Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS23280 and AZOBR_RS23285 are separated by 302 nucleotides AZOBR_RS23285 and AZOBR_RS23290 are separated by 2 nucleotides
AZOBR_RS23280: AZOBR_RS23280 - carboxymuconolactone decarboxylase, at 249,848 to 250,168
_RS23280
AZOBR_RS23285: AZOBR_RS23285 - Dak phosphatase, at 250,471 to 251,085
_RS23285
AZOBR_RS23290: AZOBR_RS23290 - dihydroxyacetone kinase subunit K, at 251,088 to 252,086
_RS23290
Position (kb) 250
251
252 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 249.548 kb on + strand at 249.549 kb on - strand at 249.549 kb on - strand at 249.549 kb on - strand at 249.549 kb on - strand at 249.665 kb on - strand at 249.672 kb on + strand at 249.674 kb on + strand at 249.674 kb on + strand at 249.675 kb on - strand at 249.675 kb on - strand at 249.867 kb on + strand at 249.867 kb on + strand at 249.867 kb on + strand at 249.868 kb on - strand at 249.868 kb on - strand at 249.868 kb on - strand at 249.868 kb on - strand at 249.868 kb on - strand at 249.986 kb on + strand, within AZOBR_RS23280 at 250.154 kb on + strand at 250.166 kb on + strand at 250.166 kb on + strand at 250.166 kb on + strand at 250.167 kb on - strand at 250.191 kb on + strand at 250.191 kb on + strand at 250.192 kb on - strand at 250.192 kb on - strand at 250.192 kb on - strand at 250.192 kb on - strand at 250.556 kb on + strand, within AZOBR_RS23285 at 250.556 kb on + strand, within AZOBR_RS23285 at 250.556 kb on + strand, within AZOBR_RS23285 at 250.557 kb on - strand, within AZOBR_RS23285 at 250.557 kb on - strand, within AZOBR_RS23285 at 250.557 kb on - strand, within AZOBR_RS23285 at 250.557 kb on - strand, within AZOBR_RS23285 at 250.557 kb on - strand, within AZOBR_RS23285 at 250.557 kb on - strand, within AZOBR_RS23285 at 250.557 kb on - strand, within AZOBR_RS23285 at 250.557 kb on - strand, within AZOBR_RS23285 at 250.708 kb on - strand, within AZOBR_RS23285 at 250.845 kb on + strand, within AZOBR_RS23285 at 251.200 kb on + strand, within AZOBR_RS23290 at 251.200 kb on + strand, within AZOBR_RS23290 at 251.200 kb on + strand, within AZOBR_RS23290 at 251.200 kb on + strand, within AZOBR_RS23290 at 251.614 kb on + strand, within AZOBR_RS23290 at 251.614 kb on + strand, within AZOBR_RS23290 at 251.615 kb on - strand, within AZOBR_RS23290 at 251.620 kb on + strand, within AZOBR_RS23290 at 251.620 kb on + strand, within AZOBR_RS23290 at 251.620 kb on + strand, within AZOBR_RS23290 at 251.767 kb on + strand, within AZOBR_RS23290 at 251.767 kb on + strand, within AZOBR_RS23290 at 251.768 kb on - strand, within AZOBR_RS23290 at 251.776 kb on + strand, within AZOBR_RS23290 at 252.056 kb on - strand at 252.056 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 249,548 + -2.0 249,549 - -1.4 249,549 - +0.8 249,549 - +2.0 249,549 - -0.1 249,665 - -1.4 249,672 + +3.1 249,674 + -0.1 249,674 + +0.5 249,675 - -1.8 249,675 - +1.3 249,867 + -1.1 249,867 + +0.9 249,867 + -2.4 249,868 - -0.4 249,868 - +0.6 249,868 - -3.9 249,868 - +1.0 249,868 - -0.9 249,986 + AZOBR_RS23280 0.43 -1.7 250,154 + -1.5 250,166 + -0.8 250,166 + +0.9 250,166 + -2.5 250,167 - +0.1 250,191 + -2.9 250,191 + +0.7 250,192 - -0.8 250,192 - -0.4 250,192 - +0.8 250,192 - -0.3 250,556 + AZOBR_RS23285 0.14 -2.4 250,556 + AZOBR_RS23285 0.14 -1.4 250,556 + AZOBR_RS23285 0.14 -0.0 250,557 - AZOBR_RS23285 0.14 -1.1 250,557 - AZOBR_RS23285 0.14 +0.8 250,557 - AZOBR_RS23285 0.14 +0.1 250,557 - AZOBR_RS23285 0.14 +1.2 250,557 - AZOBR_RS23285 0.14 +0.4 250,557 - AZOBR_RS23285 0.14 -0.8 250,557 - AZOBR_RS23285 0.14 -1.5 250,557 - AZOBR_RS23285 0.14 +1.9 250,708 - AZOBR_RS23285 0.39 -4.8 250,845 + AZOBR_RS23285 0.61 -1.3 251,200 + AZOBR_RS23290 0.11 -0.7 251,200 + AZOBR_RS23290 0.11 -0.6 251,200 + AZOBR_RS23290 0.11 -3.6 251,200 + AZOBR_RS23290 0.11 -0.4 251,614 + AZOBR_RS23290 0.53 +1.9 251,614 + AZOBR_RS23290 0.53 -1.3 251,615 - AZOBR_RS23290 0.53 -1.9 251,620 + AZOBR_RS23290 0.53 +1.6 251,620 + AZOBR_RS23290 0.53 +1.6 251,620 + AZOBR_RS23290 0.53 -2.3 251,767 + AZOBR_RS23290 0.68 +1.7 251,767 + AZOBR_RS23290 0.68 +2.4 251,768 - AZOBR_RS23290 0.68 -0.5 251,776 + AZOBR_RS23290 0.69 -0.3 252,056 - -2.3 252,056 - -3.8
Or see this region's nucleotide sequence