Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS04255 and AZOBR_RS04260 are separated by 131 nucleotides AZOBR_RS04260 and AZOBR_RS04265 are separated by 346 nucleotides
AZOBR_RS04255: AZOBR_RS04255 - hypothetical protein, at 897,750 to 898,715
_RS04255
AZOBR_RS04260: AZOBR_RS04260 - hypothetical protein, at 898,847 to 899,227
_RS04260
AZOBR_RS04265: AZOBR_RS04265 - 2-isopropylmalate synthase, at 899,574 to 901,178
_RS04265
Position (kb) 898
899
900 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 897.917 kb on - strand, within AZOBR_RS04255 at 897.917 kb on - strand, within AZOBR_RS04255 at 897.917 kb on - strand, within AZOBR_RS04255 at 898.075 kb on + strand, within AZOBR_RS04255 at 898.075 kb on + strand, within AZOBR_RS04255 at 898.076 kb on - strand, within AZOBR_RS04255 at 898.150 kb on + strand, within AZOBR_RS04255 at 898.150 kb on + strand, within AZOBR_RS04255 at 898.150 kb on + strand, within AZOBR_RS04255 at 898.150 kb on + strand, within AZOBR_RS04255 at 898.151 kb on - strand, within AZOBR_RS04255 at 898.151 kb on - strand, within AZOBR_RS04255 at 898.151 kb on - strand, within AZOBR_RS04255 at 898.151 kb on - strand, within AZOBR_RS04255 at 898.151 kb on - strand, within AZOBR_RS04255 at 898.151 kb on - strand, within AZOBR_RS04255 at 898.151 kb on - strand, within AZOBR_RS04255 at 898.324 kb on + strand, within AZOBR_RS04255 at 898.324 kb on + strand, within AZOBR_RS04255 at 898.325 kb on - strand, within AZOBR_RS04255 at 898.325 kb on - strand, within AZOBR_RS04255 at 898.325 kb on - strand, within AZOBR_RS04255 at 898.339 kb on + strand, within AZOBR_RS04255 at 898.378 kb on + strand, within AZOBR_RS04255 at 898.378 kb on + strand, within AZOBR_RS04255 at 898.498 kb on + strand, within AZOBR_RS04255 at 898.498 kb on + strand, within AZOBR_RS04255 at 898.498 kb on + strand, within AZOBR_RS04255 at 898.498 kb on + strand, within AZOBR_RS04255 at 898.498 kb on + strand, within AZOBR_RS04255 at 898.499 kb on - strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 899.298 kb on + strand at 899.299 kb on - strand at 899.330 kb on + strand at 899.402 kb on - strand at 899.402 kb on - strand at 899.412 kb on + strand at 899.412 kb on + strand at 899.413 kb on - strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.701 kb on - strand at 899.701 kb on - strand at 899.701 kb on - strand at 900.069 kb on + strand, within AZOBR_RS04265 at 900.069 kb on + strand, within AZOBR_RS04265
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 897,917 - AZOBR_RS04255 0.17 -1.0 897,917 - AZOBR_RS04255 0.17 -0.8 897,917 - AZOBR_RS04255 0.17 -0.7 898,075 + AZOBR_RS04255 0.34 +1.7 898,075 + AZOBR_RS04255 0.34 -0.3 898,076 - AZOBR_RS04255 0.34 -0.9 898,150 + AZOBR_RS04255 0.41 +2.0 898,150 + AZOBR_RS04255 0.41 -1.3 898,150 + AZOBR_RS04255 0.41 -0.6 898,150 + AZOBR_RS04255 0.41 +0.5 898,151 - AZOBR_RS04255 0.42 +0.9 898,151 - AZOBR_RS04255 0.42 -2.1 898,151 - AZOBR_RS04255 0.42 -1.0 898,151 - AZOBR_RS04255 0.42 -0.3 898,151 - AZOBR_RS04255 0.42 +1.6 898,151 - AZOBR_RS04255 0.42 -0.4 898,151 - AZOBR_RS04255 0.42 -3.0 898,324 + AZOBR_RS04255 0.59 +2.0 898,324 + AZOBR_RS04255 0.59 -0.7 898,325 - AZOBR_RS04255 0.60 -4.0 898,325 - AZOBR_RS04255 0.60 -2.7 898,325 - AZOBR_RS04255 0.60 -0.3 898,339 + AZOBR_RS04255 0.61 -0.4 898,378 + AZOBR_RS04255 0.65 -2.6 898,378 + AZOBR_RS04255 0.65 -0.2 898,498 + AZOBR_RS04255 0.77 +0.0 898,498 + AZOBR_RS04255 0.77 +0.1 898,498 + AZOBR_RS04255 0.77 +0.0 898,498 + AZOBR_RS04255 0.77 -3.5 898,498 + AZOBR_RS04255 0.77 +0.5 898,499 - AZOBR_RS04255 0.78 +1.6 898,576 + AZOBR_RS04255 0.86 -0.7 898,576 + AZOBR_RS04255 0.86 -1.7 898,576 + AZOBR_RS04255 0.86 -0.8 898,576 + AZOBR_RS04255 0.86 -2.6 898,576 + AZOBR_RS04255 0.86 -0.5 898,576 + AZOBR_RS04255 0.86 -0.4 898,576 + AZOBR_RS04255 0.86 -0.7 898,577 - AZOBR_RS04255 0.86 -0.1 898,577 - AZOBR_RS04255 0.86 +1.1 898,577 - AZOBR_RS04255 0.86 -0.1 898,577 - AZOBR_RS04255 0.86 -0.2 898,577 - AZOBR_RS04255 0.86 +0.0 898,577 - AZOBR_RS04255 0.86 -2.9 899,298 + -2.0 899,299 - -0.1 899,330 + -0.6 899,402 - +1.7 899,402 - -1.9 899,412 + +0.9 899,412 + -2.4 899,413 - -0.5 899,592 + +0.7 899,592 + -1.3 899,592 + +0.6 899,592 + -1.8 899,592 + -0.0 899,593 - +0.6 899,593 - +1.5 899,593 - -1.4 899,593 - -1.6 899,593 - +1.0 899,701 - -0.2 899,701 - -0.0 899,701 - -0.6 900,069 + AZOBR_RS04265 0.31 -0.1 900,069 + AZOBR_RS04265 0.31 +1.5
Or see this region's nucleotide sequence