Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS33305 and AZOBR_RS33310 are separated by 7 nucleotides AZOBR_RS33310 and AZOBR_RS33315 are separated by 83 nucleotides AZOBR_RS33315 and AZOBR_RS33320 are separated by 151 nucleotides AZOBR_RS33320 and AZOBR_RS33325 are separated by 3 nucleotides
AZOBR_RS33305: AZOBR_RS33305 - hypothetical protein, at 164,792 to 165,361
_RS33305
AZOBR_RS33310: AZOBR_RS33310 - hypothetical protein, at 165,369 to 165,809
_RS33310
AZOBR_RS33315: AZOBR_RS33315 - hypothetical protein, at 165,893 to 166,873
_RS33315
AZOBR_RS33320: AZOBR_RS33320 - hypothetical protein, at 167,025 to 167,465
_RS33320
AZOBR_RS33325: AZOBR_RS33325 - hypothetical protein, at 167,469 to 167,954
_RS33325
Position (kb) 165
166
167 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 165.120 kb on - strand, within AZOBR_RS33305 at 165.120 kb on - strand, within AZOBR_RS33305 at 165.275 kb on + strand, within AZOBR_RS33305 at 165.275 kb on + strand, within AZOBR_RS33305 at 165.276 kb on - strand, within AZOBR_RS33305 at 165.507 kb on + strand, within AZOBR_RS33310 at 165.624 kb on + strand, within AZOBR_RS33310 at 166.043 kb on + strand, within AZOBR_RS33315 at 166.358 kb on + strand at 166.358 kb on + strand, within AZOBR_RS33315 at 166.358 kb on + strand, within AZOBR_RS33315 at 166.359 kb on - strand, within AZOBR_RS33315 at 166.359 kb on - strand, within AZOBR_RS33315 at 166.454 kb on + strand, within AZOBR_RS33315 at 166.454 kb on + strand, within AZOBR_RS33315 at 166.454 kb on + strand, within AZOBR_RS33315 at 166.454 kb on + strand, within AZOBR_RS33315 at 166.454 kb on + strand, within AZOBR_RS33315 at 166.455 kb on - strand, within AZOBR_RS33315 at 166.455 kb on - strand, within AZOBR_RS33315 at 166.455 kb on - strand, within AZOBR_RS33315 at 166.455 kb on - strand, within AZOBR_RS33315 at 166.475 kb on - strand, within AZOBR_RS33315 at 166.542 kb on - strand, within AZOBR_RS33315 at 166.542 kb on - strand, within AZOBR_RS33315 at 166.568 kb on + strand, within AZOBR_RS33315 at 166.572 kb on - strand, within AZOBR_RS33315 at 166.572 kb on - strand, within AZOBR_RS33315 at 166.909 kb on + strand at 166.914 kb on - strand at 166.947 kb on + strand at 166.947 kb on + strand at 166.948 kb on - strand at 166.997 kb on + strand at 167.166 kb on + strand, within AZOBR_RS33320 at 167.311 kb on - strand, within AZOBR_RS33320 at 167.463 kb on + strand at 167.464 kb on - strand at 167.586 kb on + strand, within AZOBR_RS33325 at 167.586 kb on + strand, within AZOBR_RS33325 at 167.775 kb on + strand, within AZOBR_RS33325
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 165,120 - AZOBR_RS33305 0.58 +0.5 165,120 - AZOBR_RS33305 0.58 +1.9 165,275 + AZOBR_RS33305 0.85 +0.4 165,275 + AZOBR_RS33305 0.85 -0.4 165,276 - AZOBR_RS33305 0.85 +0.1 165,507 + AZOBR_RS33310 0.31 -0.1 165,624 + AZOBR_RS33310 0.58 -1.8 166,043 + AZOBR_RS33315 0.15 -3.0 166,358 + -0.9 166,358 + AZOBR_RS33315 0.47 -0.9 166,358 + AZOBR_RS33315 0.47 -3.5 166,359 - AZOBR_RS33315 0.48 -5.7 166,359 - AZOBR_RS33315 0.48 -2.2 166,454 + AZOBR_RS33315 0.57 -3.5 166,454 + AZOBR_RS33315 0.57 -3.8 166,454 + AZOBR_RS33315 0.57 -3.6 166,454 + AZOBR_RS33315 0.57 -5.5 166,454 + AZOBR_RS33315 0.57 -5.8 166,455 - AZOBR_RS33315 0.57 -1.5 166,455 - AZOBR_RS33315 0.57 -4.4 166,455 - AZOBR_RS33315 0.57 -4.2 166,455 - AZOBR_RS33315 0.57 -0.8 166,475 - AZOBR_RS33315 0.59 -5.5 166,542 - AZOBR_RS33315 0.66 -6.0 166,542 - AZOBR_RS33315 0.66 -7.3 166,568 + AZOBR_RS33315 0.69 -1.9 166,572 - AZOBR_RS33315 0.69 -4.9 166,572 - AZOBR_RS33315 0.69 -4.0 166,909 + +0.9 166,914 - -0.7 166,947 + +1.8 166,947 + -0.9 166,948 - -3.7 166,997 + -0.7 167,166 + AZOBR_RS33320 0.32 -1.0 167,311 - AZOBR_RS33320 0.65 -1.9 167,463 + -0.3 167,464 - +1.1 167,586 + AZOBR_RS33325 0.24 -0.6 167,586 + AZOBR_RS33325 0.24 +1.1 167,775 + AZOBR_RS33325 0.63 -0.7
Or see this region's nucleotide sequence