Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS23245 and AZOBR_RS23250 are separated by 84 nucleotides AZOBR_RS23250 and AZOBR_RS23255 are separated by 45 nucleotides AZOBR_RS23255 and AZOBR_RS23260 overlap by 64 nucleotides
AZOBR_RS23245: AZOBR_RS23245 - hypothetical protein, at 227,935 to 228,477
_RS23245
AZOBR_RS23250: AZOBR_RS23250 - SapC family protein, at 228,562 to 229,287
_RS23250
AZOBR_RS23255: AZOBR_RS23255 - hypothetical protein, at 229,333 to 229,632
_RS23255
AZOBR_RS23260: AZOBR_RS23260 - hypothetical protein, at 229,569 to 236,186
_RS23260
Position (kb) 229
230 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 228.425 kb on - strand at 228.425 kb on - strand at 228.626 kb on + strand at 228.627 kb on - strand at 228.627 kb on - strand at 228.627 kb on - strand at 228.813 kb on + strand, within AZOBR_RS23250 at 228.813 kb on + strand, within AZOBR_RS23250 at 228.813 kb on + strand, within AZOBR_RS23250 at 228.813 kb on + strand, within AZOBR_RS23250 at 228.814 kb on - strand, within AZOBR_RS23250 at 228.814 kb on - strand, within AZOBR_RS23250 at 228.814 kb on - strand, within AZOBR_RS23250 at 228.815 kb on + strand, within AZOBR_RS23250 at 228.815 kb on + strand, within AZOBR_RS23250 at 228.816 kb on - strand, within AZOBR_RS23250 at 228.816 kb on - strand, within AZOBR_RS23250 at 229.001 kb on + strand, within AZOBR_RS23250 at 229.013 kb on + strand, within AZOBR_RS23250 at 229.014 kb on - strand, within AZOBR_RS23250 at 229.014 kb on - strand, within AZOBR_RS23250 at 229.014 kb on - strand, within AZOBR_RS23250 at 229.014 kb on - strand, within AZOBR_RS23250 at 229.259 kb on + strand at 229.259 kb on + strand at 229.260 kb on - strand at 229.260 kb on - strand at 229.260 kb on - strand at 229.295 kb on + strand at 229.295 kb on + strand at 229.307 kb on - strand at 229.692 kb on + strand at 229.693 kb on - strand at 229.693 kb on - strand at 229.693 kb on - strand at 229.693 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 228,425 - +0.3 228,425 - -0.1 228,626 + -2.2 228,627 - -2.1 228,627 - +1.0 228,627 - +1.0 228,813 + AZOBR_RS23250 0.35 -1.4 228,813 + AZOBR_RS23250 0.35 -1.8 228,813 + AZOBR_RS23250 0.35 -2.6 228,813 + AZOBR_RS23250 0.35 +0.8 228,814 - AZOBR_RS23250 0.35 +0.2 228,814 - AZOBR_RS23250 0.35 -2.9 228,814 - AZOBR_RS23250 0.35 -0.8 228,815 + AZOBR_RS23250 0.35 +0.2 228,815 + AZOBR_RS23250 0.35 -3.8 228,816 - AZOBR_RS23250 0.35 +1.2 228,816 - AZOBR_RS23250 0.35 -4.5 229,001 + AZOBR_RS23250 0.60 +0.3 229,013 + AZOBR_RS23250 0.62 +0.3 229,014 - AZOBR_RS23250 0.62 -0.4 229,014 - AZOBR_RS23250 0.62 -2.7 229,014 - AZOBR_RS23250 0.62 -3.6 229,014 - AZOBR_RS23250 0.62 +0.0 229,259 + +0.9 229,259 + -0.6 229,260 - +0.3 229,260 - -3.0 229,260 - -1.1 229,295 + -2.1 229,295 + -0.1 229,307 - -0.2 229,692 + +0.8 229,693 - -2.5 229,693 - -2.5 229,693 - -1.4 229,693 - -0.1
Or see this region's nucleotide sequence