +0.9 531,755 - +0.4 531,755 - +0.2 531,825 + CCNA_00516 0.19 +1.0 531,825 + CCNA_00516 0.19 +0.8 531,833 + CCNA_00516 0.20 -1.5 531,850 - CCNA_00516 0.22 +0.9 531,870 - CCNA_00516 0.25 -0.4 531,872 + CCNA_00516 0.25 -1.9 531,872 + CCNA_00516 0.25 -2.2 531,872 + CCNA_00516 0.25 +0.2 531,880 - CCNA_00516 0.26 +1.4 531,880 - CCNA_00516 0.26 +1.0 531,880 - CCNA_00516 0.26 +0.6 531,880 - CCNA_00516 0.26 +0.5 531,882 + CCNA_00516 0.27 +1.8 531,887 + CCNA_00516 0.27 +0.2 531,890 - CCNA_00516 0.28 +0.6 531,890 - CCNA_00516 0.28 -0.4 531,895 - CCNA_00516 0.28 +1.8 531,907 + CCNA_00516 0.30 -0.6 531,915 - CCNA_00516 0.31 +0.4 531,915 - CCNA_00516 0.31 +0.3 531,915 - CCNA_00516 0.31 -0.1 531,939 + CCNA_00516 0.34 -0.9 531,939 + CCNA_00516 0.34 +0.1 531,947 - CCNA_00516 0.35 +0.6 531,947 - CCNA_00516 0.35 +1.0 531,960 + CCNA_00516 0.37 -0.4 531,967 - CCNA_00516 0.38 +0.0 532,014 + CCNA_00516 0.44 -0.4 532,019 + CCNA_00516 0.45 -0.9 532,024 + CCNA_00516 0.45 -0.3 532,037 + CCNA_00516 0.47 -1.6 532,070 - CCNA_00516 0.52 -0.9 532,070 - CCNA_00516 0.52 -0.5 532,087 - CCNA_00516 0.54 -0.2 532,109 - CCNA_00516 0.57 +0.0 532,113 - CCNA_00516 0.57 +0.9 532,177 - CCNA_00516 0.66 -1.1 532,249 - CCNA_00516 0.75 -0.4 532,266 + CCNA_00516 0.78 +0.8 532,272 + CCNA_00516 0.79 -1.9 532,275 - CCNA_00516 0.79 +2.2 532,284 - CCNA_00516 0.80 +0.9 532,298 - CCNA_00516 0.82 +1.8 532,334 + CCNA_00516 0.87 +1.2 532,337 - CCNA_00516 0.87 +0.9 532,355 + CCNA_00516 0.90 -1.9 532,355 + CCNA_00516 0.90 +1.6 532,355 + CCNA_00516 0.90 +1.2 532,363 - +0.4 532,383 + -0.6 532,403 + +1.2 532,461 + +2.3 532,461 + +2.1 532,461 + +2.8 532,474 - +0.3 532,501 - CCNA_00517 0.11 +2.8 532,501 - CCNA_00517 0.11 -0.4 532,501 - CCNA_00517 0.11 +1.2
Or see this region's nucleotide sequence