Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS13535 and AZOBR_RS13545 are separated by 504 nucleotides AZOBR_RS13545 and AZOBR_RS13550 overlap by 1 nucleotides
AZOBR_RS13535: AZOBR_RS13535 - hydrolase, at 2,872,229 to 2,872,630
_RS13535
AZOBR_RS13545: AZOBR_RS13545 - hypothetical protein, at 2,873,135 to 2,874,676
_RS13545
AZOBR_RS13550: AZOBR_RS13550 - hypothetical protein, at 2,874,676 to 2,876,706
_RS13550
Position (kb)
2873
2874
2875 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2872.245 kb on + strand at 2872.245 kb on + strand at 2872.246 kb on - strand at 2872.742 kb on + strand at 2872.847 kb on + strand at 2872.847 kb on + strand at 2872.847 kb on + strand at 2872.847 kb on + strand at 2872.848 kb on - strand at 2872.848 kb on - strand at 2872.871 kb on + strand at 2872.871 kb on + strand at 2872.872 kb on - strand at 2872.872 kb on - strand at 2873.280 kb on + strand at 2873.607 kb on + strand, within AZOBR_RS13545 at 2873.607 kb on + strand, within AZOBR_RS13545 at 2873.608 kb on - strand, within AZOBR_RS13545 at 2873.625 kb on + strand, within AZOBR_RS13545 at 2873.625 kb on + strand, within AZOBR_RS13545 at 2873.625 kb on + strand, within AZOBR_RS13545 at 2874.058 kb on - strand, within AZOBR_RS13545 at 2874.066 kb on + strand, within AZOBR_RS13545 at 2874.066 kb on + strand, within AZOBR_RS13545 at 2874.066 kb on + strand, within AZOBR_RS13545 at 2874.067 kb on - strand, within AZOBR_RS13545 at 2874.067 kb on - strand, within AZOBR_RS13545 at 2874.069 kb on + strand, within AZOBR_RS13545 at 2874.069 kb on + strand, within AZOBR_RS13545 at 2874.070 kb on - strand, within AZOBR_RS13545 at 2874.070 kb on - strand, within AZOBR_RS13545 at 2874.070 kb on - strand, within AZOBR_RS13545 at 2874.070 kb on - strand, within AZOBR_RS13545 at 2874.090 kb on + strand, within AZOBR_RS13545 at 2874.090 kb on + strand, within AZOBR_RS13545 at 2874.091 kb on - strand, within AZOBR_RS13545 at 2874.091 kb on - strand, within AZOBR_RS13545 at 2874.091 kb on - strand, within AZOBR_RS13545 at 2874.091 kb on - strand, within AZOBR_RS13545 at 2874.120 kb on + strand, within AZOBR_RS13545 at 2874.120 kb on + strand, within AZOBR_RS13545 at 2874.121 kb on - strand, within AZOBR_RS13545 at 2874.121 kb on - strand, within AZOBR_RS13545 at 2874.121 kb on - strand, within AZOBR_RS13545 at 2874.121 kb on - strand, within AZOBR_RS13545 at 2874.454 kb on - strand, within AZOBR_RS13545 at 2874.915 kb on - strand, within AZOBR_RS13550 at 2875.041 kb on - strand, within AZOBR_RS13550 at 2875.196 kb on + strand, within AZOBR_RS13550 at 2875.211 kb on + strand, within AZOBR_RS13550 at 2875.211 kb on + strand, within AZOBR_RS13550 at 2875.212 kb on - strand, within AZOBR_RS13550 at 2875.212 kb on - strand, within AZOBR_RS13550 at 2875.212 kb on - strand, within AZOBR_RS13550 at 2875.265 kb on + strand, within AZOBR_RS13550 at 2875.266 kb on - strand, within AZOBR_RS13550 at 2875.266 kb on - strand, within AZOBR_RS13550 at 2875.266 kb on - strand, within AZOBR_RS13550 at 2875.277 kb on + strand, within AZOBR_RS13550 at 2875.362 kb on - strand, within AZOBR_RS13550 at 2875.382 kb on + strand, within AZOBR_RS13550 at 2875.382 kb on + strand, within AZOBR_RS13550 at 2875.382 kb on + strand, within AZOBR_RS13550 at 2875.383 kb on - strand, within AZOBR_RS13550 at 2875.383 kb on - strand, within AZOBR_RS13550 at 2875.383 kb on - strand, within AZOBR_RS13550 at 2875.383 kb on - strand, within AZOBR_RS13550 at 2875.485 kb on - strand, within AZOBR_RS13550 at 2875.586 kb on + strand, within AZOBR_RS13550 at 2875.586 kb on + strand, within AZOBR_RS13550 at 2875.586 kb on + strand, within AZOBR_RS13550 at 2875.586 kb on + strand, within AZOBR_RS13550 at 2875.586 kb on + strand, within AZOBR_RS13550 at 2875.586 kb on + strand, within AZOBR_RS13550 at 2875.587 kb on - strand, within AZOBR_RS13550 at 2875.587 kb on - strand, within AZOBR_RS13550 at 2875.587 kb on - strand, within AZOBR_RS13550
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,872,245 + -0.7 2,872,245 + -0.1 2,872,246 - -0.3 2,872,742 + -0.4 2,872,847 + -4.3 2,872,847 + -0.9 2,872,847 + +0.1 2,872,847 + +3.8 2,872,848 - -1.4 2,872,848 - -1.8 2,872,871 + +1.2 2,872,871 + +0.3 2,872,872 - -2.4 2,872,872 - -0.8 2,873,280 + +0.9 2,873,607 + AZOBR_RS13545 0.31 +0.0 2,873,607 + AZOBR_RS13545 0.31 +0.7 2,873,608 - AZOBR_RS13545 0.31 -0.8 2,873,625 + AZOBR_RS13545 0.32 -0.6 2,873,625 + AZOBR_RS13545 0.32 -0.2 2,873,625 + AZOBR_RS13545 0.32 +1.2 2,874,058 - AZOBR_RS13545 0.60 -0.4 2,874,066 + AZOBR_RS13545 0.60 -0.6 2,874,066 + AZOBR_RS13545 0.60 -2.6 2,874,066 + AZOBR_RS13545 0.60 -2.6 2,874,067 - AZOBR_RS13545 0.60 +1.1 2,874,067 - AZOBR_RS13545 0.60 -0.6 2,874,069 + AZOBR_RS13545 0.61 -2.1 2,874,069 + AZOBR_RS13545 0.61 +0.2 2,874,070 - AZOBR_RS13545 0.61 -0.3 2,874,070 - AZOBR_RS13545 0.61 -1.6 2,874,070 - AZOBR_RS13545 0.61 -0.0 2,874,070 - AZOBR_RS13545 0.61 -0.1 2,874,090 + AZOBR_RS13545 0.62 -2.3 2,874,090 + AZOBR_RS13545 0.62 -1.4 2,874,091 - AZOBR_RS13545 0.62 -0.2 2,874,091 - AZOBR_RS13545 0.62 +0.0 2,874,091 - AZOBR_RS13545 0.62 +1.4 2,874,091 - AZOBR_RS13545 0.62 -0.5 2,874,120 + AZOBR_RS13545 0.64 -1.5 2,874,120 + AZOBR_RS13545 0.64 -2.5 2,874,121 - AZOBR_RS13545 0.64 -0.0 2,874,121 - AZOBR_RS13545 0.64 -2.1 2,874,121 - AZOBR_RS13545 0.64 +0.0 2,874,121 - AZOBR_RS13545 0.64 -0.7 2,874,454 - AZOBR_RS13545 0.86 +0.3 2,874,915 - AZOBR_RS13550 0.12 -2.3 2,875,041 - AZOBR_RS13550 0.18 -0.5 2,875,196 + AZOBR_RS13550 0.26 -1.6 2,875,211 + AZOBR_RS13550 0.26 +0.4 2,875,211 + AZOBR_RS13550 0.26 -2.4 2,875,212 - AZOBR_RS13550 0.26 +1.7 2,875,212 - AZOBR_RS13550 0.26 -1.8 2,875,212 - AZOBR_RS13550 0.26 +0.8 2,875,265 + AZOBR_RS13550 0.29 +0.2 2,875,266 - AZOBR_RS13550 0.29 +0.4 2,875,266 - AZOBR_RS13550 0.29 -3.2 2,875,266 - AZOBR_RS13550 0.29 +0.2 2,875,277 + AZOBR_RS13550 0.30 -1.5 2,875,362 - AZOBR_RS13550 0.34 -1.9 2,875,382 + AZOBR_RS13550 0.35 -2.0 2,875,382 + AZOBR_RS13550 0.35 -0.1 2,875,382 + AZOBR_RS13550 0.35 -1.5 2,875,383 - AZOBR_RS13550 0.35 -0.8 2,875,383 - AZOBR_RS13550 0.35 -0.9 2,875,383 - AZOBR_RS13550 0.35 +0.1 2,875,383 - AZOBR_RS13550 0.35 +1.7 2,875,485 - AZOBR_RS13550 0.40 +0.1 2,875,586 + AZOBR_RS13550 0.45 +0.0 2,875,586 + AZOBR_RS13550 0.45 +0.7 2,875,586 + AZOBR_RS13550 0.45 -2.6 2,875,586 + AZOBR_RS13550 0.45 -3.8 2,875,586 + AZOBR_RS13550 0.45 -2.4 2,875,586 + AZOBR_RS13550 0.45 +0.1 2,875,587 - AZOBR_RS13550 0.45 +0.2 2,875,587 - AZOBR_RS13550 0.45 +1.4 2,875,587 - AZOBR_RS13550 0.45 -3.6
Or see this region's nucleotide sequence